Nutritional and Medicinal Importance of Dioscorea glabra R. Baron, a Potent Wild Edible Plant Consumed by the Lodha Tribal Community of West Bengal, India

2020 ◽  
Vol 16 (3) ◽  
pp. 284-295
Author(s):  
Sagari Chaudhury ◽  
Chowdhury Habibur Rahaman ◽  
Harish Singh ◽  
Kausik Chaudhuri ◽  
Tapan Seal
Keyword(s):  
Heavy Metals ◽  
Phenolic Acids ◽  
Hplc Analysis ◽  
Antioxidant Properties ◽  
Water Soluble ◽  
Total Phenolic ◽  
Tribal Community ◽  
Wild Edible Plant ◽  
Toxic Heavy Metals ◽  
Edible Plant

Objective: The present study designed to evaluate the proximate composition, minerals content and in vitro antioxidant properties, in the tuber of Dioscorea glabra R. Baron. The estimation of phenolic acids, polyphenolics and quantitation of water-soluble vitamins like ascorbic acid (C), thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6) and folic acid (B9) in the plant were carried out by High Performance liquid chromatography (HPLC) method. Methods: The proximate parameters, minerals and toxic heavy metals such as lead, cadmium, arsenic and mercury were estimated in the wild edible plant using standard food analysis techniques. The HPLC analysis was carried out using Dionex Ultimate 3000 liquid chromatograph attached with Acclaim C 18 column (5 μm particle size, 250 x 4.6 mm), and photo diode array (PDA) detector. Results: The present study showed the presence of a very good amount of protein (4.823±0.041%), carbohydrate (42.43±0.057%) and minerals and heavy metals in various amount. The total phenolic and flavonoid content were found 335.64 ± 3.92 mg/100gm and 65.73 mg/100gm, respectively in the plant. The plant showed the presence of a very good amount of vitamin C (23.49±0.0413 mg/100gm) along with water-soluble B vitamins ranged between 0.036 to 4.159 mg/100gm. The HPLC analysis also indicated the presence of phenolic acids and polyphenolics in various amounts in this wild edible plant. Conclusion: The abundance of protein, fat, carbohydrate, minerals, water soluble vitamins and natural antioxidant components in this plant makes them as a considerable source of nutrition and could be consumed as a regular diet to the human being.

scholarly journals Analysis of biochemical composition and antioxidant properties of nuts sold in the consumer marke

2020 ◽  
Vol 82 (2) ◽  
pp. 124-130
Author(s):  
L. P. Nilova ◽  
S. M. Malyutenkova
Keyword(s):  
Fatty Acids ◽  
Vitamin E ◽  
Phenolic Compounds ◽  
Biochemical Composition ◽  
Unsaturated Fatty Acids ◽  
Antioxidant Properties ◽  
Water Soluble ◽  
Total Phenolic ◽  
Consumer Market ◽  
Triton X

The results of studies of the biochemical composition and antioxidant properties of nuts sold in the consumer market of St. Petersburg were presented in the work. The objects of research were kernels of nuts: sweet almonds, hazelnuts, cashews, walnuts. Total Soxhlet lipids, fatty acid composition, vitamin E, fractional composition of tocopherols and phytosterols, total phenolic compounds and flavonoids, antioxidant activity by FRAP with ferric chloride, o-phenanthroline and Triton X 100 were determined in nuts. Nuts varied in lipid content (42.6–65.4%) with a predominance of unsaturated fatty acids from 80.4 to 92.4 relative%. Oleic acid prevailed in the fatty acids of almonds, hazelnuts and cashews, while linoleic acid prevailed in walnuts. Walnuts contained the highest amount of polyunsaturated fatty acids. The antioxidant properties of nuts were formed by a complex of water and fat-soluble antioxidants. Fat-soluble antioxidants included vitamin E with a predominance of ?-tocopherol in the kernels of sweet almonds and hazelnuts, ?-tocopherol - in walnuts and cashews. Only sweet almond kernels contained all the tocopherol fractions. ?- and ?-tocopherols were absent in hazelnut lipids, while ?-tocopherols were absent in cashews and walnuts. ?-sitosterol, campesterol and stigmasterol with a predominance of ?-sitosterol were identified in the composition of phytosterols in all nut kernels. Water-soluble antioxidants are mainly represented by phenolic compounds, the amount of which varies widely depending on the type of nuts: cashews

scholarly journals LC-ESI-QTOF/MS Characterisation of Phenolic Acids and Flavonoids in Polyphenol-Rich Fruits and Vegetables and Their Potential Antioxidant Activities

