scholarly journals Effect of low concentrations of dissolved oxygen on the activity of denitrifying methanotrophic bacteria

2018 ◽  
Vol 77 (11) ◽  
pp. 2589-2597 ◽  
Author(s):  
Christel Kampman ◽  
Laura Piai ◽  
Hardy Temmink ◽  
Tim L. G. Hendrickx ◽  
Grietje Zeeman ◽  
...  

Abstract Chemical energy can be recovered from municipal wastewater as biogas through anaerobic treatment. Effluent from direct anaerobic wastewater treatment at low temperatures, however, still contains ammonium and considerable amounts of dissolved methane. After nitritation, methane can be used as electron donor for denitrification by the anaerobic bacterium ‘Candidatus Methylomirabilis oxyfera’. It was shown that in the presence of 0.7% O2, denitrifying methanotrophic activity slightly increased and returned to its original level after oxygen had been removed. At 1.1% O2, methane consumption rate increased 118%, nitrite consumption rate increased 58%. After removal of oxygen, methane consumption rate fully recovered, and nitrite consumption rate returned to 88%. Therefore, traces of oxygen that bacteria are likely to be exposed to in wastewater treatment are not expected to negatively affect the denitrifying methanotrophic process. 2.0% O2 inhibited denitrifying activity. Nitrite consumption rate decreased 60% and did not recover after removal of oxygen. No clear effect on methane consumption was observed. Further studies should evaluate if intermittent addition of oxygen results in increased growth rates of the slow-growing ‘Candidatus Methylomirabilis oxyfera’.

Processes ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 1546
Author(s):  
Monika Vítězová ◽  
Anna Kohoutová ◽  
Tomáš Vítěz ◽  
Nikola Hanišáková ◽  
Ivan Kushkevych

Over the past decades, anaerobic biotechnology is commonly used for treating high-strength wastewaters from different industries. This biotechnology depends on interactions and co-operation between microorganisms in the anaerobic environment where many pollutants’ transformation to energy-rich biogas occurs. Properties of wastewater vary across industries and significantly affect microbiome composition in the anaerobic reactor. Methanogenic archaea play a crucial role during anaerobic wastewater treatment. The most abundant acetoclastic methanogens in the anaerobic reactors for industrial wastewater treatment are Methanosarcina sp. and Methanotrix sp. Hydrogenotrophic representatives of methanogens presented in the anaerobic reactors are characterized by a wide species diversity. Methanoculleus sp., Methanobacterium sp. and Methanospirillum sp. prevailed in this group. This work summarizes the relation of industrial wastewater composition and methanogen microbial communities present in different reactors treating these wastewaters.


Complexity ◽  
2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Zehua Huang ◽  
Renren Wu ◽  
XiaoHui Yi ◽  
Hongbin Liu ◽  
Jiannan Cai ◽  
...  

The anaerobic treatment process is a complicated multivariable system that is nonlinear and time varying. Moreover, biogas production rates are an important indicator for reflecting operational performance of the anaerobic treatment system. In this work, a novel model fuzzy wavelet neural network based on the genetic algorithm (GA-FWNN) that combines the advantages of the genetic algorithm, fuzzy logic, neural network, and wavelet transform was established for prediction of effluent quality and biogas production rates in a full-scale anaerobic wastewater treatment process. Moreover, the dataset was preprocessed via a self-adapted fuzzy c-means clustering before training the network and a hybrid algorithm for acquiring the optimal parameters of the multiscale GA-FWNN for improving the network precision. The analysis results indicate that the FWNN with the optimal algorithm had a high speed of convergence and good quality of prediction, and the FWNN model was more advantageous than the traditional intelligent coupling models (NN, WNN, and FNN) in prediction accuracy and robustness. The determination coefficients R2 of the FWNN models for predicting both the effluent quality and biogas production rates were over 0.95. The proposed model can be used for analyzing both biogas (methane) production rates and effluent quality over the operational time period, which plays an important role in saving energy and eliminating pollutant discharge in the wastewater treatment system.


1986 ◽  
Vol 18 (12) ◽  
pp. 41-53 ◽  
Author(s):  
Look Hulshoff Pol ◽  
Gatze Lettinga

Presently anaerobic wastewater treatment is becoming an accepted simple technology for the treatment of a variety of wastewaters. Of the different treatment systems that have been developed the UASB process (Upflow Anaerobic Sludge Blanket) has found the widest application. Almost all of the more than 60 full scale UASB reactors in operation now, are running satisfactorily. The excellent sludge retention generally found in UASB-reactors is obtained by sludge granulation, which can be seen as a sludge immobilization process. The presently available insight into the sludge granulation process is briefly presented, together with the strategy to be applied for performing a proper first start-up and secondary start-up of UASB reactors, viz. using granular seed sludge. The effect of the presence of SS with regard to the loading potentials of anaerobic treatment systems will be discussed. The experiences obtained with some full scale applications of the UASB-process are presented.


