A Review of Analytical Methods for the Determination of Hypericin in Foods, Herbal, Biological and Pharmaceutical Matrices

2020 ◽  
Vol 26 (36) ◽  
pp. 4648-4657
Author(s):  
Patricia Rocha de Araújo ◽  
Bruno Fonseca-Santos ◽  
Ana Carolina Kogawa ◽  
Hérida Regina Nunes Salgado ◽  
Marlus Chorilli
Keyword(s):  
Physicochemical Properties ◽  
Analytical Methods ◽  
Pharmaceutical Preparations ◽  
Antidepressant Activity ◽  
Biological Properties ◽  
Alkaline Solutions ◽  
Ultraviolet Region ◽  
Organic Bases ◽  
Layer Chromatography

Aims: A review of analytical methods for the determination of hypericin in foods, herbal, biological and pharmaceutical matrices. Background: Hypericin (HYP) is a naturally-occurring pigment obtained from some plants of the genus Hypericum. Although HYP has been known for many years, it has recently attracted attention due to its varied biological properties, such as anti-inflammatory and antidepressant activity and it is also an efficient photosensitizer. Objective: The objective of this review is to provide insights into the physicochemical properties of HYP, as well as to report the analytical methods existing in the literature and official compendia for different matrices. Methods: The survey data were collected by Google Scholar® and Scopus® using keys terms. Result: Analytical methods involving HYP are mainly concerned with the quality control of pharmaceutical preparations, foods, beverages, biological samples and drug delivery systems using different types of analysis methods. Some difficulties have also been identified due to the physicochemical properties of HYP. It presents great solubility in alkaline solutions, organic bases and common polar organic solvents. Conclusion: It can be analyzed by thin layer chromatography, spectrophotometry in the ultraviolet region, but the most commonly used method is by HPLC. HYP presents monographs in the American, British and European Pharmacopoeias, however, the methods of analysis are not yet harmonized.

scholarly journals A review of analytical methods for the determination of four new phosphodiesterase type 5 inhibitors in biological samples and pharmaceutical preparations

2013 ◽  
Vol 49 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Cristiane Franco Codevilla ◽  
Tamara dos Santos Castilhos ◽  
Ana Maria Bergold
Keyword(s):  
Erectile Dysfunction ◽  
Analytical Methods ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Sexual Problem ◽  
Phosphodiesterase Type 5 Inhibitors ◽  
Phosphodiesterase Type 5 Inhibitor ◽  
Phosphodiesterase Type

The introduction of oral phosphodiesterase type 5 inhibitor therapy in 1998 revolutionized the treatment of erectile dysfunction. Erectile dysfunction is the most common sexual problem in men. It often has a profound effect on intimate relationships and quality of life. The analysis of pharmaceuticals is an important part of the drug development process as well as for routine analysis and quality control of commercial formulations. Whereas the determination of sildenafil citrate, vardenafil and tadalafil are well documented by a variety of methods, there are few publications about the determination of udenafil, lodenafil carbonate, mirodenafil and avanafil. The paper presents a brief review of the action mechanism, adverse effects, pharmacokinetics and the most recent analytical methods that can determine drug concentration in biological matrices and pharmaceutical formulations of these four drugs.

scholarly journals Review for Analytical Methods for the Determination of Mefenamic Acid

2021 ◽  
pp. 426-437
Author(s):  
T. V. Ashira ◽  
T. Mohamed Rafeeque ◽  
Saba Maanvizhi ◽  
S. Kathirvel ◽  
P. C. Indukala ◽  
...  
Keyword(s):  
Mefenamic Acid ◽  
Analytical Methods ◽  
High Performance ◽  
Active Pharmaceutical Ingredient ◽  
Review Article ◽  
Computer Assisted ◽  
Extensive Literature ◽  
Pharmaceutical Dosage Forms ◽  
Layer Chromatography

Mefenamic acid (MFA) is a non-steroidal anti-inflammatory drug that belongs to the anthranilic acid derivative family. It is used to relieve mild to moderate pain. The present review article includes a compilation of articles on the various properties along with an extensive literature survey on the reported analytical methods of MFA. Using a comprehensive computer assisted literature review; this article discusses the analytical methodologies for quantifying MFA both in active pharmaceutical ingredient and pharmaceutical dosage forms. This is the first review article in this series with focus on the analytical profile of MFA. Although, several methods like High Performance Liquid Chromatography (HPLC), Thin Layer Chromatography (TLC), spectrophotometry, fluorimetry, turbidimetry, Atomic Absorption Spectroscopy (AAS), Mass Spectroscopy (MS) and electro analytical methods were reported in the literature, HPLC stands out first for the quantification of MFA.

scholarly journals Recent Trends in the Application of Chromatographic Techniques in the Analysis of Luteolin and Its Derivatives

Biomolecules ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 731 ◽  
Author(s):  
Juszczak ◽  
Zovko-Končić ◽  
Tomczyk
Keyword(s):  
Liquid Chromatography ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Analytical Methods ◽  
High Performance ◽  
Biological Effects ◽  
Immunomodulatory Activity ◽  
Layer Chromatography

