Detection of TGF-β1, HGF, IGF-1 and IGF-1R in Cleft Affected Mucosa of the Lip

Background: Orofacial clefts are one of the most common birth defects with multifactorial and only partly understood morphopathogenesis. Objective: The aim of this study was to evaluate the presence of TGF-β1, HGF, IGF-1 and IGF-1R in cleft affected mucosa of the lip. Methods: Lip mucosa tissue samples were obtained during surgical cleft correction from seven 2 to 6 months old children. Prepared tissue sections were stained by immunohistochemistry for TGF-β1, HGF, IGF-1 and IGF-1R. The intensity of staining was graded semiquantitatively. Results: We found numerous TGF-β1 and HGF-containing epithelial and connective tissue cells, moderate number of IGF-1 immunoreactive cells and even less pronounced presence of IGF-1R-positive cells. Conclusion: TGF-β1 and HGF are present in defective epithelia and soft tissue in cleft affected lip. Expressions of IGF-1 and IGF-1R show significant differences, and both factors play a role in the morphopathogenesis of clefts.

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Phenotypical Differences in Connective Tissue Cells Emerging from Microvascular Pericytes in Response to Overexpression of PDGF-B and TGF-β1 in Normal Skin in Vivo

American Journal Of Pathology ◽

10.1016/j.ajpath.2013.01.054 ◽

2013 ◽

Vol 182 (6) ◽

pp. 2132-2146 ◽

Cited By ~ 11

Author(s):

Alejandro Rodriguez ◽

Tomas Friman ◽

Marcin Kowanetz ◽

Tijs van Wieringen ◽

Renata Gustafsson ◽

...

Keyword(s):

Connective Tissue ◽

Normal Skin ◽

Tissue Cells ◽

Tgf Β1

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TGF β1 and PDGF AA override Collagen type I inhibition of proliferation in human liver connective tissue cells

BMC Gastroenterology ◽

10.1186/1471-230x-4-30 ◽

2004 ◽

Vol 4 (1) ◽

Cited By ~ 6

Author(s):

Alvaro T Geremias ◽

Marcelo A Carvalho ◽

Radovan Borojevic ◽

Alvaro NA Monteiro

Keyword(s):

Connective Tissue ◽

Human Liver ◽

Collagen Type ◽

Collagen Type I ◽

Type I ◽

Inhibition Of Proliferation ◽

Tissue Cells ◽

Tgf Β1 ◽

Liver Connective Tissue Cells

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Evaluation of TGF-β1 and EGFR in Cleft Affected Lip Mucosa

Acta medica Lituanica ◽

10.15388/amed.2021.28.1.14 ◽

2021 ◽

Vol 28 (1) ◽

pp. 14

Author(s):

Olga Rimdenoka ◽

Māra Pilmane

Keyword(s):

Epithelial Cells ◽

Lamina Propria ◽

Oral Mucosa ◽

Rank Correlation ◽

Control Group ◽

Orofacial Cleft ◽

Tissue Samples ◽

Moderate Number ◽

Healthy Mucosa ◽

Tgf Β1

Background. The morphopathogenesis of orofacial cleft development is only partly understood; therefore, it is important to identify factors, which possibly could be involved in it. The aim of the study was to evaluate the distribution of TGF-β1 and EGFR-containing cells in cleft affected lip mucosa.Materials and Methods. The study group included lip mucosa tissue samples from 14 patients with orofacial cleft. The control group contained 11 healthy oral mucosa tissue samples. The tissue sections were stained by immunohistochemistry for TGF-β1 and EGFR. The expression of positive structures was graded semiquantitatively. IBM SPSS 26.0 was used for statistical analysis, Spearman`s rank correlation and Mann-Whitney U tests were performed.Results. Mostly few to moderate number (+/++) of TGF-β1-containing cells was found in epithelium, also the same number of fibroblasts and macrophages was seen in the lamina propria of cleft affected lip mucosa. Meanwhile, healthy oral mucosa on average demonstrated a moderate number (++) of TGF-β1-containing epithelial cells, fibroblasts, and macrophages. A variable, mostly indistinct number of EGFR-containing cells was seen in the epithelium of cleft affected lip mucosa, meanwhile, mostly no (0) EGFR positive cells were found in the epithelium of healthy mucosa. Statistically significantly less TGF-β1-containing cells were found in the epithelium of cleft affected lip mucosa than in the healthy mucosa (U=33.000; p=0.015). Also, the lamina propria of cleft affected lip mucosa showed statistically significantly less TGF-β1 immunoreactive fibroblasts and macrophages than the healthy mucosa (U=28.500; p=0.006).Conclusions. The decreased number of TGF-β1-containing epithelial cells, fibroblasts and macrophages in cleft affected lip mucosa proves the role of problematic tissue remodelation in the cleft pathogenesis. The distribution of EGFR in cleft affected and healthy mucosa is similar and possibly does not play a role in the cleft development of humans.

