Susceptibility of Intracellular Coxiella burnetii to Antimicrobial Peptides in Mouse Fibroblast Cells

2013 ◽  
Vol 21 (2) ◽  
pp. 115-123 ◽  
Author(s):  
Nathan Unsworth ◽  
Raymond Dawson ◽  
John Wade ◽  
Chun-Qiang Liu
Keyword(s):  
Antimicrobial Peptides ◽  
Coxiella Burnetii ◽  
Mouse Fibroblast ◽  
Fibroblast Cells

Plaque Formation by Chlamydia in L Cells

1970 ◽  
Vol 1 (3) ◽  
pp. 259-262
Author(s):  
Joyce Banks ◽  
B. Eddie ◽  
Julius Schachter ◽  
K. F. Meyer
Keyword(s):  
Cell Lines ◽  
Plaque Assay ◽  
Mouse Fibroblast ◽  
Plaque Formation ◽  
Fibroblast Cells ◽  
Human Origin ◽  
L Cells ◽  
Highly Sensitive ◽  
Slow Growing ◽  
Potential Tool

Chlamydiae were found capable of producing plaques in several cell lines. Mouse fibroblast cells, L-929, proved the most sensitive to infection and yielded plaques of the highest clarity. Assay of chlamydial infectivity by plaque titration was at least as sensitive as egg ld 50 determination. Among chlamydial isolates of avian, mammalian, and human origin, only slow-growing trachoma-inclusion-conjunctivitis agents did not produce plaques. The plaque assay is highly sensitive, reproducible, and offers a potential tool for investigations requiring accurate measurement of small changes in chlamydial infectivity.

scholarly journals Kilham Polyomavirus: Activation of Gene Expression and DNA Replication in Mouse Fibroblast Cells by an Enhancer Substitution

2001 ◽  
Vol 75 (21) ◽  
pp. 10015-10023 ◽  
Author(s):  
Shouting Zhang ◽  
Göran Magnusson
Keyword(s):  
Gene Expression ◽  
Dna Replication ◽  
T Antigen ◽  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
Large T Antigen ◽  
Wild Type ◽  
3T3 Cells ◽  
Late Promoter ◽  
Substitution Mutant

ABSTRACT The Kilham strain of polyomavirus (KV) infects vascular endothelial cells in vivo (J. E. Greenlee, Infect. Immun. 26:705–713, 1979), but no permissive cell type for growth of the virus in vitro has been identified. The failure of KV DNA to replicate in mouse fibroblast cells after transfection suggested that viral gene expression had narrow cell specificity. A KV substitution mutant having a part of the regulatory region of KV DNA replaced with a segment of the polyomavirus transcriptional enhancer was constructed. The substitution mutant was able to replicate in transfected 3T3 cells, and the newly replicated viral DNA associated with protein to form particles with the density of virions in CsCl equilibrium gradients. However, these particles were noninfectious when tested on 3T3 cells, suggesting that absorption or uptake of virus particles was defective for these cells. Analysis of early and late promoter activities by luciferase reporter gene expression showed that the enhancer substitution had a moderate positive effect on early gene expression and a large effect on the expression of the late genes. KV large T antigen inhibited the activities of both the wild-type and the substitution mutant early promoter, whereas only the mutant late promoter was activated under the same conditions. A comparison of the KV and polyomavirus large T antigens showed that they were not interchangeable in the initiation of KV and polyomavirus DNA synthesis. Furthermore, the wild-type KV origin of DNA replication was less active than the mutant structure in the presence of saturating amounts of KV large T antigen. Together, our data demonstrate several differences between the two types of large T antigen in their interactions with cellular proteins.

scholarly journals Identification of natural products and their derivatives as promising inhibitors of protein glycation with non-toxic nature against mouse fibroblast 3T3 cells

2017 ◽  
Vol 8 (4) ◽  
pp. 533 ◽  
Author(s):  
Ghulam Abbas ◽  
Ahmed Suliman Al-Harrasi ◽  
Hidayat Hussain ◽  
Samina Abdul Sattar ◽  
M. Iqbal Choudhary
Keyword(s):  
Acetic Acid ◽  
Significant Inhibition ◽  
Mouse Fibroblast ◽  
Protein Glycation ◽  
Fibroblast Cells ◽  
Inhibition Activity ◽  
3T3 Cells ◽  
Gossypol Acetic Acid ◽  
Deoxypeganine Hydrochloride

