scholarly journals Metabolic Engineering of Light and Dark Biochemical Pathways in Wild-Type and Mutant Strains of Synechocystis PCC 6803 for Maximal, 24-Hour Production of Hydrogen Gas

2014 ◽  
Author(s):  
Roger L. Ely ◽  
Frank W.R. Chaplen
Keyword(s):  
Metabolic Engineering ◽  
Hydrogen Gas ◽  
Wild Type ◽  
Synechocystis Pcc 6803 ◽  
Biochemical Pathways ◽  
Production Of Hydrogen ◽  
Mutant Strains ◽  
Pcc 6803

scholarly journals Suppressor Mutations in the Study of Photosystem I Biogenesis: sll0088 Is a Previously Unidentified Gene Involved in Reaction Center Accumulation in Synechocystis sp. Strain PCC 6803

2003 ◽  
Vol 185 (13) ◽  
pp. 3878-3887 ◽  
Author(s):  
Jianping Yu ◽  
Gaozhong Shen ◽  
Tao Wang ◽  
Donald A. Bryant ◽  
John H. Golbeck ◽  
...  
Keyword(s):  
Photosystem I ◽  
Point Mutations ◽  
Kanamycin Resistance ◽  
Negative Regulator ◽  
Binding Motif ◽  
Wild Type ◽  
Photoautotrophic Growth ◽  
Suppressor Mutations ◽  
Mutant Strains ◽  
Pcc 6803

ABSTRACT In previous work, some members of our group isolated mutant strains of Synechocystis sp. strain PCC 6803 in which point mutations had been inserted into the psaC gene to alter the cysteine residues to the FA and FB iron-sulfur clusters in the PsaC subunit of photosystem I (J. P. Yu, I. R. Vassiliev, Y. S. Jung, J. H. Golbeck, and L. McIntosh, J. Biol. Chem. 272:8032-8039, 1997). These mutant strains did not grow photoautotrophically due to suppressed levels of chlorophyll a and photosystem I. In the results described here, we show that suppressor mutations produced strains that are capable of photoautotrophic growth at moderate light intensity (20 μmol m−2 s−1). Two separate suppressor strains of C14SPsaC, termed C14SPsaC-R62 and C14SPsaC-R18, were studied and found to have mutations in a previously uncharacterized open reading frame of the Synechocystis sp. strain PCC 6803 genome named sll0088. C14SPsaC-R62 was found to substitute Pro for Arg at residue 161 as the result of a G482→C change in sll0088, and C14SPsaC-R18 was found to have a three-amino-acid insertion of Gly-Tyr-Phe following Cys231 as the result of a TGGTTATTT duplication at T690 in sll0088. These suppressor strains showed near-wild-type levels of chlorophyll a and photosystem I, yet the serine oxygen ligand to FB was retained as shown by the retention of the S ≥ 3/2 spin state of the [4Fe-4S] cluster. The inactivation of sll0088 by insertion of a kanamycin resistance cartridge in the primary C14SPsaC mutant produced an engineered suppressor strain capable of photoautotrophic growth. There was no difference in psaC gene expression or in the amount of PsaC protein assembled in thylakoids between the wild type and an sll0088 deletion mutant. The sll0088 gene encodes a protein predicted to be a transcriptional regulator with sequence similarities to transcription factors in other prokaryotic and eukaryotic organisms, including Arabidopsis thaliana. The protein contains a typical helix-turn-helix DNA-binding motif and can be classified as a negative regulator by phylogenetic analysis. This suggests that the product of sll0088 has a role in regulating the biogenesis of photosystem I.

Photosystem II activity of wild type Synechocystis PCC 6803 and its mutants with different plastoquinone pool redox states

2016 ◽  
Vol 81 (8) ◽  
pp. 858-870
Author(s):  
O. V. Voloshina ◽  
Y. V. Bolychevtseva ◽  
F. I. Kuzminov ◽  
M. Y. Gorbunov ◽  
I. V. Elanskaya ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Wild Type ◽  
Synechocystis Pcc 6803 ◽  
Redox States ◽  
Plastoquinone Pool ◽  
Photosystem Ii Activity ◽  
Pcc 6803

Interrelation between Cyanophycin Synthesis, L-Arginine Catabolism and Photosynthesis in the Cyanobacterium Synechocystis Sp. Strain PCC 6803

2000 ◽  
Vol 55 (11-12) ◽  
pp. 927-942 ◽  
Author(s):  
Dirk Paul Stephan ◽  
Hans Georg Ruppel ◽  
Elfriede K. Pistorius
Keyword(s):  
Relative Concentration ◽  
Nutrient Deficiency ◽  
Phosphate Limitation ◽  
Physiological Changes ◽  
Wild Type ◽  
Synechocystis Pcc 6803 ◽  
N Source ◽  
Arginine Catabolism ◽  
Synechocystis Sp ◽  
Pcc 6803

