scholarly journals Evaluation of in vitro antioxidant activities and total phenol content of different extracts of the fungus Ulocladium chartarum isolated from Draceana leaf

2018 ◽  
Vol 1 (2) ◽  
pp. 75-77
Author(s):  
Nada Abdel-Wahab ◽  
Eman Attia ◽  
Samar Desoukey ◽  
Ehab Elkhayat ◽  
Mostafa Fouad ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content

Total Phenol Content, Antioxidant Activities and α-Glucosidase Inhibition of Sesame Cake Extracts

2012 ◽  
Vol 37 (6) ◽  
pp. 723-731 ◽  
Author(s):  
M.V. Reshma ◽  
L.K. Namitha ◽  
A. Sundaresan ◽  
Challa Ravi Kiran
Keyword(s):  
Antioxidant Activities ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Sesame Cake

Antioxidant Capacity and Polyphenolic Content of Eucommia Ulmoides Oliv Leaf Extract

2011 ◽  
Vol 396-398 ◽  
pp. 1349-1352
Author(s):  
Jian Hui Wang ◽  
Dong Min Liu ◽  
Yong Le Liu ◽  
Chi Ling Li ◽  
Yuan Yuan Cheng ◽  
...  
Keyword(s):  
Vegetable Oils ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenol ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Phenol Content ◽  
Dpph Method

This study was designed to determine the polyphenolic contents of Eucommia ulmoicles Oliv leaf extracts and evaluate their antioxidant activities in vitro. All the extracts were evaluated for their radical scavenging activity by the DPPH method and their oxidative-retarding efficiency in vegetable oils. It was observed that the highest values were found in 70% MeOH extracts for total phenolic content as gallic acid. A good correlation between antioxidant activity and total phenol content in the extracts was found. The extraction yield, total phenol content and radical scavenging activity were increased and peroxide formation in vegetable oils were decreased with the graded solvent concentration of MeOH. The phenolic compound yield ranged from 2.45 to 11.35% and the total phenol content increased from 34.51 mg/g to 47.68 mg/g. DPPH scavenging activity increased from 56.32% to 90.37% as MeOH concentration increased. Compared to the control (70.45 meq/kg), 70% MeOH fraction with 47.68 mg/g phenolic compounds shows much stronger antioxidant ability (30.12 meq/kg) in vegetable oil system.

scholarly journals Comparative Analysis of the Nutritional Components and Antioxidant Activities of Different Brassica juncea Cultivars

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 840
Author(s):  
Hee-Yeon Kwon ◽  
Sun-Il Choi ◽  
Hye-In Park ◽  
Seung-Hyun Choi ◽  
Wan-Sup Sim ◽  
...  
Keyword(s):  
Brassica Juncea ◽  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Crude Lipid ◽  
Antioxidant Power ◽  
Nutritional Components

The purpose of this study was to compare the nutritional components and antioxidant activities of two different cultivars of Brassica juncea (Dolsan, Yeosu, Korea (BJD) and (Jeongseon, Gangwon, Korea (BJJ)). We investigated the proximate composition (moisture, crude ash, crude protein and crude lipid), antioxidant activities (2,2-Diphenyl-2-picrylhydrazil (DPPH) scavenging activity and ferric reducing antioxidant power (FRAP)), total phenol content, total flavonoid content and sinigrin content by high-performance liquid chromatography analysis. Our results show that the proximate compositions of BJD and BJJ were not significantly different. However, both the DPPH radical scavenging and FRAP activities of the BJJ extracts were higher than those of the BJD extracts. The total phenol contents of the BJD and BJJ extracts were 6.56 and 9.80 mg gallic acid equivalent/g, respectively. The total flavonoids content of the BJD and BJJ extracts were 20.92 and 34.81 mg rutin equivalent/g, respectively, whereas the sinigrin contents, one of the major compounds in BJD and BJJ extracts, were 16.16 mg/g and 11.73 mg/g, respectively. In this study, we confirmed that, by comparing BJJ and BJD, the sinigrin content of BJD was higher than that of BJJ, but the antioxidant activity and phenol content of BJD were superior to that of BJJ.