2008 ◽  
Vol 58 (2) ◽  
pp. 379-384 ◽  
Author(s):  
D. Weichgrebe ◽  
I. Urban ◽  
K. Friedrich

The classical municipal wastewater treatment in Germany consists of an aerobic carbon and nitrogen elimination and mostly an anaerobic sludge treatment. Organic kitchen wastes from separate waste collection as well as yard wastes are converted mostly in composting plants to soil conditioner. With these conventional types of treatment, the energy potential in waste and wastewater is lost due to aerobic material conversion. In this article three scenarios for the treatment of municipal wastewater and waste are compared on the subject of energy efficiency and useable potential: Sc1. the classical wastewater treatment and the composting of the organic waste fraction, Sc2. the anaerobic treatment of wastewater combined with deammonification and the digestion of the organic waste fraction, and Sc3. a mutual anaerobic treatment of wastewater and waste as co-digestion with deammonification. The calculation of energy and CO2-balance considers different climatic conditions. In case of using anaerobic treatment, not only the energy balance will be positive, also the CO2-balance is improved by the substitution of fossil fuels with generated biogas.


2019 ◽  
Vol 86 ◽  
pp. 00020
Author(s):  
Zbigniew Mucha ◽  
Włodzimierz Wójcik ◽  
Michał Polus

In recent years, anaerobic membrane bioreactor (AnMBR) technology has been considered as a very appealing alternative for wastewater treatment due to its significant advantages over conventional anaerobic treatment and aerobic membrane bioreactor (MBR) technology. The paper provides an overview of the current status of the anaerobic membrane bioreactor technology with a special emphasis on its performance and drawbacks when applied for domestic and municipal wastewater treatment. According to the reported data, the renewable energy produced at the plants (i.e. from methane) covered the energy demand for membrane filtration while the excess energy can be further utilized. Anaerobic membrane bioreactors are an attractive technology that needs further research efforts and applications at an industrial scale.


1999 ◽  
Vol 40 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Pavel Jeníček ◽  
Michal Dohányos ◽  
Jana Zábranská

One of the most recent and progressive reactor principles used in anaerobic wastewater treatment technology is a vertical compartmentalization, which is used in the USSB (Upflow Staged Sludge Bed) reactor. Thanks to its specific design and features the operation of such a reactor can be very flexible. Examples are given showing tested alternatives of operation with combined wastewater and sludge treatment. A high treatment efficiency and a very low specific sludge production was achieved with the operation of a technological system consisting of a USSB reactor and an aerobic biofilm reactor. In the USSB reactor wastewater and surplus sludge treatment can not only be combined but also anaerobic pretreatment and biological denitrification.


1997 ◽  
Vol 35 (9) ◽  
pp. 191-198 ◽  
Author(s):  
Yu Hanqing ◽  
Joo-Hwa Tay ◽  
Francis Wilson

In this paper, a feasible municipal wastewater treatment process, using the upflow anaerobic sludge blanket (UASB) or the anaerobic baffled reactor (ABR) as an anaerobic pre-treatment system, and the reed bed or the stabilization pond with supporting media as a post-treatment system, is presented and discussed. Results obtained in pilot- and full-scale treatment plants clearly reveal that the anaerobic treatment is indeed a very attractive option for municipal wastewater pre-treatment at temperatures exceeding 20C in tropical and subtropical regions. The UASB system has been commonly employed as an anaerobic pre-treatment system. The ABR provides another potential for the anaerobic pre-treatment. The effluents from the anaerobic treatment system should be post-treated to meet discharge standards. Because of the advantages of the reed bed system when it is employed for tertiary treatment, this system could be considered as a post-treatment system. Another cost-effective system, the stabilization pond packed with attached-growth media, is also a potential post-treatment system.


1991 ◽  
Vol 24 (8) ◽  
pp. 61-78 ◽  
Author(s):  
S. R. Harper ◽  
M. T. Suidan

A discussion of principles guiding the experimental elucidation of microbial growth and substrate utilization kinetics, and the development of mathematical models for anaerobic wastewater treatment systems, is presented. Recent developments in the areas of anaerobic treatment microbiology (including biomass characterization), biochemical mechanisms, microbial growth dyamics, reactor hydrodynamics, and mathematical modeling approaches are presented and explored.


Author(s):  
Victoria Mel’nick ◽  
Olha Vorobyova ◽  
Natalia Ostapenko

Analysis of literature sources suggests that the use of anaerobic treatment methods is especially effective for high concentrations of contaminants in wastewater, as well as for large volumes of water supplied for treatment, which is typical for industrial wastewater. In biotechnological production and food industry, waste water has a high level of pollution with organic compounds, it is advisable to use anaerobic treatment methods. Anaerobic wastewater treatment processes are characterized by low energy consumption and the ability to recover energy through the combustion of methane. To ensure the required temperature regime for the operation of the bioreactor, a new design of the heat exchange device of the anaerobic bioreactor with immobilized microorganisms has been developed. This design of the bioreactor can provide the required degree of wastewater purification at significantly lower costs for construction, operation, and material and energy resources. For clarity, the design features were designed bioreactor with a plane load of bundled software SOLIDWORKS and a mathematical model of the processes of anaerobic wastewater treatment.


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