Phytochemical Profiles, Antioxidant and Antibacterial Activities of 11 Phellinus Mushrooms Collected in Thailand

2019 ◽  
Vol 9 (2) ◽  
pp. 144-156 ◽  
Author(s):  
Punjaporn Sunthudlakhar ◽  
Pongtip Sithisarn ◽  
Bhusita Wannissorn ◽  
Siripen Jarikasem ◽  
Piyanuch Rojsanga
Keyword(s):  
Protocatechuic Acid ◽  
Antioxidant Activities ◽  
Antibacterial Activities ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Total Carbohydrate ◽  
Frap Assay ◽  
Phytochemical Profiles ◽  
Mushroom Extracts

Background:Phellinus mushrooms are locally available in Thailand. They have been traditionally used for medicinal purposes including tonics, for cancer treatment and for immune system stimulation. </P><P> Objective: To evaluate the in vitro antioxidant, antibacterial activities and the phytochemical information of the extracts from 11 selected Phellinus mushrooms collected in Thailand.Methods:Eleven Phellinus mushrooms were collected from the Northeastern part of Thailand. Their ethanol and water extracts were tested for in vitro antioxidant activities using a DPPH scavenging assay, FRAP assay and TBARS method while the antibacterial effects were determined by agar disk diffusion and broth microdilution methods. The phytochemical profiles of the Phellinus mushroom extracts were evaluated using TLC and HPLC techniques. The total phenolic and total flavonoid contents of the extracts were evaluated by Folin-Ciocalteu and aluminium chloride methods, respectively. While total carbohydrate and total triterpene contents were evaluated by phenolsulfuric acid and vanillin perchloric acid methods, respectively.Results:Most ethanol extracts from Phellinus mushrooms exhibited moderate antioxidant effects especially extracts from P. everhartii, P. hippophaëicola and P. pini var. microporus while P. igniarius var. cinereus ethanol extract exhibited inhibitory effects to Helicobacter pylori both normal and resistant strains, this extract also showed the strongest inhibitory effect against Staphylococcus aureus. Most Phellinus mushroom extracts exhibited similar TLC and HPLC fingerprints with the chromatographic bands corresponding to phenolics, flavonoids and terpenoids. Protocatechuic acid was identified in most Phellinus mushroom extracts.Conclusion:Phellinus mushrooms especially, P. everhartii could be developed as sources of antioxidants while P. igniarius var. cinereus indicated the potential to inhibit H. pylori. Protocatechuic acid could be used as a marker for quality control of Phellinus mushroom raw materials and extracts.

scholarly journals ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF SELECTED PLANTS USED BY POPULATIONS FROM JURUENA VALLEY, LEGAL AMAZON, BRAZIL

2017 ◽  
Vol 9 (5) ◽  
pp. 179
Author(s):  
Larissa Irene Da Silva ◽  
Arunachalam Karuppusamy ◽  
Fabio Miyajima ◽  
Ivana Maria Povoa Violante ◽  
Isanete Geraldini Costa Bieski ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Shigella Flexneri ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Moderate Activity ◽  
Cedrela Odorata ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Mato Grosso

Objective: The purpose of this study was to evaluate selected Brazilian plants from Juruena valley region of Mato Grosso, for their in vitro antimicrobial and antioxidant activities.Methods: The powder obtained from different parts of the twenty-six (26) plants were macerated in hydroethanolic solution to obtain the extracts. The hydroethanolic extracts were tested for their in vitro antimicrobial activity by determining the MIC using broth microdilution. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, ferric reducing antioxidant power (FRAP) and nitric oxide (NO) methods were used for the determination of antioxidant activities. Correlation between classes of secondary metabolites and antioxidant activity was assessed.Results: Phanera glabra extract (HEPg) showed broad antibacterial spectrum, presenting the best activity against Klebsiella pneumoniae. Hevea microphylla extract (HEHm) presented a narrow spectrum of antibacterial activity with strong effect against Shigella flexneri. The only plant with broad spectrum antifungal activity was Bertholletia excelsa (HEBe), with moderate activity against strains of Aspergillus and Candida. The following extracts were prominent regarding their activities in the DPPH and FRAP assays-HEBe, Cariniana rubra (HECr) and in the FRAP assay alone, Cedrela odorata (HECo) and HEPg. None of the extracts was active in the NO assay. A significant association was observed between DPPH activity and the total phenolic contents.Conclusion: Our results justified the use of some of the investigated plants in the Brazilian ethnomedicine. The antibacterial activities of these plants are bacteriostatic in nature. These findings support that a number of investigated plants could be a valuable source of new antioxidant and antimicrobial compounds that can potentially deliver novel mechanisms of actions.However, further studies are required.

