Molecular Detection of Carbapenem Resistance in Acinetobacter Baumannii Isolated From Patients in Khorramabad City, Iran

2020 ◽  
Vol 20 (4) ◽  
pp. 543-549
Author(s):  
Zeinab Babaie ◽  
Somayeh Delfani ◽  
Faranak Rezaei ◽  
Fatemeh Norolahi ◽  
Somayeh Mahdian ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Acinetobacter Baumannii ◽  
Resistance Genes ◽  
High Resistance ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Biochemical Tests ◽  
Transfer Resistance ◽  
Drug Resistance Pattern ◽  
Wide Range

Background: Acinetobacter baumannii is an opportunistic pathogen, which causes a wide range of infections in hospitals, especially in intensive care units. Nowadays, due to the high resistance of Acinetobacter bumanni to antibiotics, this study, in addition to the phenotypic and genotypic investigations of drug resistance, focused on determining the molecular types of Acinetobacter baumannii isolated from patients in Khorramabad city by the pulsed-field gel electrophoresis (PFGE) method. Materials and Methods: In this cross-sectional study, 50 samples of Acinetobacter baumannii were collected from educational hospitals in Khorramabad city, Iran, from January to August 2015. They were identified in the laboratory using biochemical tests and culture methods. After determining the drug resistance pattern by the disc diffusion method and percentage of resistance genes to carbapenems, Acinetobacter baumannii isolates were analyzed using the PFGE method using the Apa1 enzyme. Results: The highest antibiotic resistance observed for Acinetobacter baumannii strains was against ampicillin-sulbactam (100%) and aztreonam (98%). The highest sensitivity was to polymixin B (100%) and colistin (94%), and also to the OXA-51-like gene present in all samples. The OXA-23-like gene was positive in 44 (88%) samples. PFGE results showed that Acinetobacterbaumannii strains had 33 different pulsotype patterns, of which 27 patterns had more than one strain and 23 had only one strain. Conclusion: Due to the high resistance of Acinetobacter baumannii and its ease of spread and its ability to transfer resistance genes, resistance control methods should be used in the disinfection of hospital areas. Hospital staff should observe hygiene standards and there should also be a reduction in antibiotic use.

scholarly journals Detection of Pan drug resistance OXA-48 producing Providencia in an ICU patient for the first time in Nepal

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Ranjit Sah ◽  
Shusila Khadka ◽  
Gentle Sunder Shrestha ◽  
Subhash Acharya ◽  
Diptesh Aryal ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Vital Signs ◽  
Carbapenem Resistance ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Common Mechanism ◽  
Disk Diffusion Method ◽  
Drug Resistance Pattern

Abstracts Background Resistance to antimicrobial agents of pathogenic bacteria has become a major problem in routine medical practices. Carbapenem resistance has long been increasing. The production of carbapenem- hydrolysing β-lactamases (carbapenamases), which include NDM, KPC, OXA-48, IMP-1 and VIM is the most common mechanism. Case presentation A 56 years old male presented with fever and mental changes with progressively decreasing sensorium for the last 3 days. He was admitted to Intensive care unit (ICU) with a diagnosis of meningoencephalitis. On day seven, he developed ventilator associated pneumonia due Klebsiella pnemoniae and Acinetobacter baumannii. He was on meropenem, but the isolates were susceptible to colistin, tigecyclin and amikacin solely. Hence, amikacin was started with addition of intravenous and nebulized colistin. Subsequently, vital signs improved with resolution of fever. However, on day 18, he developed fever once again with a drop in blood pressure. Inotropic support was maintained, and echinocandins and tigecycline were added to the regimen. Repeat blood and urine culture grew Providencia species, which were resistant to most of the drugs on phenotypic Kirby-Bauer disk diffusion method and are intrinsically resistant to colistin and tigecycline. Phenotypic detection of ESBL (combined disk method), MBL, KPCs, AmpC and co-producer were tested according to updated CLSI guideline and all were negative. But the Modified Hodges test was found to be positive. Consequenty, OXA-48 drug resistance pattern was brought into action by blank disc method according to A Tsakris et al., which revealed indentation of growth toward both EDTA and EDTA/PBA disk indicating production of OXA-48 carbapenamase. To confirm the resistance pattern we processed the isolated colonies for Xpert Carba-R (Cepheid) assay, which detected blaOXA-48 gene and confirmed the OXA-48 drug resistance pattern. Hence, the infecting organism was not susceptible to any of the antibiotics. The patient was kept under isolation and on 31th day of admission, he died of septic shock. Conclusions Carbapenamase production along with intrinsic colistin resistance in infecting bacterial pathogens can cause fatal outcomes in the resource limited countries like Nepal where new antibiotic combinations ceftazidime+ Avibactam, or aztreonam +avibactam are not available. Drug resistance patterns including OXA 48 producer should be characterized in all cases by standard phenotypic methods or by Xpert Carba-R assay and larger studies are required to know the exact burden of OXA 48 producer in Nepal.

