scholarly journals In vitro studies on antioxidant, ? – amylase and ? – glucosidase inhibitory activities of ethanol extracts of Syzygium cumini seeds

Biomedicine ◽  
2020 ◽  
Vol 39 (2) ◽  
pp. 258-262
Author(s):  
M. Narmatha ◽  
S. Maneemegalai
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Syzygium Cumini ◽  
Total Alkaloids ◽  
Dpph Scavenging Activity ◽  
Inhibitory Activities ◽  
Dpph Scavenging

Introduction and Aim: Diabetes mellitus is a metabolic disorder leads to many secondary complications. The drugs used for treatment causes serious side effects. Syzygium cumini is used in traditional medicine for treating many diseases. The aim of the present study is to estimate phytochemical contents, antioxidant activity, ? – amylase and ? – glucosidase inhibitory activities of ethanol extract of seeds of S. cumini by in vitro methodology. Materials and Methods: The powdered seeds were extracted with ethanol. Quantitative analysis of Total alkaloids, Total phenols, Total flavonoids, Total tannins, Total saponins and Total steroids were carried out. DPPH scavenging activity, ? – amylase and ? – glucosidase inhibitory activities were measured with varying concentration of extract. Results: Total alkaloids content was observed to be higher followed by Total phenol content. Total saponin was found to be present in lesser amount among the tested phytochemicals. 500 µg/ml and above concentrations of seed extract possess above 90% DPPH scavenging activity; 1000 µg/ml concentration of the extract exhibited 43.20% and 19.80% inhibition activity on ? – amylase and ? – glucosidase enzymes. Conclusion: The above results indicate a higher antioxidant activity and appreciable inhibitory activities of enzymes responsible for elevated circulation of glucose. These activities are due to the presence of phytochemicals present in the seeds extract of S. cumini and can be utilized for the management of Diabetes.  

Antioxidant activity of crude ethanolic extract and fractions of Ziziphus mauritiana Lam. (Rhamnaceae) leaves from Burkina Faso

2019 ◽  
Vol 30 (3) ◽  
Author(s):  
Estelle N.H. Youl ◽  
Cyrille A.P. Ouédraogo ◽  
Moustapha Gambo ◽  
Moussa Ouédraogo ◽  
Martin Kiendrebéogo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Burkina Faso ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Ziziphus Mauritiana ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Abstract Background Ziziphus mauritiana Lam. is a plant used in traditional medicine in Burkina Faso in the treatment of several diseases, of which diabetes is characterized by oxidative stress. The aim of this study was to evaluate the in vitro antioxidant potential of the extracts of leaves of this plant. Methods The crude hydroethanolic extract (HEE) of the leaves of Z. mauritiana and their partitionates in n-hexane, dichloromethane, and ethyl acetate, and in the residual aqueous solution (the F1, F2, F3, and F4 fractions, respectively) were first prepared. The content of polyphenols was determined and the antioxidant effects of the extracts were evaluated by their 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, inhibition of lipid peroxidation (TBARS), and the ferric reducing antioxidant power (FRAP). Results The HEE as well as the F3 and F4 fractions were rich in polyphenols with contents between 58 and 84 mg equivalent gallic acid per 100 mg. The flavonoid content was 4 mg quercetin equivalents in the HEE and the F4 fraction. Except for the F1 fraction, the HEE and the other fractions showed significant DPPH scavenging activity (IC50 between 8 and 12 μg/mL). The IC50 of TBARS by different extracts was in the range 1–5 μg/mL, and the FRAP activity was 7–85 mg ascorbic acid equivalent per 100 mg. Total polyphenol content was highly correlated with the antioxidant activities. Conclusions The HEE, F3, and F4 fractions were found to be the richest in polyphenols and had the best antioxidant activity. The antioxidant activity of the extracts of the leaves of Z. mauritiana is due to these polyphenolic compounds.

scholarly journals Optimization of Ultrasonic Cellulase-Assisted Extraction and Antioxidant Activity of Natural Polyphenols from Passion Fruit

