Biomimetic Synthesis of Silver Nanoparticles Using Bhimkol (Musa balbisiana) Peel Extract as Biological Waste: Its Antibacterial Activity and Role of Ripen Stage of the Peel

2020 ◽  
Vol 5 (1) ◽  
pp. 47-65
Author(s):  
Subhendu S. Bag ◽  
Anupama Bora ◽  
Animes K. Golder
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Agricultural Waste ◽  
Cost Effective ◽  
Biomimetic Synthesis ◽  
Biological Macromolecules ◽  
Spectroscopic Techniques ◽  
Musa Balbisiana ◽  
Resultant Solution ◽  
Average Size

Background: Utilization of plant extracts and agricultural waste has a great impact for the synthesis of AgNPs. Banana peels are such important agro waste which attracted us to use for the synthesis of silver nanoparticles. The biochemicals present in it have attracted us to use such banana peels. Methods: Thus, we report herein a cost-effective and environment-friendly synthesis of silver nanoparticles using Bhimkal (Musa balbisiana) peel aqueous extract as biological waste. About 5 g of freshly dried peels taken in 100 mL of water were shaken and heated at 80°C for 1 hour. The filtrate from the resultant solution was stored at 4°C and used as reducing as well as stabilizing agent for the preparation of AgNPs from AgNO3. We monitored the formation of silver nanoparticles by various spectroscopic techniques. Results: All the particles are almost spherical in morphology and the diameter of the mostly monodispersed AgNPs is in the range of 30-70 nm with an average size of 44.24 nm. Among the three stages of development (unripe, ripe, and blacken), we have found the ripening stage as most efficient in the highest yielding of AgNPs because of maximum presence of phenol containing biological macromolecules. The synthesized AgNPs showed moderate antibacterial activity against both gram negative bacteria as well as gram positive bacteria. Conclusion: The advantage of our biomimetic route to silver nanoparticles lies in the fact that we utilize peels as biological waste material both for the generation and stabilization of silver nanoparticles.

scholarly journals Biomimetic Synthesis of Silver Nanoparticles from Aqueous Extract of Saraca indica and its Profound Antibacterial Activity

2020 ◽  
Vol 11 (1) ◽  
pp. 8110-8120
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Silver Nanoparticle ◽  
Pathogenic Bacteria ◽  
Drug Formulation ◽  
Biomimetic Synthesis ◽  
Visible Absorption ◽  
The Stability ◽  
Average Size

The present findings were focused on green synthesis of silver nanoparticles through an aqueous extract of Saraca indica. The stability of the nanoparticle was achieved through the optimization of physico-chemical parameters. The sharp UV-visible absorption maximum at 400 was observed for biological synthesized silver nanoparticles. The spectroscopic analysis was thus used to assess the formation of silver nanoparticles. The AFM analysis did analyze the morphology of the nanocomposite, which was further confirmed through TEM micrograph. The electron micrograph image discloses that silver nanoparticles were polydispersed and dominantly as spherical with size ranges from 40nm to 100nm. The average size distribution was 49nm. The chemical reductions of Ag+ ions were further confirmed through FTIR. The biogenic silver nanoparticle and their drug formulation showed profound antibacterial activity against pathogenic bacteria. The flavonoids rich binding of silver nanoparticle showed great medicinal potential and can be used for the treatment of several harmful infectious diseases. Hence, plant-based metal nanoparticles meet the demand for less toxic formulation during drug development and its delivery.

scholarly journals Biological Synthesis of Silver Nanoparticles by Cell-Free Extract ofSpirulina platensis

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Gaurav Sharma ◽  
Nakuleshwar Dut Jasuja ◽  
Manoj Kumar ◽  
Mohammad Irfan Ali
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Large Scale ◽  
Cost Effective ◽  
Biological Synthesis ◽  
Cell Free Extract ◽  
Tem Analysis ◽  
High Antibacterial Activity ◽  
Vis Spectroscopy ◽  
Average Size

The present study explores biological synthesis of silver nanoparticles (AgNPs) using the cell-free extract ofSpirulina platensis. Biosynthesised AgNPs were characterised by UV-Vis spectroscopy, SEM, TEM, and FTIR analysis and finally evaluated for antibacterial activity. Extracellular synthesis using aqueous extract ofS. platensisshowed the formation of well scattered, highly stable, spherical AgNPs with an average size of 30–50 nm. The size and morphology of the nanoparticles were confirmed by SEM and TEM analysis. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilisation of AgNPs. Furthermore, the synthesised nanoparticles exhibited high antibacterial activity against pathogenic Gram-negative, that is,Escherichia coli, MTCC-9721;Proteus vulgaris, MTCC-7299;Klebsiella pneumoniae, MTCC-9751, and Gram-positive, that is,Staphylococcus aureus, MTCC-9542;S. epidermidis, MTCC-2639;Bacillus cereus, MTCC-9017, bacteria. The AgNPs had shown maximum zone of inhibition (ZOI) that is31.3±1.11inP. vulgaris. Use of such a microalgal system provides a simple, cost-effective alternative template for the biosynthesis of nanomaterials of silver in a large scale that could be of great use in biomedical applications.

