A Novel Non-invasive Effective Method for Potential Treatment of Degenerative Disc Disease: A Hypothesis

2019 ◽  
Vol 19 (1) ◽  
pp. 8-14
Author(s):  
Siva G. Somasundaram ◽  
Cristian Muresanu ◽  
Pamela Schield ◽  
Alfiya Makhmutovа ◽  
Elena V. Bovina ◽  
...  
Keyword(s):  
Transforming Growth Factor ◽  
Single Case ◽  
Life Quality ◽  
Transforming Growth Factor Β1 ◽  
Intervertebral Discs ◽  
Review Literature ◽  
Background Information ◽  
Disc Disease ◽  
Case Study Report ◽  
Osteogenic Protein

The pathophysiology of the intervertebral discs plays a significant role in the people’s life quality. There is not adequate research done in the pathogenesis and treatment of intervertebral disc degeneration. Alternately, self-educated physiology offers a novel and noninvasive method to reverse the degenerated discs. In this single case study, report attempts have been made to highlight the effect of the self-educative physiology, on magnetic resonance imaging investigations, of progressive healing, on the degenerated intervertebral discs. Based on this novel method, an effort has been made to review literature on the degeneration of intervertebral discs and available mode of treatments and then to propose a hypothesis for the biochemical mechanisms of healing. The idea is that transforming growth factor-β1 from seminal plasma secretions may contribute to releasing the osteogenic protein- 1 which induces nucleus pulposus and annulus fibrosus cells in intervertebral discs for repairs. In addition, the patient’s medical history is presented with background information.

scholarly journals Degeneration of Lumbar Intervertebral Discs: Characterization of Anulus Fibrosus Tissue and Cells of Different Degeneration Grades

2020 ◽  
Vol 21 (6) ◽  
pp. 2165
Author(s):  
Stefan Stich ◽  
Michal Jagielski ◽  
Anja Fleischmann ◽  
Carola Meier ◽  
Patricia Bussmann ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transforming Growth Factor ◽  
Cultured Cells ◽  
Transforming Growth Factor Β1 ◽  
Intervertebral Discs ◽  
Bone Morphogenetic Protein 2 ◽  
Anulus Fibrosus ◽  
Morphogenetic Protein ◽  
Factor Α

Intervertebral disc (IVD) herniation and degeneration is a major source of back pain. In order to regenerate a herniated and degenerated disc, closure of the anulus fibrosus (AF) is of crucial importance. For molecular characterization of AF, genome-wide Affymetrix HG-U133plus2.0 microarrays of native AF and cultured cells were investigated. To evaluate if cells derived from degenerated AF are able to initiate gene expression of a regenerative pattern of extracellular matrix (ECM) molecules, cultivated cells were stimulated with bone morphogenetic protein 2 (BMP2), transforming growth factor β1 (TGFβ1) or tumor necrosis factor-α (TNFα) for 24 h. Comparative microarray analysis of native AF tissues showed 788 genes with a significantly different gene expression with 213 genes more highly expressed in mild and 575 genes in severe degenerated AF tissue. Mild degenerated native AF tissues showed a higher gene expression of common cartilage ECM genes, whereas severe degenerated AF tissues expressed genes known from degenerative processes, including matrix metalloproteinases (MMP) and bone associated genes. During monolayer cultivation, only 164 differentially expressed genes were found. The cells dedifferentiated and altered their gene expression profile. RTD-PCR analyses of BMP2- and TGFβ1-stimulated cells from mild and severe degenerated AF tissue after 24 h showed an increased expression of cartilage associated genes. TNFα stimulation increased MMP1, 3, and 13 expression. Cells derived from mild and severe degenerated tissues could be stimulated to a comparable extent. These results give hope that regeneration of mildly but also strongly degenerated disc tissue is possible.

AGE-DEPENDENT EXPRESSION OF TRANSFORMING GROWTH FACTOR β1 (TGF-β1) AND ITS RECEPTORS, AND AGE-RELATED STIMULATORY EFFECT OF TGF-β1 ON PROTEOGLYCAN SYNTHESIS IN RAT INTERVERTEBRAL DISCS

2000 ◽  
Vol 04 (03) ◽  
pp. 151-159 ◽  
Author(s):  
Shin'ya Okuda ◽  
Takanobu Nakase ◽  
Kazuo Yonenobu ◽  
Kenta Ariga ◽  
Wenxiang Meng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Intervertebral Discs ◽  
Proteoglycan Synthesis ◽  
Type I ◽  
Rt Pcr ◽  
Age Related ◽  
Tgf Β1

Age-related alterations of gene expression of transforming growth factor β1 (TGF-β1) and its receptors ( T β Rs ) in tissues derived from rat intervertebral discs were assessed together with TGF-β1-dependent proteoglycan synthesis by the cultured disc cells. Disc tissues and cells were individually harvested from two sites of the coccygeal vertebrae, namely the nucleus pulposus (NP) and annulus fibrosus (AF), which are major distinct components of the intervertebral discs. Semi-quantitative RT-PCR analysis indicated that the level of gene expression of TGF-β1/TGF-β1 receptor type I (TβR-I) of NP decreased with age. In AF, the level of TGF-β1/ T β Rs gene expression did not apparently differ with age. Consistent with the RT-PCR results, stimulation of proteoglycan synthesis by TGF-β1 in NP cells decreased with age. Proteoglycan synthesis by AF cells was also stimulated by TGF-β1. However, levels of this stimulation by AF cells were identical. The present findings indicate that the genetic expression of TGF-β1/ T β R -I and TGF-β1-dependent proteoglycan synthesis decreased with age in NP cells, and further suggest that a loss of proteoglycan synthesis with age in the intervertebral disc is at least in part due to the transcriptional down regulation of TGF-β1/ T βR-I and decreased synthetic ability of proteoglycans in response to TGF-β1 by NP cells.

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