scholarly journals The Effect of Three-Month Atorvastatin and α-Calcidol Administration on Some Bone Tissue Morphometric Parameters

2021 ◽  
Vol 27 (2) ◽  
pp. 65-74
Author(s):  
S. S. Asachuk ◽  
O. S. Yakovleva ◽  
A. F. Martsinkevich ◽  
Е. A. Karpenkа
Keyword(s):  
Bone Tissue ◽  
Cholesterol Metabolism ◽  
Growth Parameters ◽  
Active Form ◽  
Male Rats ◽  
Common Disease ◽  
Osteoporosis Prevention ◽  
Ether Anesthesia ◽  
Combined Use ◽  
Growth Zones

Background.Osteoporosis is the fourth most common disease after cardiovascular diseases, cancer, and diabetes. All these diseases  have  common  pathogenetic  mechanisms  associated  with  impaired  cholesterol  metabolism.  In  recent  decades, the  use  of  the  key  enzyme  of  cholesterol  synthesis  inhibitors —  statins,  which  can  stimulate  osteogenesis,  has  become widespread. However, statins affect the production of the vitamin D active form by reducing the production of testosterone and thus reducing the activity of 1α-hydroxylase. The combined use of statins and α-calcidol (α-C) for the osteoporosis prevention  seems  promising. The  aim  of  the  study was  to  evaluate  the  effect  of  long-term  atorvastatin  (ATV)  and α-C administration on morphometric growth parameters and bone vascularization in the experiment.Materials and Methods. The experiment was conducted for three months on 120 laboratory male rats, which were injected daily intragastrically with ATV and α-C. After 90 days of the experiment, the animals were decapitated under ether anesthesia. For the study, the right femur and jawbone were taken from the animals. The rat bone sections were impregnated with silver, decalcified, and the histosections were stained according to van Gieson. The distribution of the studied features was evaluated according to the Shapiro-Wilk test. The differences were considered statistically significant at p<0.05.Results.It was found that ATV, both separately and together with α-C, increased the size of the newly formed bone in the endoostal and periosteal zones of the femur by 64.8; 40.4 and 15.8; 29.1%, respectively. The combined use of ATV and α-C had a positive effect on the growth of blood vessels in the femur (+23.4%). ATV increased the size of the newly formed bone from the periodontal and vestibular surfaces of the lower jaw by 18.3 and 29.5%, respectively. α-C potentiated the effect of ATV on the size of the newly formed bone tissue in the periodontal and vestibular growth zones of the mandibular bone by 10.1 and 15.0%, respectively. As for the number of vessels in the jawbone tissue, thanks to ATV, it increased by 17.2%, α-C had no effect.Conclusion. ATV increases the thickness of the newly formed bone layer in the growth areas of the femur and jawbone and increases the number of vessels in the jawbone. α-calcidol increases the number of vessels in the bone tissue of the femur and potentiates the effect of ATV on the jawbone growth zones. The combined use of ATV and α-C shows that they positively complement each other.

Relation of Efficacy of Combined Use of Ibandronic Acid and Alfacalcidol with Bone Tissue Remodeling in Women with Postmenopausal Form of Osteoporosis

2011 ◽  
Vol 18 (4) ◽  
pp. 45-49
Author(s):  
Svetlana Semenovna Rodionova ◽  
A A Elovoy-Vronskiy ◽  
S S Rodionova ◽  
A A Elovoy-Vronskiy
Keyword(s):  
Bone Tissue ◽  
Active Form ◽  
Tissue Remodeling ◽  
High Efficacy ◽  
Ibandronic Acid ◽  
Bone Metabolism Markers ◽  
Combined Use ◽  
Vitamin D 3 ◽  
Bone Tissue Remodeling ◽  
Mineral Bone Density

Study of 51 female patients showed high efficacy of alfacalcidol (Alfa D3 - Teva) and ibandronic acid (Bonviva) combination for the treatment of postmenopausal form of systemic osteoporosis. Dependence of mineral bone density (MBD) on the primary intensity of bone tissue remodeling was observed. Assumption on the necessity of bone metabolism markers assessment at ibandronate use was confirmed. The fact that use of active form of vitamin D 3 eliminates such side effects of bisphosphonates as secondary hyperparathyroidism and hypocalcemia has been proved.

