Bioassays and biomarkers for ecotoxicological assessment of reclaimed municipal wastewater

2008 ◽  
Vol 57 (6) ◽  
pp. 947-953 ◽  
Author(s):  
A. Kontana ◽  
M. Yiangou ◽  
C. A. Papadimitriou ◽  
P. Samaras ◽  
A. Zdragas
Keyword(s):  
Municipal Wastewater ◽  
Vibrio Fischeri ◽  
Tertiary Treatment ◽  
Reclaimed Wastewater ◽  
Immunological Parameters ◽  
Test Species ◽  
Mouse Splenocytes ◽  
Microbiological Characteristics ◽  
Cytokine Levels ◽  
Wastewater Samples

The aim of this work was to examine the ecotoxicity of reclaimed wastewater by the use of bioassays and the determination of immunological parameters. Secondary and tertiary mucicipal wastewater samples were examined for their physicochemical and microbiological characteristics as well as for their endotoxin concentrations. The ecotoxicological characteristics were assessed by a battery of bioassays, using Vibrio fischeri, Daphnia magna and Tetrahymena thermophilla as test species and phytotoxicity. The mitogenic responses of mouse splenocytes were as well used as bioassay. The cytokines of IL-1, IL-2, IL-10, IFNγ and TNFα, were also determined in the supernatant of splenocyte cultures and served as molecular biomarkers. All bioassays exhibited decrease of the ecotoxicological responses after tertiary treatment. However, mitogenic responses were proved to be more sensitive. IL-1 increased, while IL-2 production was unaffected. The fact that IL-10 production increased in response to secondary treated effluents in conjunction with the increased endotoxin levels, suggest Th2 type immune responses. Although results obtained from the toxicity bioassays after the tertiary treatment showed comparable results to those of controls, cytokine levels indicated the induction of immune response even after tertiary treatment. Consequently, cytokine production could be used as a sensitive biomarker for the evaluation of treatment efficiency of the reclaimed wastewaters intended for reuse.

Effectiveness of ozonation and chlorination on municipal wastewater treatment evaluated by a battery of bioassays and biomarkers

2009 ◽  
Vol 60 (6) ◽  
pp. 1497-1505 ◽  
Author(s):  
A. Kontana ◽  
C. A. Papadimitriou ◽  
P. Samaras ◽  
A. Zdragas ◽  
M. Yiangou
Keyword(s):  
Activated Carbon ◽  
Municipal Wastewater ◽  
Interleukin 1 ◽  
Granular Activated Carbon ◽  
Municipal Wastewater Treatment ◽  
Tertiary Treatment ◽  
Reclaimed Wastewater ◽  
Test Species ◽  
Mouse Splenocytes ◽  
Battery Of Bioassays

A battery of bioassays, including biological toxicity as well as in vitro mouse spleen lymphoproliferative responses and cytokine production, was conducted to compare the effectiveness of tertiary treatment methods such as coagulation (Coag) and absorption on granular activated carbon (GAC) and disinfection processes such as chlorination and ozonation in removing toxic or stress inducing agents from reclaimed wastewater. Whole effluent toxicity (WET) testing of secondary treated (ST) wastewater using as test species Vibrio fischeri, Daphnia magna and Tetrahymena thermophila as well as phytotoxicity revealed moderate toxicity effects that depend on the organism used. All bioassays exhibited decrease of the ecotoxicological responses after tertiary treatment. However, mitogenic responses were proved to be more sensitive. Endotoxin present in ST samples may be responsible for the increased strong lymphoproliferative activity as well as interleukin-1 (IL-1) production by mouse splenocytes. Tertiary treatment of ST with coagulation and/or adsorption on granular activated carbon (GAC) in combination with ozonation reduced WET to control levels. Ozonation alone or in combination with any other treatment removed endotoxin more efficiently than chlorination and thus reduced spleen lymphoproliferative responses and IL-1 production.

scholarly journals Assessment of Microplastics in a Municipal Wastewater Treatment Plant with Tertiary Treatment: Removal Efficiencies and Loading per Day into the Environment

Water ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1339
Author(s):  
Javier Bayo ◽  
Sonia Olmos ◽  
Joaquín López-Castellanos
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Municipal Wastewater ◽  
Removal Rate ◽  
Treatment Plant ◽  
Source Control ◽  
Municipal Wastewater Treatment ◽  
Tertiary Treatment ◽  
Stable Performance ◽  
Wastewater Samples