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 405 ◽  
Author(s):  
Chunhe Gu ◽  
Kate Howell ◽  
Frank R. Dunshea ◽  
Hafiz A. R. Suleria
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Acids ◽  
Fruits And Vegetables ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Antioxidant Power ◽  
Plant Foods

Polyphenols are naturally occurring compounds found largely in fruits and vegetables. The antioxidant properties of these polyphenols including total phenolic content (TPC), total flavonoid content (TFC), tannin content, 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH), 2,2′-azinobis-(3-ethylbenzo-thiazoline-6-sulfonic acid) (ABTS) scavenging abilities and ferric ion reducing antioxidant power (FRAP) were measured among sixteen (16) plant foods (mango, blueberry, strawberry, black carrot, raspberry, dark grapes, garlic, ginger, onion, cherry, plum, apple, papaya, peach, pear and apricot) by modifying, standardising and translating existing antioxidant methods using a 96-well plate reader. Eighteen targeted phenolic acids and flavonoids were characterised and quantified using high-performance liquid chromatography-photometric diode array (HPLC-PDA) and verified by modifying an existing method of liquid chromatography coupled with electrospray-ionisation triple quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF/MS). While most of these compounds were accurately detected by the HPLC-PDA at a low concentration, a few polyphenols in low concentrations could be only be characterised using the LC-ESI-QTOF/MS method. Our results showed that mango possessed the highest overall antioxidant activity, phenolic acid and flavonoid content among the selected fruits. Factor analysis (FA) and Pearson’s correlation tests showed high correlations among ABTS, DPPH, FRAP and phenolic acids, implying the comparable capabilities of scavenging the DPPH/ABTS free radicals and reducing ferric ions from the antioxidant compounds in the samples. Phenolic acids contributed significantly to the antioxidant activities, and flavonoids contributed more to tannin content based on the correlations. Overall, methods modified and standardized in this study can provide better understanding of high throughput technologies and increase the reliability of antioxidant data of different plant foods.

scholarly journals Allelopathic effects of Rhynchosia capitata on germination and seedling growth of mungbean

2013 ◽  
Vol 31 (3) ◽  
pp. 501-509 ◽  
Author(s):  
H.H. Ali ◽  
A. Tanveer ◽  
M.A. Nadeem ◽  
M.M. Javaid ◽  
M.S. Kashif ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Seedling Growth ◽  
Leaf Extract ◽  
Water Soluble ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Seedling Vigour ◽  
Water Extracts ◽  
Whole Plant ◽  
Vigour Index

Experiments were conducted to evaluate the allelopathic influence of Rhynchosia capitata on germination and seedling growth of mungbean (Vigna radiate) along with identification of the phytotoxic substances responsible for this activity. Water extracts of root, shoot, leaf, fruit and whole plant were prepared by soaking them in water in a ratio of 1:20 (w/v) for 24 h. All the extracts affected germination and seedling growth of mungbean, but higher inhibition was seen with R. capitata leaf water extracts. A linear decrease in the germination characteristics of mungbean was observed with the decrease in the concentration of leaf extract from 5% to 1%. The soil-incorporated residues (1-4% w/w) of R. capitata stimulated the growth of root and hypocotyl at low concentrations, while it inhibited their growth at higher concentrations. Rhynchosia capitata soil-incorporated residues (4% w/w) significantly reduced the seedling vigour index of mungbean in addition to their significant effect on total germination. A significant amount of water-soluble phenolic acids were found in R. capitata plant extracts. The content of total phenolic acids was higher in the leaf extract compared to that of the stem, fruit or root extracts. Two phenolic acids including vanillic acid and 4‑(hydroxymethyl) benzoic acid were found in R. capitata leaf extracts.

