Optimization of the thermophilic anaerobic co-digestion of pig manure, agriculture waste and inorganic additive through specific methanogenic activity

2014 ◽  
Vol 69 (12) ◽  
pp. 2381-2388 ◽  
Author(s):  
J. Jiménez ◽  
M. E. Cisneros-Ortiz ◽  
Y. Guardia-Puebla ◽  
J. M. Morgan-Sagastume ◽  
A. Noyola
Keyword(s):  
Rice Straw ◽  
Methane Production ◽  
Hydrolytic Activity ◽  
Interactive Effects ◽  
Pig Manure ◽  
Agriculture Waste ◽  
Methanogenic Activity ◽  
Thermophilic Conditions ◽  
Specific Methanogenic Activity ◽  
Positive Effect

The anaerobic co-digestion of three wastes (manure, rice straw and clay residue, an inorganic additive) at different concentration levels and their interactive effects on methanogenic activity were investigated in this work at thermophilic conditions in order to enhance hydrolytic activity and methane production. A central composite design and the response surface methodology were applied for the optimization of specific methanogenic activity (SMA) by assessing their interaction effects with a reduced number of experiments. The results showed a significant interaction among the wastes on the SMA and confirmed that co-digestion enhances methane production. Rice straw apparently did not supply a significant amount of substrate to make a difference in SMA or methane yield. On the other hand, clay residue had a positive effect as an inorganic additive for stimulating the anaerobic process, based on its mineral content and its adsorbent properties for ammonia. Finally, the optimal conditions for achieving a thermophilic SMA value close to 1.4 g CH4-COD/g VSS · d−1 were 20.3 gVSS/L of manure, 9.8 gVSS/L of rice straw and 3.3 gTSS/L of clay.

Optimization of the specific methanogenic activity during the anaerobic co-digestion of pig manure and rice straw, using industrial clay residues as inorganic additive

2015 ◽  
Vol 259 ◽  
pp. 703-714 ◽  
Author(s):  
J. Jiménez ◽  
Y. Guardia-Puebla ◽  
M.E. Cisneros-Ortiz ◽  
J.M. Morgan-Sagastume ◽  
G. Guerra ◽  
...  
Keyword(s):  
Rice Straw ◽  
Pig Manure ◽  
Methanogenic Activity ◽  
Specific Methanogenic Activity

scholarly journals Enhancement of Methane Production in Thermophilic Anaerobic Co-Digestion of Exhausted Sugar Beet Pulp and Pig Manure

2019 ◽  
Vol 9 (9) ◽  
pp. 1791 ◽  
Author(s):  
Xiomara Gómez-Quiroga ◽  
Kaoutar Aboudi ◽  
Carlos José Álvarez-Gallego ◽  
Luis Isidoro Romero-García
Keyword(s):  
Sugar Beet ◽  
Methane Production ◽  
Volatile Fatty Acids ◽  
Biogas Production ◽  
Methanogenic Archaea ◽  
Sugar Beet Pulp ◽  
Pig Manure ◽  
Thermophilic Conditions ◽  
Beet Pulp ◽  
The Individual

In this paper, the viability of thermophilic anaerobic co-digestion of exhausted sugar beet pulp (ESBP) and pig manure (PM) was evaluated. The effect of the proportion of ESBP on biogas production was investigated by using a series of lab-scale batch assays, in duplicates. The following five ESBP:PM mixture ratios were studied: 0:100, 10:90, 25:75, 50:50, and 100:0. The highest cumulative methane production (212.4 mL CH4/g VSadded) was reached for the mixture 25:75. The experimental results showed that the increase in the proportion of ESBP in the mixture led to the distortion of the process, due to acidification by the volatile fatty acids generated. Acetic acid was the predominant acid in all the cases, representing more than 78% of the total acidity. Moreover, the results obtained by operating at thermophilic temperatures have been compared with those obtained in a previous study conducted at mesophilic temperatures. The results have shown that in the individual digestion of ESBP, the activity of acetoclastic methanogens was affected in both temperatures, but especially in thermophilic conditions. Thus, the methane produced in the individual thermophilic digestion of ESBP came almost entirely from the activity of hydrogen-utilizing methanogenic archaea.

