Screening and Evaluation of a Potential Bacteriocin Producing Lactobacillus in Milk and Dairy Products Collected from Puducherry

In an array of identifying safe antimicrobial compounds, bacteriocin producing Lactobacillus strain have been investigated in this study from the daily consuming food resources of humans. Till now, the best studied bacteriocins are nisin A produced by Lactobacillus lactis and pedocin PA-1 synthesized by Pediococcus acidilactici which have been accredited as a preservative in the food industries by the World Health Organization (WHO). For this study, four different milk and dairy products viz., curd, cheese, yoghurt and butter were collected from the local markets of Karaikal region, Puducherry, India and were used for the isolation of Lactobacillus species using MRS agar. Totally, five morphologically distinct strains were collected and were initially named as MPD 1 to MPD 5. During the screening process of bacteriocin production, the strain MPD 5 showed maximum antimicrobial activity against Vibrio cholerae MTCC 3906 with 900AU/ml. This strain was molecular identified as Lactiplantibacillus plantarum MDP 5 based on 16S rRNA partial sequence method. This 16S rRNA partial sequence was submitted to the NCBI nucleotide GenBank and provided with the accession number, MW301154.1. Further, this strain revealed an enhanced production of bacteriocin using the standardized physicochemical factors such as pH 7, 35°C, 2% fructose and 1% peptone. Furthermore, these optimal conditions revealed more than 2-fold increase in the bacteriocin production. All the above information suggesting the possibilities of bacteriocin for the bioindustrial production using the L. plantarum MDP 5 of this study and its future prospects for the investigation of biocidal activities against many highly infectious pathogens of human and veterinary.

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Association between Healthy Dietary Patterns and Self-Reported Sleep Disturbances in Older Men: The ULSAM Study

Nutrients ◽

10.3390/nu11051029 ◽

2019 ◽

Vol 11 (5) ◽

pp. 1029 ◽

Cited By ~ 3

Author(s):

Lieve van Egmond ◽

Xiao Tan ◽

Per Sjögren ◽

Tommy Cederholm ◽

Christian Benedict

Keyword(s):

Energy Intake ◽

Dietary Patterns ◽

Dairy Products ◽

Sleep Disturbances ◽

Sleep Problems ◽

Older Men ◽

World Health ◽

Food Diary ◽

Milk And Dairy Products ◽

Low Consumption

To date, little is known about how dietary patterns may link to measures of sleep quality in older subjects, who often suffer from sleep problems. Here, we investigated, in an older male population from Sweden (n = 970; aged 71 ± 1 year), whether adherence to the Healthy Diet Indicator (HDI; based on recommendations from the World Health Organization) or the Mediterranean Diet (MD) is linked to sleep disturbances. The diet scores were calculated using a seven-day food diary, and self-reported sleep initiation or maintenance problems were assessed by questionnaires. When adjusted for potential confounders, no associations between dietary scores and sleep parameters were found. In contrast, low consumption of milk and dairy products —one of the dietary features of the MD —was associated with better subjective sleep initiation. This association was, however, not found in men with adequate reports of daily energy intake (~54% of the cohort). To summarize, our findings do not suggest that older men can mitigate perceived difficulties to fall and stay asleep by adhering to either the HDI or MD. Whether low consumption of milk and dairy products can facilitate sleep initiation must be confirmed in future studies by utilizing objective measures of sleep such as polysomnography. Finally, when investigating associations between dietary patterns and sleep, particular attention should be paid to the potential confounder of inadequate reporting of energy intake.

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Levels of Polychlorinated Biphenyls in Foods from Catalonia, Spain: Estimated Dietary Intake

Journal of Food Protection ◽

10.4315/0362-028x-66.3.479 ◽

2003 ◽

Vol 66 (3) ◽

pp. 479-484 ◽

Cited By ~ 66

Author(s):

JUAN M. LLOBET ◽

ANA BOCIO ◽

JOSE L. DOMINGO ◽

ANGEL TEIXIDÓ ◽

CONRAD CASAS ◽

...

Keyword(s):

High Resolution ◽

Dietary Intake ◽

Dairy Products ◽

World Health ◽

Large Contribution ◽

Pcb Congeners ◽

Wet Weight ◽

Toxicity Equivalent ◽

Fish And Shellfish ◽

Milk And Dairy Products

From June to August 2000, food samples were randomly acquired in seven cities in Catalonia, Spain. Polychlorinated biphenyl (PCB) concentrations were determined for 108 samples of vegetables, fruits, pulses, cereals, fish and shellfish, meats and meat products, eggs, milk and dairy products, and oils and fats. Levels of 11 PCB congeners (IUPAC 28, 52, 77, 101, 105, 118, 126, 138, 153, 169, and 180) were determined by high-resolution gas chromatography–high-resolution mass spectrometry. For toxic equivalent (TEQ) calculations, World Health Organization (WHO) toxicity equivalent factors (WHO-TEFs) were used. The highest levels of most congeners were found in fish and shellfish (11,864.18 ng/kg [wet weight]), and the next highest levels, which were substantially lower, were found in milk and dairy products (674.50 ng/kg [wet weight]). For the general population of Catalonia, the total dietary intake of PCBs was found to be 150.13 pg WHO-TEQ/day. The largest contribution to this intake came from fish and shellfish (82.87 pg WHO-TEQ/day) and dairy products (29.38 pg WHO-TEQ per day). A relatively large contribution was also noted for cereals (11.36 pg WHO-TEQ/day). Among the PCB congeners determined in this study, PCB 126 showed the largest contribution to total TEQ intake (50.56%). The data obtained in this study should be useful in risk assessment with regard to human PCB exposure through food in Catalonia.

