scholarly journals Detection of enteric viruses in Hungarian surface waters: first steps towards environmental surveillance

2013 ◽  
Vol 11 (4) ◽  
pp. 772-782 ◽  
Author(s):  
Anita Kern ◽  
Mihaly Kadar ◽  
Katalin Szomor ◽  
György Berencsi ◽  
Beatrix Kapusinszky ◽  
...  

Waterborne viruses infect the human population through the consumption of contaminated drinking water and by direct contact with polluted surface water during recreational activity. Although water related viral outbreaks are a major public health concern, virus detection is not a part of the water quality monitoring scheme, mainly due to the absence of routine analysis methods. In the present study, we implemented various approaches for water concentration and virus detection, and tested on Hungarian surface water samples. Eighty samples were collected from 16 sites in Hungary. Samples were concentrated by glass wool and membrane filtration. Human adenoviruses were detected by conventional and quantitative real-time polymerase chain reaction (PCR) methods in 56% (45/80) of the samples; viral titers ranged from 8.60 × 101 to 3.91 × 104 genome copies per liter. Noroviruses and enteroviruses were detected in 30% (24/80) and 13% (10/80) of samples, respectively, by reverse transcription-PCR assays. Results indicate a high prevalence of viral human pathogens in surface waters, suggesting the necessity of a detailed survey focusing on the quality of natural bathing waters and drinking water sources.

1993 ◽  
Vol 27 (7-8) ◽  
pp. 127-133 ◽  
Author(s):  
H. Dizer ◽  
J. Dürkop ◽  
A. Grohmann ◽  
H. Kopecka ◽  
J. M. López-Pila

Secondary effluent of wastewater treatment plants contains a high number of viruses and other pathogens, which pose a health risk to the population, (especially when receiv ng waters are used for bathing and swimming, or for growing shellfish. In areas with a high density of population, where drinking water supply is dependent on surface waters and contaminated rivers are the primary source of drinking water, failure of the filtration or of the disinfection step, or of any other “barriers” supposed to warrant safe potable water, will increase the risk of health hazard for the consumer. We have compared the efficiency of viral elimination in secondary effluent by flocculation, uv rradiation and membrane filtration taking naturally occurring, or additionally seeded f2 phages, as indicator for viruses. Flocculation decreased the number of phages present in secondary effluent by more than two logs. If combined with uv irradiation, the elimination reached five additional logs. Membrane filtration eliminated essentially all naturally occurring phages. Improvement of the quality of surface waters calls for a refinement of detection methods for viruses. We have found that the polymerase chain reaction (PCR) might be used for detecting viruses in surface waters.


2012 ◽  
Vol 84 (2) ◽  
pp. 427-442 ◽  
Author(s):  
Winston F.O. Gonçalves ◽  
Wanilson Luiz-Silva ◽  
Wilson Machado ◽  
Erico C. Nizoli ◽  
Ricardo E. Santelli

The geochemical composition of sediment pore water was investigated in comparison with the composition of sediment particles and surface water in an estuary within one of the most industrialized areas in Latin America (Santos-Cubatão estuarine system, SE Brazil). Pore and surface waters presented anomalously high levels of F-, NH4+, Fe, Mn and P due to two industrial point sources. In the summer, when SO4(2-)/Cl- ratios suggested an enhanced sulfate reduction, the higher dissolved levels observed in pore waters for some metals (e.g., Cu and Ni) were attributed to reductive dissolution of oxidized phases. Results evidenced that the risks of surface water concentration increase due to diffusion or advection from pore water are probably dependent on coupled influences of tidal pumping and groundwater inputs.


2007 ◽  
Vol 6 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Maria Tereza Pepe Razzolini ◽  
Marisa Di Bari ◽  
Petra Sanchez Sanchez ◽  
Maria Inês Zanoli Sato

Aeromonads are inhabitants of aquatic ecosystems and are described as being involved in intestinal disturbances and other infections. A total of 200 drinking water samples from domestic and public reservoirs and drinking fountains located in São Paulo (Brazil), were analyzed for the presence of Aeromonas. Samples were concentrated by membrane filtration and enriched in APW. ADA medium was used for Aeromonas isolation and colonies were confirmed by biochemical characterization. Strains isolated were tested for hemolysin and toxin production. Aeromonas was detected in 12 samples (6.0%). Aeromonas strains (96) were isolated and identified as: A. caviae (41.7%), A.hydrophila (15.7%), A.allosacharophila (10.4%), A. schubertii (1.0%) and Aeromonas spp. (31.2%).The results revealed that 70% of A. caviae, 66.7% of A. hydrophila, 80% of A. allosacharophila and 46.6% of Aeromonas spp. were hemolytic. The assay for checking production of toxins showed that 17.5% of A. caviae, 73.3% of A. hydrophila, 60% of A. allosacharophila, 100% of A. schubertii, and 33.3% of Aeromonas spp. were able to produce toxins. The results demonstrated the pathogenic potential of Aeromonas, indicating that the presence of this emerging pathogen in water systems is a public health concern.


