Cold Atmospheric Plasma Induces HMGB1 Expression in Cancer Cells

AbstractPlasma oncology is a relatively new field of research. Recent developments have indicated that cold atmospheric plasma (CAP) technology is an interesting new therapeutic approach to cancer treatment. In this study, p53 wildtype (LoVo) and human p53 mutated (HT29 and SW480) colorectal cancer cells were treated with the miniFlatPlaSter – a device particularly developed for the treatment of tumor cells – that uses the Surface Micro Discharge (SMD) technology for plasma production in air. The present study analyzed the effects of plasma on colorectal cancer cellsin vitroand on normal colon tissueex vivo. Plasma treatment had strong effects on colon cancer cells, such as inhibition of cell proliferation, induction of cell death and modulation of p21 expression. In contrast, CAP treatment of murine colon tissueex vivofor up to 2 min did not show any toxic effect on normal colon cells compared to H2O2positive control. In summary, these results suggest that the miniFlatPlaSter plasma device is able to kill colorectal cancer cells independent of their p53 mutation status. Thus, this device presents a promising new approach in colon cancer therapy.

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Selective ablation of ocular cancer cells and cancer stem cells with Cold atmospheric plasma

Journal of Clinical & Experimental Ophthalmology ◽

10.4172/2155-9570.s1.021 ◽

2015 ◽

Vol 06 (03) ◽

Author(s):

Arpitha Parthasarathy

Keyword(s):

Stem Cells ◽

Cancer Stem Cells ◽

Cancer Cells ◽

Atmospheric Plasma ◽

Cold Atmospheric Plasma ◽

Selective Ablation ◽

Ocular Cancer

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Cold Atmospheric Plasma for Selectively Ablating Metastatic Breast Cancer Cells

PLoS ONE ◽

10.1371/journal.pone.0073741 ◽

2013 ◽

Vol 8 (9) ◽

pp. e73741 ◽

Cited By ~ 116

Author(s):

Mian Wang ◽

Benjamin Holmes ◽

Xiaoqian Cheng ◽

Wei Zhu ◽

Michael Keidar ◽

...

Keyword(s):

Breast Cancer ◽

Metastatic Breast Cancer ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Metastatic Breast ◽

Atmospheric Plasma ◽

Cold Atmospheric Plasma

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The Specific Vulnerabilities of Cancer Cells to the Cold Atmospheric Plasma-Stimulated Solutions

Scientific Reports ◽

10.1038/s41598-017-04770-x ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 40

Author(s):

Dayun Yan ◽

Haitao Cui ◽

Wei Zhu ◽

Niki Nourmohammadi ◽

Julian Milberg ◽

...

Keyword(s):

Cancer Cells ◽

Atmospheric Plasma ◽

Cold Atmospheric Plasma

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Cold Atmospheric Plasma Selectively Induces G0/G1 Cell Cycle Arrest and Apoptosis in AR-Independent Prostate Cancer Cells

10.21203/rs.3.rs-49986/v1 ◽

2020 ◽

Author(s):

Meng Ning ◽

Zhifa Zhang ◽

Lihui Yu ◽

Peiyu Han ◽

xiaofeng Dai

Keyword(s):

