scholarly journals Efficacy of Tithonia diversifolia (Hemsl) A. Gray on the inhibition of larval development of Haemonchus contortus

2020 ◽  
Vol 14 (3) ◽  
pp. 191-195
Author(s):  
Eduardo Robson Duarte ◽  
Pedro Paulo Dias David ◽  
Katchuce Brito Amarante Oliveria ◽  
Marcelo Dourado Lima ◽  
Fernando dos Santos Magaço ◽  
...  
Keyword(s):  
Larval Development ◽  
Haemonchus Contortus ◽  
Tithonia Diversifolia

scholarly journals The effects of flubendazole and its metabolites on the larval development of Haemonchus contortus (Nematoda: Trichostrongylidae): an in vitro study

2010 ◽  
Vol 47 (4) ◽  
pp. 269-272 ◽  
Author(s):  
H. Bártíková ◽  
L. Skálová ◽  
J. Lamka ◽  
B. Szotáková ◽  
M. Várady
Keyword(s):  
Larval Development ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
In Vitro Study ◽  
Lethal Concentration ◽  
Resistant Isolate ◽  
Susceptible Isolate ◽  
Marked Resistance ◽  
Larval Development Test

AbstractThe anthelmintic effects of flubendazole (FLU), its two main metabolites reduced flubendazole (FLU-R) and hydrolyzed flubendazole (FLU-H), and thiabendazole (TBZ) were compared using an in vitro larval development test in two isolates of Haemonchus contortus, a fully susceptible isolate (HCS) and a multi-resistant isolate (HCR). Results were quantified as 50 % lethal concentration (LC50), 99 % lethal concentration (LC99), efficacy factor (EF), and resistance factor (RF). For HCS, both LC50 and LC99 of FLU were lower than those of the reference TBZ. The anthelmintic activity of FLU-R in HCS and HCR was 13 and 6 times lower than the activity of FLU, respectively. The anthelmintic activity of FLU-H was negligible (approximately 363–853 times lower) compared to that of FLU. Although a marked resistance of the HCR isolate to TBZ was confirmed, only a low tolerance to FLU-R and slightly higher tolerance to FLU were found.

scholarly journals Dafachronic acid promotes larval development in Haemonchus contortus by modulating dauer signalling and lipid metabolism

2019 ◽  
Vol 15 (7) ◽  
pp. e1007960 ◽  
Author(s):  
Guangxu Ma ◽  
Tao Wang ◽  
Pasi K. Korhonen ◽  
Neil D. Young ◽  
Shuai Nie ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Larval Development ◽  
Haemonchus Contortus ◽  
Dafachronic Acid

scholarly journals Anthelmintic Activity of Hymenodictyon pachyanta Stem Bark Extracts against Haemonchus Contortus

2019 ◽  
Vol 63 (2) ◽  
pp. 30-36
Author(s):  
D. O. Olayemi ◽  
M. M. Onakpa ◽  
O. C. Jegede
Keyword(s):  
Larval Development ◽  
Crude Extract ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Stem Bark ◽  
Parasitic Nematodes ◽  
Egg Hatching ◽  
Crude Extracts ◽  
Significant Difference

Abstract The development of host resistance to anthelmintics and the increasing cost of commercial anthelmintics have encouraged the need for the in vitro anthelmintic evaluation of crude extract and fractions of Hymenodictyon pachyanta plant as alternative drugs against Haemonchus contortus. H. contortus is one of the most prevalent and highly pathogenic parasitic nematodes in small ruminant farming globally. H. pachyanta stem bark is a prospective plant used by the local and indigenous farmers of Nsukka, Enugu state, Nigeria. The stem bark of H. pachyanta were collected, dried, pulverized and extracted with 80 % methanol. The purpose of this study was to investigate the in vitro anthelmintic effects of these crude extract and fractions against H. contortus in sheep and goats. The two extracts (crude and fractions) of H. pachyanta were tested by the egg hatch assay (EHA) and the larval development inhibition assays (LDIA) and to compared the results with albendazole (as the positive control). The concentrations for the crude extract and albendazole used for this study were 0.78, 1.56, 3.125, 6.25 and 12.5 mg.ml−1. The results demonstrated that the crude extracts, fractions and albendazole all at the concentration doses of 12.5 mg.ml−1 produced 100 % inhibition of egg hatching and larval development. Statistically, there was no significant difference (P > 0.05) in the mean percentage inhibition of egg hatching and larval development inhibition of the crude extracts and fractions when compared with albendazole. However, a significant difference (P < 0.05) was observed with n-butanol fraction which inhibited 96.17 % of egg hatchability. All of the extracts and albendazole showed ovicidal and larvicidal effects and were able to induce over 50 % of the egg hatching and mortality of larvae at the concentration ranges of 0.78—12.5 mg.ml−1. The results obtained from our study suggest that H. pachyanta had ovicidal and larvicidal activity against H. contortus and that the bioactive plants compounds responsible for this effect could be attributed to the presence of tannins, alkaloids and the saponins contained in the crude extracts.

scholarly journals PERKEMBANGAN DAN GAMBARAN ANATOMIS LARVA INFEKTIF (L3) Haemonchus contortus YANG DIBIAKKAN DENGAN VERMICULLITE (Development and Anatomical Description of Infektive Larvae (L3) Haemonchus contortus Cultured with Vermicullite)

