Total phenol content of guava fruit and development of an in vitro regeneration protocol amenable to genetic improvement

2011 ◽  
Vol 4 (2/3/4) ◽  
pp. 225 ◽  
Author(s):  
Viji Sitther ◽  
Donna L. Harris ◽  
Sadanand A. Dhekney ◽  
Jinhe Bai ◽  
Elizabeth A. Baldwin ◽  
...  
Keyword(s):  
Genetic Improvement ◽  
In Vitro Regeneration ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Guava Fruit

scholarly journals Phytochemical Characterization, In Vitro Antioxidant, Cytotoxic, and Antibacterial Effects of Aristolochia longa L.,

2020 ◽  
Vol 11 (1) ◽  
pp. 8129-8140
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Colorimetric Method ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phytochemical Screening ◽  
Phenol Content

The aim of this study was devoted, in the one hand, to determine a preliminary phytochemical screening of methanolic and aqueous root extracts of Aristolochia longa L. In the other hand, polyphenols and flavonoids contents, in vitro antioxidant, antitumor, and antibacterial activities of the organic extract of A. longa were determined. Phytochemical screening was carried out using standard methods of precipitation and coloration reactions. The total phenol content and flavonoid contents were determined using the Folin-Ciocalteu method and Aluminum chloride colorimetric method, respectively. Antioxidant activity was studied using DPPH assay. The cytotoxic activity of extracts was evaluated against three cancerous cell lines using MTT assay, and antibacterial activity was tested against Escherichia coli, Pseudomonas aeruginosa, Rhodococcus equi, and Staphylococcus aureus using the agar well diffusion assay. Preliminary phytochemical screening for aqueous and ethanolic extracts revealed the presence of starch, tannins, flavonoids, coumarins, and anthocyanins. The total phenol content of A. longa extracts showed that the methanol extract has the highest polyphenol and flavonoid concentrations with 101.4 mg GAE (Gallic acid equivalent)/g and 54.21 mg QE (Quercetin equivalent)/g extract, respectively. The methanolic extract also exhibited the highest antioxidant capacity of DPPH (IC50 = 1.32 mg/mL). While hexane extract exhibited both a potent inhibitory effect on VERO cell growth with IC50 = 15.125 μg/mL and a total inhibitory effect on Staphylococcus aureus with inhibition zone 8.5 mm. The high content of polyphenols in A. longa explains their antioxidant, antitumor, and antibacterial activity. Thus, this plant could be a significant source of natural compounds in preventing the development of cancer.

scholarly journals BIOACTIVITIES OF THE ETHYL ACETATE EXTRACT OF RHODOCOCCUS QINGSHENGII STRAIN BJC15-A38 AN ENDOPHYTE OF AZADIRACHTA INDICA A. JUSS

2017 ◽  
Vol 9 (6) ◽  
pp. 211 ◽  
Author(s):  
Preeti Saini ◽  
Madhurama Gangwar ◽  
Amrinder Kaur
Keyword(s):  
Ethyl Acetate ◽  
Azadirachta Indica ◽  
Significant Inhibition ◽  
Total Phenol ◽  
Antidiabetic Activity ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Alpha Glucosidase ◽  
Antidiabetic Effects

Objective: This study evaluated the antidiabetic activities of the ethyl acetate-methanol (4:1) extract (EA) of Rhodococcus qingshengii strain BJC15-A38 supernatant.Methods: In vitro alpha-amylase and alpha-glucosidase inhibitory assays, were used to determine the antidiabetic activity of EA (100, 250, 500, 750 and 1000 μg/ml). Also, the total phenol content of the extract was determined, followed by understanding the mode of α-amylase inhibition.Results: The crude extract from Azadirachta indica A. Juss. endophytic actinomycete Rhodococcus qingshengii strain BJC15-A38 was found to have significant inhibition against α-amylase (IC50 = 65.93 µg/ml) and α-glucosidase (IC50 = 929.56 µg/ml). The total phenol content of the crude was found to be 97.25±6.47 and 15.40±0.43 mg/g of the extract in terms of catechol and gallic acid equivalents respectively. Purification of the extract was carried out using silica gel column chromatography. Kinetic analysis revealed the mixed competitive with mixed competitive of inhibition towards α-amylase.Conclusion: The findings suggest that the extract of Rhodococcus qingshengii strain BJC15-A38 possesses antidiabetic effects.

scholarly journals Total Phenol Content and In Vitro Antioxidant Potential of Helicanthus elastica (Desr.) Danser-A Less-explored Indian Mango Mistletoe

2014 ◽  
Vol 4 (4) ◽  
pp. 285-288 ◽  
Author(s):  
Koppala Narayana Sunil Kumar ◽  
Ariyamuthu Saraswathy ◽  
Swaminathan Amerjothy ◽  
Thomas Susan ◽  
Basaviah Ravishankar
Keyword(s):  
Total Phenol ◽  
Antioxidant Potential ◽  
Total Phenol Content ◽  
Phenol Content

In-vitro Antioxidant and Cytotoxic properties of Strobilanthes kunthianus

2021 ◽  
pp. 2522-2528
Author(s):  
Geetha Balasubramaniam ◽  
Mahendran Sekar ◽  
Shrishailappa Badami
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Cytotoxic Activities ◽  
Phenol Content ◽  
Methanol Extracts ◽  
Flower Extract ◽  
Hela Cell Lines