ʟ Ultrastructural and imm unocytochemical investigations gave evidence that cyanophycin (multi--arginyl-poly-ʟ-aspartate) granules accumulate in the cyanobacterium Synechocystis sp. strain PCC 6803 under nutrient deficient growth conditions, especially under phosphate limitation. Besides nutrient deficiency, growth of Synechocystis PCC 6803 on ʟ-arginine or ʟ-asparagine as sole N-source also led to high increase of cyanophycin synthesis, while growth on the combination of ʟ-arginine or ʟ-asparagine with nitrate only caused minor cyanophycin accum ulation. Growth of Synechocystis PCC 6803 on ʟ-arginine as sole N-source caused substantial morphological and physiological changes, such as severe thylakoid membrane degradation with partial loss of pigments and photosynthetic activity leading to a phenotype almost like that seen under nutrient deficiency. In contrast to the wild type, the PsbO-free Synechocystis PCC 6803 mutant could grow on ʟ-arginine as sole N-source with only minor morphological and physiological changes. Due to its fairly balanced growth, the mutant accumulated only few cyanophycin granules. ʟ-arginine degrading activity (measured as ornithine and ammonium formation) was high in the PsbO-free mutant but not in the wild type when cells were grown on ʟ-arginine as sole N-source. In both cells types the ʟ-arginine degrading activity was high (although in the PsbO-free mutant about twice as high as in wild type), when cells were grown on ʟ-arginine in combination with nitrate, and as expected very low when cells were grown on nitrate as sole N-source. Thus, net cyanophycin accumulation in Synechocystis PCC 6803 is regulated by the relative concentration of ʟ-arginine to the total nitrogen pool, and the intracellular ʟ-arginine concentration is greatly influenced by the activity of the ʟ-arginine degrading enzyme system which in part is regulated by the activity status of photosystem II. These results suggest a complex interrelation between cyanophycin synthesis, ʟ-arginine catabolism , and in addition photosynthesis in Synechocystis PCC 6803.

scholarly journals Reduction of Photoautotrophic Productivity in the Cyanobacterium Synechocystis sp. Strain PCC 6803 by Phycobilisome Antenna Truncation

2012 ◽  
Vol 78 (17) ◽  
pp. 6349-6351 ◽  
Author(s):  
Lawrence E. Page ◽  
Michelle Liberton ◽  
Himadri B. Pakrasi
Keyword(s):  
Light Harvesting ◽  
Wild Type ◽  
Content Type ◽  
Photosynthetic Productivity ◽  
Mutant Strains ◽  
Light Harvesting Antenna ◽  
Culture Productivity ◽  
Synechocystis Sp ◽  
Pcc 6803

ABSTRACTTruncation of the algal light-harvesting antenna is expected to enhance photosynthetic productivity. The wild type and three mutant strains ofSynechocystissp. strain 6803 with a progressively smaller phycobilisome antenna were examined under different light and CO2conditions. Surprisingly, such antenna truncation resulted in decreased whole-culture productivity for this cyanobacterium.

Characterization of the Light-Induced Oxygen Gas Exchange from the IC 2 Deletion Mutant of Synechocystis PCC 6803 Lacking the Photosystem II 33 kDa Extrinsic Protein

1993 ◽  
Vol 48 (3-4) ◽  
pp. 224-233 ◽  
Author(s):  
V. A. Boichenko ◽  
V. V. Klimov ◽  
S. R. Mayes ◽  
J. Barber
Keyword(s):  
Water Splitting ◽  
Oxygen Evolution ◽  
Oxygen Exchange ◽  
Wild Type ◽  
Exchange Reactions ◽  
Synechocystis Pcc 6803 ◽  
Ps Ii ◽  
Aerobic Conditions ◽  
Ps I ◽  
Pcc 6803