2016 ◽  
Vol 10 (1) ◽  
pp. 62
Author(s):  
Rahmi Sulastri Mukhtar ◽  
Muhammad Hambal ◽  
Muhammad Hanafiah ◽  
Yudha Fahrimal ◽  
Winaruddin Winaruddin ◽  
...  
Keyword(s):  
Larval Development ◽  
Body Length ◽  
Haemonchus Contortus ◽  
Tail Length ◽  
Total Body Length ◽  
Basic Information ◽  
Infective Larvae ◽  
Body Diameter ◽  
Total Body ◽  
Banda Aceh

This study was aimed at obtaining basic information about bioecology of Haemonchus contortus, related to larval development and anatomical description of infective larvae (L3). In this study, the ova of Haemonchus contortus were harvested out of the intestines of goats, those were slaughtered in municipal slaughterhouse in Kampung Jawa Banda Aceh. The ova of Haemonchus contortus were then cultured using vermiculite as media, and observed daily until the larvae reached 3rd stage larvae. The result revealed that the larvae need 7 days to reach from eggs into infective larvae. Larvae obtained has uniform morphology, with a body diameter of 22 μm, tail length up to 55 μm hyaline, esophagus posterior to the tail of 592 μm, length of esophagus anterior to posterior esophagus 145 μm, and a total body length of larvae 737 μm.

Comparison of two versions of larval development test to detect anthelmintic resistance in Haemonchus contortus

2009 ◽  
Vol 160 (3-4) ◽  
pp. 267-271 ◽  
Author(s):  
Marián Várady ◽  
Július Čorba ◽  
Valéria Letková ◽  
Gabriel Kováč
Keyword(s):  
Larval Development ◽  
Haemonchus Contortus ◽  
Anthelmintic Resistance ◽  
Larval Development Test

Studies on parasitic worms of sheep in Scotland

Parasitology ◽  
1959 ◽  
Vol 49 (1-2) ◽  
pp. 23-38 ◽  
Author(s):  
Paul H. Silverman ◽  
J. Allan Campbell
Keyword(s):  
Larval Development ◽  
Haemonchus Contortus ◽  
Laboratory Studies ◽  
Moisture Conditions ◽  
Parasitic Worms ◽  
Embryonic And Larval Development

Laboratory studies on the embryonic and larval development of Haemonchus contortus under constant temperatures of 0·0°, 7·2°, 11°, 14·4°, 21·7° and 37° C., and varying moisture conditions were carried out. Reports of preliminary studies on Ostertagia spp., Trichostrongylus spp. and Oesophagostomum venulosum are included.Observations showed that eggs in the same culture may behave in several ways: (1) eggs may develop at different rates; (2) eggs may exhibit intermittent stages of active and inhibited development; and (3) eggs may fail to develop and die at any stage from unknown causes.

scholarly journals Haemonchus contortus Transthyretin-Like Protein TTR-31 Plays Roles in Post-Embryonic Larval Development and Potentially Apoptosis of Germ Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Hengzhi Shi ◽  
Xiaocui Huang ◽  
Xueqiu Chen ◽  
Yi Yang ◽  
Fei Wu ◽  
...  
Keyword(s):  
Larval Development ◽  
Germ Cells ◽  
Haemonchus Contortus ◽  
Gene Knockdown ◽  
Secretory Product ◽  
Pumping Rate ◽  
Nematode Reproduction ◽  
C Elegans ◽  
Transcriptional Pattern ◽  
Nematode Development

Transthyretin (TTR)-like proteins play multi-function roles in nematode and are important component of excretory/secretory product in Haemonchus contortus. In this study, we functionally characterised a secretory transthyretin-like protein in the barber’s pole worm H. contortus. A full-length of transthyretin-like protein-coding gene (Hc-ttr-31) was identified in this parasitic nematode, representing a counterpart of Ce-ttr-31 in Caenorhabditis elegans. High transcriptional levels of Hc-ttr-31 were detected in the egg and early larval stages of H. contortus, with the lowest level measured in the adult stage, indicating a decreased transcriptional pattern of this gene during nematode development. Localisation analysis indicated a secretion of TTR-31 from the intestine to the gonad, suggesting additional roles of Hc-ttr-31 in nematode reproduction. Expression of Hc-ttr-31 and Ce-ttr-31 in C. elegans did not show marked influence on the nematode development and reproduction, whereas Hc-ttr-31 RNA interference-mediated gene knockdown of Ce-ttr-31 shortened the lifespan, decreased the brood size, slowed the pumping rate and inhibited the growth of treated worms. Particularly, gene knockdown of Hc-ttr-31 in C. elegans was linked to activated apoptosis signalling pathway, increased general reactive oxygen species (ROS) level, apoptotic germ cells and facultative vivipary phenotype, as well as suppressed germ cell removal signalling pathways. Taken together, Hc-ttr-31 appears to play roles in regulating post-embryonic larval development, and potentially in protecting gonad from oxidative stress and mediating engulfment of apoptotic germ cells. A better knowledge of these aspects should contribute to a better understanding of the developmental biology of H. contortus and a discovery of potential targets against this and related parasitic worms.

Sign in / Sign up

Export Citation Format

Share Document