Background: Strobilanthes kunthianus Nees T Anders (S. kunthianus, Neela kurinji) is a shrub in the grasslands of Western Ghats in India. The Nilgiris, which literally means the blue mountains got its name from the purplish blue flowers of Neelakurinji that blossoms gregariously once in twelve years. It is well known for its biological properties. However, so far there is no detailed antioxidant and cytotoxic studies has been carried out. Objective: The present study aimed to evaluate in-vitro antioxidant and cytotoxic activities of various parts of S. kunthianus extracts. Methods: Fourteen extracts were prepared in different parts of S. kunthianus including stem, leaves, flowers and roots. All the extracts were examined for in-vitro antioxidant and cytotoxic activities using standard methods. The total phenol content was estimated by Folin Ciocalteau method. Results: The crude methanol flower extract was found to contain very high total phenol content among all the extracts. Among the tested extracts, the ethyl acetate extracts of root and stem showed potent in-vitro antioxidant activity, when compared to ascorbic acid in ABTS method. These extracts showed better activity than other successive extracts of root and stem. In case of methanol extracts, the crude extracts showed potent antioxidant activity in ABTS, H2O2, and total antioxidant capacity methods. The crude methanol flower extract was found to be the most potent among the methanol extracts. Many natural products have served as anticancer agents in the treatment and also as lead compounds for further research. All the extracts were tested for their cytotoxic activity against Hep-2 and HeLa cell lines. But all the extracts showed weak activity in the tested cell lines. Conclusion: S. kunthianus merits further investigation in animal models to confirm its antioxidant and anticancer properties and to isolate the phytoconstituents that are responsible for their biological activities.

A Potent In Vitro α-Amylase Inhibitory Action of Haplophyllum tuberculatum Extracts

2020 ◽  
Vol 15 (3) ◽  
pp. 190-196 ◽  
Author(s):  
Housseyn Chaoua ◽  
Ihcen Khacheba ◽  
Hadjer Boussoussa ◽  
Abir Bekhaoua ◽  
Mohamed Yousfi
Keyword(s):  
Inhibitory Activity ◽  
Inhibitory Action ◽  
Dry Matter ◽  
Total Phenol ◽  
Biologically Active ◽  
Antidiabetic Activity ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Haplophyllum Tuberculatum

Background: Natural plant active compounds were found to inhibit the activity of several enzymes that may be related to several diseases. Objective: This study aimed at testing the antidiabetic activity related to the phenol content by in vitro α-Amylase inhibitory action effect of aqueous, organic and essential oil extracts of Haplophyllum tuberculatum, collected in the town of Laghouat in the steppe region of Algeria. Methods: Two types of aqueous extracts were prepared: Decoction and Diffusion extracts. The organic extracts were prepared with successful maceration in hexane, dichloromethane, ethyl acetate, ethanol and methanol. Also, essential oils were obtained by hydrodistillation. The analysis of the total Phenol content of our extracts was done with Folin-Ciocalteu reagent, as the flavonoid content was obtained in mixture with aluminum trichloride. The effects of the plant extracts on the catalytic efficiency of α-amylase enzyme were represented by the enzymatic inhibitory percentage of each extract in which the inhibitory activity was expressed as IC50. Results: The total phenol content showed values ranging between 0.27 and 11.97 mg gallic acid equivalent / g dry matter. The flavonoid contents vary from 0.05 to 1.50 mg equivalent of rutin /g of dry matter. All the extracts showed good inhibitory activity against α- amylase of IC50, values ranged from 0.05 to 50.03 mg/ml. Conclusion : This study reports for the first time the inhibitory capacity of Algerian Haplophyllum tuberculatum species against α-amylase which could provide natural biologically active agents to be used in the management of diabetes.

Evaluation of virgin olive oil bitterness by total phenol content analysis

2007 ◽  
Vol 109 (3) ◽  
pp. 193-197 ◽  
Author(s):  
Gabriel Beltrán ◽  
María T. Ruano ◽  
Antonio Jiménez ◽  
Marino Uceda ◽  
María P. Aguilera
Keyword(s):  
Content Analysis ◽  
Olive Oil ◽  
Virgin Olive Oil ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content

scholarly journals Total Phenol Content and Antimicrobial Activity of Lingonberry (Vaccinium vitis-idaea L.) from Several Areas in the Eastern Carpathians

2017 ◽  
Vol 9 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Éva LASLO ◽  
Zoltán Attila KÖBÖLKUTI
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Effect ◽  
Bioactive Compound ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Eastern Carpathians ◽  
Spoilage Bacteria ◽  
New Perspective ◽  
Similar Growth

This study evaluated the antimicrobial activity and the total phenol content of Vaccinium vitis idaea L. berry fruit from five different localities with distinct growth sites in the Eastern Carpathians. The antibacterial effect of lingonberry was studied on nine selected Gram-positive and negative, foodborne, illness causing and spoilage bacteria. The total phenol content was estimated by the Folin-Ciocalteu method. The present results showed stronger antibacterial effect of lingonberry on Gram-negative bacteria, especially on Pseudomonas fluorescens and Pseudomonas aeruginosa. The total phenol content varied between 3.72 and 2.1 mM GAE/ml. As data suggested, Vaccinium vitis-idaea fruits originating from different geographic regions and environment, differ from each other in terms of bioactive compound quantity and activity. In the selection of new perspective cultivars of lingonberry, the geographical origin of fruits must be considered. Two Step Cluster analysis detected relatively well supported relationship between samples provided from similar growth sites. Correlation analysis showed no correlation between altitude, phenol content and antimicrobial activity.

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