scholarly journals Identification of Phenolic Acids and Flavonoids in Monofloral Honey from Bangladesh by High Performance Liquid Chromatography: Determination of Antioxidant Capacity

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Mohammed Moniruzzaman ◽  
Chua Yung An ◽  
Pasupuleti Visweswara Rao ◽  
Mohammad Nurul Islam Hawlader ◽  
Siti Amirah Binti Mohd Azlan ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Acids ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Sugar Content ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
The Mean

The aim of the present study was to characterize the phenolic acids, flavonoids, and antioxidant properties of monofloral honey collected from five different districts in Bangladesh. A new high performance liquid chromatography (HPLC) equipped with a UV detector method was developed for the identification of the phenolic acids and flavonoids. A total of five different phenolic acids were identified, with the most abundant being caffeic acid, benzoic acid, gallic acid, followed by chlorogenic acid and trans-cinnamic acid. The flavonoids, kaempferol, and catechin were most abundant, followed by myricetin and naringenin. The mean moisture content, total sugar content, and color characteristics of the honey samples were 18.36 ± 0.95%, 67.40 ± 5.63 g/100 g, and 129.27 ± 34.66 mm Pfund, respectively. The mean total phenolic acids, total flavonoid content, and proline content were 199.20 ± 135.23, 46.73 ± 34.16, and 556.40 ± 376.86 mg/kg, respectively, while the mean FRAP values and DPPH radical scavenging activity were 327.30 ± 231.87 μM Fe (II)/100 g and 36.95 ± 20.53%, respectively. Among the different types of honey, kalijira exhibited the highest phenolics and antioxidant properties. Overall, our study confirms that all the investigated honey samples are good sources of phenolic acids and flavonoids with good antioxidant properties.

scholarly journals Micronized Oat Husk: Particle Size Distribution, Phenolic Acid Profile and Antioxidant Properties

Materials ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5443
Author(s):  
Dariusz Dziki ◽  
Wojciech Tarasiuk ◽  
Urszula Gawlik-Dziki
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Particle Size Distribution ◽  
Size Distribution ◽  
Phenolic Acids ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffraction Method ◽  
Total Phenolic ◽  
Oat Husk

Oat husk (OH; hull) is a by-product generated from oat processing and is rich in insoluble fibre and phenolic compounds. The aim of this work was to study the particle size distribution, antioxidant activity, and phenolic profile of micronized OH. For this purpose, the hull was first sterilized using superheated steam and was then ground using an impact classifier mill. The particle size distribution (PSD) of the ground husk was determined using the laser diffraction method and the parameters characterizing the PSD of the ground husk, and its antioxidant activity were calculated. In addition, UPLC-MS/MS analysis of phenolic acids was also performed. Micronization of the sterilized husk effectively decreased the size of the particles, and with the increasing speed of the rotor and classifier, the median size of the particles (d50) decreased from 63.8 to 16.7 µm. The following phenolic acids were identified in OH: ferulic, caffeic, p-hydroxybenzoic, vanillic, syringic, and synapic acid. Ferulic acid constituted about 95% of total phenolic acids. The antioxidant activity of the obtained extracts increased as the particle size of the micronized husk decreased. The highest half maximal inhibitory concentration (EC50 index) was found for chelating power, and the lowest was found in the case of radical scavenging activity against DPPH.

scholarly journals Phenolic acids and antioxidant properties of barley wholegrain and pearling fractions

2012 ◽  
Vol 21 (2) ◽  
pp. 118-131 ◽  
Author(s):  
Tamer Gamel ◽  
El-Sayed M Abdel-Aal
Keyword(s):  
Phenolic Acids ◽  
Functional Foods ◽  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Ldl Oxidation ◽  
Total Phenolic ◽  
Inhibitory Effects ◽  
Scavenging Capacity ◽  
Wide Range

