Mismatch of frequencies of ac voltage and streamers propagation in cold atmospheric plasma jet for typical regimes of cancer cell treatment

Abstract Cold atmospheric plasma (CAP) jet generated by the plasma source at 2-6 kV ac voltages with frequencies of 10-50 kHz demonstrate the different modes of operation. Depending on the voltage frequency and amplitude, some streamers in the plasma jet are short and decay before they approach the treated surface. In this case, the effect on the viability of cancer cells when exposed to CAP jet strongly depends on the mode of operation of the discharge or, in other words, on how many times the streamers hit the bio-target during the treatment. The effect on different modes on cancer cells A549 viability is reported.

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Investigation of a cold atmospheric plasma jet generation in single and multichannel planar devices

Journal of Physics Conference Series ◽

10.1088/1742-6596/2064/1/012127 ◽

2021 ◽

Vol 2064 (1) ◽

pp. 012127

Author(s):

P P Gugin ◽

D E Zakrevsky ◽

E V Milakhina

Keyword(s):

Applied Voltage ◽

Atmospheric Pressure ◽

Plasma Jet ◽

Plasma Source ◽

Atmospheric Plasma ◽

Planar Geometry ◽

Collector Current ◽

Cold Atmospheric Plasma

Abstract A plasma source of atmospheric pressure with a planar geometry of the device and an adjustable number of planar discharge channels has been developed. The dependence of the recorded collector current on the amplitude of the applied voltage is investigated. It was found that the current in each of the channels consists of a set of independent current channels whose propagation does not depend on each other.

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Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/4205640 ◽

2020 ◽

Vol 2020 ◽

pp. 1-17

Author(s):

Ara Jo ◽

Hea Min Joh ◽

Tae Hun Chung ◽

Jin Woong Chung

Keyword(s):

Flow Cytometry ◽

Cancer Cells ◽

Cancer Cell ◽

Atmospheric Pressure ◽

A549 Cells ◽

Plasma Jet ◽

Reactive Species ◽

Atmospheric Plasma ◽

Anticancer Effects ◽

Indirect Treatment

Cold atmospheric plasma (CAP) has been reported to have strong anticancer effects in vitro and in vivo. CAP has been known to induce apoptosis in most cancer cells by treatment to cells using direct and indirect treatment methods. There are many reports of apoptosis pathways induced by CAP, but for indirect treatment, there is still a lack of fundamental research on how CAP can cause apoptosis in cancer cells. In this study, we applied an indirect treatment method to determine how CAP can induce cancer cell death. First, plasma-activated medium (PAM) was produced by a 2.45 GHz microwave-excited atmospheric pressure plasma jet (ME-APPJ). Next, the amounts of various reactive species in the PAM were estimated using colorimetric methods. The concentration of NO2– and H2O2 in PAM cultured with cancer cells was measured, and intracellular reactive oxidative stress (ROS) changes were observed using flow cytometry. When PAM was incubated with A549 lung cancer cells, there was little change in NO2– concentration, but the concentration of H2O2 gradually decreased after 30 min. While the intracellular ROS of A549 cells was rapidly increased at 2 hours, there was no significant change in that of PAM-treated normal cells. Furthermore, PAM had a significant cytotoxic effect on A549 cells but had little effect on normal cell viability. In addition, using flow cytometry, we confirmed that apoptosis of A549 cells occurred following flow cytometry and western blot analysis. These results suggest that among various reactive species produced by PAM, hydrogen peroxide plays a key role in inducing cancer cell apoptosis.