Abstract The absence of the extrinsic Mn-stabilizing 33 kDa protein in the IC 2 mutant of Synechocystis PCC 6803 disturbs the redox cycling of the water splitting system and retards the formation of its higher S-states (I. Vass, K. Cook, S. Deak, S. R. Mayes, and J. Barber, Biochim. Biophys. Acta 1102, 195-201 (1992)). We have performed analyses of the flashinduced oxygen exchange in the mutated cyanobacterium to clarify further the role of the 33 kDa protein. Under aerobic conditions, both the wild type and IC2 mutant show a relatively slow signal of oxygen rise on the first flash which is increased about twice by the addition of 10 μᴍ DCMU and significantly diminished by lowering the oxygen concentration in the medium. According to action spectra measurements, this mode of apparent oxygen release is mediated by PS I and can be attributed to a light induced inhibition of respiratory activity. In contrast to the wild type, having the usual oxygen evolution flash pattern with a periodicity of four, the IC2 mutant shows a binary oscillation pattern of flash-induced respiratory oxygen exchange at a flash frequency 10 Hz, being dampened with DCMU or by a lower flash frequency (< 1 Hz). Oxygen evolution due to water splitting is clearly seen in the IC2 mutant when background far-red illumination is applied to saturate the signal due to respiratory inhibition, but a quadruple oscillatory component of flash-induced oxygen evolution appears only in the presence of artificial electron acceptors under partial aerobic conditions. The mutant possesses a higher PS I/PS II ratio compared to the wild type, as judged from both the flashinduced yields and quantum efficiencies of the steady-state rates of the oxygen exchange reactions. Estimates of antenna sizes indicate about a 20% decrease of optical cross-section at 675 nm of the PS II unit in IC2 mutants in comparison with the wild type. It is suggested that the absence of the 33 kDa protein leads to a modification of the PS II assembly and because of the slowing down of the S-state cycle, the rate of cyclic electron flow around PS II is enhanced. It seems that the absence of the 33 kDa protein in Synechocystis 6803 also disturbs energy transfer between adjacent PS II core complexes and may also alter their association with the phycobilisomes.

Metabolic engineering for enhanced hydrogen production: a review

2013 ◽  
Vol 59 (2) ◽  
pp. 59-78 ◽  
Author(s):  
Yogesh Goyal ◽  
Manish Kumar ◽  
Kalyan Gayen
Keyword(s):  
Metabolic Engineering ◽  
Hydrogen Production ◽  
Hydrogen Gas ◽  
Published Data ◽  
Dual Systems ◽  
Wide Range ◽  
Production Of Hydrogen ◽  
Different Strains ◽  
High Yields ◽  
Associated Species

Hydrogen gas exhibits potential as a sustainable fuel for the future. Therefore, many attempts have been made with the aim of producing high yields of hydrogen gas through renewable biological routes. Engineering of strains to enhance the production of hydrogen gas has been an active area of research for the past 2 decades. This includes overexpression of hydrogen-producing genes (native and heterologous), knockout of competitive pathways, creation of a new productive pathway, and creation of dual systems. Interestingly, genetic mutations in 2 different strains of the same species may not yield similar results. Similarly, 2 different studies on hydrogen productivities may differ largely for the same mutation and on the same species. Consequently, here we analyzed the effect of various genetic modifications on several species, considering a wide range of published data on hydrogen biosynthesis. This article includes a comprehensive metabolic engineering analysis of hydrogen-producing organisms, namely Escherichia coli, Clostridium, and Enterobacter species, and in addition, a short discussion on thermophilic and halophilic organisms. Also, apart from single-culture utilization, dual systems of various organisms and associated developments have been discussed, which are considered potential future targets for economical hydrogen production. Additionally, an indirect contribution towards hydrogen production has been reviewed for associated species.

Phycobilisomes of wild type and pigment mutants of the cyanobacterium Synechocystis PCC 6803

1986 ◽  
Vol 146 (2) ◽  
pp. 186-191 ◽  
Author(s):  
K. Elmorjani ◽  
J. -C. Thomas ◽  
P. Sebban
Keyword(s):  
Wild Type ◽  
Synechocystis Pcc 6803 ◽  
Pcc 6803

Metabolic engineering of cyanobacteria for the production of hydrogen from water

2013 ◽  
Vol 41 (5) ◽  
pp. 1254-1259 ◽  
Author(s):  
Matthias Rögner
Keyword(s):  
Metabolic Engineering ◽  
Water Splitting ◽  
Living Cell ◽  
H2 Production ◽  
Synechocystis Pcc 6803 ◽  
Artificial System ◽  
Physiological Constraints ◽  
Production Of Hydrogen ◽  
Model Cell ◽  
Design Cell

Requirements concerning the construction of a minimal photosynthetic design cell with direct coupling of water-splitting photosynthesis and H2 production are discussed in the present paper. Starting from a cyanobacterial model cell, Synechocystis PCC 6803, potentials and possible limitations are outlined and realization strategies are presented. In extension, the limits of efficiency of all major biological components can be approached in a semi-artificial system consisting of two electrochemically coupled half-cells without the physiological constraints of a living cell.

Sign in / Sign up

Export Citation Format

Share Document