STATE OF THE HUMAN INNATE IMMUNITY CELL COMPONENT DURING 120-DAY ISOLATION IN A PRESSURIZED MODULE

2021 ◽  
Vol 55 (2) ◽  
pp. 35-42
Author(s):  
S.A. Ponomarev ◽  
◽  
S.M. Shulguina ◽  
S.A. Kalinin ◽  
E.N. Antropova ◽  
...  
Keyword(s):  
Pattern Recognition ◽  
Innate Immunity ◽  
Peripheral Blood ◽  
Dynamic Balance ◽  
Controlled Environment ◽  
Natural Killers ◽  
Cell Component ◽  
Blood Samples ◽  
Extreme Factors ◽  
Primary Activation

Innate immunity was investigated in six 28 to 44 y.o. volunteers for 120-d isolation in controlled environment. Peripheral blood samples gathered during isolation were analyzed to determine monocytes and granulocytes expressing intracellular (TLR3, TLR8, TLR9) and surface (TLR1, TLR2, TLR4, TLR5, TLR6) TLRs, and lymphocytes-natural killers (CD3-CD16+CD56+). The system of pattern-recognition cell receptors displayed a heterogenic reaction with primary activation throughout the period in isolation. The observed changes represent evidently the processes of establishing a dynamic balance of immunity with extreme factors of the life in isolation and confinement, including the reactions to endogenous and exogenous stimuli.

scholarly journals Evaluation of T cell-mediated antitumor immune response in liver tumors - an approach using tumor and peripheral blood samples

HPB ◽  
2018 ◽  
Vol 20 ◽  
pp. S262
Author(s):  
R. Martins ◽  
C. Martín-Sierra ◽  
P. Laranjeira ◽  
A.M. Abrantes ◽  
J.G. Tralhão ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cell ◽  
Peripheral Blood ◽  
Liver Tumors ◽  
Antitumor Immune Response ◽  
Blood Samples

scholarly journals Increased Density of Human Immunodeficiency Virus Type 1 Coreceptors CCR5 and CXCR4 on the Surfaces of CD4+ T Cells and Monocytes of Patients with Schistosoma mansoni Infection

2003 ◽  
Vol 71 (11) ◽  
pp. 6668-6671 ◽  
Author(s):  
W. Evan Secor ◽  
Amil Shah ◽  
Pauline M. N. Mwinzi ◽  
Bryson A. Ndenga ◽  
Caroline O. Watta ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
T Cells ◽  
Cell Surface ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Peripheral Blood ◽  
Chemokine Receptors ◽  
Blood Samples ◽  
Immunodeficiency Virus

ABSTRACT Distribution of chemokine receptors CCR5 and CXCR4, which are also coreceptors for human immunodeficiency virus type 1 invasion of cells, was measured on the surfaces of CD4+ T cells and monocytes in peripheral blood samples from a group of Kenyan car washers. Patients with active schistosomiasis displayed higher cell surface densities of these receptors than did cured schistosomiasis patients.

Evaluation of the tshr gene reveals polymorphisms associated with typical symptoms in primary congenital hypothyroidism

2016 ◽  
Vol 29 (1) ◽  
Author(s):  
Erik Artur Cortinhas Alves ◽  
Raissa Coelho Andrade ◽  
Carlos Eduardo de Melo Amaral ◽  
Milena Coelho Fernandes Caldato ◽  
Adriana Maria Rocha Bastos ◽  
...  
Keyword(s):  
Congenital Hypothyroidism ◽  
Peripheral Blood ◽  
Genomic Dna ◽  
Gene Mutations ◽  
Molecular Heterogeneity ◽  
Coding Region ◽  
Blood Samples ◽  
Live Births ◽  
Tshr Gene ◽  
Inactivating Mutations

AbstractPrimary congenital hypothyroidism (PCH) has an incidence of approximately 1 in each 3000–4000 live births. In the last two decades, nearly 50 types of the distinct inactivating mutations have already been described in the coding region of the tshr gene. The aim of present study was to investigate tshr gene mutations in patients with primary congenital hypothyroidism, analyzing a sample of 106 patients that were diagnosed with PCH. Genomic DNA was isolated from peripheral blood samples, and 10 exons from the TSH receptor were automatically sequenced. Five nucleotide alterations (P52T, N187N, A459A, L645L, and D727E. N187N and D727E polymorphisms) were associated with positive medical history. In view of the clinical, biochemical and molecular heterogeneity of the etiology of the PCH, the study of polymorphisms is critical for investigating the possible associations with prevailing symptoms of this disorder.

scholarly journals Associations between the Epithelial-Mesenchymal Transition Phenotypes of Circulating Tumor Cells and the Clinicopathological Features of Patients with Colorectal Cancer

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Fengjie Wu ◽  
Jun Zhu ◽  
Yongjiang Mao ◽  
Xiaomei Li ◽  
Baoguang Hu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Peripheral Blood ◽  
Epithelial Mesenchymal Transition ◽  
Mesenchymal Cell ◽  
Blood Samples ◽  
Mesenchymal Transition ◽  
Enrichment Technique ◽  
Tumor Node Metastasis Stage ◽  
Mesenchymal Cell Marker ◽  
Circulating Tumor Microemboli