THE INFLUENCE OF SORPTION-DETOXIFYING MEANS ON CUMULATION OF 90SR IN BONE TISSUE OF WHITE RATES WITH COMBINED INTAKE OF 137CS, CD AND PB

2018 ◽  
Vol 1 (3) ◽  
pp. 129-133
Author(s):  
L. L. Zakharova ◽  
◽  
G. A. Zhorov ◽  
V. N. Obryvin ◽  
◽  
...  
Keyword(s):  
Bone Tissue ◽  
Chemical Nature ◽  
Mechanisms Of Action ◽  
The Body ◽  
Biologically Active ◽  
Laboratory Studies ◽  
Animal Body ◽  
White Rats ◽  
Combined Use ◽  
Active Substances

The article presents the results of laboratory studies of the effect of sorption-detoxifying means on the accumulation of 90Sr in the body of white rats. The efficiency of a number of selective and polyfunctional sorbents, detoxicants and other biologically active substances and the developed on their basis sorption-detoxifying complexes as means of efferent therapy and detoxification of the animal body at the combined intake of xenobiotics of radiation and chemical nature was evaluated. It was found that 90Sr cumulation in bones (at the level of 53,3–60,8%) was reduced to the greatest extent with the combined use of substances of different mechanisms of action and origin. The effectiveness of separate use of detoxifying drugs did not exceed 29,1%.

scholarly journals On the local damaging effect of biogenic degradation products of the BAK-1000 silicate matrix after implantation (experimental morphological study)

2018 ◽  
pp. 44-49
Author(s):  
А.Ф. Фидаров ◽  
А.С. Григорьян ◽  
Т.Д. Колокольцова ◽  
А.А. Орлов ◽  
Е.Е. Устинова ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Morphological Study ◽  
Degradation Products ◽  
Healing Process ◽  
Male Rats ◽  
Tissue Integrity ◽  
And Function ◽  
Tissue Trauma ◽  
Tissue Restoration ◽  
Late Stages

Актуальность. Травматизм костных тканей остается одной из серьезных проблем современности. Восстановление структуры и функциональности костной ткани больных с посттравматическими поражениями подчеркивает высокую значимость и актуальность проведения исследований по поиску новых методов и материалов для восстановления целостности и оптимизации процессов заживления костной ткани. Целью исследования было экспериментально-морфологическое изучение эффективности применения остеокондуктивного апатитосиликатного композита БАК-1000 в качестве имплантационного материала для восстановления обширного дефекта костной ткани. Методика. Исследования проведены на крысах линии CD, самцах массой 600 ± 10 г (n = 12). После остеотомии с удалением фрагмента кости размером 0,3 х 0,5 см дефект заполняли стерильным имплантационным материалом БАК-1000 в виде цилиндрических блоков того же размера. Контроль восстановления костной ткани проводили гистологическим методом. Результаты показали, что БАК-1000 обладает выраженным местным побочным эффектом, приводящим к возникновению очагов патологического процесса на поздних сроках заживления. Заключение. Данные экспериментально-морфологического исследования подтверждают необходимость проведения более тщательных исследований предлагаемых для использования в клинической практике материалов или композитов на поздних сроках после применения. Background. Bone tissue trauma remains one of serious current challenges. Restoration of the bone tissue structure and function in posttraumatic lesions emphasizes a high relevance of searching for new methods and materials to restore the bone tissue integrity and optimize the healing process. The aim of this experimental morphological study was to evaluate the effectiveness of an osteoconductive apatite-silicate composite BAK-1000 as an implant material for restoration of large defects in bone tissue. Methods. The study was performed on male rats (CD strain) weighing 600 ± 10 g (n = 12). After osteotomy with removal of a 0.3 х 0.5 cm bone fragment, the defect was filled with sterile BAK-1000 implantation material in the form of cylindrical blocks of the same size. The bone tissue restoration process was monitored histologically. The results showed that BAC-1000 had a pronounced local side effect, leading to the emergence of pathological foci at late stages of healing. Conclusion. This experimental morphological study confirmed the need to study more thoroughly the materials or composites proposed for use in clinical practice at late terms following application.