<p>This study was performed to identify new inhibitors of protein glycation <em>in vitro</em>. Protein glycation is one of the major causes of late diabetic complications. In this study, terpenoids and alkaloids, isolated from different medicinal plants, along with their derivatives, were evaluated for their antiglycation activity <em>in vitro,</em> while MTT assay on mouse fibroblast 3T3 cells was used to assess their potential cytotoxicity. Among the tested compounds, gossypol (2,2′-<em>bis</em>-(formyl-1,6,7-trihydroxy-5-isopropyl-3-methylnaphthalene) (<strong>1</strong>), isolated from<em> Gossypium herbaceum, </em>and its derivatives,<em> </em>gossypol acetic acid (<strong>2</strong>), gossypolidene- 4-aminoantipyrine (<strong>4</strong>), and gazolidone (<strong>6</strong>), showed a potent antiglycation activity (IC<sub>50</sub> &lt; 16 <em>µ</em>M), while gossypolidene-4-aminoantipyrine (<strong>5</strong>) showed a significant antiglycation activity with IC<sub>50 </sub>value 82.934±2.924<em> µ</em>M, in BSA-fluorescence assay. Alkaloid, noscapine (3S)-6,7-Dimethoxy-3-[(5R)-4-methoxy-6-methyl-5,6,7,8-tetrahy-dro-1,3-dioxolo[4,5-g]isoquinolin-5-yl] isobenzofuran-1(3<em>H</em>)-one (<strong>7</strong>), isolated from <em>Papaver somniferum, N</em>-nitrosoaphyllinic acid (<strong>9</strong>), a derivative of alkaloid aphylline<em>, </em>and 2<em>H</em>-quinolizine, octahydro salt (<strong>11</strong>), a salt of alkaloid lupinine, exhibited significant inhibition activity with<em> </em>IC<sub>50 </sub>values 152.662±5.432, 393.758 ±4.001 µM and 110.203±4.816µM, respectively. Similarly, compounds<strong> </strong>gossypolidene thiocarbamide (<strong>3</strong>), deoxypeganine hydrochloride (<strong>8</strong>)<strong>, </strong>lupinine (<strong>10</strong>) and cytisine (<strong>12</strong>) showed moderate inhibition with IC<sub>50</sub> values of 401.865 ±18.450, 863.322 ±6.415, 712.176±7.745, and 728.462±2.331<em> </em>µM, respectively. The results were compared with the standard antiglycation agent, rutin (<strong>13</strong>) (IC<sub>50 </sub>=98.012±2.030 µM).</p>Cellular cytotoxicity assay showed only gossypol acetic acid (<strong>2</strong>) and gossypolidene thiocarbamide (<strong>3</strong>) as somewhat toxic to 3T3 (mouse fibroblast) cells with IC<sub>50 </sub>values<em> </em>2.07 ±0.61 and 5.00 ±1.89 µM, respectively. Cycloheximide was used as a standard in this assay with IC<sub>50</sub> value 0.3 ± 0.089 μM

scholarly journals Inhibition of Fibroblast Apoptosis by Borrrelia afzelii, Coxiella burnetii and Bartonella henselae

2011 ◽  
Vol 60 (3) ◽  
pp. 269-272 ◽  
Author(s):  
TOMASZ CHMIELEWSKI ◽  
STANISŁAWA TYLEWSKA-WIERZBANOWSKA
Keyword(s):  
Cell Death ◽  
Chronic Disease ◽  
Coxiella Burnetii ◽  
Caspase 3 ◽  
Bartonella Henselae ◽  
Tissue Homeostasis ◽  
Host Cells ◽  
Fibroblast Cells ◽  
Human Fibroblast Cells ◽  
Long Time

Apoptosis is a genetically controlled mechanism of cell death involved in the regulation of tissue homeostasis. The aim of this study was to investigate the influence of Borrelia afzelii, Coxiella burnetii, and Bartonella henselae bacteria on apoptosis measured as the level of caspase 3 activity in human fibroblast cells HEL-299. Our findings show that C. burnetii bacteria may inhibit the process of apoptosis in the host cells for a long time. This can permit intracellular survival in the host and mediatingthe development of chronic disease.