Prostate Cancer ◽

Cell Cycle ◽

Androgen Receptor ◽

Cancer Cells ◽

Atmospheric Plasma ◽

Prostate Cancer Cells ◽

Cold Atmospheric Plasma ◽

Prostate Cancers ◽

And Migration ◽

Physical Plasma

Abstract BackgroundAndrogen receptor-independent prostate cancers do not respond to androgen blockage therapies and suffer from high recurrence rate. We aim to contribute to the establishment of novel therapeutic approaches against such malignancies.Methods We examined whether and how cold atmospheric plasma delivers selectivity against AR-independent prostate cancers using human normal epithelial prostatic cells PNT1A and AR-negative DU145 prostate cancer cells.ResultsWe show that cold atmospheric plasma could selectively halt cell proliferation and migration in androgen receptor-independent cells as a result of induced cell apoptosis and G0/G1 stage cell cycle arrest, and such outcomes were achieved through modulations on the MAPK and NF-kB pathways in response to physical plasma induced intracellular redox level. ConclusionOur study reports cold atmospheric plasma induced reduction on the proliferation and migration of androgen receptor-independent prostate cancer cells that offers novel therapeutic insights on the treatment of such cancers, and provides the first evidence on physical plasma induced cell cycle G0/G1 stage arrest that warrants the exploration on the synergistic use of cold atmospheric plasma and drugs such as chemotherapies in eradicating such cancer cells.

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Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer

BioMed Research International ◽

10.1155/2021/1969863 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Zahra Yazdani ◽

Pooyan Mehrabanjoubani ◽

Alireza Rafiei ◽

Pourya Biparva ◽

Mostafa Kardan

Keyword(s):

Gene Expression ◽

Combination Therapy ◽

Cancer Cells ◽

In Silico ◽

Cancer Cell Line ◽

Combined Effect ◽

Atmospheric Plasma ◽

Concomitant Treatment ◽

Cold Atmospheric Plasma ◽

Melanoma Cancer

Curcumin (CUR) has interesting properties to cure cancer. Cold atmospheric plasma (CAP) is also an emerging biomedical technique that has great potential for cancer treatment. Therefore, the combined effect of CAP and CUR on inducing cytotoxicity and apoptosis of melanoma cancer cells might be promising. Here, we investigated the combined effects of CAP and CUR on cytotoxicity and apoptosis in B16-F10 melanoma cancer cells compared to L929 normal cells using MTT method, acridine orange/ethidium bromide fluorescence microscopic assay, and Annexin V/PI flow cytometry. In addition, the activation of apoptosis pathways was evaluated using BCL2, BAX, and Caspase-3 (CASP3) gene expression and ratio of BAX to BCL2 (BAX/BCL2). Finally, in silico study was performed to suggest the molecular mechanism of this combination therapy on melanoma cancer. Results showed that although combination therapy with CUR and CAP has cytotoxic and apoptotic effects on cancer cells, it did not improve apoptosis rate in melanoma B16-F10 cancer cells compared to monotherapy with CAP or CUR. In addition, evaluation of gene expression in cancer cell line confirmed that CUR and CAP concomitant treatment did not enhance the expression of apoptotic genes. In silico analysis of docked model suggested that CUR blocks aquaporin- (AQP-) 1 channel and prevents penetration of CAP-induced ROS into the cells. In conclusion, combination therapy with CAP and CUR does not improve the anticancer effect of each alone.

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Mismatch of frequencies of ac voltage and streamers propagation in cold atmospheric plasma jet for typical regimes of cancer cell treatment

Journal of Physics Conference Series ◽

10.1088/1742-6596/2100/1/012020 ◽

2021 ◽

Vol 2100 (1) ◽

pp. 012020

Author(s):

I Schweigert ◽

A Alexandrov ◽

D Zakrevsky ◽

E Milakhina ◽

E Patrakova ◽

...

Keyword(s):

Cancer Cells ◽

Cancer Cell ◽

Plasma Jet ◽

Plasma Source ◽

Atmospheric Plasma ◽

Modes Of Operation ◽

Cold Atmospheric Plasma ◽

Mode Of Operation ◽

Voltage Frequency

Abstract Cold atmospheric plasma (CAP) jet generated by the plasma source at 2-6 kV ac voltages with frequencies of 10-50 kHz demonstrate the different modes of operation. Depending on the voltage frequency and amplitude, some streamers in the plasma jet are short and decay before they approach the treated surface. In this case, the effect on the viability of cancer cells when exposed to CAP jet strongly depends on the mode of operation of the discharge or, in other words, on how many times the streamers hit the bio-target during the treatment. The effect on different modes on cancer cells A549 viability is reported.

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