Anaerobic Treatment of Synthetic Sulfate-Containing Wastewater under Thermophilic Conditions

1992 ◽  
Vol 25 (7) ◽  
pp. 193-202 ◽  
Author(s):  
A. Visser ◽  
Y. Gao ◽  
G. Lettinga
Keyword(s):  
Waste Water ◽  
Steady State ◽  
Methane Production ◽  
Anaerobic Treatment ◽  
Uasb Reactor ◽  
Methanogenic Activity ◽  
Sulfate Reducers ◽  
Activity Measurements ◽  
Thermophilic Conditions ◽  
Temperature Optima

The anaerobic treatment of a sulfate-containing waste water using a UASB reactor was studied at 55 °C. As substrate, acetate and a mixture of acetate, propionate and butyrate were used. With acetate as substrate it was shown that sulfate reducers are capable of using acetate as substrate at 55 °C, and that, under the conditions applied, they even outcompete acetoclastic methanogens. Batch-activity measurements with the sludge revealed temperature optima for acetate, propionate and butyrate degradation of ± 56-59, < 40 and 52-54 °C respectively. After switching the substrate to a mixture of acetate, propionate and butyrate, the reactor pH dropped from 8.3-8.6 to 7.6-7.9 and the methane production recovered. After the establishment of a pseudo-steady state situation the part of COD removed by methane production and sulfate reduction was ± 60 and 40 %. Results of batch activity experiments showed that the methanogenic activity dropped sharply at pH ≥ 8 and ≤ 6 causing a predominance of sulfate reducers at pH ≥ 8.

scholarly journals Anaerobic Co-digestion of Rice Straw and Pig Manure Pretreated With a Cellulolytic Microflora: Methane Yield Evaluation and Kinetics Analysis

2021 ◽  
Vol 8 ◽  
Author(s):  
Bin Zhong ◽  
Xuejiao An ◽  
Fei Shen ◽  
Weijuan An ◽  
Qinghua Zhang
Keyword(s):  
Rice Straw ◽  
Methane Production ◽  
Methane Yield ◽  
Energy Output ◽  
Organic Loading Rate ◽  
Pig Manure ◽  
Control Group ◽  
Lag Phase ◽  
Net Energy ◽  
Order Kinetic

Agricultural wastes, such as rice straw (RS) and pig manure (PM), cause serious environmental pollution due to the non-existence of effective disposal methods. Urgent investigations are needed to explore how such wastes can be transformed into resources. In this study, we comprehensively assessed methane yield and kinetics of RS and PM anaerobic co-digestion, with or without pretreatment of a previously developed cellulolytic microflora, under conditions of their maximum organic loading rate. The anaerobic co-digestion results revealed that the cumulative methane production of RS and PM after bio-pretreatment was 342.35 ml (g-VS)−1, which is 45% higher than that of the control group [236.03 ml·(g-VS)−1]. Moreover, the kinetic analysis showed the first-order kinetic, while the modified Gompertz models revealed higher fitting properties (R2 ≥ 0.966). After bio-pretreatment, the hydrolytic constant, maximum accumulative methane production, and maximum methane production rates of RS and PM reached 0.46 day−1, 350.79 ml·(g-VS)−1, and 45.36 ml·(g-VS)−1·day−1, respectively, which were 77, 45.1, and 84.3% higher than those without pretreatment. Also, we found that the lag phase and effective methane production time after bio-pretreatment decreased from 2.43 to 1.79 days and 10.7 to 8.92 days, respectively. Upon energy balance evaluation, we reported a net energy output of 5133.02 kWh·ton−1 after bio-pretreatment. Findings from this present study demonstrated that bio-pretreatment of RS and PM mixtures with cellulolytic microflora could greatly enhance methane production and anaerobic digestion efficiency.

scholarly journals Progressive Degradation of Crude Oil n-Alkanes Coupled to Methane Production under Mesophilic and Thermophilic Conditions

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e113253 ◽  
Author(s):  
Lei Cheng ◽  
Shengbao Shi ◽  
Qiang Li ◽  
Jianfa Chen ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Crude Oil ◽  
Methane Production ◽  
Thermophilic Conditions

Sludge accumulation and methanogenic activity in an anaerobic lagoon

2000 ◽  
Vol 42 (10-11) ◽  
pp. 247-255 ◽  
Author(s):  
J. Paing ◽  
B. Picot ◽  
J. P. Sambuco ◽  
A. Rambaud
Keyword(s):  
Anaerobic Digestion ◽  
Methane Production ◽  
Anaerobic Degradation ◽  
Volatile Fatty Acids ◽  
Anaerobic Fermentation ◽  
Methanogenic Activity ◽  
Start Up ◽  
Anaerobic Lagoon ◽  
Methane Production Rate ◽  
Sludge Accumulation