Demand for functional foods that offer therapeutic and disease-prevention properties is globally increasing. Barley presents an opportunity in this regard due to its high antioxidants and dietary fiber contents. Barley wholegrain and milling fractions were investigated in terms of phenolic acids composition, scavenging capacity against DPPH and ABTS radicals and inhibition of human LDL oxidation in vitro. Ferulic and p-coumaric were the primary phenolic acids in wholegrain and pearled fractions accounting for 43-97% and 2-55% of total phenolic acids, respectively. This wide range demonstrates significant differences among barley wholegrain or pearled products. Antioxidant capacities also varied significantly among barleys with the hulless cultivars tested exhibiting the greatest. The outer layers fraction showed higher scavenging capacity against ABTS and DPPH radicals and inhibitory effects toward LDL oxidation compared with the endosperm fractions. The results suggest that the outer layer and hull fractions would potentially provide promising sources of natural antioxidants.

scholarly journals KOMPOSISI SENYAWA FENOL DAN POTENSI ANTIOKSIDAN DARI KACANG-KACANGAN: REVIEW

2020 ◽  
Vol 14 (01) ◽  
pp. 91
Author(s):  
Nurud Diniyah ◽  
Sang-Han Lee
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Seed Coat ◽  
Water Soluble ◽  
Grain Legume ◽  
Total Phenolic ◽  
Hydroxyl Groups ◽  
Chemical Structures ◽  
Legume Seeds

Legumes are good dietary source of bioactive phenolic compounds which play significant roles in many physiological as well as metabolic processes. Flavonoids, phenolic acids and condensed tannins are the main phenolic compounds that are provide in legume seeds. Majority of the phenolic compounds are serving in the legume seed coats. The majority of seed coat of legume seeds are phenolic acids and flavonoids (mainly catechins and procyanidins). Gallic and protocatechuic acids are common in kidney bean and mung bean. Almost 70% of total phenolic compounds in lentils and cranberry beans (seed coat) are catechins and procyanidins. The antioxidant activity of phenolic compounds is in lineal intercourse with their chemical structures such as number as well as position of the hydroxyl groups. Processing mostly conducts to the alleviation of phenolic compounds in legumes because of chemical rearrangements. Phenolic content also lessen due to leaching of water-soluble phenolic compounds into the cooking water. This review provides comprehensive information of phenolic compounds identified in grain legume seeds along with discussing their antioxidant. Keywords: antioxidant activity, legume seeds, phenolic compounds, processing

scholarly journals THE COMPARATIVE STUDY OF ANTIOXIDANT PROPERTIES OF SOME SAMBUCUS L. SPECIES

2019 ◽  
pp. 127-137 ◽  
Author(s):  
Любовь (Lyubov') Николаевна (Nikolaevna) Скрыпник (Skrypnik) ◽  
Алина (Alina) Андреевна (Andreevna) Курашова (Kurashova)
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Total Content ◽  
Water Soluble ◽  
Biologically Active ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Sambucus Ebulus ◽  
Amperometric Method

The antioxidant properties of fruits, flowers, leaves, bark (or stem) of elderberry (Sambucus nigra L.), red elderberry (Sambucus racemosa L.) and dwarf elder (Sambucus ebulus L.) were investigated. The total content of anthocyanins and total phenolic compounds content by using of Folin-Ciocalteu assay were determined spectrophotometrically. The total content of water-soluble antioxidants was investigated by amperometric method. The antioxidant activity (AOA) of plant extracts was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2’azinobis(3)ethylbenzthiazoline-6- sulfonic acid) radical and FRAP (ferric reducing antioxidant power) assays. It was established that the fruits of plants of the genus Sambucus L. were characterized by the maximum level of all studied antioxidants. High content of anthocyanins and phenolic compounds was determined in the leaves of elderberry and dwarf elder. The flowers of these elderberry species were distinguished by a high total content of water-soluble antioxidants. The highest antioxidant activity was observed in fruits extracts in comparison with other parts of the plant. Higher antioxidant activity was identified in the extract of the fruits of elderberry and dwarf elder than of the red elderberry fruits. The most optimal method for evaluating the antioxidant activity of elder extracts was the FRAP assay, which showed the highest correlation between AOA and individual antioxidant components, compared to DPPH and ABTS assays. Comparative analysis of antioxidant content and antioxidant activity of various plant parts of three elderberry species showed that the most promising sources of biologically active substances with antioxidant properties are fruits and flowers of elderberry and dwarf elder.

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