Total phenol content of guava fruit and development of an in vitro regeneration protocol amenable to genetic improvement

2011 ◽  
Vol 4 (2/3/4) ◽  
pp. 225 ◽  
Author(s):  
Viji Sitther ◽  
Donna L. Harris ◽  
Sadanand A. Dhekney ◽  
Jinhe Bai ◽  
Elizabeth A. Baldwin ◽  
...  
Keyword(s):  
Genetic Improvement ◽  
In Vitro Regeneration ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Guava Fruit

scholarly journals Phytochemical Characterization, In Vitro Antioxidant, Cytotoxic, and Antibacterial Effects of Aristolochia longa L.,

2020 ◽  
Vol 11 (1) ◽  
pp. 8129-8140
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Colorimetric Method ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phytochemical Screening ◽  
Phenol Content

The aim of this study was devoted, in the one hand, to determine a preliminary phytochemical screening of methanolic and aqueous root extracts of Aristolochia longa L. In the other hand, polyphenols and flavonoids contents, in vitro antioxidant, antitumor, and antibacterial activities of the organic extract of A. longa were determined. Phytochemical screening was carried out using standard methods of precipitation and coloration reactions. The total phenol content and flavonoid contents were determined using the Folin-Ciocalteu method and Aluminum chloride colorimetric method, respectively. Antioxidant activity was studied using DPPH assay. The cytotoxic activity of extracts was evaluated against three cancerous cell lines using MTT assay, and antibacterial activity was tested against Escherichia coli, Pseudomonas aeruginosa, Rhodococcus equi, and Staphylococcus aureus using the agar well diffusion assay. Preliminary phytochemical screening for aqueous and ethanolic extracts revealed the presence of starch, tannins, flavonoids, coumarins, and anthocyanins. The total phenol content of A. longa extracts showed that the methanol extract has the highest polyphenol and flavonoid concentrations with 101.4 mg GAE (Gallic acid equivalent)/g and 54.21 mg QE (Quercetin equivalent)/g extract, respectively. The methanolic extract also exhibited the highest antioxidant capacity of DPPH (IC50 = 1.32 mg/mL). While hexane extract exhibited both a potent inhibitory effect on VERO cell growth with IC50 = 15.125 μg/mL and a total inhibitory effect on Staphylococcus aureus with inhibition zone 8.5 mm. The high content of polyphenols in A. longa explains their antioxidant, antitumor, and antibacterial activity. Thus, this plant could be a significant source of natural compounds in preventing the development of cancer.

scholarly journals BIOACTIVITIES OF THE ETHYL ACETATE EXTRACT OF RHODOCOCCUS QINGSHENGII STRAIN BJC15-A38 AN ENDOPHYTE OF AZADIRACHTA INDICA A. JUSS

2017 ◽  
Vol 9 (6) ◽  
pp. 211 ◽  
Author(s):  
Preeti Saini ◽  
Madhurama Gangwar ◽  
Amrinder Kaur
Keyword(s):  
Ethyl Acetate ◽  
Azadirachta Indica ◽  
Significant Inhibition ◽  
Total Phenol ◽  
Antidiabetic Activity ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Alpha Glucosidase ◽  
Antidiabetic Effects

Objective: This study evaluated the antidiabetic activities of the ethyl acetate-methanol (4:1) extract (EA) of Rhodococcus qingshengii strain BJC15-A38 supernatant.Methods: In vitro alpha-amylase and alpha-glucosidase inhibitory assays, were used to determine the antidiabetic activity of EA (100, 250, 500, 750 and 1000 μg/ml). Also, the total phenol content of the extract was determined, followed by understanding the mode of α-amylase inhibition.Results: The crude extract from Azadirachta indica A. Juss. endophytic actinomycete Rhodococcus qingshengii strain BJC15-A38 was found to have significant inhibition against α-amylase (IC50 = 65.93 µg/ml) and α-glucosidase (IC50 = 929.56 µg/ml). The total phenol content of the crude was found to be 97.25±6.47 and 15.40±0.43 mg/g of the extract in terms of catechol and gallic acid equivalents respectively. Purification of the extract was carried out using silica gel column chromatography. Kinetic analysis revealed the mixed competitive with mixed competitive of inhibition towards α-amylase.Conclusion: The findings suggest that the extract of Rhodococcus qingshengii strain BJC15-A38 possesses antidiabetic effects.

scholarly journals The potential of Endophytic Fungal Extract Isolated from Cinnamon (Cinnamomum burmannii) as Antidiabetic and Antioxidant

2019 ◽  
Vol 22 (6) ◽  
pp. 275-282 ◽  
Author(s):  
Eris Septiana ◽  
Fauzy Rachman ◽  
Yatri Hapsari ◽  
Yadi Yadi ◽  
Bustanussalam Bustanussalam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Endophytic Fungi ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Total Phenol ◽  
Antidiabetic Activity ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Fungal Extract ◽  
Glucose Levels