Phytochemical composition and antioxidant activity of Malus baccata (L.) Borkh. fruits

2020 ◽  
Vol 3 (1) ◽  
pp. 47
Author(s):  
Nadezhda Petkova ◽  
Manol Ognyanov ◽  
Blaga Inyutin ◽  
Petar Zhelev ◽  
Panteley Denev
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Titratable Acidity ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Frap Assay ◽  
Phytochemical Composition ◽  
Malus Baccata

Crab apple (Malus baccata (L.) Borkh.) was mainly distributed in Europe as an ornamental plant, but the nutritional properties of its edible fruits were not fully revealed. The aim of the current study was to characterize the phytochemical composition of ripen carb apple fruits and to evaluate their nutritional and antioxidant potentials. The fruits were assayed for moisture and ash content, proteins, lipids, carbohydrates, titratable acidity (TA), pH, total phenolic compounds and natural pigments. Among the analyzed carbohydrates cellulose was found in the highest content (6% dw), followed by sugars (sucrose, glucose and fructose) and 1.8 % dw uronic acids. The total chlorophylls and carotenoids contents in their fruits were 6.51 and 4.80 μg/g fw, respectively. Total monomeric anthocyanins were not detected. The highest content of total phenolic compounds (2.67 mg GAE/g fw) was found in 95 % ethanol extract from fruits, while the total flavonoids were relatively low – 0.1 mg QE/g fw. DPPH assay (17.27 mM TE/g fw) and FRAP assay (14.34 mM TE/g fw) demonstrated in vitro antioxidant activities of crabapple. Malus baccata fruits were evaluated as a rich source of dietary fibers and phenolic compounds with significant antioxidant potential that could be used in human nutrition.

Functionalized silver nanoparticles synthesized from marine algae: In vitro Evaluation of Antibacterial Potential

2021 ◽  
Vol 16 (8) ◽  
pp. 38-49
Author(s):  
Siva Kumar Kandula ◽  
Satyanarayana Swamy Cheekatla ◽  
Venkata Satya Mahesh Kumar Metta ◽  
Venkata Rajagopal Saladi
Keyword(s):  
Silver Nanoparticles ◽  
Antioxidant Activities ◽  
Algal Species ◽  
Synthesis Method ◽  
Radical Scavenging ◽  
Visible Spectrum ◽  
Total Phenolic ◽  
Face Centered Cubic ◽  
Frap Assay

Natural antioxidants, in particular phenolic derivatives, are used efficiently to combat against oxidative induced tissue damages. The objective of the study is to determine the antioxidant potential of methanolic extracts obtained from eight marine algal species (Enteromorpha compressa, Chaetomorpha antennina, Caulerpa racemosa, Caulerpa taxifolia, Sargassum vulgare, Padina tetrastromatica, Amphiroa fragilissima and Gracilaria corticata) by assessing their total phenolic content, DPPH scavenging assay, FRAP assay, H2O2 radical and superoxide radical scavenging activities. Among them, P.tetrastromatica, S.vulgare, E.compressa, C.taxifolia display significant antioxidant activities. Further, the aqueous extracts of these four algae are used for bioreduction of silver nitrate to silver nanoparticles (AgNPs) by green synthesis method at room temperature. UV-Visible spectrum revealed the surface plasmon resonance at 430 and 440nm. The characterizations of AgNPs by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) studies revealed the bioreduction and capping of AgNPs. XRD analysis elucidated the synthesized nanoparticles having face centered cubic crystalline geometry, with a mean size of 17 nm. The nanoparticles have better antimicrobial activity against cocci shaped than the rod shaped bacteria. The minimum inhibitory concentration and minimum bactericidal concentration exhibit more activity against S.aureus and B.cereus rather than E.coli.