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Tiny Cold Atmospheric Plasma Jet for Biomedical Applications

Processes ◽

10.3390/pr9020249 ◽

2021 ◽

Vol 9 (2) ◽

pp. 249

Author(s):

Zhitong Chen ◽

Richard Obenchain ◽

Richard E. Wirz

Keyword(s):

Biomedical Applications ◽

Plasma Jet ◽

Discharge Voltage ◽

Plasma Jets ◽

Atmospheric Plasma ◽

Physical Parameters ◽

Cold Atmospheric Plasma ◽

Water Metal ◽

Plasma Probe ◽

Jet Nozzle

Conventional plasma jets for biomedical applications tend to have several drawbacks, such as high voltages, high gas delivery, large plasma probe volume, and the formation of discharge within the organ. Therefore, it is challenging to employ these jets inside a living organism’s body. Thus, we developed a single-electrode tiny plasma jet and evaluated its use for clinical biomedical applications. We investigated the effect of voltage input and flow rate on the jet length and studied the physical parameters of the plasma jet, including discharge voltage, average gas and subject temperature, and optical emissions via spectroscopy (OES). The interactions between the tiny plasma jet and five subjects (de-ionized (DI) water, metal, cardboard, pork belly, and pork muscle) were studied at distances of 10 mm and 15 mm from the jet nozzle. The results showed that the tiny plasma jet caused no damage or burning of tissues, and the ROS/RNS (reactive oxygen/nitrogen species) intensity increased when the distance was lowered from 15 mm to 10 mm. These initial observations establish the tiny plasma jet device as a potentially useful tool in clinical biomedical applications.

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Evaluation of photodynamic effect of Indocyanine green (ICG) on the colon and glioblastoma cancer cell lines pretreated by cold atmospheric plasma

Photodiagnosis and Photodynamic Therapy ◽

10.1016/j.pdpdt.2021.102408 ◽

2021 ◽

pp. 102408

Author(s):

Elham Shayesteh Ara ◽

Atefeh Vejdani Noghreiyan ◽

Ameneh Sazgarnia

Keyword(s):

Indocyanine Green ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Atmospheric Plasma ◽

Photodynamic Effect ◽

Cold Atmospheric Plasma

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Human Mononuclear Cell Survival and Proliferation is Modulated by Cold Atmospheric Plasma Jet

Plasma Processes and Polymers ◽

10.1002/ppap.201300008 ◽

2013 ◽

Vol 10 (8) ◽

pp. 706-713 ◽

Cited By ~ 44

Author(s):

Sander Bekeschus ◽

Kai Masur ◽

Julia Kolata ◽

Kristian Wende ◽

Anke Schmidt ◽

...

Keyword(s):

Cell Survival ◽

Mononuclear Cell ◽

Plasma Jet ◽

Atmospheric Plasma ◽

Cold Atmospheric Plasma ◽

Human Mononuclear Cell ◽

Cell Survival And Proliferation

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Cold atmospheric plasma treatment inhibits growth in colorectal cancer cells

Biological Chemistry ◽

10.1515/hsz-2018-0193 ◽

2018 ◽

Vol 400 (1) ◽

pp. 111-122 ◽

Cited By ~ 10

Author(s):

Christin Schneider ◽

Stephanie Arndt ◽

Julia L. Zimmermann ◽

Yangfang Li ◽

Sigrid Karrer ◽

...

Keyword(s):

Colorectal Cancer ◽

Colon Cancer ◽

Plasma Treatment ◽

Cancer Cells ◽

Ex Vivo ◽

Atmospheric Plasma ◽

Normal Colon ◽

Colon Tissue ◽

Cold Atmospheric Plasma ◽

Colorectal Cancer Cells

AbstractPlasma oncology is a relatively new field of research. Recent developments have indicated that cold atmospheric plasma (CAP) technology is an interesting new therapeutic approach to cancer treatment. In this study, p53 wildtype (LoVo) and human p53 mutated (HT29 and SW480) colorectal cancer cells were treated with the miniFlatPlaSter – a device particularly developed for the treatment of tumor cells – that uses the Surface Micro Discharge (SMD) technology for plasma production in air. The present study analyzed the effects of plasma on colorectal cancer cellsin vitroand on normal colon tissueex vivo. Plasma treatment had strong effects on colon cancer cells, such as inhibition of cell proliferation, induction of cell death and modulation of p21 expression. In contrast, CAP treatment of murine colon tissueex vivofor up to 2 min did not show any toxic effect on normal colon cells compared to H2O2positive control. In summary, these results suggest that the miniFlatPlaSter plasma device is able to kill colorectal cancer cells independent of their p53 mutation status. Thus, this device presents a promising new approach in colon cancer therapy.

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