This study investigates the removal of microplastics from wastewater in an urban wastewater treatment plant located in Southeast Spain, including an oxidation ditch, rapid sand filtration, and ultraviolet disinfection. A total of 146.73 L of wastewater samples from influent and effluent were processed, following a density separation methodology, visual classification under a stereomicroscope, and FTIR analysis for polymer identification. Microplastics proved to be 72.41% of total microparticles collected, with a global removal rate of 64.26% after the tertiary treatment and within the average retention for European WWTPs. Three different shapes were identified: i.e., microfiber (79.65%), film (11.26%), and fragment (9.09%), without the identification of microbeads despite the proximity to a plastic compounding factory. Fibers were less efficiently removed (56.16%) than particulate microplastics (90.03%), suggesting that tertiary treatments clearly discriminate between forms, and reporting a daily emission of 1.6 × 107 microplastics to the environment. Year variability in microplastic burden was cushioned at the effluent, reporting a stable performance of the sewage plant. Eight different polymer families were identified, LDPE film being the most abundant form, with 10 different colors and sizes mainly between 1–2 mm. Future efforts should be dedicated to source control, plastic waste management, improvement of legislation, and specific microplastic-targeted treatment units, especially for microfiber removal.

scholarly journals Assessment of Microplastics in a Municipal Wastewater Treatment Plant With Tertiary Treatment: Removal Efficiencies and Loading Per Day Into the Environment

2021 ◽  
Author(s):  
Javier Bayo ◽  
Sonia Olmos ◽  
Joaquín López-Castellanos
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Municipal Wastewater ◽  
Removal Rate ◽  
Treatment Plant ◽  
Source Control ◽  
Municipal Wastewater Treatment ◽  
Tertiary Treatment ◽  
Stable Performance ◽  
Wastewater Samples

Abstract This study investigates the removal of microplastics from wastewater in an urban wastewater treatment plant located in Southeast Spain, including an oxidation ditch, rapid sand filtration, and ultraviolet disinfection. A total of 146.73 L of wastewater samples from influent and effluent were processed, following a density separation methodology, visual classification under a stereomicroscope, and FTIR analysis for polymer identification. Microplastics proved to be 72.41% of total microparticles collected, with a global removal rate of 64.26% after the tertiary treatment and within the average retention for European WWTPs. Three different shapes were identified: i.e., microfiber (79.65%), film (11.26%), and fragment (9.09%), without the identification of microbeads despite the proximity to a plastic compounding factory. Fibers were less efficiently removed (56.16%) than particulate microplastics (90.03%), suggesting that tertiary treatments clearly discriminate among forms, and reporting a daily emission of 1.6 x 107 microplastics to the environment. Year variability in microplastic burden was cushioned at the effluent, reporting a stable performance of the sewage plant. Eight different polymer families were identified, LDPE film being the most abundant form. Future efforts should be carried on source control, plastic waste management, improvement of legislation, and specific microplastic-targeted treatment units, especially for microfiber removal.

Reclaimed municipal wastewater as source of water and nutrients for plant nurseries

2004 ◽  
Vol 50 (2) ◽  
pp. 69-75 ◽  
Author(s):  
R. Gori ◽  
C. Lubello ◽  
F. Ferrini ◽  
F. Nicese
Keyword(s):  
Municipal Wastewater ◽  
Central Italy ◽  
Growing Season ◽  
Dry Mass ◽  
Well Water ◽  
Tertiary Treatment ◽  
Reclaimed Wastewater ◽  
Experimental Activity ◽  
Treatment Facilities ◽  
Italian Legislation

A three year experimental activity was carried out to evaluate the possibility of reusing Reclaimed Wastewater (RWW) in Pistoia's nursery area (Central Italy). The research was aimed at: 1. identifying the best tertiary treatment facilities in order to make the effluent in compliance with the current Italian legislation for RWW reuse, 2. evaluating the effects of RWW irrigation on some ornamental, 3. quantifying fertilizing value and environmental impacts of RWW irrigation. In this paper the results of the last year experimental activity are presented. Experimental results indicated that filtration and disinfection with peracetic acid plus UV were very effective in indicator bacteria removal; as a matter of fact, neither Escherichia coli nor Total Coliforms were detected in any samples of pilot plant effluent. Results of an agronomical experiment indicated the suitability of the tertiary effluent for the irrigation: at the end of the growing season a higher (or equal) total dry mass was detected in RWW irrigated plants than well-water (WW) irrigated ones. Finally, the tertiary effluent can be considered as an important source of fertilizer for container-grown plants. As a matter of fact RWW irrigation showed to be able to replace at least one of the traditional fertilization treatments.

