Cancer treatments using low-temperature plasma

2021 ◽  
Vol 28 ◽  
Author(s):  
Hiromasa Tanaka ◽  
Masaaki Mizuno ◽  
Kenji Ishikawa ◽  
Shinya Toyokuni ◽  
Hiroaki Kajiyama ◽  
...  
Keyword(s):  
Low Temperature ◽  
Signaling Pathways ◽  
Medical Application ◽  
Low Temperature Plasma ◽  
Key Factors ◽  
Temperature Plasma ◽  
In Vivo Experiments ◽  
Stress Dependent

: Low-temperature plasma (LTP) is a partially ionized gas that contains electrons, ions, radicals, light, etc. Recently, the bio-medical application of LTP has become a hot topic in plasma science and biological science. Cancer treatment with plasma is the most challenging topic in plasma bio-medical applications. Many in vitro and in vivo experiments have been conducted to investigate the anti-tumor effects of LTP. Extracellular reactive oxygen and nitrogen species (RONS) in plasma-activated solutions are key factors for the anti-tumor effects, and amino acid modifications by LTP may affect cellular responses. Intracellular RONS are also key factors for the anti-tumor effects. Various signaling pathways such as p53 signaling pathways, survival and proliferation signaling pathways, and oxidative stress-dependent signaling pathways are activated by LTP.

In vivo monitoring of nicotine biosynthesis in tobacco leaves by low-temperature plasma mass spectrometry

Talanta ◽  
2018 ◽  
Vol 185 ◽  
pp. 324-327 ◽  
Author(s):  
Sandra Martínez-Jarquín ◽  
Humberto Herrera-Ubaldo ◽  
Stefan de Folter ◽  
Robert Winkler
Keyword(s):  
Mass Spectrometry ◽  
Low Temperature ◽  
Low Temperature Plasma ◽  
Temperature Plasma ◽  
Tobacco Leaves ◽  
In Vivo Monitoring ◽  
Plasma Mass Spectrometry ◽  
Nicotine Biosynthesis

scholarly journals Interrupting Neuron—Tumor Interactions to Overcome Treatment Resistance

Cancers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3741
Author(s):  
Patrick J. Hunt ◽  
Katherine E. Kabotyanski ◽  
George A. Calin ◽  
Tongxin Xie ◽  
Jeffrey N. Myers ◽  
...  
Keyword(s):  
Growth Factors ◽  
Tumor Microenvironment ◽  
Signaling Pathways ◽  
Treatment Strategies ◽  
Treatment Resistance ◽  
Trophic Relationships ◽  
Surgical Interventions ◽  
In Vivo Experiments

Neurons in the tumor microenvironment release neurotransmitters, neuroligins, chemokines, soluble growth factors, and membrane-bound growth factors that solid tumors leverage to drive their own survival and spread. Tumors express nerve-specific growth factors and microRNAs that support local neurons and guide neuronal growth into tumors. The development of feed-forward relationships between tumors and neurons allows tumors to use the perineural space as a sanctuary from therapy. Tumor denervation slows tumor growth in animal models, demonstrating the innervation dependence of growing tumors. Further in vitro and in vivo experiments have identified many of the secreted signaling molecules (e.g., acetylcholine, nerve growth factor) that are passed between neurons and cancer cells, as well as the major signaling pathways (e.g., MAPK/EGFR) involved in these trophic interactions. The molecules involved in these signaling pathways serve as potential biomarkers of disease. Additionally, new treatment strategies focus on using small molecules, receptor agonists, nerve-specific toxins, and surgical interventions to target tumors, neurons, and immune cells of the tumor microenvironment, thereby severing the interactions between tumors and surrounding neurons. This article discusses the mechanisms underlying the trophic relationships formed between neurons and tumors and explores the emerging therapies stemming from this work.

Effect Of Low-Temperature Plasma On Metastatic Processes Of Solid Tumors In Vitro

2018 ◽  
Vol 9 ◽  
pp. 22-23
Author(s):  
Angela Privat-Maldonado ◽  
Evelien Smits ◽  
Annemie Bogaerts
Keyword(s):  
Low Temperature ◽  
Solid Tumors ◽  
Low Temperature Plasma ◽  
Temperature Plasma

scholarly journals Src acts as the target of matrine to inhibit the proliferation of cancer cells by regulating phosphorylation signaling pathways