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2494
Author(s):  
Wei Wang ◽  
Yu-Ting Gao ◽  
Ji-Wen Wei ◽  
Yin-Feng Chen ◽  
Qing-Lei Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Ph Value ◽  
Soxhlet Extraction ◽  
Passion Fruit ◽  
Total Phenol Content ◽  
Extraction Temperature ◽  
Scavenging Activity ◽  
Solid Ratio ◽  
Dpph Scavenging

In this paper, ultrasonic cellulase extraction (UCE) was applied to extract polyphenols from passion fruit. The extraction conditions for total phenol content (TPC) and antioxidant activity were optimized using response surface methodology (RSM) coupled with a Box-Behnken design (BBD). The results showed that the liquid-to-solid ratio (X2) was the most significant single factor and had a positive effect on all responses. The ANOVA analysis indicated quadratic models fitted well as TPC with R2 = 0.903, DPPH scavenging activity with R2 = 0.979, and ABTS scavenging activity with R2 = 0.981. The optimal extraction parameters of passion fruit were as follows: pH value of 5 at 30 °C for extraction temperature, 50:1 (w/v) liquid-to-solid ratio with extraction time for 47 min, the experimental values were found matched with those predicted. Infrared spectroscopy suggested that the extract contained the structure of polyphenols. Furthermore, three main polyphenols were identified and quantified by HPLC. The results showed the content of phenolic compounds and antioxidant activity of the optimized UCE were 1.5~2 times higher than that determined by the single extraction method and the Soxhlet extraction method, which indicates UCE is a competitive and effective extraction technique for natural passion fruit polyphenols.

scholarly journals Sunflower (Helianthus annuus L.) Plants at Various Growth Stages Subjected to Extraction—Comparison of the Antioxidant Activity and Phenolic Profile

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 535 ◽  
Author(s):  
Francesco Gai ◽  
Magdalena Karamać ◽  
Michał A. Janiak ◽  
Ryszard Amarowicz ◽  
Pier Giorgio Peiretti
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Growth Stages ◽  
Scavenging Activity ◽  
Flowering Stage ◽  
Phenolic Profile ◽  
Aerial Parts ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The aim of this study was to evaluate the differences in the antioxidant activity and phenolic profile of sunflower (Helianthus annuus L.) extracts obtained from the aerial parts of plants harvested at five growth stages. In vitro assays were used to determine the antioxidant activity, i.e., ABTS•+ and DPPH• scavenging activity, the ferric-reducing antioxidant power (FRAP) and the ability to inhibit β-carotene–linoleic acid emulsion oxidation. Phenolic compounds, such as mono- and dicaffeoylquinic acid isomers and caffeic acid hexose, were identified using the LC–TOF–MS/MS technique. The predominant compound during the growth cycle of the plant was 3,5-di-O-caffeoylquinic acid, whose content was the highest at the mid-flowering stage. The total phenolic content was also the highest in sunflowers at the mid-flowering stage. The main phenolic compound contents were closely correlated with ABTS•+ and DPPH• scavenging activity and FRAP. No significant correlation was found between the total phenolic content and the antioxidant activity in the emulsion system. The highest antiradical activity and FRAP were generally determined in older plants (mid-flowering and late flowering stages). In conclusion, the aerial parts of sunflowers, in particular those harvested at the mid-flowering stage, are a good plant material from which to obtain phenolic compound extracts, albeit mainly of one class (esters of caffeic acid and quinic acid), with high antioxidant activity.

scholarly journals Optimization and Identification of Antioxidant Peptide from Underutilized Dunaliella salina Protein: Extraction, In Vitro Gastrointestinal Digestion, and Fractionation

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Enqin Xia ◽  
Lu Zhai ◽  
Zhigang Huang ◽  
Hairong Liang ◽  
Hui Yang ◽  
...  
Keyword(s):  
Dunaliella Salina ◽  
Protein Extraction ◽  
Amino Acid Sequences ◽  
Ultrasound Wave ◽  
Scavenging Activity ◽  
Gastrointestinal Digestion ◽  
Ultrasound Assisted Extraction ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