COMPARISON OF CONVENTIONAL AND Vibrio fischeri BIOASSAYS FOR THE ASSESSMENT OF MUNICIPAL WASTEWATER TOXICITY

2012 ◽  
Vol 11 (11) ◽  
pp. 2073-2076 ◽  
Author(s):  
Nora Kovats ◽  
Maha Refaey ◽  
Bettina Varanka ◽  
Karoly Reich ◽  
Arpad Ferincz ◽  
...  
Keyword(s):  
Municipal Wastewater ◽  
Vibrio Fischeri

Costs of tertiary treatment of municipal wastewater by rapid sand filter with coagulants and UV

2003 ◽  
Vol 3 (4) ◽  
pp. 145-152 ◽  
Author(s):  
H. Heinonen-Tanski ◽  
P. Juntunen ◽  
R. Rajala ◽  
E. Haume ◽  
A. Niemelä
Keyword(s):  
Total Phosphorus ◽  
Municipal Wastewater ◽  
Pilot Scale ◽  
Treated Wastewater ◽  
Tertiary Treatment ◽  
Sand Filter ◽  
Physico Chemical ◽  
Bathing Waters ◽  
Filtration Unit ◽  
Microbial Groups

Municipal treated wastewater has been tertiary treated in a pilot-scale rapid sand filter. The filtration process was improved by using polyaluminium coagulants. The sand-filtered water was further treated with one or two UV reactors. The quality changes of wastewater were measured with transmittance, total phosphorus, soluble phosphorus, and somatic coliphages, FRNA-coliphages, FC, enterococci and fecal clostridia. Sand filtration alone without coagulants improved slightly some physico-chemical parameters and it had almost no effect on content of microorganisms. If coagulants were used, the filtration was more effective. The reductions were 88-98% for microbial groups and 80% for total phosphorus. The wastewater would meet the requirements for bathing waters (2,000 FC/100 ml, EU, 1976). UV further improved the hygiene level; this type of treated wastewater could be used for unrestricted irrigation (2.2 TC/100 ml, US.EPA 1992). The improvement was better if coagulants were used. The price for tertiary treatment (filtration + UV) would have been 0.036 Euro/m3 according to prices in 2001 in 22 Mm3/a. The investment cost needed for the filtration unit was 0.020 Euro/m3 (6%/15a). Filtration with coagulants is recommended in spite of its costs, since the low transmittance of unfiltered wastewater impairs the efficiency of the UV treatment.

scholarly journals Occurrence of SARS-CoV-2 RNA in Six Municipal Wastewater Treatment Plants at the Early Stage of COVID-19 Pandemic in The United States

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 798
Author(s):  
Samendra P. Sherchan ◽  
Shalina Shahin ◽  
Jeenal Patel ◽  
Lauren M. Ward ◽  
Sarmila Tandukar ◽  
...  
Keyword(s):  
United States ◽  
Wastewater Treatment ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Quantitative Polymerase Chain Reaction ◽  
The United States ◽  
Secondary Effluent ◽  
Untreated Wastewater ◽  
Treatment Processes ◽  
Wastewater Samples

In this study, we investigated the occurrence of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) RNA in primary influent (n = 42), secondary effluent (n = 24) and tertiary treated effluent (n = 34) collected from six wastewater treatment plants (WWTPs A–F) in Virginia (WWTP A), Florida (WWTPs B, C, and D), and Georgia (WWTPs E and F) in the United States during April–July 2020. Of the 100 wastewater samples analyzed, eight (19%) untreated wastewater samples collected from the primary influents contained SARS-CoV-2 RNA as measured by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays. SARS-CoV-2 RNA were detected in influent wastewater samples collected from WWTP A (Virginia), WWTPs E and F (Georgia) and WWTP D (Florida). Secondary and tertiary effluent samples were not positive for SARS-CoV-2 RNA indicating the treatment processes in these WWTPs potentially removed SARS-CoV-2 RNA during the secondary and tertiary treatment processes. However, further studies are needed to understand the log removal values (LRVs) and transmission risks of SARS-CoV-2 RNA through analyzing wastewater samples from a wider range of WWTPs.