An increase in blood glucose levels in people with diabetes can cause an increase in free radicals, which can worsen the disease. Thus, drugs that have antidiabetic and antioxidant activities are needed. The cinnamon plant is high in antioxidants and has long been used as a source for a diabetes drug. The utilization of endophytic fungi isolated from cinnamon plants as antidiabetic and antioxidant has never been reported. This study aims to investigate the antidiabetic as well as antioxidant activity from the extract of endophytic fungi from the cinnamon plant. The antidiabetic activity was tested using the α-glucosidase enzyme inhibition method, while antioxidant activity was tested using the DPPH free radical scavenging method. Total phenol content was measured based on the Follin-Ciocalteu reagent reaction. All endophytic fungal extracts from the cinnamon leaves, twigs, flowers, and fruit have antidiabetic and antioxidant activity as well as high total phenol content. The three parameters measured showed a positive correlation. Endophytic fungal extract of Cb.D6 isolate derived from the leaf had the highest antidiabetic and antioxidant activity among the other isolates amounting to 92.41% and 90.28%, respectively. In addition, the total phenol content of Cb.D6 isolates was also the highest with 357.83 mg equivalent to gallic acid/g extract. Therefore, the endophytic fungal extract of Cb.D6 isolate has the potential to be developed as a source of the antidiabetic and antioxidant ingredients.

scholarly journals Antioxidant activities and total phenol content of Inula viscosa extracts selected from three regions of Morocco

2015 ◽  
Vol 5 (3) ◽  
pp. 228-233 ◽  
Author(s):  
Naima Chahmi ◽  
Jaouad Anissi ◽  
Sanae Jennan ◽  
Abdellah Farah ◽  
Khalid Sendide ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content

scholarly journals Total Phenol Content and In Vitro Antioxidant Potential of Helicanthus elastica (Desr.) Danser-A Less-explored Indian Mango Mistletoe

2014 ◽  
Vol 4 (4) ◽  
pp. 285-288 ◽  
Author(s):  
Koppala Narayana Sunil Kumar ◽  
Ariyamuthu Saraswathy ◽  
Swaminathan Amerjothy ◽  
Thomas Susan ◽  
Basaviah Ravishankar
Keyword(s):  
Total Phenol ◽  
Antioxidant Potential ◽  
Total Phenol Content ◽  
Phenol Content

In-vitro Antioxidant and Cytotoxic properties of Strobilanthes kunthianus

2021 ◽  
pp. 2522-2528
Author(s):  
Geetha Balasubramaniam ◽  
Mahendran Sekar ◽  
Shrishailappa Badami
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Cytotoxic Activities ◽  
Phenol Content ◽  
Methanol Extracts ◽  
Flower Extract ◽  
Hela Cell Lines

Background: Strobilanthes kunthianus Nees T Anders (S. kunthianus, Neela kurinji) is a shrub in the grasslands of Western Ghats in India. The Nilgiris, which literally means the blue mountains got its name from the purplish blue flowers of Neelakurinji that blossoms gregariously once in twelve years. It is well known for its biological properties. However, so far there is no detailed antioxidant and cytotoxic studies has been carried out. Objective: The present study aimed to evaluate in-vitro antioxidant and cytotoxic activities of various parts of S. kunthianus extracts. Methods: Fourteen extracts were prepared in different parts of S. kunthianus including stem, leaves, flowers and roots. All the extracts were examined for in-vitro antioxidant and cytotoxic activities using standard methods. The total phenol content was estimated by Folin Ciocalteau method. Results: The crude methanol flower extract was found to contain very high total phenol content among all the extracts. Among the tested extracts, the ethyl acetate extracts of root and stem showed potent in-vitro antioxidant activity, when compared to ascorbic acid in ABTS method. These extracts showed better activity than other successive extracts of root and stem. In case of methanol extracts, the crude extracts showed potent antioxidant activity in ABTS, H2O2, and total antioxidant capacity methods. The crude methanol flower extract was found to be the most potent among the methanol extracts. Many natural products have served as anticancer agents in the treatment and also as lead compounds for further research. All the extracts were tested for their cytotoxic activity against Hep-2 and HeLa cell lines. But all the extracts showed weak activity in the tested cell lines. Conclusion: S. kunthianus merits further investigation in animal models to confirm its antioxidant and anticancer properties and to isolate the phytoconstituents that are responsible for their biological activities.

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