Development of Coffee Reference Material for Coffea arabica and C. canephora Blends

SummaryIn a collaborative trial of eleven laboratories which was performed mainly within the framework of the European Community Bureau of Reference (BCR), a second reference material for thromboplastin, rabbit, plain, was calibrated against its predecessor RBT/79. This second reference material (coded CRM 149R) has a mean International Sensitivity Index (ISI) of 1.343 with a standard error of the mean of 0.035. The standard error of the ISI was determined by combination of the standard errors of the ISI of RBT/79 and the slope of the calibration line in this trial.The BCR reference material for thromboplastin, human, plain (coded BCT/099) was also included in this trial for assessment of the long-term stability of the relationship with RBT/79. The results indicated that this relationship has not changed over a period of 8 years. The interlaboratory variation of the slope of the relationship between CRM 149R and RBT/79 was significantly lower than the variation of the slope of the relationship between BCT/099 and RBT/79. In addition to the manual technique, a semi-automatic coagulometer according to Schnitger & Gross was used to determine prothrombin times with CRM 149R. The mean ISI of CRM 149R was not affected by replacement of the manual technique by this particular coagulometer.Two lyophilized plasmas were included in this trial. The mean slope of relationship between RBT/79 and CRM 149R based on the two lyophilized plasmas was the same as the corresponding slope based on fresh plasmas. Tlowever, the mean slope of relationship between RBT/79 and BCT/099 based on the two lyophilized plasmas was 4.9% higher than the mean slope based on fresh plasmas. Thus, the use of these lyophilized plasmas induced a small but significant bias in the slope of relationship between these thromboplastins of different species.

Download Full-text

Effect of Concentration of Trisodium Citrate Anticoagulant on Calculation of the International Normalised Ratio and the International Sensitivity Index of Thromboplastin

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648816 ◽

1994 ◽

Vol 72 (01) ◽

pp. 084-088 ◽

Cited By ~ 29

Author(s):

E M Duncan ◽

C R Casey ◽

B M Duncan ◽

J V Lloyd

Keyword(s):

Reference Material ◽

Oral Anticoagulants ◽

Reference Method ◽

Trisodium Citrate ◽

Sensitivity Index ◽

Blood Samples ◽

Orthogonal Regression ◽

Significant Difference ◽

International Normalised Ratio ◽

International Sensitivity Index

SummaryThe aim of this study was to determine whether the concentration of trisodium citrate used to anticoagulate blood has an effect on the INR of the sample and the ISI of the thromboplastin. Five thromboplastins including and Australian reference material were used to measure the prothrombin time of normal and patient samples collected into two concentrations of trisodium citrate - 109 mM and 129 mM. There was no effect of citrate concentration on the INRs determined with the reference material. However for the other four thromboplastins there was a significant difference between INRs for the two citrate groups. The prothrombin times of the samples collected into 129 mM were longer than those collected into 109 mM. This difference was only slight in normal plasma but more marked in patients receiving oral anticoagulants, causing the INRs for patient plasmas collected into 129 mM citrate to be higher then the corresponding samples collected into 109 mM citrate.From orthogonal regression of log prothrombin times by the reference method against each thromboplastin, we found that the ISI for each thromboplastin was approximately 10% lower when determined with samples collected into 129 mM citrate than with samples collected into 109 mM. These results suggest that the concentration of trisodium citrate used for collection of blood samples can affect the calculation of the INR and the calibration of the ISI of thromboplastin. This was found both for commercial thromboplastins prepared by tissue extraction and for a recombinant tissue factor.

Download Full-text

SISTEMA DE PRODUCCIÓN DE CAFÉ (Coffea arabica L.) EN LA COMUNIDAD DEL CERRO CUATE, ILIATENCO, GUERRERO

RECUBRIMIENTO COMESTIBLE CON BASE DE PIÑON MEXICANO (Jatropha curcas) SOBRE LA CALIDAD DE CHAYOTE MINIMAMENTE PROCESADO ◽

10.32854/agrop.v11i10.1262 ◽

2018 ◽

Vol 11 (10) ◽

Author(s):

A. Delgado-Alvarado

Keyword(s):