In this study, we identified CTCs using the previously reported CanPatrol CTC enrichment technique from peripheral blood samples of 126 patients with colorectal cancer (CRC) and found that CTCs could be classified into three subpopulations based on expression of epithelial cell adhesion molecule (EpCAM) (E-CTCs), the mesenchymal cell marker vimentin (M-CTCs), or both EpCAM and vimentin (biphenotypic E/M-CTCs). Circulating tumor microemboli (CTMs) were also identified in peripheral blood samples. Meanwhile, E-CTCs, M-CTCs, E/M-CTCs, and CTMs were detected in 76.98%, 42.06%, 56.35%, and 36.51% of the 126 patients, respectively. Interestingly, the presence of CTMs and each CTC subpopulation was significantly associated with blood lymphocyte counts and tumor-node-metastasis stage (P<0.001). Lymphocyte counts and the neutrophil-to-lymphocyte ratio (NLR) in patients lacking CTCs were significantly different from those in patients testing positive for CTMs and each CTC subpopulation (P<0.001). Our results indicate that tumor metastasis is more significantly associated with the presence of CTMs and M-CTCs than with other CTC subpopulations and suggest that EMT may be involved in CTC evasion of lymphocyte-mediated clearance.

Development of Implantable Autologous Small-Calibre Vascular Grafts from Peripheral Blood Samples

2013 ◽  
Vol 138 (02) ◽  
pp. 173-179 ◽  
Author(s):  
T. Aper ◽  
O. Teebken ◽  
A. Krüger ◽  
A. Heisterkamp ◽  
A. Hilfiker ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Vascular Grafts ◽  
Blood Samples

scholarly journals Earlier Positivity of Central-Venous- versus Peripheral-Blood Cultures Is Highly Predictive of Catheter-Related Sepsis

1998 ◽  
Vol 36 (1) ◽  
pp. 105-109 ◽  
Author(s):  
François Blot ◽  
Eric Schmidt ◽  
Gérard Nitenberg ◽  
Cyrille Tancrède ◽  
Bernard Leclercq ◽  
...  
Keyword(s):  
Central Venous Catheter ◽  
Peripheral Blood ◽  
Venous Catheter ◽  
Blood Cultures ◽  
Central Venous ◽  
Indwelling Catheter ◽  
Blood Samples ◽  
Reference Center ◽  
Reliable Tool ◽  
Period Data

To diagnose catheter-related sepsis without removing the catheter, we evaluated the differential positivity times of cultures of blood drawn simultaneously from central venous catheter and peripheral sites. In a 450-bed cancer reference center, simultaneous central- and peripheral-blood cultures were prospectively performed for patients with suspicion of catheter-related sepsis over an 18-month period. Data for 64 patients for whom the same microorganisms were found when central- and peripheral-blood samples were cultured were retrospectively reviewed by two independent physicians blinded to the differential positivity time values in order to establish or refute the diagnosis of catheter-related sepsis. The diagnosis was established in 28 cases, refuted in 14, and indeterminate in the remaining 22. The differential positivity time was significantly greater for patients with catheter-related sepsis (P < 10−4). A cutoff limit of +120 min had 100% specificity and 96.4% sensitivity for the diagnosis of catheter-related sepsis. These results strongly suggest that measurement of the differential positivity time might be a reliable tool facilitating the diagnosis of catheter-related sepsis in patients with an indwelling catheter.

Quantitative detection of circulating renal cancer cells in peripheral blood samples: Experience of a two-center study

2003 ◽  
Vol 2 (1) ◽  
pp. 179
Author(s):  
A. Meye ◽  
U. Bilkenroth ◽  
U. Schmidt ◽  
Ms. Blümke ◽  
S. Füssel ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Renal Cancer ◽  
Peripheral Blood ◽  
Quantitative Detection ◽  
Blood Samples ◽  
Center Study

scholarly journals Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules

2021 ◽  
Author(s):  
Matteo Stravalaci ◽  
Isabel Pagani ◽  
Elvezia Maria Paraboschi ◽  
Mattia Pedotti ◽  
Andrea Doni ◽  
...  
Keyword(s):  
Pattern Recognition ◽  
Innate Immunity ◽  
Disease Severity ◽  
Fluid Phase ◽  
Systematic Investigation ◽  
In Vitro Models ◽  
Lectin Pathway ◽  
Mannose Binding ◽  
Binding Lectin

The humoral arm of innate immunity includes diverse molecules with antibody-like functions, some of which serve as disease severity biomarkers in COVID-19. The present study was designed to conduct a systematic investigation of the interaction of humoral fluid phase pattern recognition molecules (PRM) with SARS-CoV-2. Out of 10 PRM tested, the long pentraxin PTX3 and Mannose Binding Lectin (MBL) bound the viral Nucleoprotein and Spike, respectively. MBL bound trimeric Spike, including that of variants of concern, in a glycan-dependent way and inhibited SARS-CoV-2 in three in vitro models. Moreover, upon binding to Spike, MBL activated the lectin pathway of complement activation. Genetic polymorphisms at the MBL locus were associated with disease severity. These results suggest that selected humoral fluid phase PRM can play an important role in resistance to, and pathogenesis of, COVID-19, a finding with translational implications.

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