EFFECT OF ERUCIC ACID ON INCORPORATION OF ACETATE-1-C14 INTO CHOLESTEROL AND FATTY ACIDS

1959 ◽  
Vol 37 (7) ◽  
pp. 803-810 ◽  
Author(s):  
K. K. Carroll
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Erucic Acid ◽  
Cholesterol Metabolism ◽  
Young Male ◽  
Male Rats ◽  
Acetate Incorporation ◽  
Acid Incorporation ◽  
Liver Slices ◽  
Fatty Acid Incorporation

Young male rats were fed synthetic diets containing either no fat or various individual fatty acids for 3 to 4 weeks. They were then killed and the incorporation of acetate-1-C14 into cholesterol and fatty acids was measured in liver slices and in scrapings of intestinal mucosa. Acetate incorporation into cholesterol by liver slices was much greater in animals fed erucic acid than in those fed no fat, palmitic, stearic, oleic, or linoleic acids. A marked differential was not observed in fatty acid incorporation but values tended to be higher on the fat-free and erucic acid diets. Erucic acid did not stimulate acetate incorporation into cholesterol by mucosa and in general mucosa seemed to be less sensitive to changes in diet. The results are discussed in relation to previously observed effects of erucic acid on cholesterol metabolism.

Effect of Resistance Training with Palm Pollen and Testosterone on Runx2 Protein and Gene Expression Levels in Bone Tissue of Adult Male Rats

2020 ◽  
Vol 24 (3) ◽  
Author(s):  
Nazila Payandeh ◽  
Maghsoud Peeri ◽  
Mohammad Ali Azarbayjani ◽  
Seyed Ali Hosseini
Keyword(s):  
Gene Expression ◽  
Resistance Training ◽  
Bone Tissue ◽  
Healthy Lifestyle ◽  
Male Rats ◽  
Expression Levels ◽  
Protein Levels ◽  
Related Transcription Factor ◽  
And Training ◽  
Gene Expression Levels

Background: A healthy lifestyle, nutrition, and exercise can improve bone mass via several mechanisms. Objectives: This study assessed the effects of four weeks of palm pollen consumption along with resistance training on protein and gene expression levels of Runt-related transcription factor 2 (Runx2) in bone tissue of rats. Methods: Thirty-six rats were selected and assigned into six groups, including (1) training + testosterone, (2) training + palm pollen, (3) testosterone, (4) palm pollen, (5) training and (6) sham. Then, 100 mg/kg of palm pollen was prescribed five days per week. Resistance training was performed five sessions per week, and 2 mg/kg of testosterone propionate was prescribed peritoneally. Gene expression and protein levels of Runx2 were measured via the real-time PCR and Western blot methods. Results: Training had a significant effect on the increase in Runx2 protein levels (P ≤ 0.05). Training + testosterone, training + palm pollen, testosterone, and palm pollen had significant effects on gene expression and protein levels of Runx2 (P ≤ 0.05). Training + testosterone and training + palm pollen had more favorable effects on the increase of gene expression and protein levels of Runx2 than had testosterone, palm pollen, and training (P ≤ 0.05). Conclusions: Although training, palm pollen, and testosterone alone could increase the Runx2 protein levels in the bone tissue of rats, training with palm pollen and training with testosterone appeared to have more favorable effects on the increase of gene expression and protein levels of Runx2 than either alone.