Effect of Surface Charge on the Uptake of Magnetic Nanoparticles in Mouse Fibroblast Cells

2017 ◽  
Vol 8 ◽  
pp. 1-5 ◽  
Author(s):  
Jefunnie S. Matahum ◽  
Chao-Ming Su ◽  
Wei-Jie Wang ◽  
Shyh-Liang Lou ◽  
Tzong-Rong Ger
Keyword(s):  
Magnetic Nanoparticles ◽  
Surface Charge ◽  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
Effect Of Surface

Studies Of The Effect Of Temperature Shocks On Preparation For Cell Division In Mouse Fibroblast Cells (L Cells)

1973 ◽  
Vol 13 (3) ◽  
pp. 889-900
Author(s):  
HIROSHI MIYAMOTO ◽  
L. RASMUSSEN ◽  
E. ZEUTHEN
Keyword(s):  
Cell Division ◽  
Generation Time ◽  
Temperature Treatment ◽  
Mouse Fibroblast ◽  
Effect Of Temperature ◽  
Fibroblast Cells ◽  
L Cells ◽  
Synchronous Cell ◽  
Heat Shocks ◽  
Partial Synchrony

As L cells go through their growth-division cycle they acquire the capacity to respond progressively more strongly to certain standard changes in the temperature of the environment. Using techniques described earlier, we found that chilling to 1, 6 or 10 °C for 1 h had little effect on the timing of the forthcoming division. Conversely, heating for 1 h to temperatures between 41 and 42 °C had a strong effect. Generally, the older the cell when heated, the more extended is its generation time; in other words, the longer is the forthcoming division postponed. We found evidence that late in the cycle the cells undergo transition from a state in which they are maximally delayed with respect to the performance of a division to one in which they are less delayed. We attempted to synchronize cell divisions with single and with series of heat shocks (41.6 °C for 1 h). Like our predecessors in the field, we obtained only partial synchrony. However, because L cells appear to prepare for division between shocks, and because heat shocks tend to reverse such preparations for division, we find reason to continue these experiments, using previous experience with Tetrahymena and Schizosaccharomyces as a guide. Both the latter cells respond to proper temperature treatment with synchronous cell division.

The Uptake of Vitamins by Mouse Fibroblast Cells (Strain LS) During Growth in a Chemically Defined Medium

1971 ◽  
Vol 8 (3) ◽  
pp. 701-708
Author(s):  
G. J. BLAKER ◽  
S. J. PIRT
Keyword(s):  
Folic Acid ◽  
Cell Growth ◽  
Pantothenic Acid ◽  
Defined Medium ◽  
Mouse Fibroblast ◽  
Growth Yields ◽  
Fibroblast Cells ◽  
Chemically Defined Medium ◽  
Assay Methods ◽  
Early Phases

The uptake of biotin, choline, folic acid, hypoxanthine, inositol, nicotinamide, pantothenic acid, pyridoxine, riboflavin, thiamine and vitamin B12 by mouse LS cells in suspension culture was determined by microbiological assay methods. Based on the extent of uptake during cell growth, vitamin growth yields (cells produced/unit mass of vitamin utilized) were estimated for all of the vitamins, except folic acid, thiamine and B12. The growth yields were lower during the early phases of culture. No uptakes of folic acid or B12 could be demonstrated. During the period of incubation about half of the thiamine was irretrievably lost through spontaneous decomposition.

DIFFERENCES IN CYTOCOMPATIBILITY BETWEEN MWCNTs AND CARBOXYLIC FUNCTIONALIZED MWCNTs

2013 ◽  
Vol 06 (01) ◽  
pp. 1250053 ◽  
Author(s):  
YITENG ZHANG ◽  
DEJUN LI ◽  
MENGLI ZHAO ◽  
MEIXIAN GUO ◽  
XIANGYUN DENG ◽  
...  
Keyword(s):  
Contact Angle ◽  
Umbilical Vein ◽  
Mouse Fibroblast ◽  
Carboxyl Groups ◽  
Fibroblast Cells ◽  
Functional Surface ◽  
Multiwalled Carbon ◽  
Water Contact ◽  
Umbilical Vein Endothelial Cells ◽  
Functionalized Mwcnts

Influence of carboxylic functionalization on the cytocompatibility of multiwalled carbon nanotubes (MWCNTs) was investigated in this work. Water contact angle assay showed that the surface of MWCNTs-containing carboxyl (MWCNTs-COOH) became much more hydrophilic compared with pure MWCNTs. In cell-adhesion assays, two cell lines, mouse fibroblast cells (L929) and human umbilical vein endothelial cells (EAHY926) were used to assess the cytocompatibility of materials. The MWCNTs-COOH displayed the improved cell proliferation, viability and adhesion due to the enhanced wettability, indicating their superior cytocompatibility over MWCNTs. The existence of carboxyl groups should be benefit to the adhesion and growth of both cells, which implied that MWCNTs-COOH were helpful for seeding both cells and could be used as the functional surface for the adhesion and growth of cells.

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