scholarly journals Antioxidant capacity of Melissa Officinalis L. on Biological Systems

2018 ◽  
Vol 43 (3) ◽  
pp. 19
Author(s):  
Juliana Metzner Franco ◽  
Silvana Marina Piccoli Pugine ◽  
Antônio Márcio Scatoline ◽  
Mariza Pires De Melo
Keyword(s):  
Ascorbic Acid ◽  
Oxidative Damage ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Protective Action ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Melissa Officinalis ◽  
Frap Assay

The aim of the present study was to evaluate in vitro antioxidant capacity of Melissa extract (ME) (Melissa officinalis L.) and its protective effect on peroxyl radical-induced oxidative damage in erythrocytes. ME used in present study was obtained by rota-evaporation of the crude extract (ethanol:water/dried leaves). Total phenolic and flavonoids contend determination, 176.8 ± 13.2 mg GAE/g dw and  26.2 ± 3.2 mg QE/g dw, respectively).  Total equivalent antioxidant activities, TEAC in mg TE/g dw, were 61.4 ± 5.5 and 512.4 ± 77.2 for respective FRAP assay and DPPH• radical-scavenging. The ME acts as an antioxidant on NO and O2•-, when ME exerted a higher antioxidant action on NO scavenging to compared to the ascorbic acid (1.9 times), however, the antioxidant capacity of ME on O2•- was lower than ascorbic acid (5.6 times). The values of hemolysis inhibition from ME (IC50, 2.0 ± 0.5 mg/mL) were higher than ascorbic acid (IC50, 7.1 ± 1.8 mg/mL). Extract of Melissa was able to eliminate biological free radicals, suggesting a potential to prevent oxidative damage in vivo. In fact, the ME exerted protective action on cell membrane lysis in situ.

scholarly journals Bioactivities of Water Extract and Essential Oil from the Mace of Myristica fragrans Houtt

2018 ◽  
Vol 26 (2) ◽  
pp. 099
Author(s):  
Adit Widodo Santoso ◽  
Adelina Simamora ◽  
Adelina Simamora ◽  
Kris Herawan Timotius ◽  
Kris Herawan Timotius
Keyword(s):  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Traditional Use ◽  
Myristica Fragrans

<p><em>Myristica fragrans</em> Houtt (nutmeg) is used as a spice and flavour for food and beverages. It has been traditionally used to treat a number of medical conditions, including diabetes mellitus. The study was undertaken to scientifically validate the traditional use of mace from <em>M.</em><em> </em><em>Fragrans</em><strong>. </strong>The objectives of this study were to evaluate α-glucosidase inhibition, antioxidant and antibacterial activities of water extract (WE) and essential oil (EO) from <em>M.</em><em> </em><em>fragrans</em> mace.  Both WE and EO were evaluated for their α-glucosidase inhibitory activities <em>in vitro</em> and their antioxidant activities based on DPPH radical scavenging assay. Standard compounds were used for every test. Total phenolic and flavonoid contents of both extracts were also determined. The extracts were also tested for their antibacterial activities against six different bacteria by a well diffusion method. Both extracts showed inhibition activities against α-glucosidase, with WE showed stronger activity than EO (IC<sub>50</sub> = 1.86 and 8.15 mg/ml). Good radical scavenging activities were observed for both extracts, with WE showed stronger activity than EO (IC<sub>50</sub> = 1.51 and 4.59 mg/ml). WE showed higher content in phenolic than EO (47.84 and 37.21 mg GAE/100 g DW). Flavonoid content in WE was also higher than EO (215.36 and 30.12 mg RE/ml). Based on the well diffusion method, only EO exhibited antibacterial activities, with inhibition zone in the range 1.03 – 1.30 mm.  The strongest activity was observed against<em> Staphylococcus mutans</em>. The results indicate WE and EO can be exploited further for pharmacological uses, in particular for their antidiabetic and antioxidant activities. </p>