Assessment of efficiency of complex treatment of soft tissue wounds by means of oxytocin and titanium aquacomplex glycerosolvate in experiment

10.12737/4115 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 1-4
Author(s):  
Глухов ◽  
A. Glukhov ◽  
Мохова ◽  
Olesya Mokhova ◽  
Микулич ◽  
...  
Keyword(s):  
Clinical Study ◽  
Soft Tissue ◽  
Granulation Tissue ◽  
Inflammatory Cells ◽  
Male Rats ◽  
Laboratory Animals ◽  
Complex Treatment ◽  
High Efficacy ◽  
Combined Use ◽  
White Mongrel

Purpose of the study is to assess the efficiency use of oxytocin and titanium aquacomplex glycerosol-vate in the treatment of soft tissue wounds. The study was carried out on 72 white mongrel male rats weighing 210 ± 10 g with simulated aseptic wounds. The sampling was carried out for 1, 3, 5 and 7 days from the beginning of the experiment. To assess the course of wound process in the clinical study, planimetric and histological methods were applied. The comparison of obtained results has allowed to reveal the high efficacy of combined use of oxytocin and glycerosolvate titanium. On the basis of clinical and planimetric methods of investigation it was established that reduction of the area of the wound is significantly faster in animals treated with the use of oxytocin and glycerosolvate titanium. This treatment has a stimulating effect on the activity of connective tissue - accelerates the appearance and maturation of granulation tissue, occur earlier replacement of a number of inflammatory cells into cells, which provide proliferation; earlier appearance of collagen fibers and the prevalence among them horizontally. The use of oxytocin and titanium aquacomplex glycerosolvate in laboratory animals with aseptic wounds influences positively on the dynamic of wounded process, allows to accelerate the appearance of granulation tissue and epithelialization.

Effects of High-Phosphorus and/or Low-Calcium Diets on Bone Tissue in Trained Male Rats

2011 ◽  
Vol 43 (1) ◽  
pp. 54-63 ◽  
Author(s):  
LAURENT BÉGOT ◽  
JEAN-MARC COLLOMBET ◽  
SYLVIE RENAULT ◽  
XAVIER BUTIGIEG ◽  
CATHERINE ANDRÉ ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Male Rats ◽  
High Phosphorus ◽  
Low Calcium

scholarly journals Gastric estradiol-17β (E2) and liver ERα correlate with serum E2 in the cholestatic male rat

2013 ◽  
Vol 219 (1) ◽  
pp. 39-49 ◽  
Author(s):  
Hiroto Kobayashi ◽  
Saori Yoshida ◽  
Ying-Jie Sun ◽  
Nobuyuki Shirasawa ◽  
Akira Naito
Keyword(s):  
Portal Vein ◽  
Cholesterol Metabolism ◽  
Male Rats ◽  
Male Rat ◽  
Rt Pcr ◽  
Steroid Dependent ◽  
Duct Ligation ◽  
Female Specific ◽  
Male Specific ◽  
Estradiol 17Β

Cholestasis is associated with changes in hepatic cholesterol metabolism and serum estrogen levels. Ueyama and colleagues reported that the gastric estradiol-17β (E2) level in the portal vein is several times higher than that in the artery. This study aimed to clarify the relationships between gastric E2, hepatic estrogen receptor (ER) α and cholesterol metabolism in cholestatic male rats induced by bile duct ligation (BDL). After BDL, serum E2 levels in the portal vein and artery were measured by ELISA. The gene expression of gastric estrogen-synthesizing enzymes and various hepatic enzymes for cholesterol metabolism were measured by real-time RT-PCR, and gastric aromatase and hepatic ERα proteins were determined by immunohistochemistry and western blotting. Portal E2 levels increased by 4.9, 5.0, and 3.6 times that of controls at 2 days after BDL (BDL2d), BDL4d, and BDL7d respectively. The change in arterial E2 levels was positively correlated with that in the portal vein. Under these conditions, the expression of hepatic Ers1 (ERα) mRNA and protein was significantly reduced in a negative correlation with serum E2 levels in the portal vein after BDL. The expression of hepatic male-specific cytochrome P450 (CYP) genes Cyp2c55 and Cyp3a2 decreased and female-specific Cyp2c12 increased after BDL. It is postulated that the increase in gastric E2 levels, which occurs after BDL, results in the reduction of hepatic ERα, the elevation of arterial E2 level and leads to cholesterol metabolism becoming sex steroid dependent.

scholarly journals Level of Osteotropic Elements in the Jaw Bones of Experimental Animals in Terms of the Impact of Xenobiotics