scholarly journals An Investigation on the Antiproliferative and Antibacterial Activity of Silver Nanoparticles of Quercus infectoria and Daucus carota subsp sativum

2021 ◽  
pp. 33-46
Author(s):  
S. Prathimaa ◽  
J. Anbumalarmathi ◽  
S. Aruna Sharmili
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Daucus Carota ◽  
Spherical Shape ◽  
Cost Effective ◽  
Surface Plasma Resonance ◽  
Electron Microscopic ◽  
Total Antioxidant ◽  
Average Size ◽  
Quercus Infectoria

Plant mediated fabrication of nanoparticles and nanomaterials are gaining momentum as it is eco-friendly and cost-effective. In the present study, we synthesis of Silver nanoparticles using aqueous extract of  Quercus infectoria nuts and Daucus carota subsp sativum leaves. The surface plasma resonance at 417 and 450 nm for Q. infectoria and D. carota respectively confirmed the formation of AgNPs. Scanning Electron Microscopic (SEM) confirmed the spherical shape of the nanoparticles, which had an average size of 67.5 nm and 49.2 nm for Q. infectoria nanoparticles (QAgNPs)and D. carota nanoparticles (DAgNPs). The elemental composition by Energy-Dispersive X-ray analysis of the nanoparticle showed an atomic percentage of silver as 73.64 % and 75.93% for Q. infectoria and D. carota.FT- IR analysis of the plant extracts and synthesized silver nanoparticles showed the presence of various functional groups. The total antioxidant activity of QAgNPs was 81.18% and that of DAgNPs was 73.36%. The QAgNPs and DAgNPs exhibited antibacterial activity against B. subtilis, E. coli and S. aureus. The percentage of cell viability for QAgNPs and DAgNPs assessed using HeLa cells was 21.1% and 6% respectively.

scholarly journals Green Synthesis, Characterization, and Antibacterial Activity of Silver/Polystyrene Nanocomposite

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Manal A. Awad ◽  
W. K. Mekhamer ◽  
Nada M. Merghani ◽  
Awatif A. Hendi ◽  
Khalid M. O. Ortashi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Food Packaging ◽  
Orange Peel ◽  
Petri Dish ◽  
Cost Effective ◽  
Polymer Chains ◽  
Transmission Electron ◽  
Average Size

A novel, nontoxic, simple, cost-effective and ecofriendly technique was used to synthesize green silver nanoparticles (AgNPs). The AgNPs were synthesized using orange peel extract as a reducing agent for silver nitrate salt (AgNO3). The particle size distribution of AgNPs was determined by Dynamic Light Scattering (DLS). The average size of silver nanoparticles was 98.43 nm. The stable dispersion of silver nanoparticles was added slowly to polystyrene solution in toluene maintaining the temperature at 70°C. The AgNPs/polystyrene (PS) nanocomposite solution was cast in a petri dish. The silver nanoparticles encapsulated within polymer chains were characterized by X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM) equipped with Energy Dispersive Spectroscopy (EDS) in addition to Transmission Electron Microscopy (TEM). The green AgNPs/PS nanocomposite film exhibited antimicrobial activity against Gram-negative bacteriaEscherichia coli, Klebsiella pneumoniae and Salmonella, and Gram-positive bacteriaStaphylococcus aureus. Thus, the key findings of the work include the use of a safe and simple AgNPs/PS nanocomposite which had a marked antibacterial activity which has a potential application in food packaging.

scholarly journals Green Sonochemical Synthesis of Silver Nanoparticles Using Adansonia Digitata Leaves Extract and Evaluation of Their Antibacterial Potential

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Wilson Mbiti Njue ◽  
Jackson Kilonzo Kithokoi ◽  
Jane Mburu ◽  
Henry Mwangi ◽  
Sauda Swaleh
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Metal Nanoparticles ◽  
Pathogenic Bacteria ◽  
Cost Effective ◽  
Adansonia Digitata ◽  
Gram Negative ◽  
Selected Area Electron Diffraction ◽  
Average Size

