Influence of the origin of the inoculum on the anaerobic biodegradability test

2004 ◽  
Vol 49 (1) ◽  
pp. 53-59 ◽  
Author(s):  
I. Moreno-Andrade ◽  
G. Buitrón
Keyword(s):  
Most Probable Number ◽  
Microbial Composition ◽  
Probable Number ◽  
Methanogenic Activity ◽  
Sulfate Reducing ◽  
Anaerobic Biodegradability ◽  
Specific Methanogenic Activity ◽  
Solids Content ◽  
Different Sources ◽  
Selection Of

Five different sources of inocula were studied to determine its influence on biodegradability tests. Inocula were characterized determining granulometry, specific methanogenic activity, solids content, and volumetric sludge index. Also, the fermentative, aceticlastic, hydrogenophilic, OPHA, and sulfate-reducing groups were determined by the most probable number technique. Anaerobic biodegradability tests were conducted with two different substrates, one easy to degrade (glucose) and a toxic one (phenol). The best performance, in terms of percent of biodegradation and lag time, for both substrates, was obtained with the inoculum from a brewery industry UASB. The results can be explained in terms of the initial activity of the inoculum. The influence of the significant variations found in the specific methanogenic activity of the five inocula studied is discussed, in terms of the microbial composition of the samples. The results emphasized the importance of the selection of an appropriate source of inoculum in order to obtain reliable results.

scholarly journals Improving Biomethanation of Chicken Manure by Co-Digestion with Ethanol Plant Effluent

2019 ◽  
Vol 16 (24) ◽  
pp. 5023 ◽  
Author(s):  
Dae-Yeol Cheong ◽  
Jeffrey Todd Harvey ◽  
Jinsu Kim ◽  
Changsoo Lee
Keyword(s):  
Loading Rate ◽  
Chicken Manure ◽  
Continuous Reactor ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Methanogenic Activity ◽  
Volatile Solids ◽  
Methane Production Rate ◽  
Specific Methanogenic Activity ◽  
Ethanol Plant

As the global production of chicken manure has steadily increased, its proper management has become a challenging issue. This study examined process effluent from a bioethanol plant as a co-substrate for efficient anaerobic digestion of chicken manure. An anaerobic continuous reactor was operated in mono- and co-digestion modes by adding increasing amounts of the ethanol plant effluent (0%, 10%, and 20% (v/v) of chicken manure). Methanogenic performance improved significantly in terms of both methane production rate and yield (by up to 66% and 36%, respectively), with an increase in organic loading rate over the experimental phases. Correspondingly, the specific methanogenic activity was significantly higher in the co-digestion sludge than in the mono-digestion sludge. The reactor did not suffer any apparent process imbalance, ammonia inhibition, or nutrient limitation throughout the experiment, with the removal of volatile solids being stably maintained (56.3–58.9%). The amount of ethanol plant effluent appears to directly affect the rate of acidification, and its addition at ≥20% (v/v) to chicken manure needs to be avoided to maintain a stable pH. The overall results suggest that anerobic co-digestion with ethanol plant effluent may provide a practical means for the stable treatment and valorization of chicken manure.

Anaerobic biodegradation of 2,4,6-trichlorophenol by methanogenic granular sludge: role of co-substrates and methanogenic inhibition

2009 ◽  
Vol 59 (7) ◽  
pp. 1449-1456 ◽  
Author(s):  
D. Puyol ◽  
A. F. Mohedano ◽  
J. L. Sanz ◽  
J. J. Rodríguez
Keyword(s):  
Volatile Fatty Acids ◽  
Removal Rate ◽  
Granular Sludge ◽  
Anaerobic Biodegradation ◽  
Methanogenic Activity ◽  
The Third ◽  
Specific Methanogenic Activity ◽  
Methanol Yeast ◽  
Complete Dechlorination

The influence of several co-substrates in the anaerobic biodegradation of 2,4,6-trichlorophenol (246TCP) by methanogenic granular sludge as well as in methanogenesis inhibition by 246TCP has been studied. 4 g-COD·L−1 of lactate, sucrose, volatile fatty acids (VFA) acetate:propionate:butyrate 1:1:1, ethanol, methanol, yeast extract (YE), and 2 g-COD·L−1 of formate and methylamine were tested. Two concentrations of 246TCP: 80 mg·L−1 and 113 mg·L−1 (this last corresponding to the EC50 for acetotrophic methanogenesis) were tested. Three consecutive co-substrate and nutrient feedings were accomplished. 246TCP was added in the second feed, and the 246TCP removal rate increased considerably after the third feed. Accumulated metabolites after ortho-dechlorination, either 4-chlorophenol (4CP) (when methanol, ethanol or VFA were used as co-substrates) or 2,4-dichlorophenol (24DCP) (with lactate) avoided the complete dechlorination of 246TCP. With methylamine and formate this compound was degraded only partially. Monochlorophenols biodegradation was partially achieved with YE, but both 24DCP and 2,6-dichlorophenol (26DCP) were accumulated. In the presence of sucrose para-dechlorination was observed. 246TCP was better tolerated by methanogens when ethanol and methanol were added because of the highest specific methanogenic activity achieved with these co-substrates. Methanol and ethanol were the best co-substrates in the anaerobic biodegradation of 246TCP.