DPPH• scavenging peptides (<3kDa) from underutilized Dunaliella salina protein were obtained by the following successive treatment, i.e., ultrasound extraction, simulated in vitro gastrointestinal digestion hydrolyzation, and membrane ultrafiltration classification. The optimal condition for ultrasound-assisted extraction was an ultrasound wave with 800 W of power treating a mixture of 60 mL of 1.0 mol L−1 NaOH and 2 g algae powder for 15 min. A high correlation (r=0.8146) between DPPH• scavenging activity and yield of the intact peptides showed their antioxidant capacity. Simulated in vitro digestion assay resulted in excellent DPPH• scavenging activity of the total peptide, amounting to (86.5 ± 10.1)%, comparing with the nondigestion samples at (46.8 ± 6.5)%. After fractionation, the 500-1000 Da fraction exhibited the highest DPPH• scavenging activity (81.2 ± 4.0)%, increasing 1.5 times due to digestion. Then, the 500-1000 Da fraction was analyzed by RPLC-Q Exactive HF mass spectrometer, and 4 novel peptides, i.e., Ile-Leu-Thr-Lys-Ala-Ala-Ile-Glu-Gly-Lys, Ile-Ile-Tyr-Phe-Gln-Gly-Lys, Asn-Asp-Pro-Ser-Thr-Val-Lys, and Thr-Val-Arg-Pro-Pro-Gln-Arg, were identified. From these amino acid sequences, hydrophobic residues accounted for 56%, which indicated their high antioxidant property. The results indicated that underutilized protein of Dunaliella salina could be a potential source of antioxidative peptides through simulated in vitro gastrointestinal digestion.

scholarly journals PHYTOCHEMICAL IDENTIFICATION AND ANTIOXIDANT ACTIVITY OF PASSIFLORA FOETIDA FRUITS AND LEAVES EXTRACTS: A COMPARATIVE STUDY

2020 ◽  
pp. 55-58 ◽  
Author(s):  
YOHANES TANDORO ◽  
PAINI SRI WIDYAWATI ◽  
TARSISIUS DWI WIBAWA BUDIANTA ◽  
GRACE SUMARGO
Keyword(s):  
Antioxidant Activity ◽  
Cardiac Glycosides ◽  
Reducing Power ◽  
Total Phenol ◽  
Total Flavonoids ◽  
Scavenging Activity ◽  
Phytochemical Compounds ◽  
Dpph Scavenging Activity ◽  
Passiflora Foetida ◽  
Dpph Scavenging

Objective: The objective of this study was to compare the phytochemical composition and antioxidant activity of Passiflora foetida fruits and leaves extract. Methods: The parameters observed in this study were phytochemical compounds including alkaloid, flavonoid, phenolic, sterol, triterpenoid, saponin, tannin, and cardiac glycoside, total phenolic content Folin Ciocalteu method is based on reduction of Folin Ciocalteu reagent in alkaline medium; the metal complex produced measured at λmax: 760 nm; total flavonoids content with AlCl3 Colorimetric method based on complex formation of AlCl3 and flavonoid content in alkaline medium, the AlCl3-flavonoid complex produced measured at λmax: 510 nm; free radical DPPH scavenging activity; and ferric reducing power based on reduction of Fe3+ion into Fe2+ion that reacted with FeCl3 to form a ferric-ferrous complex that measured at λmax: 700 nm. Results: Passiflora leaves extract has phytochemical compound such as alkaloids, phenolics, flavonoids, saponins, and cardiac glycosides, total phenol was 22.92±0.18 mg GAE/g sample dry base, total flavonoid was 7.01±0.10 mg CE/g sample dry base, DPPH scavenging activity was 2.77±0.02 mg GAE/g sample dry base and ferric reducing power was 3.20±0.04 mg GAE/g sample dry base meanwhile Passiflora fruits extract had phytochemical compounds such as alkaloid, phenolic, flavonoids, cardiac glycosides, total phenol was 6.53±1.02 mg GAE/g sample dry base, total flavonoids were 1.56±0.27 mg CE/g sample dry base, DPPH free radical scavenging activity was 1.00±0.15 mg GAE/g sample dry base, and ferric reducing power was 1.12±0.17 mg GAE/g sample dry base. Conclusion: Passiflora leaves extract has higher total phenol, total flavonoid and antioxidant activity measured by DPPH scavenging activity and ferric reducing power value compared with Passiflora fruits extract.