Sludge accumulation and the characteristics of anaerobic digestion in sludge had been investigated in a primary anaerobic lagoon. Methanogenic potential of sludge was evaluated by an anaerobic digestion test which measured the methane production rate. Sludge was sampled at several points in the lagoon to determine spatial variations and with a monthly frequency from the start-up of the lagoon to observe the development of anaerobic degradation. Maximum amounts of sludge accumulated near the inlet. The mean methane production of sludge was 2.9 ml gVS–1 d–1. Sludge near the outlet presented a greater methanogenic activity and a lesser concentration of volatile fatty acids than near the inlet. The different stages of anaerobic degradation were spatially separated, acidogenesis near the inlet and methanogenesis near the outlet. This staged distribution seemed to increase efficiency of anaerobic fermentation compared with septic tanks. Methane release at the surface of the lagoon was estimated to be very heterogeneous with a mean of 25 l m–2 d–1. The development of performance and sludge characteristics showed the rapid beginning of methanogenesis, three months after the start-up of the anaerobic lagoon. Considering the volume of accumulated sludge, it could however be expected that methanogenic activity would further increase.

Analysis of environmental factors affecting methane production from high-solids organic waste

1997 ◽  
Vol 36 (6-7) ◽  
pp. 493-500 ◽  
Author(s):  
J. J. Lay ◽  
Y. Y. Li ◽  
T. Noike ◽  
J. Endo ◽  
S. Ishimoto
Keyword(s):  
Moisture Content ◽  
Methane Production ◽  
Organic Waste ◽  
High Solids ◽  
Methanogenic Activity ◽  
Factors Affecting ◽  
Bacterial System ◽  
Sludge Cake ◽  
Production Curve ◽  
Ph Range

A simple model developed from the Gompertz equation was used to describe the cumulative methane production curve in the batch culture. By using this model, three key parameters, namely methane production rate, potential and lagphase time, in a cumulative methane production curve were exactly estimated based on the experimental data. The results indicate that each gram of dry organic waste of a sludge cake, meat, carrot, rice, potato and cabbage had a methane production potential of 450, 424, 269, 214, 203 and 96 mL, respectively. The methanogenic activity of these digesters decreased with a decrease in the moisture content. The moisture content threshold limit, at which the methanogenic activity dropped to zero, was found to be 56.6% for the sludge cake, but greater than 80% for meat, carrot and cabbage. In the high-solids sludge digestion, the relative methanogenic activity dropped from 100% to 53% when the moisture content decreased from 96% to 90%. The rate of methane production at moisture contents of 90% to 96% functioned in a pH range between 6.6 and 7.8, but optimally at pH 6.8, and the process may fail if the pH was lower than 6.1 or higher than 8.3. On the other hand, the methanogenic activity was dependent on the level of ammonium, NH4+, but not free ammonia, NH3, indicating that the NH4+ was the more significant factor rather than the NH3 in affecting the methanogenic activity of a well-acclimatized bacterial system. In the wide pH range of 6.5 to 8.5, the methanogenic activity decreased with the increase in the NH4+; dropped 10% at the NH4+-N concentration of 1670-3720 mg·L−1, 50% at 4090-5550 mg·L−1 and dropped to zero at 5880-6600 mg·L−1. However, the lagphase time was dependent on the NH3 level, but not on NH4+, and when NH3-N was higher than 500 mg·L−1, a notable shock was observed. This suggests that the NH3 level was the more sensitive factor than the NH4+ level for an unacclimatized bacterial system.

scholarly journals Examination and optimization of lignocellulolytic activity of S. gausapatum F28 on beechwood sawdust supplemented with molasses stillage

2021 ◽  
pp. 75-75
Author(s):  
Jelena Jovic ◽  
Jian Hao ◽  
Ljiljana Mojovic
Keyword(s):  
Manganese Peroxidase ◽  
Incubation Temperature ◽  
Hydrolytic Activity ◽  
Optimization Techniques ◽  
Cultivation Conditions ◽  
Beet Molasses ◽  
Response Optimization ◽  
Substrate Moisture ◽  
Lignocellulolytic Activity ◽  
Positive Effect

This study provides detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses stillage. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g-1 substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g-1 substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5?C, and pH 5.2. The research showed that the addition of molasses stillage had positive effect on the enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was <5 %, manganese peroxidase ~12 %, cellulase ~21 %, xylanase ~16 %), which should be taken into consideration when optimizing the desired process.

scholarly journals MATHEMATICAL MODELS FOR ESTIMATING METHANE PRODUCTION IN COVERED LAGOON BIODIGESTERS TREATING PIG MANURE

2021 ◽  
Vol 41 (4) ◽  
pp. 438-448
Author(s):  
Juciara O. Lopes ◽  
André P. Rosa ◽  
Izabelle de P. Sousa ◽  
Nathalia S. Oliveira ◽  
Alisson C. Borges
Keyword(s):  
Mathematical Models ◽  
Methane Production ◽  
Pig Manure
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