Coffea Arabica ◽

El Sistema ◽

Coffea Arabica L

Objetivo: Analizar el proceso de producción y comercialización de café en la comunidad del Cerro Cuate, Iliatenco, en la región de la Montaña de Guerrero, México.Diseño/Metodología/aproximación: el trabajo se realizó por; 1) investigación documental), 2) selección del área de estudio, 3) Entrevista estructurada con preguntas abiertas a 22 cafeticultores, soportada con la técnica de encuesta seccional y la herramienta de cédulas de entrevistas, y 4) análisis de la información. El tamaño de muestra se definió por el método de muestreo por conveniencia, y la selección de las unidades de análisis por la técnica bola de nieve. Resultados: El sistema de producción de café que predominó fue el sistema rústico de montaña, intercalado con plátano y frutales. La máxima productividad de las plantaciones se alcanza de 5.5 a 7.5años de edad, la renovación de plantas la hacen a los 12 años. El rendimiento de café fue de 3.7 kg por planta por año. La producción del café la realizan principalmente hombres (77.3%), entre 56 y 70años de edad. La venta se realiza en la presentación de café capulín a granel a intermediarios, a la ARIC, a CAFECO, a la Unión de Ejidos y a la Organización Mixtrui.Limitaciones del estudio/implicaciones: Se da una propuesta de mejora para favorecer el proceso de producción, beneficio y comercialización del café.Hallazgos/conclusiones: Coffea arabica es el principal café que se cultiva, con las variedades Typica, Caturra, Mundo Novo, Garnica y Bourbón. Los factores que limitan su producción y calidad son faltade planeación en manejo del cultivo y no contar con asesoría técnica.

Download Full-text

Wake up and smell the coffee: an exploratory pre-pilot study assessing Coffea arabica solution to cover grey hair

Australian Journal of Herbal and Naturopathic Medicine ◽

10.33235/ajhnm.32.3.102-106 ◽

2020 ◽

Vol 32 (3) ◽

Author(s):

Freda Schoupinsky ◽

Xavier Schoupinsky

Keyword(s):

Pilot Study ◽

Coffea Arabica ◽

Grey Hair

Download Full-text

BIOASSAY CHARACTERISTICS OF INDIVIDUAL URINARY GONADOTROPHIN EXTRACTS

Acta Endocrinologica ◽

10.1530/acta.0.0420214 ◽

1963 ◽

Vol 42 (2) ◽

pp. 214-224

Author(s):

Raymond C. Mellinger ◽

Jalileh A. Mansour ◽

Richmond W. Smith

Keyword(s):

Reference Material ◽

Young Women ◽

Reference Standard ◽

General Acceptance ◽

Modifying Factors ◽

Large Numbers ◽

The World ◽

Quantitative Bioassay ◽

Different Parts ◽

Age And Sex

ABSTRACT A reference standard is widely sought for use in the quantitative bioassay of pituitary gonadotrophin recovered from urine. The biologic similarity of pooled urinary extracts obtained from large numbers of subjects, utilizing groups of different age and sex, preparing and assaying the materials by varying techniques in different parts of the world, has lead to a general acceptance of such preparations as international gonadotrophin reference standards. In the present study, however, the extract of pooled urine from a small number of young women is shown to produce a significantly different bioassay response from that of the reference materials. Gonadotrophins of individual subjects likewise varied from the multiple subject standards in many instances. The cause of these differences is thought to be due to the modifying influence of non-hormonal substances extracted from urine with the gonadotrophin and not necessarily to variations in the gonadotrophins themselves. Such modifying factors might have similar effects in a comparative assay of pooled extracts contributed by many subjects, but produce significant variations when material from individual subjects is compared. It is concluded that the expression of potency of a gonadotrophic extract in terms of pooled reference material to which it is not essentially similar may diminish rather than enhance the validity of the assay.

Download Full-text

ATIVIDADE DE AGUA, CRESCIMENTO MICROBIOLOGICO E PERDA DE MATÉRIA SECA DOS GRÃOS DE CAFÉ (Coffea arabica L.) EM DIFERENTES CONDIÇÕES DE ARMAZENAMENTO

Revista Brasileira de Produtos Agroindustriais ◽

10.15871/1517-8595/rbpa.v5n1p17-24 ◽

2003 ◽

Vol 5 (1) ◽

pp. 17-24 ◽

Cited By ~ 3

Author(s):

P.C. Afonso Júnior ◽

P.C. Corrêa ◽

F.S. Silva ◽

D.M. Ribeiro

Keyword(s):

Coffea Arabica ◽

Coffea Arabica L

Download Full-text

Deposição da calda de pulverização em diferentes volumes vegetativos de Coffea arabica L.