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Biotype characterization of Staphylococcus aureus from milk and dairy products of private production in the western regions of Ukraine

Regulatory Mechanisms in Biosystems ◽

10.15421/021760 ◽

2017 ◽

Vol 8 (3) ◽

pp. 384-388 ◽

Cited By ~ 1

Author(s):

M. D. Kukhtyn ◽

Y. V. Horyuk ◽

V. V. Horyuk ◽

T. Y. Yaroshenko ◽

O. I. Vichko ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Dairy Products ◽

Meca Gene ◽

Raw Milk ◽

Test System ◽

World Health ◽

Cottage Cheese ◽

Methicillin Resistant ◽

Domestic Production ◽

Milk And Dairy Products

Prevention of foodborne diseases is a priority for the world health system. In the process of manufacturing milk and dairy products, the most important factor endangering their safety is seeding with a conditionally pathogenic and pathogenic microflora. Salmonella, Escherichia coli, Listeria and other microorganisms that reproduce in dairy products without changing their organoleptic properties are a particular danger. Staphylococcus aureus is an opportunistic, conditionally pathogenic microorganism that often contaminates raw milk and dairy products. The aim of the research presented in this article was to determine the dissemination of S. aureus in milk and milk products of household production in the western regions of Ukraine, to identify the biotypes of S. aureus, production of enterotoxins and the presence of methicillin-resistant strains. S. aureus was isolated on BD Baird-Parker Agar. The biotypes of S. aureus were determined according to Meer. The determination of MRSA was carried out on the chromogenic Agar chromID MRSA ("Biomerioux", Russia). The mecA gene was determined using the LightCycler MRSA Advanced Test with LightCycler 2.0 primer (Roche Molecular Biochemicals, Germany). To determine staphylococcal enterotoxins, the test system RIDASCREENSET A, B, C, D, E (R-Biopharm AG, Darmstadt, Germany) was used. We isolated saprophyte staphylococci from milk of raw and dairy products in western regions of Ukraine in 82.7–97.4% of samples. S. aureus is much more rarely isolated from these dairy products, so it was isolated from sour cream at 62.8 ± 0.9%, from milk at 35.5 ± 1.3% and cottage cheese at 23.0 ± 1.6%. Of the most well0known biotypes of S. aureus present in milk of raw and dairy products of domestic production, two ecological types were distinguished: human and cattle. In this case S. aureus var. hominis was isolated more often than in S. aureus var. bovis. This gives grounds to believe that the main source of contamination with milk staphylococci of raw and dairy products of domestic production is people. Enterotoxin type A, which causes foodborne toxemia, was produced by S. aureus in 40.0 ± 0.5% of cases. Consequently, home-produced dairy products can spread staphylococcal toxicity caused by S. aureus var. hominis. It was found that 17.8 ± 0.6% of S. aureus var. hominis were resistant to methicillin, which is 1.8 times greater than that of S. aureus var. bovis. This gives grounds to consider that there is a risk of MRSA infection to consumers of home-produced dairy products. All methicillin-resistant staphylococci studied produced enterotoxins.

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PESTICIDE RESIDUES IN COW MILK AND DAIRY PRODUCTS FROM THE MAJOR MILK PRODUCING AREA OF SRI LANKA

AGROFOR ◽

10.7251/agreng1903083j ◽

2019 ◽

Vol 4 (3) ◽

Author(s):

Jagath JAYASINGHE ◽

Samudra PATHIRANA ◽

Dhammi DILHANI ◽

Senevirathne NAVARATHNA ◽

Manoj SINHAPURA ◽

...

Keyword(s):

Sri Lanka ◽

Pesticide Residues ◽

Dairy Products ◽

Fermented Milk ◽

World Health ◽

Cow Milk ◽

Pasteurized Milk ◽

Fresh Milk ◽

Sterilized Milk ◽

Milk And Dairy Products

Nuwara Eliya district is the leading fresh milk producing area in Sri Lanka. In the district, pesticides are widely applied for intensive cultivation of vegetables which leads to contamination of water and material used to feed cows. Contamination and health risk hazards of organophosphorus pesticide residues in milk and dairy products originated in the district were studied. Identification and quantification of eleven commonly used pesticides in 50 milk samples and 12 dairy product samples were performed using standard analytical methods and GC-MS technique. Results revealed that fresh milk contained residues of Prothiofos (0.0568±0.037 mg/kg), Diazinon (0.0378±0.009 mg/kg), Chlorpyrifos (0.0264±0.004 mg/kg), Profenofos (0.196±0.099 mg/kg), Fipronil (0.1906±0.188 mg/kg), Phenthoate (0.1012±0.110mgkg), Dimethoate (0.1196±0.201 mg/kg) and Tebuconazole (0.062±0.069 mg/kg) at higher levels than the recommended maximum residue levels (MRLs) of the World Health Organization. Sterilized milk (0.0115±0.000 mg/kg) and fermented milk (0.022±0.004 mg/kg) contained higher levels of Profenofos than the MRLs. Higher levels of Fipronil than MRLs were observed in pasteurized milk (0.086±0 mg/kg) and fermented milk (0.014±0.000 mg/kg) samples. Phenthoate at higher levels than MRL was reported in pasteurized milk (0.3645±0.402 mg/kg), sterilized milk (0.1405±0.197 mg/kg) and milk powder (0.0055±0.000 mg/kg). Moreover, Dimethoate content in fermented milk (0.087±0.012 mg/kg) was higher than the MRL. Routine monitoring of the above pollutants in food items including fresh milk and value added milk products is essential to prevent, control and reduce the pollution and to minimize the health risks to consumers.

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