Relationship Between Number of Grafted Queen Cell Cups and Amount of Produced Royal Jelly in Honey Bee Colonies Apis mellifera L.

AbstractAdding queen mandibular pheromone into honey bee colonies earlier than 24 h after queen loss resulted in an inhibition of queen-rearing, but not when added after 4 days. The number of queen cells initiated in each treatment decreased with the addition of the pheromone, although there were no effects on the number of queen cells torn down following pheromone treatment. The effect of adding the pheromone to queenless colonies given newly hatched female larvae under different regimens of queen cell provisioning and cell structure also was investigated. Only colonies in which larvae were presented in unmodified worker comb exhibited significantly lower rates of queen-rearing. Results indicate that queen mandibular pheromone inhibits the initiation of queen-rearing but not the maintenance of established cells.

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Effect of nutritional supplements on queen cell production in honey bee (Apis mellifera)

Journal of Applied and Natural Science ◽

10.31018/jans.v7i1.623 ◽

2015 ◽

Vol 7 (1) ◽

pp. 400-403

Author(s):

Nazia Haleem ◽

Neelima R. Kumar ◽

Rajinder Kaur

Keyword(s):

Apis Mellifera ◽

Honey Bee ◽

Honey Bees ◽

Nutritional Supplements ◽

Cell Production ◽

Queen Cell ◽

Important Means ◽

Second Year ◽

Bee Colonies

Honey bees are an important means of earning a living both at small and commercial levels. Maximum benefits can be obtained from strong colonies and in order to maintain strong colonies a good beekeeper requeens the colonies every second year. This requires a number of queens. The advances in beekeeping technology have made it possible to rear queens artificially or naturally. There is scope for improvement of these methods. The aim of the present study was to investigate if nutritional supplements could facilitate queen cell production in spring and autumn seasons. Becosule, thiamine, yeast and sugar solutions were fed to the honey bee colonies. The greatest number of queen cells was produced in the yeast fed colonies in spring. Bee mortality was observed in case of becosule. Perhaps the formulation contained some components which were toxic to honey bees. The effect on queen cell production by the different nutrients was in the order of Yeast > Thiamine > Becosule.

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The Transition Ratio of Nosema spp. Spores From Colonies to Honey Versus Honey to Colonies

Journal of Agricultural Science ◽

10.5539/jas.v11n1p72 ◽

2018 ◽

Vol 11 (1) ◽

pp. 72

Author(s):

Ceren Sarıbıyık ◽

Aslı Özkırım

Keyword(s):

Risk Assessment ◽

Apis Mellifera ◽

Honey Bee ◽

Honey Bees ◽

Digestive System ◽

Royal Jelly ◽

Nosema Apis ◽

Major Threat ◽

Bee Disease ◽

Bee Colonies

Honey bees (Apis mellifera L.) are insects that have an important role in plant pollination as well as production of benefical products such as honey, propolis, pollen, royal jelly, bee venom and beeswax. There is a growing interest in bee diseases and loss, which is a major threat to the economy and human health. Nosemosis is an adult honey bee disease which effects its digestive system mostly. The cause for the disease is Nosema apis or Nosema ceranae but the two can be seen together, too. This article aims to explore the transmission of Nosemosis and its effect on honey. For this purpose, a field study was conducted in Muğla province, where 51 pieces were collected from bee yards as spring samples and 51 pieces from bee yards as autumn samples during these two seasons, and 51 honey samples from bee yards were examined during the honey harvest. The results revealed that Nosema spp. which was obtained from honey bee samples collected in spring was more effective on honey samples. Nosema spp. was found to have a linear relation with the infection in the hive. It was determined that the percentage of Nosema spores seen in adults was 1.63%. This result contributed to the literature by providing this ratio used in estimating the level of infection in the colonies by means of honey sampling. Furthermore this is the first study where the contamination risk of honey from the infected colonies is calculated. Hopefully, this study can provide background for further research on the protection of bee colonies and risk assessment against Nosema spp. disease.

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Resistance to American foulbrood disease by honey bee colonies Apis mellifera bred for hygienic behavior

Apidologie ◽

10.1051/apido:2001103 ◽

2001 ◽

Vol 32 (6) ◽

pp. 555-565 ◽

Cited By ~ 155

Author(s):

Marla Spivak ◽

Gary S. Reuter

Keyword(s):

Apis Mellifera ◽

Honey Bee ◽

American Foulbrood ◽

Hygienic Behavior ◽

American Foulbrood Disease ◽

Bee Colonies

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Varroa jacobsoni infestation of adult Africanized and Italian honey bees (Apis mellifera) in mixed colonies in Brazil

Genetics and Molecular Biology ◽

10.1590/s1415-47571999000300006 ◽

1999 ◽

Vol 22 (3) ◽

pp. 321-323 ◽

Cited By ~ 12

Author(s):

Geraldo Moretto ◽

Leonidas João de Mello Jr.