The development of a new methodology for the assessment of specific methanogenic activity

1990 ◽  
Vol 24 (7) ◽  
pp. 813-825 ◽  
Author(s):  
A James ◽  
C.A.L Chernicharo ◽  
C.M.M Campos
Keyword(s):  
Methanogenic Activity ◽  
Specific Methanogenic Activity

Optimization of the thermophilic anaerobic co-digestion of pig manure, agriculture waste and inorganic additive through specific methanogenic activity

2014 ◽  
Vol 69 (12) ◽  
pp. 2381-2388 ◽  
Author(s):  
J. Jiménez ◽  
M. E. Cisneros-Ortiz ◽  
Y. Guardia-Puebla ◽  
J. M. Morgan-Sagastume ◽  
A. Noyola
Keyword(s):  
Rice Straw ◽  
Methane Production ◽  
Hydrolytic Activity ◽  
Interactive Effects ◽  
Pig Manure ◽  
Agriculture Waste ◽  
Methanogenic Activity ◽  
Thermophilic Conditions ◽  
Specific Methanogenic Activity ◽  
Positive Effect

The anaerobic co-digestion of three wastes (manure, rice straw and clay residue, an inorganic additive) at different concentration levels and their interactive effects on methanogenic activity were investigated in this work at thermophilic conditions in order to enhance hydrolytic activity and methane production. A central composite design and the response surface methodology were applied for the optimization of specific methanogenic activity (SMA) by assessing their interaction effects with a reduced number of experiments. The results showed a significant interaction among the wastes on the SMA and confirmed that co-digestion enhances methane production. Rice straw apparently did not supply a significant amount of substrate to make a difference in SMA or methane yield. On the other hand, clay residue had a positive effect as an inorganic additive for stimulating the anaerobic process, based on its mineral content and its adsorbent properties for ammonia. Finally, the optimal conditions for achieving a thermophilic SMA value close to 1.4 g CH4-COD/g VSS · d−1 were 20.3 gVSS/L of manure, 9.8 gVSS/L of rice straw and 3.3 gTSS/L of clay.

The effect of operational conditions on the sludge specific methanogenic activity and sludge biodegradability

2009 ◽  
Vol 59 (9) ◽  
pp. 1847-1853 ◽  
Author(s):  
R. C. Leitão ◽  
S. T. Santaellla ◽  
A. C. van Haandel ◽  
G. Zeeman ◽  
G. Lettinga
Keyword(s):  
Municipal Wastewater ◽  
Pilot Scale ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Treatment Unit ◽  
Methanogenic Activity ◽  
Operational Conditions ◽  
Anaerobic Reactors ◽  
Specific Methanogenic Activity

The effects of hydraulic retention time (HRT) and influent COD concentration (CODInf) on Specific Methanogenic Activity (SMA) and the biodegradability of an anaerobic sludge need to be elucidated because of the discordant results available in literature. This information is important for the operation of anaerobic reactors and design of the sludge post-treatment unit. For this study, sludge samples obtained from eight pilot-scale Upflow Anaerobic Sludge Blanket (UASB) reactors were tested. The reactors were fed with municipal wastewater and operated with different sets of HRT and influent concentrations until the steady state was established. The results show that at a lower HRT, sludge with relatively higher SMA develops. A slight trend of declining SMA at increasing CODInf was found for reactors operated at longer HRTs; however, further experiments are necessary for more definitive conclusions. The sludge from reactors operated at longer HRTs and with lower CODInf resulted in lower biodegradability. Results also showed that it is ineffective to design a UASB reactor with a longer HRT to cope with organic shock loads.

Influence of moisture content on the specific methanogenic activity of dry mesophilic municipal solid waste digestate

2011 ◽  
Vol 87 (7) ◽  
pp. 1032-1035 ◽  
Author(s):  
Ronan Le Hyaric ◽  
Hassen Benbelkacem ◽  
Julien Bollon ◽  
Rémy Bayard ◽  
Renaud Escudié ◽  
...  
Keyword(s):  
Moisture Content ◽  
Municipal Solid Waste ◽  
Solid Waste ◽  
Methanogenic Activity ◽  
Specific Methanogenic Activity
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