scholarly journals Antibiotic Resistance, Plasmids and Integron Profile of Salmonella Species Isolated from Poultry Farm and Patients

2017 ◽  
Vol 15 (2) ◽  
pp. 209-214 ◽  
Author(s):  
Kohinur Begum ◽  
Sultana Juhara Mannan ◽  
Aliza Ahmed
Keyword(s):  
Drug Resistance ◽  
Nalidixic Acid ◽  
Plasmid Profile ◽  
Class I ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Poultry Farm ◽  
Serological Tests ◽  
Clinical Strains ◽  
Drug Resistance Pattern

A total of ten selected strains of Salmonella species, five from the environment of poultry farm and five from patients were included in this study. All strains were isolated and identified by using selective media, standard biochemical and serological tests. Antimicrobial susceptibility tests were performed by disc diffusion method using twelve commercial antibiotic discs of aztreonam, ceftriaxone, kanamycin, ciprofloxacin, gentamycin, chloramphenicol, ampicillin, erythromycin, cephalexin, tetracycline, cotrimoxazole and nalidixic acid. Plasmid profile and integron gene detection were conducted by Kado-Liu method and PCR, respectively. Only aztreonam, ceftriaxone, kanamycin and gentamycin were shown to be inhibitory to all strains. However, ciprofloxacin, chloramphenicol, amoxicilin, erythromycin, cephalexin, tetracycline, cotrimoxazole and nalidixic acid revealed different degrees of resistance pattern against environmental and clinical strains. Analysis of plasmid demonstrated that three environmental strains contained both plasmids of 140 Mda and 62 Mda. On the other hand, another three strains, one environmental and two clinical isolate only contained 140 Mda plasmid. All the plasmid containing strains (140 Mda and 62 Mda) exhibited same type of drug resistance pattern, whereas strains containing the 140 Mda plasmid only did not show similar type of resistance pattern. Therefore, no correlation was found between plasmid containing strains and drug resistance. Four environmental strains were also found to be positive for the class I integron and one clinical isolated was positive for class I integron gene conferring resistance to common antibiotics. However, none of the strains were found to contain class II integron. Therefore, the present study demonstrated that both environmental and clinical strains contain both large to middle size plasmids and integron I but not integron II. The plasmid and integron I containing strains experienced resistance to different antibiotics, used in the experiments.Dhaka Univ. J. Pharm. Sci. 15(2): 209-214, 2016 (December)

scholarly journals Antibiotic Resistance ofSalmonellaspp. Isolated from Shrimp Farming Freshwater Environment in Northeast Region of Brazil

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Fátima C. T. Carvalho ◽  
Oscarina V. Sousa ◽  
Edirsana M. R. Carvalho ◽  
Ernesto Hofer ◽  
Regine H. S. F. Vieira
Keyword(s):  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Shrimp Farming ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Plasmid Curing ◽  
Freshwater Environment ◽  
Disk Diffusion Method ◽  
Northeast Region ◽  
Drug Resistance Pattern

This study investigated the presence and antibiotic resistance ofSalmonellaspp. in a shrimp farming environment in Northeast Region of Brazil. Samples of water and sediments from two farms rearing freshwater-acclimatedLitopenaeus vannameiwere examined for the presence ofSalmonella. Afterwards,Salmonellaisolates were serotyped, the antimicrobial resistance was determined by a disk diffusion method, and the plasmid curing was performed for resistant isolates. A total of 30 (16.12%) of the 186 isolates were confirmed to beSalmonellaspp., belonging to five serovars:S. serovar Saintpaul,S. serovar Infantis,S. serovar Panama,S. serovar Madelia, andS. serovar Braenderup, along with 2 subspecies:S. entericaserovar houtenae andS. entericaserovar enterica. About twenty-three percent of the isolates were resistant to at least one antibiotic, and twenty percent were resistant to at least two antibiotics. Three strains isolated from water samples (pond and inlet canal) exhibited multiresistance to ampicillin, tetracycline, oxytetracycline, and nitrofurantoin. One of them had a plasmid with genes conferring resistance to nitrofurantoin and ampicillin. The incidence of bacteria pathogenic to humans in a shrimp farming environment, as well as their drug-resistance pattern revealed in this study, emphasizes the need for a more rigorous attention to this area.