2020 ◽  
Vol 5 (2) ◽  
pp. 138-147
Author(s):  
S. L. Afegbua ◽  

The Sustainable Development Goal on sanitation aims to achieve universal access to good health, affordable drinking water, sanitation and an end to open defeacation by 2030. The recent ranking of Nigeria as first globally for open defecation is of public and environmental health concern. This study assessed the sanitary condition and the microbiological quality of well and surface waters of Panhauya community and Ahmadu Bello University farm, Zaria, and the antibiogram of the bacterial isolates.. Based on the WHO criteria, the sanitary inspection showed that 16.7%, 54.2%, 25% and 4.2% of the water sampling points had a very high, high, intermediate and low risk of contamination respectively. Occurrence of Escherichia coli, Giardia lamblia, Entamoeba histolytica, Pseudomonas aeruginosa, Salmonella spp and Vibrio cholerae in water samples from Panhuaya community was 87.5%, 75%, 68.8%, 50%, 25% and 12.5% respectively. In ABU farm Shika, the occurrence was; E. coli (75%), E. histolytica (63%), G. lamblia and Salmonella spp. All E. coli isolates exhibited high multidrug resistance to antibiotics screened with a MAR index of 0.3-0.8. The drinking water sources in Panhuaya and ABU farm were unsafe and the presence of these pathogens in the water samples may be attributed to a number of factors including poor sanitation, manure application and open defecation practice. This indicates a public health risk to the residents and emphasises the need for safe water supplies sanitation and antibiotic stewardship. Keywords: Well water; surface water; sanitary inspection; open defecation; water-borne pathogens; Zaria.


1991 ◽  
Vol 24 (2) ◽  
pp. 35-42 ◽  
Author(s):  
R. Helmer ◽  
I. Hespanhol ◽  
L. J. Saliba

Over the past Decade, the World Health Organization has developed a series of microbiological guidelines covering different aspects of the aquatic environment. Transmission pathways of human pathogens from their faecal origin back to man via drinking-water, bathing waters, shellfish and sewage-irrigated crops were investigated and epidemiology-based health criteria established. The total and faecal coliform guideline values for drinking-water are generally complied with in urban water supplies but difficult to meet in rural areas of developing countries. The Mediterranean serves as the major study area for assessing health risks from bathing in coastal waters and from the consumption of shellfish eaten uncooked. Wastewater reuse for crop irrigation is rapidly expanding due to water shortages but poses health risks to farm workers and consumers. New guidelines for control of helminths were recently issued by a WHO Scientific Group. A comparative evaluation of the above guidelines follows.


MRS Advances ◽  
2018 ◽  
Vol 3 (36) ◽  
pp. 2089-2098 ◽  
Author(s):  
Agatha W. Wagutu ◽  
Revocatus L. Machunda ◽  
Yusufu Abeid Chande Jande

ABSTRACTIn Northern Tanzania, high levels of fluoride in community drinking water supply is recognized as one of the major public health concern, the problem is further ameliorated by presence Escherichia coli and fecal coliform bacteria in surface water and shallow wells. Efforts to decontaminate the water involve mostly the use of low efficient bone char for fluoride removal without disinfecting the pathogens. To address this problem, a robust adsorbent which is capable of removing fluoride and microbes simultaneously with minimal diverse impact on the treated water is necessary. Here we highlight development of composite material developed from recycling of crustacean biomass waste from sea food industry. Chitosan polymer, isolated from prawns shell was composited with crab shell derived brushite (CaHPO4.2H2O) to form chitosan-hydroxyapatite composite. XRD and FT-IR analysis confirmed transformation of brushite phases into hydroxyapatite and formation hybrid composite. Fluoride adsorption tests were performed in batch mode to evaluate effectiveness. Defluoridation capacity of up to 6.4 mg/g in field water containing fluoride concentration of 5-70 mg/L was achieved. The best performance was observed with fluoride concentration of 10 mg/L and below. Apart from fluoride removal, the composite also reduced color tint and microbes from surface water samples. The pH of the treated water in most samples remained around 6.5-8.5, which is acceptable for drinking water.


2008 ◽  
Vol 57 (11) ◽  
pp. 1693-1698 ◽  
Author(s):  
C. T. Menezes ◽  
L. Heller

The occurrence of pesticides in drinking water is a matter of growing concern in several parts of the world, mainly in developing countries, due the possible adverse effects on human health. Pesticides applied in the agriculture are an important source of contamination and are rarely monitored in surface water in developing countries, either by water supply operators or health authorities, often not accomplishing the legal issues regarding the quality control of raw waters. The paper discusses a method for prioritization of surveillance actions of pesticides in surface waters, through multicriteria analysis. Five criteria were defined and a range of weight was established for each criterion. For validation of the method, it was applied in five sub-basins of Grande River Basin—MG. This application allowed ordering priority sub-basins for pesticides surveillance, suggesting two sub-basins as priorities. The validation performed enabled the evaluation and adjustment of the method, mainly regarding the availability of information. The method showed a practical alternative for the environmental surveillance, targeting priority areas. Moreover, its structure allows the application in other different areas and for other pollutants.


Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1603
Author(s):  
Emily S. Bailey ◽  
Margret Hopkins ◽  
Lisa Casanova ◽  
Mark D. Sobsey

Surface waters used for drinking water supply often receive upstream wastewater effluent inputs, resulting in de facto wastewater reuse for drinking water and recreation. As populations grow, demands on water supplies increase. As this trend continues, it creates the need to understand the risks associated with such reuse. In North Carolina, potable reuse has been proposed as a combination of at least 80% surface water with up to 20% tertiary-treated, dual-disinfected, reclaimed wastewater, which is then stored for 5 days and further treated using conventional drinking water treatment methods. The state of North Carolina has set standards for both intake surface water and for the reclaimed water produced by wastewater utilities, using indicator microorganisms to measure compliance. The goal of this study was to quantify fecal indicator microorganisms, specifically E. coli, coliphages, and C. perfringens as well as key pathogens, specifically Salmonella spp. bacteria, adenoviruses, noroviruses, and the protozoan parasites Cryptosporidium and Giardia, in two types of water representing potential candidates for potable reuse in North Carolina, (1) run of river surface water and (2) sewage-impacted surface waters, with the purpose of determining if there are predictive relationships between these two microorganism groups that support microbial indicator reliability.


Author(s):  

The article is devoted to the analysis of the quality of surface water in Uzbekistan. Analyzed their impact on the living conditions and health of the population of the republic. The most difficult situation with drinking water supply and with the growth of diseases caused by the use of poor-quality drinking water falls on the Navoi, Khorezm, Surkhandarya regions and Karakalpakstan. The stated data makes it relevant to conduct research to determine the quality of surface waters and improve the methods of its assessment. The analysis of existing methods for assessing the quality of surface waters. The technique of assessment of the index of pollution of water applied now in Uzbekistan counted in compliance with “Methodical recommendations about the formalized complex evaluation test of the surface and ocean waters about hydro/chemical indexes” was developed in Soviet period and doesn’t meet the modern requirements. The need to improve the methods for assessing the quality of surface waters as compared with that currently used in Uzhydromet is indicated. One of the most promising at present is the “Method of integrated assessment of the degree of contamination of surface waters by hydro/chemical indicators” developed and used in operational practice in Russia. The most informative comprehensive estimat water pollution and the class of water quality. The author proposed methods for assessing the quality of surface waters, the calculation of which is based on the use of a larger amount of initial information and are more reliable than those used. In particular, the proposed methodology for assessing the quality of surface waters, taking into account the hazard class of ingredients.


2020 ◽  
Author(s):  
Alexander Ahring ◽  
Marvin Kothe ◽  
Christian Gattke ◽  
Ekkehard Christoffels ◽  
Bernd Diekkrüger

<p>Inland surface waters like rivers, streams, lakes and reservoirs are subject to anthropogenic pollutant emissions from various sources. These emissions can have severe negative impacts on surface water ecology, as well as human health when surface waters are used for recreational activities, irrigation of cropland or drinking water production. In order to protect aquatic ecosystems and freshwater resources, the European Water Framework Directive (WFD) sets specific quality requirements which the EU member states must meet until 2027 for every water body.</p><p>Implementing effective measures and emission control strategies requires knowledge about the important emission pathways in a given river basin. However, due to the abundance of pollution sources and the heterogeneity of emission pathways in time and space, it is not feasible to gain this knowledge via water quality monitoring alone. In our study, we aim to combine SWAT ecohydrological modelling and long term water quality monitoring data to establish a spatially differentiated nitrogen emission inventory on the sub-catchment scale. SWAT (short for Soil and Water Assessment Tool) is a semi-distributed, dynamic and process-driven watershed model capable of simulating long term hydrology as well as nutrient fluxes on a daily time step.</p><p>The study area is the Swist river basin in North Rhine-Westphalia (Germany). Belonging to the Rhine river system, the Swist is the largest tributary of the Erft River and drains a basin area of approximately 290 km². As part of its legal obligations and research activities, the Erftverband local waterboard collects a large variety of long term monitoring data in the Swist river catchment, which is available for this study. This includes operational data from the wastewater treatment plants in the watershed, discharge data from four stream gauging stations, river water quality data from continuous and discontinuous monitoring, groundwater quality data as well as quality data from surface, sub-surface and tile drainage runoff from various land uses.</p><p>Our contribution will be made up of two equal parts: First, we will present our water quality monitoring activities in the catchment and the related data pool outlined above, with special emphasis on recent monitoring results from agricultural tile drainages. Apart from nutrients and other pollutants, the data suggests considerable inputs of herbicide transformation products like Chloridazon-Desphenyl (maximum concentration measured: 15 µg/l) via this pathway. Second, we will explain how we integrate the monitoring data into the SWAT simulations and how we tackle related challenges like parameter equifinality (meaning that multiple parameter sets can yield similar or identical model outputs). The overall goal is to take all possible emission pathways into consideration, including those often neglected in past SWAT studies, like tile drainages and combined sewer overflows (CSO). As the Swist catchment is affected by groundwater extraction due to lignite mining in the Lower Rhine Bay area, we will discuss how this is considered during SWAT model setup and calibration, and will present first simulation results concerning catchment hydrology.</p>


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