scholarly journals In Vitro Immunomodulatory Activity of Aqueous Extract of Moringa oleifera Lam. Leaf to the CD4 +, CD8+ and B220+ Cells in Mus musculus

2014 ◽  
Vol 4 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Indriya Rachmawati ◽  
◽  
Muhaimin Rifa’i
Keyword(s):  
Aqueous Extract ◽  
Mus Musculus ◽  
Moringa Oleifera ◽  
Immunomodulatory Activity ◽  
Moringa Oleifera Lam

scholarly journals Antioxidant and antiproliferative properties of Moringa oleifera Lam. leaf aqueous extract

2020 ◽  
Vol 7 (4) ◽  
Author(s):  
D Athira Nair ◽  
T J James ◽  
S L Sreelatha ◽  
Bibu John Kariyil
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Moringa Oleifera ◽  
Dependent Manner ◽  
Standard Drug ◽  
In Vivo Analysis ◽  
Moringa Oleifera Lam

Moringa oleifera Lam. is a highly valued medicinal plant in India, especially Kerala. In the present study, antioxidant activity of aqueous extract of leaves of M. oleifera was determined both in-vitro and in-vivo. Male Wistar rats of 3 age groups- 6, 12, and 18 months old were used for in-vivo analysis. In vitro anti-proliferative effect of the extract was carried out in Dalton’s Lymphoma Ascites (DLA) Cells. LCMS-QTOF analysis of the extract was also done to determine the bioactive components present in the extract. Antioxidant activity of M. oleifera leaf showed an IC 50 value of 10.47 ?g/ml and whereas for standard drug, ascorbic acid, it was 19.52 ?g/ml. In-vivo analysis of lipid peroxidation showed a significant reduction of lipid peroxidation in the brains of 12 and 18-months old treated groups. Up to 75% mortality of DLA cancerous cells was observed in-vitro in different concentrations of M. oleifera leaf water extract in a dose-dependent manner, demonstrating its anti-proliferative property. LCMS-QTOF analysis revealed the presence of emodin-8-glucoside in the extract. Molecular docking analysis (Auto Dock Vina) of emodin-8-glucoside with six cancer related proteins showed highest binding affinity with AKT-1 with a binding score of -10.4 kcal/mol, also showed good affinity with NF-kB (p65), Stat-3, Bcl-2, Bcl-xl and c-FLIP. This study helps to choose healthy diet practices to overcome free radical onslaught and cancerous cell proliferation especially in the later stages of life. This can also pave way for the emergence of diet based therapeutic cure for cancer.

scholarly journals EFFECT OF COMBINATION OF AQUEOUS LEAF EXTRACTS OF PSIDIUM GUAJAVA LINN AND MORINGA OLEIFERA LAM ON DIABETES MELLITUS

2020 ◽  
pp. 79-82
Author(s):  
MATHEW ANU JAYAMOL ◽  
REJI ASHLEY ◽  
JOHNS NITHYA ◽  
SHAMSUDEEN SHIJINA
Keyword(s):  
Aqueous Extract ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Moringa Oleifera ◽  
Psidium Guajava ◽  
Leaf Extracts ◽  
Ic50 Value ◽  
Glucose Diffusion ◽  
Moringa Oleifera Lam