scholarly journals Antibiotic resistance pattern of Salmonella spp. isolated from stool samples of hospitalized diarrheal patients in Bangladesh

2018 ◽  
Vol 3 (4) ◽  
pp. 534-538 ◽  
Author(s):  
Mohammad Sharif Uddin ◽  
Md Imranul Hoq ◽  
Mohammad Shaokat Ali ◽  
Md Mijanur Rahman ◽  
KM Shariful Islam
Keyword(s):  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Public Health Problem ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
Mortality And Morbidity ◽  
Antibiotic Susceptibility Test ◽  
Drug Resistance Pattern ◽  
Stool Samples

This study was carried out to investigate the drug resistance pattern of Salmonella spp. from diarrheal patients of under 5 years of age. Collected stool samples from the hospitalized diarrheal patients were analyzed for the presence of Salmonella spp. Among 350 stool samples from diarrheal patients, 15 (4%) were positive for the Salmonella spp. Antibiotic susceptibility test of the identified Salmonella spp. was performed according to Kirby-Bauer disc diffusion method. Eight commonly used antibiotics including azithromycin (15 μg), chloramphenicol (30 μg), Sulphamethaxole-trimethoprim (25 μg), metronidazole (50 μg), tetracycline (30 μg), doxycycline (30 μg), erythromycin (15 μg) and ciprofloxacin (5 μg) used to determine drug resistance pattern of the identified Salmonella spp. Majority of the isolates were multidrug resistant showed resistance against more than three drugs. 73% of Salmonella spp. was resistant to 2-4 drugs and 20% showed resistance to more than 5 drugs. We found that 100% Salmonella showed resistance to metronidazole and 87% were resistant to erythromycin. 7% isolates were resistant to chloramphenicol and doxycycline and 20% were resistant to ciprofloxacin. Antibiotic resistance is a serious public health problem worldwide and a leading cause of mortality and morbidity. This kind of situation, leads to great socioeconomic losses from the perspective of the patient, the hospital, and the whole society. To get rid form the drug resistance problem unnecessary, over and misuse of antibiotics should immediately prohibited and policy of judicious use of antibiotics should be strengthened.Asian J. Med. Biol. Res. December 2017, 3(4): 534-538

scholarly journals Biofilm Formation and blaOXA Genes Detection Among Acinetobacter baumannii from Clinical Isolates in a Tertiary Care Kirtipur Hospital, Nepal

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Upasana Ghimire ◽  
Rupesh Kandel ◽  
Mary Neupane ◽  
Sanjit Shrestha ◽  
Sudeep K C ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Biofilm Formation ◽  
Tertiary Care ◽  
Carbapenem Resistance ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
Clinical Settings ◽  
Drug Resistance Pattern ◽  
Acinetobacter Spp

(1) Background: Acinetobacter baumannii has emerged as a leading cause of nosocomial infections as they are capable of evolving resistance to various classes of antibiotics. The ability of A. baumannii to form biofilm might also be associated with increased antibiotic resistance and hence treatment failure. This study was carried to associate the biofilm formation with the drug resistance pattern of A. baumannii and to detect blaOXA-23, blaOXA-24, and blaOXA-51 from carbapenem resistance isolates. (2) Methods: Among different clinical samples, a total of 19 Acinetobacter spp. were identified with conventional microbiological procedures. The biofilm production was determined by a quantitative adherence assay. The antimicrobial susceptibility test was carried out by the Kirby-Bauer disc diffusion method, carbapenemase production detection was confirmed by Modified Hodge Test. And target resistant genes were detected by polymerase chain reaction. (3) Results: Out of 90 clinical specimens, 64.44% (58/90) showed bacterial growth. Whereas, 32.75% (19/58) isolates were identified as A. baumannii. Among all A. baumannii isolates, 84.21% (16/19) were multidrug-resistance and 63.16% (12/19) carbapenem resistance phenotypically. blaOXA-51 was detected in all the isolates and blaOXA-23 was detected only in 63.16% (12/19) isolates. However, blaOXA-24 was not detected in any of the isolates. Among A. baumannii, 89.47% (17/19) isolates produced biofilm with 47.37% (9/19) strong biofilm producers. (4) Conclusions: In the majority of MDR A. baumannii, blaOXA-51 and blaOXA-23 were detected as the determinant factor for carbapenem resistance having a direct relation with biofilm formation. This study provided a valuable clue for the management of A. baumannii infections in clinical settings.  