scholarly journals Optimization of the Extraction and Stability of Antioxidative Peptides from Mackerel (Pneumatophorus japonicus) Protein

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Xueqin Wang ◽  
Huahua Yu ◽  
Ronge Xing ◽  
Xiaolin Chen ◽  
Song Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protein Hydrolysate ◽  
Scientific Basis ◽  
Enzyme Concentration ◽  
Scavenging Activity ◽  
Preparation Conditions ◽  
Low Concentrations ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
The Stability

This study optimizes the preparation conditions for mackerel protein hydrolysate (MPH) by response surface methodology (RSM) and investigates the stability of the antioxidant activity of MPHs (<2.5 kDa). The optimal conditions were as follows: enzyme concentration of 1726.85 U/g, pH of 7.00, temperature of 39.55°C, time of 5.5 h, and water/material ratio of 25 : 1, and the maximum DPPH scavenging activity was 79.14%. The MPHs indicated significant cellular antioxidant activity at low concentrations. Furthermore, the temperature and freeze-thaw cycles had little effect on the antioxidative stability while pH had significant effect on the antioxidative stability. In addition, the MPHs were sensitive to the metal ions, such as Fe2+, Fe3+, Zn2+, and Cu2+. Notably, when the concentrations of Fe2+and Fe3+were 5 mM, the DPPH scavenging activities were only 1.1% and 0.6%, respectively; furthermore, Cu2+at a 5 mM concentration could completely inhibit the DPPH scavenging activity of MPHs. In contrast, K+and Mg2+had no notable effect on the antioxidant activity of MPHs. These results may provide a scientific basis for the processing and application of MPHs.

Antioxidant profiling of Hippophae salicifolia growing in sacred forests of Sikkim, India

2011 ◽  
Vol 38 (9) ◽  
pp. 697 ◽  
Author(s):  
Arvind K. Goyal ◽  
Bharat C. Basistha ◽  
Arnab Sen ◽  
Sushil K. Middha
Keyword(s):  
Antioxidant Activity ◽  
Sea Buckthorn ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Methanol Extracts ◽  
Hippophae Salicifolia ◽  
Total Antioxidant ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Phenolic And Flavonoid

Hippophae commonly known as sea-buckthorn, is a versatile plant with multipurpose uses including the control of soil erosion, biological nitrogen fixation and medicinal properties. In India, two species of sea-buckthorn; namely, Hippophae salicifolia D. Don and Hippophae rhamnoides L., are very common. H. salicifolia has a shrub-to-tree habit and is restricted to the Himalayan region, whereas H. rhamnoides is bushy, growing at higher altitude in India, and widely distributed in Europe and Asia. In this study, aqueous, methanol and acetone extracts of various parts of female and male plants of H. salicifolia were evaluated for total antioxidant activity, phenolic and flavonoid contents, and correlations were made. The total phenolic and flavonoid content in all the samples ranged from 99 ± 32 to 1459 ± 53 mg gallic acid equivalent g–1, 135 ± 12 to 707 ± 62 mg quercetin equivalent g–1 of dry extract, respectively. The highest free radical (DPPH) scavenging activity was found in methanol extracts of female bark and male leaf. Methanol extracts of male bark and male leaf showed maximum H2O2 activity at a concentration of 1 mg mL–1. Antioxidant activity (FRAP method) ranged from 0.021 to 1.041 at concentrations ranging from 20 to 200 µg mL–1 with a reducing capacity of extracts as water > methanol > acetone. Strong correlation (P < 0.05) was observed between DPPH scavenging activity and total phenolic content from all the extracts under study.