Keyword(s):

Apis Mellifera ◽

Honey Bee ◽

Honey Bees ◽

Mite Infestation ◽

Varroa Jacobsoni ◽

Africanized Honey Bees ◽

African Honey Bees ◽

Africanized Bees ◽

Bee Colonies ◽

Different Levels

Different levels of infestation with the mite Varroa jacobsoni have been observed in the various Apis mellifera races. In general, bees of European races are more susceptible to the mite than African honey bees and their hybrids. In Brazil honey bee colonies are not treated against the mite, though apparently both climate and bee race influence the mite infestation. Six mixed colonies were made with Italian and Africanized honey bees. The percentage infestation by this parasite was found to be significantly lower in adult Africanized (1.69 ± 0.44) than Italian bees (2.79 ± 0.65). This ratio was similar to that found in Mexico, even though the Africanized bees tested there had not been in contact with varroa, compared to more than 20 years of the coexistence in Brazil. However, mean mite infestation in Brazil on both kinds of bees was only about a third of that found in Mexico.

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The effect of the queen's age on the Varroa mite (Varroa destructor) burden of honey bee (Apis mellifera L.) colonies

Acta Agraria Debreceniensis ◽

10.34101/actaagrar/75/1651 ◽

2018 ◽

pp. 83-87

Author(s):

Marianna Takács ◽

János Oláh

Keyword(s):

Apis Mellifera ◽

Honey Bee ◽

Varroa Destructor ◽

Early Spring ◽

Brood Chamber ◽

One Year ◽

The One ◽

Post Hoc ◽

Bee Colonies ◽

Mite Mortality

An apiary trial was conducted in 2016 August to October in Szabolcs-Szatmár-Bereg County, Nyírmada to evaluate the influence of queen’s age on the Varroa destructor-burden in the treatment colonies. Sixty colonies of bees belonging to the subspecies Apis mellifera carnica pannonica in Hunor loading hives (with 10 frames in the brood chamber/deep super) were used. The colonies were treated with amitraz and the organophosphate pesticide coumaphos active ingredients. The amitraz treatment includes 6 weeks. The coumaphos treatment with Destructor 3.2% can be used for both diagnosis and treatment of Varroasis. For diagnosis, one treatment is sufficient. For control, two treatments at an interval of seven days are required. The colonies were grouped by the age of the queen: 20 colonies with one-year-old, 20 colonies with two-year-old and 20 colonies with three-year-old queen. The mite mortality of different groups was compared. The number of fallen mites was counted at the white bottom boards. The examination of spring growth of honey bee colonies has become necessary due to the judgement of efficiency of closing treatment. The data was recorded seven times between 16th March 2017 and 19th May 2017. Data on fallen mites were subjected to one-way analysis of variance (ANOVA) and Post-Hoc Tukey-test. Statistical analysis was performed using the software of IBM SPSS (version 21.). During the first two weeks after treatments, the number of fallen mites was significantly higher in the older queen’s colonies (Year 2014). The total mite mortality after amitraz treatment in the younger queen’s colonies was lower (P<0.05) compared to the three-year-old queen’s colonies. According to Takács and Oláh (2016) although the mitemortality tendency, after the coumaphos (closing) treatment in colonies which have Year 2014 queen showed the highest rate, considering the mite-burden the colonies belongs to the average infected category. The colonial maintenance ability of three-year-old queen cannot be judged based on the influencing effect on the mite-burden. The importance of the replacement of the queen was judged by the combined effect of several factors. During the spring-growth study (16th March–19th May) was experienced in the three-year-old queen’s colonies the number of brood frames significantly lower compared to the one- and two-year-old queen’s colonies. In the study of 17th April and 19th May each of the three queen-year-groups were varied. Therefore in the beekeeping season at different times were determined the colonial maintenance ability of queens by more factors: efficiency of closing treatment in early spring, the spring-growth of bee colonies, the time of population shift (in current study, this time was identical in each queen-year), honey production (from black locust).