scholarly journals Prevalence of Antibiotic-Resistant Bacteria ESKAPE among Healthy People Estimated by Monitoring of Municipal Wastewater

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 495
Author(s):  
Masateru Nishiyama ◽  
Susan Praise ◽  
Keiichi Tsurumaki ◽  
Hiroaki Baba ◽  
Hajime Kanamori ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
General Population ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Municipal Wastewater Treatment ◽  
Antibiotic Resistant ◽  
Carbapenem Resistant ◽  
Wastewater Samples

There is increasing attention toward factors that potentially contribute to antibiotic resistance (AR), as well as an interest in exploring the emergence and occurrence of antibiotic resistance bacteria (ARB). We monitored six ARBs that cause hospital outbreaks in wastewater influent to highlight the presence of these ARBs in the general population. We analyzed wastewater samples from a municipal wastewater treatment plant (MWWTP) and hospital wastewater (HW) for six species of ARB: Carbapenem-resistant Enterobacteria (CARBA), extended-spectrum β-lactamase producing Enterobacteria (ESBL), multidrug-resistant Acinetobacter (MDRA), multidrug-resistant Pseudomonas aeruginosa (MDRP), methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant Enterococci (VRE). We registered a high percentage of ARBs in MWWTP samples (>66%) for all ARBs except for MDRP, indicating a high prevalence in the population. Percentages in HW samples were low (<78%), and no VRE was detected throughout the study. CARBA and ESBL were detected in all wastewater samples, whereas MDRA and MRSA had a high abundance. This result demonstrated the functionality of using raw wastewater at MWWTP to monitor the presence and extent of ARB in healthy populations. This kind of surveillance will contribute to strengthening the efforts toward reducing ARBs through the detection of ARBs to which the general population is exposed.

Evaluation of the California Wastewater Reclamation Criteria Using Enteric Virus Monitoring Data

1992 ◽  
Vol 26 (7-8) ◽  
pp. 1513-1524 ◽  
Author(s):  
T. Asano ◽  
L. Y. C. Leong ◽  
M. G. Rigby ◽  
R. H. Sakaji
Keyword(s):  
Municipal Wastewater ◽  
Enteric Virus ◽  
Monitoring Data ◽  
Municipal Wastewater Treatment ◽  
Wastewater Reclamation ◽  
Food Crop ◽  
Reclaimed Wastewater ◽  
Ground Water Recharge ◽  
Wastewater Reclamation And Reuse ◽  
Crop Irrigation

The State of California's WastewaterReclamationCriteria is under review and will be revised and expanded to include several new regulations on the use of reclaimed municipal wastewater. To provide a scientific basis for the evaluation of the existing and proposed Criteria, enteric virus monitoring data from secondary and tertiary effluents were evaluated. These virus data were obtained from special studies and monitoring reports, covering the period from 1975 to 1989, including ten municipal wastewater treatment facilities in California. Based on the enteric virus data from these reports, and using the current Criteria as a guide, four exposure scenarios were developed to determine the risk of waterborne enteric virus infection to humans as a consequence of wastewater reclamation and reuse. The exposure assessments included food crop irrigation, landscape irrigation for golf courses, recreational impoundments, and ground water recharge. The virus enumeration and the resulting risk assessments described in this paper provide a comparative basis for addressing the treatment and fate of enteric viruses in wastewater reclamation and reuse. The analyses show that annual risk of infection from exposure to chlorinated tertiary effluent containing 1 viral unit/100 L in recreational activities such as swimming or golfing is in the range of 10−2 to 10−7, while exposures resulting from food-crop irrigation or groundwater recharge with reclaimed municipal wastewater is in the range of 10−6 to 10−11. The risk analyses are also used to demonstrate that the probability of infection can be further mitigated by controlling exposure to reclaimed wastewater in the use area.

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