Metal nanoparticles in the field of nanotechnology are of great interest to modern scientific research due to their size effects, medical uses and, catalytic, electronic and optical properties. Green synthesis of metal nanoparticles is a feasible alternative to chemical methods as it is environmentally friendly and cost effective. In continuation with our research on green synthesis of silver nanoparticles using Kenyan medicinal plants, we here report the synthesis of novel silver nanoparticles (AgNPs) on ultrasonic bath using Adansonia digitata leaves extracts and analysis of their antibacterial activity. The nanoparticles were characterized by UV-Vis, High Resolution Transmission Electron Microscopy (HRTEM), FTIR spectroscopy and Energy Dispersive X-ray (EDX). EDX analysis affirmed the nanoparticles were pure silver. Crystalline nature of the nanoparticles was confirmed by bright circular spots in the Selected Area Electron Diffraction (SAED) in HRTEM image. The AgNPs were spherical with an average size 13 nm. FTIR analysis showed strong –C=C- and –OH stretching bands due to compounds capping the nanoparticles. The synthesized AgNPs showed high inhibition zones of 17.1±0.130 mm towards Gram-negative bacteria E. coli and 12.9±0.082mm towards Gram positive bacteria S. aureus. The aqueous A. digitata extract had no effect on growth inhibition of test bacteria. The study showed that the silver nanoparticles synthesized from the plant’s leaves extract had antibacterial activity against both Gram negative and positive pathogenic bacteria. The nanoparticles can be utilized towards developing novel drugs useful in combating pathogens.

scholarly journals Green Synthesized Silver Nanoparticles Immobilized on Activated Carbon Nanoparticles: Antibacterial Activity Enhancement Study and Its Application on Textiles Fabrics

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3790
Author(s):  
Pratama Jujur Wibawa ◽  
Muhammad Nur ◽  
Mukhammad Asy’ari ◽  
Wijanarka Wijanarka ◽  
Heru Susanto ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Activated Carbon ◽  
Aloe Vera ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
E Coli ◽  
Transmission Electron ◽  
Average Size ◽  
Green Synthesized Silver Nanoparticles

This research aimed to enhance the antibacterial activity of silver nanoparticles (AgNPs) synthesized from silver nitrate (AgNO3) using aloe vera extract. It was performed by means of incorporating AgNPs on an activated carbon nanoparticle (ACNPs) under ultrasonic agitation (40 kHz, 2 × 50 watt) for 30 min in an aqueous colloidal medium. The successful AgNPs synthesis was clarified with both Ultraviolet-Visible (UV-Vis) and Fourier Transform Infrared (FTIR) spectrophotometers. The successful AgNPs–ACNPs incorporation and its particle size analysis was performed using Transmission Electron Microscope (TEM). The brown color suspension generation and UV-Vis’s spectra maximum wavelength at around 480 nm confirmed the existence of AgNPs. The particle sizes of the produced AgNPs were about 5 to 10 nm in the majority number, which collectively surrounded the aloe vera extract secondary metabolites formed core-shell like nanostructure of 8.20 ± 2.05 nm in average size, while ACNPs themselves were about 20.10 ± 1.52 nm in average size formed particles cluster, and 48.00 ± 8.37 nm in average size as stacking of other particles. The antibacterial activity of the synthesized AgNPs and AgNPs-immobilized ACNPs was 57.58% and 63.64%, respectively (for E. coli); 61.25%, and 93.49%, respectively (for S. aureus). In addition, when the AgNPs-immobilized ACNPs material was coated on the cotton and polyester fabrics, the antibacterial activity of the materials changed, becoming 19.23% (cotton; E. coli), 31.73% (polyester; E. coli), 13.36% (cotton; S. aureus), 21.15% (polyester; S. aureus).

scholarly journals Antibacterial Activity of Silver Nanoparticles Synthesized by Bark Extract of Syzygium cumini

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ram Prasad ◽  
Vyshnava Satyanarayana Swamy
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Cost Effective ◽  
Diffusion Method ◽  
Bark Extract ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cost Effective Method ◽  
Uv Visible ◽  
Major Attention

The unique property of the silver nanoparticles having the antimicrobial activity drags the major attention towards the present nanotechnology. The environmentally nontoxic, ecofriendly, and cost-effective method that has been developed for the synthesis of silver nanoparticles using plant extracts creates the major research interest in the field of nanobiotechnology. The synthesized silver nanoparticles have been characterized by the UV-visible spectroscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Further, the antibacterial activity of silver nanoparticles was evaluated by well diffusion method, and it was found that the biogenic silver nanoparticles have antibacterial activity against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853), Azotobacter chroococcum WR 9, and Bacillus licheniformis (MTCC 9555).

scholarly journals Green Synthesis of Silver Nanoparticles from the Extracts of Fruit Peel of Citrus tangerina, Citrus sinensis, and Citrus limon for Antibacterial Activities

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Moira Carmalita Dharsika Niluxsshun ◽  
Koneswaran Masilamani ◽  
Umaramani Mathiventhan
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Citrus Sinensis ◽  
Orange Peel ◽  
Cost Effective ◽  
Diffusion Method ◽  
Citrus Limon ◽  
Fruit Peel ◽  
Visible Absorption ◽  
Uv Visible