Objective: To determine the anti-diabetic activity of combined aqueous extracts (1:1mixture) of dry leaves of Psidium guajava linn and Moringa oleifera lam as well as to compare the anti-diabetic activity of these plants by in vitro methods. Methods: In vitro alpha amylase inhibitory assay was performed on porcine alpha amylase and the absorbance was measured at 540 nm using a microplate reader and glucose diffusion inhibitory assay using dialysis membrane. Acarbose was used as the standard in the above mentioned methods. Results: The mixture (1:1) of aqueous plant extracts (at a concentration of 100µg/ml) of Psidium guajava linn and Moringa oleifera lam exhibited 72.08333% inhibition with IC50 value of 10.9µg/ml. The leaf extracts of Psidium guajava (at a concentration 100µg/ml) exhibited 71.23288% of a α amylase inhibitory activity with an IC50 values 19.883µg/ml whereas the leaf extracts of Moringa oleifera (at a concentration of 100µg/ml) exhibited 70.58824% of α amylase inhibitory activity with an IC50 value of 27.974 µg/ml. The Acarbose (standard drug) at a concentration of 100µg/ml showed 72.09302% inhibitory effect on the α amylase activity with an IC50 value 8.9µg/ml. In glucose diffusion inhibition assay the mixture of plant extracts exhibited 76.57% inhibition at 150 min which produces more effects than the two plants. The aqueous extract of Psidium guajava leaves exhibited maximum glucose diffusion inhibition (75.32%) at 150 min as well as Moringa oleifera leaf extract showed the maximum inhibition of 73.70% at the same time interval. For acarbose the percentage was 82.74 at 150 min. The interpretation of the results was done by one-way anova method. Conclusion: The combined extract of the leaves of the 2 plants was found to be more effective than individual plant extracts against diabetes. On comparison of two plants Psidium guajava was found to be more active against diabetes than Moringa oleifera. Also the potentiation effect shown by the combination of extract may be due to synergistic effect of the phytochemical constituents. As the 1:1 mixture of the aqueous extract is found to be more active, the combination of the two plants can be used to formulate drugs for treating diabetes.

Bioaccessibility, antioxidant activity and modulation effect on gut microbiota of bioactive compounds from Moringa oleifera Lam. leaves during digestion and fermentation in vitro

2019 ◽  
Vol 10 (8) ◽  
pp. 5070-5079 ◽  
Author(s):  
Zuman Dou ◽  
Chun Chen ◽  
Xiong Fu
Keyword(s):  
Antioxidant Activity ◽  
Gut Microbiota ◽  
Bioactive Compounds ◽  
Moringa Oleifera ◽  
Modulation Effect ◽  
Gastrointestinal Digestion ◽  
Colonic Fermentation ◽  
Moringa Oleifera Lam

This study aims to investigate the bioaccessibility, bioactivity and gut microbiota modulation effect of Moringa oleifera Lam. leaves after in vitro gastrointestinal digestion and colonic fermentation.

scholarly journals Ekstrak Etanol Moringa Oleifera Lam Terhadap Folikulogenesis Pada Mencit Model Endometriosis

2017 ◽  
Vol 19 (3) ◽  
pp. 246
Author(s):  
Rila Rindi Antina
Keyword(s):  
Mus Musculus ◽  
Moringa Oleifera ◽  
Hematoxylin Eosin ◽  
Moringa Oleifera Lam

Endometriosis didefinisikan sebagai adanya jaringan endometrium di luar rahim.Peningkatan konsentrasi sitokin inflamasi dan faktor pertumbuhan di dalam cairanperitonium berkontribusi pada pembentukan lesi peritonium. Faktor pertumbuhanberperan selama folliculogenesis. Moringa oleifera kaya akan flavonoid. Kuercetin dankaempferol adalah flavonoid, senyawa dengan gugus hidroksil fenolik dengan aksiantioksidan yang memiliki kegunaan potensial terapeutik. Penelitian ini untukmembuktikan adanya pengaruh pemberian ekstrak etanol daun kelor (Moringa oleiferaLam) terhadap folikulogenesis pada mencit (Mus musculus) model endometriosis.Metode penelitian menggunakan mencit (Mus musculus) 20-25g yang dibagi menjadi duakelompok, yaitu kelompok kontrol endometriosis dan kelompok perlakuan diberi ekstraketanol daun kelor 0,35 mg/gBB selama 14 hari. Jaringan ovarium dilakukan pemeriksaanhistopatologi dengan pengecatan hematoxylin-eosin. Hasil penelitian menunjukkanterdapat perbedaan yang signifikan (ρ<0,05) jumlah folikel primer, jumlah folikelsekunder, jumlah folikel tersier, jumlah folikel de graaf pada kelompok kontrol dankelompok perlakuan. Kesimpulan Penelitian menunjukkan ekstrak etanol daun kelormeningkatkan folikulogenesis pada mencit model endometriosisKata kunci—Ekstrak etanol daun kelor, folikulogenesis, endometrosis 