Luteolin is a flavonoid often found in various medicinal plants that exhibits multiple biological effects such as antioxidant, anti-inflammatory and immunomodulatory activity. Commercially available medicinal plants and their preparations containing luteolin are often used in the treatment of hypertension, inflammatory diseases, and even cancer. However, to establish the quality of such preparations, appropriate analytical methods should be used. Therefore, the present paper provides the first comprehensive review of the current analytical methods that were developed and validated for the quantitative determination of luteolin and its C- and O-derivatives including orientin, isoorientin, luteolin 7-O-glucoside and others. It provides a systematic overview of chromatographic analytical techniques including thin layer chromatography (TLC), high performance thin layer chromatography (HPTLC), liquid chromatography (LC), high performance liquid chromatography (HPLC), gas chromatography (GC) and counter-current chromatography (CCC), as well as the conditions used in the determination of luteolin and its derivatives in plant material.

scholarly journals High-Performance Thin-Layer Chromatography and Three-Dimensional Image Analysis for the Determination of Rutin in Pharmaceutical Preparations

2010 ◽  
Vol 93 (3) ◽  
pp. 804-810 ◽  
Author(s):  
Florin Soponar ◽  
Augustin C MoŢ ◽  
Costel Sârbu
Keyword(s):  
High Performance ◽  
Visual Detection ◽  
Three Dimensional ◽  
Pharmaceutical Preparations ◽  
Digital Processing ◽  
Amino Groups ◽  
Flatbed Scanner ◽  
Layer Chromatography ◽  
Hptlc Method

Abstract A novel HPTLC method was developed for fast and simple quantitative determination of rutin in pharmaceutical preparations. The proposed method combines the advantages of HPTLC with the comfort and the convenience of digital processing of images. For the separation of rutin, silica gel with attached amino groups was used as the stationary phase and ethyl acetateformic acidmethanolwater (10 + 0.9 + 1.1 + 1.7, v/v/v/v) as the mobile phase. The chromatographic plate was sprayed with 2-aminoethyldiphenyl borate solution for visual detection of the spots. For the construction of a three-dimensional chromatogram, Melanie 7.0 software was used together with an HP flatbed scanner that allowed capture of the images on chromatographic plates. The calibration curve was linear within the range of 0.954.78 g/spot with an r value of 0.9984. The RSD for six replicates at three concentration levels was less than 3, while the recovery was between 97.28 and 103.27. The proposed method was found to be simple, precise, sensitive, and accurate and has been applied for the determination of rutin in two commercial drugs. The results were compared with the results of other techniques that generate bidimensional chromatograms and validated by UV-Vis spectrophotometry.

scholarly journals Analytical Methods for Determination of Muscle Relax-ant Thiocolchicoside in Pharmaceutical Preparations- A Review

2015 ◽  
Vol 2 (1) ◽  
pp. 43-55 ◽  
Author(s):  
J.K. Rajput ◽  
P.H. Patil ◽  
S. J. Surana ◽  
A. A. Shirkhedkar
Keyword(s):  
Analytical Methods ◽  
Bulk Material ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Low Back ◽  
Drug Substances ◽  
Uv Visible ◽  
Immune Assays ◽  
Acting Muscle

Thiocolchicoside is a centrally acting muscle relaxant and used in combination with many NSAIDs for the treatment of various musculoskeletal disorders. As it is less sedative than other centrally acting muscle relaxants hence frequently prescribed for low back pain (LBP), orthopedic, traumatic and rheumatologic disorders. It is available in market in single component and as multicomponent formulations. Various analytical methods are available for determination of thiocolchico-side in drug substances and formulated products. The present article summarizes more than 100 analytical methods including all types of chromatographic, UV-Visible spectrophotometry and radio immune assays with their percentage of utility for de-termination of thiocolchicoside in biological matrices, bulk material and different pharmaceutical formulations.

scholarly journals MITRAGYNINE PERCENTAGES OF VARIOUS KRATOM VARIANTS SEIZED IN INDONESIA: A QUANTITATIVE ANALYSIS USING LIQUID CHROMATOGRAPHY-PHOTO DIODE ARRAY DETECTOR

2021 ◽  
pp. 252-256
Author(s):  
TANTI ◽  
CHRISTIEN ANDRIYANI LALANGI ◽  
ERI ARFIYANI ◽  
WIDIANTI NINGTIAS ◽  
ERLANA NINDYA MAULIDA
Keyword(s):  
Liquid Chromatography ◽  
Analytical Methods ◽  
Uv Light ◽  
Gradient Elution ◽  
Array Detector ◽  
Mobile Phases ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Layer Chromatography

Objective: This study details the determination of mitragynine in various kratom samples using the thin-layer chromatography (TLC) technique and validation of analytical methods for quantifying the concentration of mitragynine in various kratom samples using liquid chromatography with photodiode array detector (LC-PDA). Methods: TLC technique using n-hexane: ethyl acetate: ammonia 25% (30: 15: 1 v/v/v) was applied to isolate mitragynine from kratom samples. Several interesting spots obtained were visualized under UV light at 254 nm. Samples were also prepared with organic solvent extraction directly prior to LC analysis (non-isolation method of preparation) to quantify the concentration of mitragynine. Mobile phases used were acetonitrile (MP A) and 0.1% formic acid in water (MP B). Samples and standards were run by gradient elution with a flow rate of 0.3 ml/min, detection using PDA detector at 254 nm. Results: Mitragynine was successfully isolated from kratom samples in Rf 0.50 by TLC system applied. The validation of analytical methods for mitragynine passed the acceptance criteria as described by UNODC Guidance. The concentration of mitragynine in various kratom samples seized in Indonesia ranged from 0.37%-1.70% (%w/w). Conclusion: Both TLC and LC analytical methods could be applied to determine and quantify the concentration of mitragynine in each examined sample, respectively.

Sign in / Sign up

Export Citation Format

Share Document