Wide application of nanoparticles motivates the need for synthesising them. Here, a nontoxic, eco-friendly, and cost-effective method has been established for the synthesis of silver nanoparticles using extracts of lemon peel (Citrus limon), green orange peel (Citrus sinensis), and orange peel (Citrus tangerina). The synthesised nanoparticles have been characterised using UV-visible absorptionspectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy (TEM). The UV-visible absorption spectrum of these synthesised silver nanoparticles shows an absorption peak at around 440 nm. TEM images show different shaped particles with various sizes. Furthermore, the antibacterial activity of silver nanoparticles was appraised by a well-diffusion method and it was observed that the green synthesised silver nanoparticles have an effective antibacterial activity against Escherichia coli and Staphylococcus aureus. The outcome of this study could be beneficial for nanotechnology-based biomedical applications.

Rapid and Clean Biomimetic Synthesis of Bimetallic Au-Ag Nanoparticles Using an otherwise Worthless and Noxious Weed Ipomoea (Ipomoea carnea)

2015 ◽  
Vol 31 ◽  
pp. 1-14 ◽  
Author(s):  
Sami Ullah Ganaie ◽  
Sruthi Ravindran ◽  
Tasneem Abbasi ◽  
Shahid Abbas Abbasi
Keyword(s):  
Ag Nanoparticles ◽  
Cost Effective ◽  
Biomimetic Synthesis ◽  
One Pot ◽  
X Ray ◽  
Stabilizing Agents ◽  
Ambient Temperatures ◽  
Molar Ratios ◽  
Noxious Weed ◽  
Average Size

In a first report of its type, gainful utilization of the obnoxious weed ipomoea (Ipomoeacarnea;also known as:I.fistulosa) has been achieved by developing a procedure on its basis for clean-green one pot synthesis of bimetallic Au-Ag nanoparticles. In it the leaf and the stem extracts of the weed serve as reducing as well as stabilizing agents. With the support of Scanning Electron Microscopy, Confocal Raman Spectroscopy, and X-ray based techniques, the effect of varying metal: extract stoichiometry, temperature, and stirring on controlling the shape and size of the nanoparticles has been studied. Increase in reaction temperature is seen to favour speedier formation of nanoparticles, and of smaller average size, than occurs at ambient temperatures (27±2°C). Higher extract: metal ratios also lead to nanoparticles of larger average size. When Ag (I) and Au (III) salts are used in equal molar ratios, it generates sphere-shaped nanoparticles. All-in-all, the present work offers a non-polluting, energy saving, and cost effective route for the fabrication of bimetallic Au-Ag nanoparticles. The study indirectly provides a means of controlling ipomoea, thereby offering a means to reduce the ecological degradation that is caused by the weed.

scholarly journals Enhanced antibacterial activity through silver nanoparticles deposited onto carboxylated graphene oxide surface

2021 ◽  
Author(s):  
Arturo Barjola ◽  
María Ángeles Tormo ◽  
Oscar Sahuquillo ◽  
Patricia Bernabé ◽  
José Manuel Pérez ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Biofilm Formation ◽  
Material Surface ◽  
Oxide Surface ◽  
Main Role ◽  
Carboxylic Groups ◽  
Average Size

Abstract The strong bactericidal action of silver nanoparticles (AgNPs) is usually limited for their degree of aggregation. Deposition of AgNPs onto a graphene oxide (GO) surface to generate GO-Ag hybrids has been shown to be an effective method to control these aggregation problems. In this sense, a novel carboxylated graphene oxide-silver nanoparticle (GOCOOH-Ag) material has been synthesized and their antibacterial and biofilm formation inhibition have been studied.AgNPs decorating the GOCOOH surface achieved an average size of 6.74±0.25 nm, which was smaller than those of AgNPs deposited onto the GO surface. In addition, better distribution of AgNPs was obtained using carboxylated material. It is important to highlight the main role of the carboxylic groups in the nucleation and growth of the AgNPs that decorate the GO-based material surface.In vitro antibacterial activity and antibiofilm-forming action were tested against Gram-positive (Staphylococcus aureus and Staphylococcus epidermidis) and Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). Both GO-Ag and GOCOOH-Ag reduced the bacterial growth, analyzed by time-kill curves. However, the minimum inhibitory concentration and the minimum bactericidal concentration of GOCOOH-Ag were lower than those of GO-Ag for all strains studied, indicating that GOCOOH-Ag has better antibacterial activity. In addition, both nanomaterials prevent biofilm-formation, with a higher reduction of biofilm mass and cell viability in the presence of GOCOOH-Ag. The carboxylation functionalization in GO-based materials can be applied to improve the bactericidal and antibiofilm-forming action of the AgNPs.

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