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Implementasi Honey Bee Mating Optimization Pada Vehicle Routing Problem With Time Windows Dalam Perencanaan Jalur Wisata Malang

Repositor ◽

10.22219/repositor.v2i4.392 ◽

2020 ◽

Vol 2 (4) ◽

pp. 495

Author(s):

M Syawaluddin Putra Jaya ◽

Yufiz Azhar ◽

Nur Hayatin

Keyword(s):

Honey Bee ◽

Royal Jelly ◽

Time Windows ◽

Route Planning ◽

Routing Problem ◽

Capacity Limit ◽

Shortest Route ◽

Mutation Ratio ◽

Bee Colonies ◽

Honey Bee Mating Optimization

Abstrak Vahicle Routing Problem adalah suatu masalah pencaian jalur yang akan dilalui dengan tujuan mencari rute yang paling cepat atau pendek. Vahicle Routing Problem with Time Windows (VRPTW) yang merupakan sebutan bagi VRP dengan kendala tambahan berupa adanya time windows pada masing-masing pelanggan yang dalam hal ini berupa destinasi wisata. Dalam penelitian ini diterapkan Honey Bee Mating Optimization (HBMO) dalam menyelesaikan VRPTW. HBMO sendiri terinspirasi oleh perilaku koloni lebah ketika bereproduksi. Algoritma tersebut bertujuan untuk mengevaluasi pencarian individu atau solusi terbaik. Tujuan dari penelitian ini adalah bagaimana mengimplementasikan Honey Bee Mating Optimization dalam menyelesaikan VRPTW pada perencanaan jalur wisata di Malang. Sehingga dapat meminimumkan waktu dan jarak tempuh perjalanan. Berdasarkan hasil pengujian, parameter yang optimal untuk optimasi VRPTW menggunakan HBMO pada kasus perencannan jalur wisata Malang yaitu dengan menggunakan 800 generasi, populasi lebah jantan sebesar 300, batas kapasitas spermatheca sejumlah 100, nilai mutation ratio (Pm) dan royal jelly masing-masing bernilai 0.5.Abstract Vahicle Routing Problem is a problem of finding the best route that will be passed with the purpose to finding the fastest or shortest route. Vahicle Routing Problem with Time Windows (VRPTW) is a part of VRP with additional obstacles in the form of time windows in each customer. In this research, Honey Bee Mating Optimization (HBMO) was applied to completing VRPTW. HBMO itself was inspired by the behavior of bee colonies when reproducing. The purpose of this algorithm is to evaluate the best individual or the best solutions. The purpose of this research is how to implement Honey Bee Mating Optimization to completing VRPTW in Malang tourism route planning. So that it can minimize travel time and distance. Based on the results of the testing, the optimal parameters for VRPTW optimization using HBMO in Malang tourism route planning case are using 800 generations, the male bee population is 300, the capacity limit of spermatheca is 100, the mutation ratio (Pm) and royal jelly are respectively 0.5.

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Transcriptional Control of Honey Bee (Apis mellifera) Major Royal Jelly Proteins by 20-Hydroxyecdysone

Insects ◽

10.3390/insects9030122 ◽

2018 ◽

Vol 9 (3) ◽

pp. 122 ◽

Cited By ~ 4

Author(s):

Paul Winkler ◽

Frank Sieg ◽

Anja Buttstedt

Keyword(s):

Gene Expression ◽

Apis Mellifera ◽

Juvenile Hormone ◽

Honey Bee ◽

Honey Bees ◽

Transcriptional Control ◽

Royal Jelly ◽

Adult Worker ◽

Gene Expression Dynamics

One of the first tasks of worker honey bees (Apis mellifera) during their lifetime is to feed the larval offspring. In brief, young workers (nurse bees) secrete a special food jelly that contains a large amount of unique major royal jelly proteins (MRJPs). The regulation of mrjp gene expression is not well understood, but the large upregulation in well-fed nurse bees suggests a tight repression until, or a massive induction upon, hatching of the adult worker bees. The lipoprotein vitellogenin, the synthesis of which is regulated by the two systemic hormones 20-hydroxyecdysone and juvenile hormone, is thought to be a precursor for the production of MRJPs. Thus, the regulation of mrjp expression by the said systemic hormones is likely. This study focusses on the role of 20-hydroxyecdysone by elucidating its effect on mrjp gene expression dynamics. Specifically, we tested whether 20-hydroxyecdysone displayed differential effects on various mrjps. We found that the expression of the mrjps (mrjp1–3) that were finally secreted in large amounts into the food jelly, in particular, were down regulated by 20-hydroxyecdysone treatment, with mrjp3 showing the highest repression value.

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