2019 ◽  
Vol 26 (2) ◽  
Author(s):  
Mariana Kaminska ◽  
Mykola Rozhko ◽  
Hanna Ersteniuk
Keyword(s):  
Heavy Metals ◽  
Bone Tissue ◽  
Male Rats ◽  
Control Group ◽  
Metabolic Processes ◽  
Jaw Bone ◽  
Intact Group ◽  
Jaw Bones ◽  
Zinc Levels ◽  
The Impact

Pollution of the environment with heavy metals, pesticides, nitrates leads to an unceasing deterioration of the population’s health, in particular, the dental one. Excessive intake of salts of heavy metals and nitrites is accompanied by changes in calcium-phosphorus metabolism rates, indicating a disorder of metabolic processes in bone tissue. This necessitates the study of the level of osteotropic elements of the jaw bone tissue. Materials and methods of research. In the experiment on 80 white outbred male rats, the effects of cadmium chloride, sodium nitrite and their combined action on the content of osteotropic elements in the jaw bone were studied. The control group consisted of 10 intact animals. Results. It was determined that in the dynamics (1st, 14th, 28th days) of both cadmium-nitrite, nitrite and cadmium intoxication there was observed the accumulation of cadmium and decrease of calcium, copper, zinc levels in bone tissue of animal jaws compared to intact group. Conclusions.The most significant changes in the level of osteotropic elements in the jaw bones are found in the combined effect of xenobiotics, which is important for understanding of the metabolic processes in the oral cavity.

scholarly journals The regulatory role of the RANKL/RANK/OPG signaling pathway in the mechanisms of tooth eruption in patients with impacted teeth

2020 ◽  
Author(s):  
Ludmila Brodetska ◽  
Larysa Natrus ◽  
Olha Lisakovska ◽  
Olexandr Kaniura ◽  
Liudmyla Iakovenko ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Bone Tissue ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Caspase 3 ◽  
Active Form ◽  
Tooth Eruption ◽  
Control Group ◽  
Impacted Teeth ◽  
Tooth Impaction

Abstract Background: Tooth impaction is a common problem in orthodontic practice and in some cases accompanied by pain and pathological changes of surrounding teeth. Understanding the cellular and molecular mechanisms underlying tooth impaction allows finding the most effective orthodontic treatment for patients with impacted teeth (IT). RANK (receptor activator of NF-κB) / RANKL (RANK ligand) / OPG (osteoprotegerin) signaling pathway controls bone resorption and may be involved in the regulation of tooth eruption. The study aimed to evaluate bone remodeling based on the assessment of the RANKL/RANK/OPG status in patients with IT. Methods: Bone samples from 18 patients (mean age 25.27±3.34) were divided into 3 groups: 1 – bone tissue of healthy persons (control group); 2 – bone tissue, that was taken near the healthy tooth in patients with tooth impaction; 3 – bone tissue, that was collected near the IT. Levels of RANKL, RANK, OPG, osteocalcin (OC), NF-κB p65 subunit, NFATc1, and caspase-3 were determined by western blotting. The difference between groups was assessed using ANOVA followed by Tukey’s post-hoc test. P-value ≤ 0.05 was considered statistically significant.Results: We established a 1.73-fold elevation of RANK level in the IT area vs. control, indicating the recruitment of preosteoclasts. An increase in RANKL, OPG, and OC content was demonstrated (1.46-, 1.48-, and 1.42-fold respectively), reflecting the high activity of osteoblasts near the IT. Despite the activation of the RANKL/RANK/OPG system in the impaction area, NF-κB and NFATc1 levels did not change compared vs. control, indicating a blocked/delayed process of osteoclastogenesis. We found a decrease in the content of procaspase-3 (1.28-fold), while the level of its active form p17 increased by 2.26 folds near the healthy tooth in patients with IT compared with control. In the area of ​​IT, we observed an increase in procaspase-3 and p17 levels (1.32 and 1.78 folds). This reflects impairments of caspase-3 activation and accumulation of its inactive form in the IT area that may contribute to the tooth eruption failure.Conclusions: Tooth impaction may be associated with the disturbances in the caspase-3 cascade activation and the imbalance in the RANKL/RANK/OPG system, and as a result, blocked bone resorption.

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