The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves

2008 ◽  
Vol 116 (3) ◽  
pp. 439-446 ◽  
Author(s):  
Pilaipark Chumark ◽  
Panya Khunawat ◽  
Yupin Sanvarinda ◽  
Srichan Phornchirasilp ◽  
Noppawan Phumala Morales ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Ex Vivo ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Moringa Oleifera Lam

scholarly journals Morfometri Ovarium setelah Pemberian Ekstrak Daun Kelor (Moringa oleifera LAM)

2021 ◽  
Vol 9 (1) ◽  
pp. 44-52
Author(s):  
Musthamin Balumbi ◽  
Fachruddin Fachruddin ◽  
Muhammad Risman
Keyword(s):  
Mus Musculus ◽  
Moringa Oleifera ◽  
Moringa Oleifera Lam

Ovarium merupakan organ reproduksi utama dalam sistem reproduksi betina yang berperan dalam menghasilkan sel ovum. Kualitas folikel yang dihasilkan ovarium dipengaruhi oleh nutrisi yang dikonsumsi suatu individu. Kelor (Moringa oleifera LAM) telah lama dikenal sebagai tanaman dengan kandungan nutrisi yang cukup tinggi. Nutrisi yang terdapat di dalam kelor adalah vitamin, mineral, antioksidan, asam amino esensial dan senyawa fitosterol. Kandungan nutrisi pada kelor paling banyak ditemukan di daun. Penelitian ini bertujuan untuk mengetahui kualitas ovarium setelah pemberian ekstrak daun kelor (Moringa oleifera LAM). Metode yang digunakan adalah penelitian eksperimental. Hewan yang digunakan dalam penelitian ini adalah 60 ekor mencit betina (Mus musculus) umur 14-15 minggu dengan rerata bobot badan 30-35 g. Ekstrak daun kelor yang diberikan dengan dosis 0 mg kg-1 BB, 300 mg kg-1 BB, 400 mg kg-1 BB, 500 mg kg-1 BB. Pengamatan dilakukan setelah pemberian 5, 10, 15, 24, dan 34 hari. Parameter penelitian meliputi siklus estrus dan morfometri ovarium. Hasil Penelitian menunjukkan bahwa tingkat konsentrasi dan lamanya pemberian ekstrak daun kelor memberi pengaruh terhadap panjang, lebar, dan bobot ovarium mencit. Konfirmasi siklus estrus bertujuan untuk mengetahui bahwa siklus reproduksi dalam ovarium mencit berlangsung normal. Siklus estrus pada mencit penelitian rata-rata berlangsung normal, yakni terjadi selama 4-5 hari.

scholarly journals Antihelminthic Activity in Aqueous Leaf Extract of Moringa oleifera Lam.

2018 ◽  
Vol 10 (03) ◽  
Author(s):  
Anitha R ◽  
Sahaya Kalaivani
Keyword(s):  
Aqueous Extract ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Condensed Tannin ◽  
Death Time ◽  
Aqueous Leaf Extract ◽  
Round Worm ◽  
Synthetic Drug ◽  
Moringa Oleifera Lam

The objective of the study is to evaluate the antihelminthic property of Moringa oleifera aqueous leaf extract by studying the paralysis and mortality time of Tubiflex tubiflex (blood worm). The aqueous extract at 200mg/ml exhibited paralysis time of 1 minute and death time of 2 minutes which was on par with synthetic drug Albendazole at 20mg/ml. Crude condensed tannin paralysed T.tubiflex at 60 seconds with mortality time of 9 minutes at 1mg/ml. T.tubiflex was selected for the study since they share a similar anatomical and physiological aspects with the intestinal round worm. The study indicated that T.tubiflex was inhibited effectively by condensed tannin in Moringa oleifera aqueous leaf extract and perhaps might be more effective on the round worms as well, since it is consumed as vegetable for its nutritive and medicinal values in tropical and subtropical regions.

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