Coffee Science ◽

10.25186/cs.v12i1.1232 ◽

2017 ◽

Vol 12 (1) ◽

pp. 69 ◽

Cited By ~ 2

Author(s):

Felipe Santinato ◽

Renato Adriane Alves Ruas ◽

Carlos Diego Silva ◽

Rouverson Pereira Da Silva ◽

Victor Afonso Reis Gonçalves ◽

...

Keyword(s):

Coffea Arabica ◽

Para Determinar ◽

Coffea Arabica L

A disposição dos ramos e sobreposição das folhas em plantas de café dificultam a penetração da calda pulverizada. Portanto, para determinar o volume de calda adequado, é importante verificar o estado de enfolhamento da lavoura antes da aplicação. Objetivou-se com este trabalho, avaliar a deposição de caldas de pulverização em lavouras de café aplicadas em diferentes volumes vegetativos. Os tratamentos foram dispostos seguindo esquema de parcelas subdivididas em cada volume vegetativo (5.000; 7.500; 10.000 e 17.500 m3 ha-1). Sendo as parcelas cinco volumes de calda (150, 300, 450, 600 e 750 L ha-1) e as subparcelas três posições no dossel do cafeeiro (terço superior, médio e inferior) com quatro repetições. Nos quatro volumes vegetativos estudados, não ocorreu interação significativa (p>0,05) entre o volume aplicado e os diferentes volumes vegetativos. Porém, os volumes vegetativos de 5.000,0 e 17.500,0 m3 ha-1 a deposição foi crescente com aumento das vazões, o que pode ser atribuído a densidade foliar. Não foi verificada diferença estatística (p> 0,05), entre as médias de deposição nos diferentes terços (alturas) no dossel das plantas de café, nos volumes vegetativos 5.000,0 m3 ha-1, 10.000,0 m3 ha-1 e 17.500,0 m3 ha-1. Contudo, no volume de 7.500,0 m3 ha-1 houve maior deposição no terço mediano em relação ao terço inferior. A deposição é maior à medida que aumenta o volume de calda aplicado. Em plantas com menor densidade foliar, há incremento na deposição. A deposição é maior no terço mediano em relação ao terço inferior.

Download Full-text

Paclobutrazol e restrição hídrica no crescimento e desenvolvimento de plantas de café

Coffee Science ◽

10.25186/cs.v12i4.1361 ◽

2017 ◽

Vol 12 (4) ◽

pp. 534

Author(s):

André Felipe Fialho Ribeiro ◽

Sylvana Naomi Matsumoto ◽

Paula Acácia Silva Ramos ◽

Jefferson Luan Dias dos Santos ◽

Ednilson Carvalho Teixeira ◽

...

Keyword(s):

Coffea Arabica

O paclobutrazol pode atenuar os efeitos negativos do estresse hídrico em diversas espécies vegetais, todavia, para cafeeiros é necessário ampliar a base de conhecimentos sobre este tema. Neste estudo, objetivou-se avaliar a morfofisiologia de plantas jovens de Coffea arabica var. ‘Catuaí Vermelho IAC 144’ em resposta a diferentes regimes hídricos e concentrações de paclobutrazol aplicado via solo. O experimento foi conduzido em casa de vegetação em Vitória da Conquista, BA. O delineamento experimental foi em blocos casualizados, com esquema fatorial representado por cinco concentrações de paclobutrazol (0, 50, 100, 150 e 200 mg L-1) aplicado via solo e dois regimes hídricos (com e sem restrição hídrica), em cinco repetições. Aos 120 dias após a aplicação do regulador vegetal avaliou-se o status hídrico, o índice SPAD e as características morfológicas das plantas. A aplicação de paclobutrazol reduziu o índice SPAD e a área foliar individual, e favoreceu o status hídrico dos cafeeiros sob restrição hídrica. As plantas jovens de café, sem restrição hídrica e tratadas com o regulador vegetal, tiveram maior retenção foliar e índice SPAD, e menor área foliar individual. A aplicação de paclobutrazol em concentrações elevadas resultou em plantas mais compactas. A restrição hídrica proporcionou menor área foliar total, número de ramos e massa seca dos cafeeiros.

Download Full-text