Effect of Ocimum sanctum and Allium sativumon Lipid Peroxidation and Antioxidant Enzymes in Alloxan Induced Diabetic Rats

2020 ◽  
Vol 13 (2) ◽  
pp. 882-888
Author(s):  
Shiv Kumar Jayant
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Diabetic Rats ◽  
Ocimum Sanctum

scholarly journals Antioxidant Responses of Arsenite-induced Oxidative Stress in Rice (Oryza sativa L.) and its Modulation by Eugenol (extracted from Ocimum sanctum)

2021 ◽  
Author(s):  
Samya Mairaj ◽  
Richa Dave Nagar ◽  
Lakshmikant Bhardwaj ◽  
F. Rehman ◽  
Anirudh Punnakal ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Oryza Sativa L ◽  
Arsenic Exposure ◽  
Substantial Reduction ◽  
Antioxidant Defense System ◽  
Ocimum Sanctum ◽  
Rice Seedlings ◽  
Arsenic Stress

Background: Irrigation with arsenic-contaminated groundwater is leading to high arsenic-laden rice seeds and lower yields. In the present study, the effect of exogenous treatment of eugenol (extracted from Ocimum sanctum L leaf) on hydroponically grown rice seedlings was examined by investigating the antioxidant system under arsenic stress. Methods: In the experiment 7 day old rice seedlings (IR-64) were exposed to 10,50,100 µM of arsenite separately and co-treatment with 10,50,100 µM eugenol in a hydroponic medium for 7 days. The activity of antioxidant enzymes such as superoxide dismutase, ascorbate peroxidase, glutathione peroxidase, catalase and lipid peroxidation (malondialdehyde) in root and shoot tissues were determined separately by standard protocol. Result: Under arsenic treatment oxidative stress was induced by overproduction of reactive oxygen species (ROS) and disruption of antioxidant defense system measured in terms of increased activity of antioxidant enzymes and lipid peroxidation (malondialdehyde) in root and shoot tissues separately. Eugenol-treated seedlings along with arsenic exposure substantially decreased the level of arsenic uptake in plants resulting in a substantial reduction in ROS overproduction and MDA content. SOD, CAT, GPX activities perform an influential role in arsenic stress acclimatization and eugenol treated seedlings with arsenic exposures indicated substantial changes in all variables evaluated as compared to arsenic treatment only. This study suggests that oxidative stress caused by arsenic was ameliorated by eugenol.

The protective effect of olive cake treatment on oxidant/antioxidant biomarkers, on serum, red blood cells and liver, in streptozotocin-induced diabetic rats fed cholesterol-enriched diet

2019 ◽  
Vol 50 (4) ◽  
pp. 785-798
Author(s):  
Yahiaoui Zidan ◽  
Sherazede Bouderbala ◽  
Cherrad Hayet ◽  
Bouchenak Malika
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Diabetic Rats ◽  
Male Rats ◽  
Olive Cake ◽  
Content Type ◽  
Enzymes Activities ◽  
Antioxidant Enzymes Activities

Purpose The purpose of this study is to determine the effect of olive cake (OC) on lipid peroxidation as well as antioxidant enzymes activities of serum, red blood cells (RBCs) and liver, in streptozotocin (STZ)-induced-diabetic rat fed cholesterol-enriched diet. Design/methodology/approach Hypercholesterolemic male rats were rendered diabetic (HC-D) by a single intraperitoneal injection dose of STZ (35 mg/kg BW). HC-D rats were divided into two groups fed for 28d a diet supplemented with OC at 7.5 percent (HC-D-OC) or not (HC-D). A control group (C) was submitted to standard diet containing 20 per cent casein for the same experimental period. Findings RBCs, serum and liver thiobarbituric acid reactive substances (TBARS) contents were significantly increased in HC-D, compared to C group (p = 0.04, p = 0.02 and 0.03). These values were significantly decreased (48 per cent and 64 per cent; p = 0.02 and p = 0.0007) in serum and liver of HC-D-OC vs HC-D group. In RBCs, superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) activities were, respectively, 1.5, 2- and 1.7-fold higher (p = 0.03, p = 0.008 and p = 0.03) in HC-D group compared to HC group. In serum and liver, SOD, CAT and GST activities were, respectively, 1.3-, 2.6- and 1.6-fold increased (p = 0.03, p = 0.007 and p = 0.02). In HC-D-OC compared to HC-D group, RBCs glutathione peroxidase (GSH-Px), CAT and GST activities were, respectively, 2.1-, 3.3- and 2.1-fold higher (p = 0.04, p = 0.0009 and p = 0.03). In serum, SOD and CAT activities were, respectively, 1.5- and 1.9-fold increased (p = 0.02, p = 0.02). In liver, SOD, GSH-PX, CAT and GST activities were significantly increased (p = 0.005, p = 0.03, p = 0.02 and p = 0.04). Originality/value In diabetic rats-fed cholesterol-enriched diet, OC was able to reduce oxidative stress by decreasing lipid peroxidation and increasing antioxidant enzymes activities in serum, RBCs and liver.

scholarly journals Antidiabetic and Neuroprotective Effects of Trigonella Foenum-graecum Seed Powder in Diabetic Rat Brain

2012 ◽  
Vol 113 (1) ◽  
pp. 33-43 ◽  
Author(s):  
P. Kumar ◽  
R. K. Kale ◽  
P. McLean ◽  
Najma Zaheer Baquer
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Rat Brain ◽  
Membrane Fluidity ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Neuroprotective Effects ◽  
Trigonella Foenum Graecum ◽  
Chronic Hyperglycemia ◽  
Diabetic Brain