2020 ◽  
Author(s):  
Xi Zhang ◽  
Hui Xu ◽  
Xiaoyang Bi ◽  
Guoqing Hou ◽  
Andong Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cancer Cells ◽  
Signaling Pathways ◽  
Kinase Activity ◽  
Src Kinase ◽  
Kinase Domain ◽  
Akt Phosphorylation ◽  
In Vivo Experiments

Background and Purpose: Identification of accurate targets is essential for a successful development of targeted therapy in cancer. Studies have shown that matrine has antitumor activity against many types of cancers. However, the direct target in cancer cells of its anticancer effect has not been identified. The purpose of this study was to find the molecular target of matrine to inhibit the proliferation of cancer cells and explore its mechanism of action. Experimental Approach: The effect of matrine on the proliferation of cancer cells were examined by MTT assay. Pull-down assay and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) were performed to explore the target of matrine. A series of in vitro and in vivo experiments were conducted to reveal the mechanisms by which matrine targeted Src to regulate the downstream signaling pathways of Src in cancer cells. Key Results: Herein we showed that matrine inhibited the proliferation of cancer in vitro and in vivo. Pull-down assay with matrine-amino coupling resins (MA beads) and LC-MS/MS identified Src as the target of matrine. Src kinase domain is required for its interaction with matrine and Ala392 in the kinase domain participated in matrine-Src interaction. Intriguingly, matrine was proven to inhibit Src kinase activity in a non-ATP-competitive manner by blocking the autophosphorylation of Tyr419. Matrine down-regulated the phosphorylation levels of MAPK/ERK, JAK2/STAT3 and PI3K/Akt signaling pathways. Conclusions and Implications: Collectively, matrine targeted Src, inhibited kinase activity and down-regulated its downstream MAPK/ERK, JAK2/STAT3 and PI3K/Akt phosphorylation signaling pathways to inhibit the proliferation of cancer cells.

Bactericidal effect of low-temperature plasma against Helicobacter pylori in vitro

2019 ◽  
Vol 163 (3) ◽  
pp. 51-57
Author(s):  
V.G. Zhukhovitsky ◽  
◽  
M.V. Kazakova ◽  
E.V. Sysolyatina ◽  
R.A. Loleit ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Low Temperature ◽  
Bactericidal Effect ◽  
Low Temperature Plasma ◽  
Temperature Plasma

scholarly journals Src Acts as the Target of Matrine to Inhibit the Proliferation of Cancer Cells by Regulating Phosphorylation Signaling Pathways

2020 ◽  
Author(s):  
Xi Zhang ◽  
Hui Xu ◽  
Xiaoyang Bi ◽  
Guoqing Hou ◽  
Andong Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cancer Cells ◽  
Signaling Pathways ◽  
Kinase Activity ◽  
Src Kinase ◽  
Kinase Domain ◽  
Competitive Binding Assay ◽  
In Vivo Experiments

Abstract Background: Identification of accurate targets is essential for a successful development of targeted therapy in cancer. Studies have shown that matrine has antitumor activity against many types of cancers, including lung cancer, breast cancer, liver cancer, pancreatic cancer, ovarian cancer and leukemia, etc. However, the direct target in cancer cells of its anticancer effect has not been identified. The purpose of this study was to find the molecular target of matrine to inhibit the proliferation of cancer cells and explore its mechanism of action. Methods: The effect of matrine on the proliferation of cancer cells were examined by MTT assay. Pull-down assay with matrine-amino coupling resins (MA beads) and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) were performed to explore the target of matrine. The target of matrine was further validated by competitive binding assay, cellular thermal shift assay (CETSA) and drug affinity responsive target stability (DARTS). A series of in vitro and in vivo experiments were conducted to reveal the mechanisms by which matrine targeted Src to regulate the downstream signaling pathways of Src in cancer cells. Results: Herein we showed that matrine inhibited the proliferation of cancer in vitro and in vivo. Pull-down assay with MA beads and LC-MS/MS identified Src as the target of matrine. The findings provided solid evidences that matrine directly bound to Src and Src kinase domain is required for its interaction with matrine and Ala392 in the kinase domain participated in matrine-Src interaction. Intriguingly, matrine was proven to inhibit Src kinase activity in a non-ATP-competitive manner by blocking the autophosphorylation of Tyr419 in Src kinase domain. Matrine down-regulated the phosphorylation levels of MAPK/ERK, JAK2/STAT3 and PI3K/Akt signaling pathways via targeting Src.Conclusions: Collectively, matrine targeted Src, inhibited its kinase activity and down-regulated its downstream MAPK/ERK, JAK2/STAT3 and PI3K/Akt phosphorylation signaling pathways to inhibit the proliferation of cancer cells.

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