Estimation of Total Phenolics and Flavonoid Content and In-vitro Antioxidant Activity of Drypetes sepiaria Stem (Wight and Arn.) Pax and K. Hoffm.

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Packia M. ◽  
Daffodil D. ◽  
Tresina S. ◽  
Mohan R.
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Model Systems ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Ethanol Extracts ◽  
In Vitro Antioxidant Activity

The total phenolics, flavonoids and in vitro antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts of stem of Drypetes sepiaria were determined using various antioxidant model systems viz, DPPH, hydroxyl, superoxide, ABTS and reducing power. Total phenolic content was estimated by Folin-Ciocalteau method. Flavonoids were determined by Aluminium chloride method. The total phenolics and flavonoids contents were found to be 0.81 g 100 g-1 and 1.12g 100 g-1 respectively in the methanol extract. Among the solvent tested, methanol and ethanol extracts of D. sepiaria stem showed potent in vitro antioxidant activities. This investigation explored that D. sepiaria stem is a potential source of natural antioxidant.

scholarly journals Antioxidant activity and total phenolic and flavonoid contents of Chrysopogon aucheri (Boiss.) Stapf

2017 ◽  
Vol 63 (2) ◽  
pp. 42-47
Author(s):  
Maryam Sadoughinia ◽  
Jinous Asgarpanah
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Aluminium Chloride ◽  
Biological Research ◽  
Total Phenolic ◽  
Total Extract ◽  
Phenolic And Flavonoid

Summary Introduction: Chrysopogon aucheri is a species native to the South of Iran. It is used for the treatment of some oxidative-based disorders. Objective: Due to lack of biological research on C. aucheri, we were prompted to investigate the antioxidant activities of leaf total extract and different sub-fractions for the first time. The phenolic and flavonoid contents were also determined in the leaves as the interaction between these components and the antioxidant activity. Methods: The antioxidant effects of total extract and sub-fractions were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. Total phenolic and flavonoid contents were also determined by Folin-Ciocalteu and aluminium chloride colorimetric methods, respectively. Results: Total extract of C. aucheri leaves was found to possess desirable antioxidant activity in vitro in comparison with standard antioxidant BHT (butylated hydroxytoluene). The highest phenolic and flavonoid contents were observed in the methanol sub-fraction. Results showed weak and moderate correlations with phenolics and flavonoids, respectively, and therefore other metabolites of C. aucheri leaves might be responsible for antioxidant activities. Conclusion: The results suggested that C. aucheri leaves possess desirable antioxidant activity when compared with BHT and support the ethnomedicinal claims of the use of the leaves in the management of some oxidative-based diseases.

scholarly journals In Vitro Antioxidant Activities of Three Selected Dates from Tunisia (Phoenix dactyliferaL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Wissal Kchaou ◽  
Fatma Abbès ◽  
Hamadi Attia ◽  
Souhail Besbes
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Food Additives ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Scavenging Activity ◽  
Frap Assay

Second-grade dates (Phoenix dactyliferaL.), with hard texture, from three selected Tunisian cultivars (Allig, Deglet Nour, and Bejo) were analysed from their antioxidant activities using DPPH radical scavenging activity, FRAP assay, H2O2scavenging activity, and metal chelating activity. Date extracts showed strong and concentration-dependant activity in all tested methods. The results showed that the best antioxidant activity was obtained in Allig, followed by Bejo and Deglet Nour. Total phenolics, total flavonoids, carotenoids, and tannins were determined spectrophotometrically in three date extracts. Results indicated that date contained significantly different amounts of these compounds. In fact, Allig presented the highest antioxidant compounds, followed by Bejo and Deglet Nour. Correlation analyses indicated a positive linear relationship between antioxidant activities and total phenolic and flavonoid content of date. This study demonstrates the potential antioxidant activity with Tunisian date, where we can use these natural extracts as food additives in replacement of synthetic compounds.