Changing patterns and widening of antibiotic resistance inShigellaspp. over a decade (2000–2011), Andaman Islands, India

2014 ◽  
Vol 143 (3) ◽  
pp. 470-477 ◽  
Author(s):  
D. BHATTACHARYA ◽  
H. BHATTACHARYA ◽  
D. S. SAYI ◽  
A. P. BHARADWAJ ◽  
M. SINGHANIA ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Wide Spectrum ◽  
Resistance Pattern ◽  
Third Generation ◽  
Isolation And Identification ◽  
Antimicrobial Drugs ◽  
Drug Resistance Pattern ◽  
Resistance Patterns ◽  
Third Generation Cephalosporins

SUMMARYThis study is a part of the surveillance study on childhood diarrhoea in the Andaman and Nicobar Islands; here we report the drug resistance pattern of recent isolates ofShigellaspp. (2006–2011) obtained as part of that study and compare it with that ofShigellaisolates obtained earlier during 2000–2005. During 2006–2011, stool samples from paediatric diarrhoea patients were collected and processed for isolation and identification ofShigellaspp. Susceptibility to 22 antimicrobial drugs was tested and minimum inhibitory concentrations were determined for third-generation cephalosporins, quinolones, amoxicillin-clavulanic acid combinations and gentamicin. A wide spectrum of antibiotic resistance was observed in theShigellastrains obtained during 2006–2011. The proportions of resistant strains showed an increase from 2000–2005 to 2006–2011 in 20/22 antibiotics tested. The number of drug resistance patterns increased from 13 in 2000–2005 to 43 in 2006–2011. Resistance to newer generation fluoroquinolones, third-generation cephalosporins and augmentin, which was not observed during 2000–2005, appeared during 2006–2011. The frequency of resistance inShigellaisolates has increased substantially between 2000–2006 and 2006–2011, with a wide spectrum of resistance. At present, the option for antimicrobial therapy in shigellosis in Andaman is limited to a small number of drugs.

scholarly journals Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Helal F. Hetta ◽  
Israa M. S. Al-Kadmy ◽  
Saba Saadoon Khazaal ◽  
Suhad Abbas ◽  
Ahmed Suhail ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Acinetobacter Baumannii ◽  
Biofilm Formation ◽  
Microtiter Plate ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Infection Model ◽  
The Impact

AbstractWe aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and characterized using TEM, XRD and FTIR spectroscopy. A. baumannii (n = 200) were isolated and identified. Resistance pattern was determined and virulence genes (afa/draBC, cnf1, cnf2, csgA, cvaC, fimH, fyuA, ibeA, iutA, kpsMT II, PAI, papC, PapG II, III, sfa/focDE and traT) were screened using PCR. Biofilm formation was evaluated using Microtiter plate method. Then, the antimicrobial activity of AgNPs was evaluated by the well-diffusion method, growth kinetics and MIC determination. Inhibition of biofilm formation and the ability to disperse biofilms in exposure to AgNPs were evaluated. The effect of AgNPs on the expression of virulence and biofilm-related genes (bap, OmpA, abaI, csuA/B, A1S_2091, A1S_1510, A1S_0690, A1S_0114) were estimated using QRT-PCR. In vitro infection model for analyzing the antibacterial activity of AgNPs was done using a co-culture infection model of A. baumannii with human fibroblast skin cell line HFF-1 or Vero cell lines. A. baumannii had high level of resistance to antibiotics. Most of the isolates harbored the fimH, afa/draBC, cnf1, csgA and cnf2, and the majority of A. baumannii produced strong biofilms. AgNPs inhibited the growth of A. baumannii efficiently with MIC ranging from 4 to 25 µg/ml. A. baumannii showed a reduced growth rate in the presence of AgNPs. The inhibitory activity and the anti-biofilm activity of AgNPs were more pronounced against the weak biofilm producers. Moreover, AgNPs decreased the expression of kpsMII , afa/draBC,bap, OmpA, and csuA/B genes. The in vitro infection model revealed a significant antibacterial activity of AgNPs against extracellular and intracellular A. baumannii. AgNPs highly interrupted bacterial multiplication and biofilm formation. AgNPs downregulated the transcription level of important virulence and biofilm-related genes. Our findings provide an additional step towards understanding the mechanisms by which sliver nanoparticles interfere with the microbial spread and persistence.

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