Trigonella foenum-graecum seed powder (TSP) has been reported to have hypoglycemic and hyperinsulinemic action. The objective of the study was to examine the antidiabetic and neuroprotective role of TSP in hyperglycemiainduced alterations in blood glucose, insulin levels and activities of membrane linked enzymes (Na+K+ATPase, Ca2+ATPase), antioxidant enzymes (superoxide dismutase, glutathione S-transferase), calcium (Ca2+) levels, lipid peroxidation, membrane fluidity and neurolipofuscin accumulation in the diabetic rat brain. Female Wistar rats weighing between 180 and 220 g were made diabetic by a single injection of alloxan monohydrate (15 mg/100 g body weight), diabetic rats were given 2 IU insulin, per day with 5% TSP in the diet for three weeks. A significant increase in lipid peroxidation was observed in diabetic brain. The increased lipid peroxidation following chronic hyperglycemia was accompanied with a significant increase in the neurolipofuscin deposition and Ca2+ levels with decreased activities of membrane linked ATPases and antioxidant enzymes in diabetic brain. A decrease in synaptosomal membrane fluidity may influence the activity of membrane linked enzymes in diabetes. The present study showed that TSP treatment can reverse the hyperglycemia induced changes to normal levels in diabetic rat brain. TSP administration amended effect of hyperglycemia on alterations in lipid peroxidation, restoring membrane fluidity, activities of membrane bound and antioxidant enzymes, thereby ameliorating the diabetic complications.

scholarly journals The Aerobic Training and Berberine Chloride Intervention on Pancreatic Tissue Antioxidant Enzymes and Lipid Peroxidation in Type 1 Diabetic Rats

2020 ◽  
Author(s):  
Javad Ramezani ◽  
Mohammad Ali Azarbayjani ◽  
Maghsoud Peeri
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Aerobic Training ◽  
Diabetic Control ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Male Rats ◽  
P Value ◽  
Berberine Chloride

Objective: Aerobic training and berberine chloride include antioxidant characteristics. In this study, aerobic training and berberine chloride intervention on the activity of antioxidant enzymes and lipid peroxidation of pancreatic tissue were investigated in type 1 diabetic male wistar rats. Materials and Methods: In the current study, 56 Wistar male rats were randomly assigned to seven different groups (n= 8), these groups include healthy control, diabetic control, Berberine-treated diabetes (15&30 mg/kg), aerobic training-treated diabetes, Diabetes treated with Berberine and aerobic training (15&30 mg/kg). The aerobic training schedule consisted of 6 weeks treadmills with a frequency of 5 sessions per week. The Berberine was also fed a specific dose every day and a half before training. The superoxide dismutase, glutathione peroxidase, catalase, and malondialdehyde were assessed using ELISA method. Results: The results showed that aerobic training, as well as the intervention of Aerobic Training and Berberine chloride, had a significant effect on the increase of antioxidant enzymes SOD and CAT in the pancreatic tissue groups (P-value< 0.05), but did not significantly affect the GPX level (P-value> 0.05). There was a significant decrease in MDA level in all treatment groups (P-value< 0.05). In diabetic groups that received both treatments at the same time, the MDA level more decreased (P-value< 0.0005). Conclusion: The aerobic training and berberine chloride concurrent intervention have a greater effect on the antioxidant enzymes in the pancreatic tissue of diabetic specimens. Therefore, it is recommended that aerobic training be done with berberine chloride.

scholarly journals Protective Potential of Azadirachta indica Leaf Extract in Diabetic Rat Liver

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Gupta N.K. ◽  
Srivastva N. ◽  
Bubber P. ◽  
Garg S. ◽  
Mohammad O.
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Azadirachta Indica ◽  
Liver Tissue ◽  
Major Complication ◽  
Tissue Level ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Leaf Extracts ◽  
Glucose Levels

Diabetes mellitus is a debilitating metabolic disorder and emerged as a major complication nowadays. The role of plants and their derivatives is indispensable in the treatment of various diseases. Azadirachta indica is a medicinal plant and holds a potential to attenuate the pathological changes associated with diabetes. In the present study streptozotocin (STZ) induced diabetic rats received the Azadirachta indica leaf extracts (ALE) treatment for a period of seven consecutive days (600 mg/Kg body weight) and then evaluated for changes in the liver tissue. The diabetic rats exhibited significant (p≤0.001) increase in the lipid peroxidation levels, decrease in GSH levels and modulation in the activities of various antioxidant enzymes including catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase. However, following ALE treatment to diabetic rats reported decrease in the blood glucose levels, lipid peroxidation levels, improved GSH levels and restored the activities of various antioxidant enzymes towards control levels. Besides this, light microscopic and ultramicroscopic examination of liver tissue also demonstrated less fragmentation, inflammation and decreased tissue level damage after ALE treatment, and corroborated the results of biochemical parameters. Thus, the results of present study revealed the hepatoprotective potential of Azadirachta indica in diabetes

Lipid peroxidation and activity of antioxidant enzymes in diabetic rats

1995 ◽  
Vol 151 (2) ◽  
pp. 113-119 ◽  
Author(s):  
Rakesh Kakkar ◽  
Jawahar Kalra ◽  
Subrahmanyam V. Mantha ◽  
Kailash Prasad
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Diabetic Rats
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