scholarly journals Influence of the Storage in Bottle on the Antioxidant Activities and Related Chemical Characteristics of Wine Spirits Aged with Chestnut Staves and Micro-Oxygenation

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 106
Author(s):  
Sheila Oliveira-Alves ◽  
Sílvia Lourenço ◽  
Ofélia Anjos ◽  
Tiago A. Fernandes ◽  
Ilda Caldeira ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Chemical Characteristics ◽  
Low Molecular Weight ◽  
Nitrogen Control ◽  
Frap Assay ◽  
Phenolic Index ◽  
In Vitro Antioxidant Activity

Different ageing technology of wine spirits (WSs) has been investigated, but little has been published on the chemical evolution of aged WS during storage in bottle. The purpose of this study was to examine how 12 months of storage in bottle affected the evolution of antioxidant activity (DPPH, FRAP and ABTS assays), total phenolic index (TPI) and low molecular weight (LMW) compounds content of the WSs aged through alternative technology using three micro-oxygenation levels (MOX) and nitrogen control (N). Results revealed the ability of phenolic compounds from aged WSs to scavenge free radicals during storage in bottle. Among the in vitro antioxidant-activity methods, FRAP assay was the more effective to differentiate WSs according to the ageing technology. Concerning the overall influence of storage in bottle on antioxidant activity, and TPI and LMW compounds content, the higher results were obtained for the MOX modalities (O15, O30 and O60), which showed a similar evolution. In summary, this study provides innovative information, demonstrating that the differences between the aged WSs imparted throughout the ageing process (resulting from different MOX levels) were mostly retained, and only slight modifications during storage in bottle were found.

scholarly journals Quantification of total bioactive contents and evaluation of the antioxidant and antibacterial activities of crude extracts from Ephedra altissima Desf.

2021 ◽  
Vol 43 ◽  
pp. e52123
Author(s):  
Waffa Bouafia ◽  
Soumia Mouffouk ◽  
Hamada Haba
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Etoac Extract ◽  
Crude Extracts ◽  
Good Ability

The present work describes the in vitro biological activities of the crude extracts (petroleum ether, ethyl acetate and n-butanol) prepared from the species Ephedra altissima Desf. The estimation of total phenolic, flavonoid and tannin contents were carried out using the Folin-Ciocalteu, trichloroaluminum and acidified vanillin methods, respectively. The evaluation of the in vitro antioxidant activities were performed by three different methods namely: scavenging of the free radical ABTS, permanganate reducing antioxidant capacity, and potentiometric assay. In addition, the antibacterial activity was assessed by the agar disk diffusion assay against seven bacterial strains. The results of the phytochemical screening revealed the presence of several types of secondary metabolites. The EtOAc extract exhibited the highest content of phenols (125.62 ± 1.51 μg EGA mg-1 of extract). The greatest flavonoid and tannin contents were observed for n-BuOH extract (19.18 ± 0.39 µg EQ mg-1 of extract and 8.95 ± 1.70 μg EC mg-1 of extract, respectively). Moreover, the EtOAc extract revealed potent antioxidant activity in all the tested methods. Furthermore, the aqueous extract from the species E. altissima showed a good ability to reduce iron III to iron II with a value of 0.68 ± 0.3 moL eq L-1 in potentiometric assay. All the crude extracts (PE, EtOAc and n-BuOH) displayed inhibition of bacterial growth against at least three strains with values of MIC ranging from 3.125 to 50 µg mL-1. Therefore, these results suggest that Ephedra altissima could be used as an important source of natural bioactive compounds with antioxidant and antibacterial properties.

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