scholarly journals Chemical Constituents Antioxidant and Anticholinesterasic Activity ofTabernaemontana catharinensis

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Carla Nicola ◽  
Mirian Salvador ◽  
Adriana Escalona Gower ◽  
Sidnei Moura ◽  
Sergio Echeverrigaray
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Neurodegenerative Diseases ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Alkaloid Content ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The present work aimed to analyze the alkaloid content of the ethanolic extract ofTabernaemontana catharinensis(Apocynaceae family) and its fractions as well as to evaluate their antioxidant and anticholinesterasic activities. The analyses of the ethanolic extract ofT. catharinensisby mass spectrometry allowed identifying the presence of the alkaloids 16-epi-affinine, coronaridine-hydroxyindolenine, voachalotine, voacristine-hydroxyindolenine, and 12-methoxy-n-methyl-voachalotine, as well as an alkaloid withm/z385.21 whose spectrum suggests a derivative of voacristine or voacangine. The extract and its alkaloid rich fractions showed antioxidant activity, especially those that contain the alkaloidm/z385.21 or 16-epi-affinine with DPPH scavenging activity (IC50) between 37.18 and 74.69 μg/mL. Moreover, the extract and its fractions exhibited anticholinesterasic activity, particularly the fractions characterized by the presence of 12-methoxy-n-methyl-voachalotine, with IC50= 2.1 to 2.5 μg/mL. Fractions with 16-epi-affinine combined good antioxidant (IC50= 65.59 to 74.69 μg/mL) and anticholinesterasic (IC50= 7.7 to 8.3 μg/mL) activities, representing an option for further studies aimed at treating neurodegenerative diseases.

Preparation and characterization of phloretin by complexation with cyclodextrins

2020 ◽  
Vol 44 (14) ◽  
pp. 5218-5223
Author(s):  
Xiaona Hu ◽  
Ziruo Zhou ◽  
Lijun Han ◽  
Shujing Li ◽  
Wei Zhou
Keyword(s):  
Antioxidant Activity ◽  
Inclusion Complexes ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

RP and DPPH scavenging activity tests revealed that the antioxidant activity of phloretin improved after the formation of inclusion complexes.

scholarly journals Formulation and Evaluation of Mangosteen (Garcinia mangostana L.) Fruit Pericarp Extract Gel

2021 ◽  
Vol 11 (2) ◽  
pp. 90-97
Author(s):  
Rina Kuswahyuning ◽  
Indra Lesmana
Keyword(s):  
Skin Disorder ◽  
In Vitro Study ◽  
Scavenging Activity ◽  
Garcinia Mangostana ◽  
Topical Gel ◽  
Skin Delivery ◽  
Dpph Scavenging Activity ◽  
Fruit Pericarp ◽  
Dpph Scavenging

The topical antioxidant product may be useful for the treatment of oxidative stress-related skin disorder. This research aimed to evaluate a topical gel formulation of Garcinia mangostana L. fruit pericarp (GMP) extract. GMP extract was formulated into a gel and characterized for its physical properties. The antioxidant activity was evaluated based on the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. The ability of gel formulation to release GMP extract and promote skin delivery was estimated based on DPPH scavenging method and also compared to that of suspension form. The results showed that the GMP extract gel showed characteristics for topical application. The radical DPPH scavenging activity was confirmed both in GMP extract and GMP extract gel. In vitro study release showed that the GMP extract was released from gel. Some degree of GMP extract was also delivered into the shed snakeskin in vitro. When compared with the suspension form, GMP extract gel provided a more profoundly lower release.

Sign in / Sign up

Export Citation Format

Share Document