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Discovery of intracellular 2,8 dihydroxyadenine crystals in bovine lymphatic and hepatic cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010012881x ◽

1987 ◽

Vol 45 ◽

pp. 906-907

Author(s):

W. E. Rigsby ◽

D. M. Hinton ◽

V. J. Hurst ◽

P. C. McCaskey

Keyword(s):

Polarized Light ◽

Paraffin Sections ◽

Tissue Samples ◽

Whole Cells ◽

Tissue Sections ◽

Hepatic Cells ◽

Slaughter House ◽

Mammalian Tissues ◽

Carbon Coated ◽

Cellular Components

Crystalline intracellular inclusions are rarely seen in mammalian tissues and are often difficult to positively identify. Lymph node and liver tissue samples were obtained from two cows which had been rejected at the slaughter house due to the abnormal appearance of these organs in the animals. The samples were fixed in formaldehyde and some of the fixed material was embedded in paraffin. Examination of the paraffin sections with polarized light microscopy revealed the presence of numerous crystals in both hepatic and lymph tissue sections. Tissue sections were then deparaffinized in xylene, mounted, carbon coated, and examined in a Phillips 505T SEM equipped with a Tracor Northern X-ray Energy Dispersive Spectroscopy (EDS) system. Crystals were obscured by cellular components and membranes so that EDS spectra were only obtainable from whole cells. Tissue samples which had been fixed but not paraffin-embedded were dehydrated, embedded in Spurrs plastic, and sectioned.

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Increased matrix gene expression by glucose in rat neural connective tissue cells in culture

Diabetes ◽

10.2337/diabetes.40.5.605 ◽

1991 ◽

Vol 40 (5) ◽

pp. 605-611 ◽

Cited By ~ 7

Author(s):

P. Muona ◽

J. Peltonen ◽

S. Jaakkola ◽

J. Uitto

Keyword(s):

Gene Expression ◽

Connective Tissue ◽

Cells In Culture ◽

Matrix Gene ◽

Tissue Cells

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Palatal soft-tissue changes after connective tissue harvesting – preliminary results of a 3D volumetric analysis

10.26226/morressier.5ac383292afeeb00097a472f ◽

2018 ◽

Author(s):

Nuno Santos

Keyword(s):

Soft Tissue ◽

Connective Tissue ◽

Volumetric Analysis ◽

Preliminary Results ◽

Tissue Changes ◽

Soft Tissue Changes

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Rapid brain structure and tumour margin detection on whole frozen tissue sections by fast multiphotometric mid-infrared scanning

Scientific Reports ◽

10.1038/s41598-021-90777-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Tim Kümmel ◽

Björn van Marwick ◽

Miriam Rittel ◽

Carina Ramallo Guevara ◽

Felix Wühler ◽

...

Keyword(s):

Imaging System ◽

Frozen Section ◽

Computational Effort ◽

Primary Hepatocellular Carcinoma ◽

Measuring System ◽

Sufficient Information ◽

Tissue Samples ◽

Mid Infrared ◽

Tissue Sections ◽

Frozen Tissue

AbstractFrozen section analysis is a frequently used method for examination of tissue samples, especially for tumour detection. In the majority of cases, the aim is to identify characteristic tissue morphologies or tumour margins. Depending on the type of tissue, a high number of misdiagnoses are associated with this process. In this work, a fast spectroscopic measurement device and workflow was developed that significantly improves the speed of whole frozen tissue section analyses and provides sufficient information to visualize tissue structures and tumour margins, dependent on their lipid and protein molecular vibrations. That optical and non-destructive method is based on selected wavenumbers in the mid-infrared (MIR) range. We present a measuring system that substantially outperforms a commercially available Fourier Transform Infrared (FT-IR) Imaging system, since it enables acquisition of reduced spectral information at a scan field of 1 cm2 in 3 s, with a spatial resolution of 20 µm. This allows fast visualization of segmented structure areas with little computational effort. For the first time, this multiphotometric MIR system is applied to biomedical tissue sections. We are referencing our novel MIR scanner on cryopreserved murine sagittal and coronal brain sections, especially focusing on the hippocampus, and show its usability for rapid identification of primary hepatocellular carcinoma (HCC) in mouse liver.

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