THE INFLUENCE OF GILT AGE ON THE ENDOCRINE AND OVULATORY RESPONSE TO DIFFERENT DOSES OF ESTRADIOL BENZOATE

1988 ◽  
Vol 68 (3) ◽  
pp. 979-982 ◽  
Author(s):  
R. N. KIRKWOOD ◽  
F. X. AHERNE
Keyword(s):  
Body Weight ◽  
Luteinizing Hormone ◽  
Key Words ◽  
Jugular Vein ◽  
Estradiol Benzoate ◽  
Blood Samples ◽  
Estrous Cyclicity ◽  
The Mean ◽  
Different Doses ◽  
Phasic Release

Fifty-five prepubertal Yorkshire × Landrace gilts were randomly assigned to one of six treatments involving the injection (i.m.) of estradiol benzoate (EB) at either 10 or 20 μg kg−1 body weight at gilt ages of 130, 150 or 170 d. Blood samples were taken by jugular vein puncture at 0, 24, and 36 h, and then at 6-h intervals until 84 h, after injection and assayed for plasma luteinizing hormone (LH) concentrations. The mean magnitude of the EB-induced LH peak was 6.1 ± 0.2 ng mL−1, and was not affected by treatment. Fifty-six percent of gilts ovulated in response to EB injection, and 34% had a subsequent ovulation. The initial ovulation rate was unaffected by EB dose, but was higher (P < 0.05) in 170 d (9.9 ± 1.0) compared to younger gilts (4.2 ± 1.1 and 5.5 ± 1.1 for 130-dand 150-d gilts, respectively). Subsequent ovulation rates were unaffected by treatment. It is concluded that EB treatment will usually induce a phasic release of LH from prepubertal gilts, but this release is not necessarily accompanied by normal estrous cyclicity. Key words: Estradiol, gilts, ovulation

Association of Retained Fetal Membranes with Haematological Profile in Crossbred Cows

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
K. Rokde ◽  
S. Kumar ◽  
A. Bhardwaz ◽  
S. S. Mahour ◽  
S. P. Nema ◽  
...  
Keyword(s):  
Eosinophil Count ◽  
Jugular Vein ◽  
Dairy Farm ◽  
Fetal Membranes ◽  
Monocyte Count ◽  
Blood Samples ◽  
Mean Values ◽  
Crossbred Cows ◽  
Haematological Profile ◽  
The Mean

This study was carried out on clinical cases of retained fetal membranes in crossbred cows presented at College Clinics and College dairy farm and from Villages in and around Mhow. The blood samples were collected from jugular vein just before 12 hr. postpartum and on 7th day postpartum. Haematological profile revealed that the mean values of haemoglobin, neutrophil and monocyte count after 12 hrs and 7th day postpartum were significantly lower and lymphocyte count was significantly higher in RFM cows (n=18) than normally calved cows (n=6). The differences in mean TLC, eosinophil and basophil counts were non-significant at 12 hrs postpartum, however on 7th day postpartum the TLC and eosinophil count were significantly higher and basophil count was non-significantly different in RFM cows than the normally calved cows.

Pattern of plasma progesterone, oestradiol-17β, luteinizing hormone and androgen in non-pregnant buffalo (Bubalus bubalis)

1982 ◽  
Vol 100 (2) ◽  
pp. 279-284 ◽  
Author(s):  
R. C. Arora ◽  
R. S. Pandey
Keyword(s):  
Luteinizing Hormone ◽  
Peak Level ◽  
Systemic Circulation ◽  
Bubalus Bubalis ◽  
High Concentration ◽  
Plasma Progesterone ◽  
Blood Samples ◽  
Androgen Level ◽  
The Mean ◽  
Low Levels

Abstract. Domestic buffaloes were used to characterize the pattern of progesterone, oestradiol-17β, LH and androgen in the systemic circulation following infertile insemination. Concentrations of hormones were measured by RIA in blood samples collected daily or at alternate days following insemination. The concentration of progesterone was lowest on the day of insemination, and increased significantly to a peak level of 4.00 ± 0.60 ng/ml by day 13 post insemination. After day 17, it declined significantly (P < 0.01) to reach low levels by day 21. The concentration of oestradiol-17β was high at the time of insemination and declined significantly (P < 0.01) by day 2 after insemination. It was maintained around the basal level till day 18 with minor peaks in between this period. It again rose significantly (P < 0.01) at subsequent oestrus. The mean level of LH was highest at the time of insemination, and declined significantly (P < 0.01) by day 1 post insemination. It did not vary appreciably till the animal returned to oestrus. The oestrous value of LH and progesterone were negatively correlated (r = −0.77). The androgen level was observed to be high at insemination in 3 out of 5 animals, but the overall pattern of this steroid was inconsistent during the period studied. A high concentration of androgen was recorded in all the animals from day 2–5 before the onset of oestrus.

THE INFLUENCE OF GROWTH HORMONE TREATMENT ON THYROID FUNCTION IN SWINE

1990 ◽  
Vol 70 (3) ◽  
pp. 991-995 ◽  
Author(s):  
R. N. KIRKWOOD ◽  
P. A. THACKER ◽  
B. LAARVELD
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Key Words ◽  
Growth Hormone Treatment ◽  
Sampling Period ◽  
Hormone Treatment ◽  
Serum Concentrations ◽  
Significant Treatment ◽  
Blood Samples ◽  
To Receive

Twelve castrated male pigs of Yorkshire and Landrace breeding were selected at 95.9 ± 1.6 kg body weight (BW) and allocated equally to receive daily injections of either porcine growth hormone (pGH) at 90 μg kg−1 BW or vehicle buffer for four consecutive days. Following the last pGH injection, the pigs were infused via indwelling vena caval cannulae with thyrotropin-releasing hormone (TRH) at 0.5 μg kg−1 BW. Blood samples were obtained at 10 and 0 min before TRH and thereafter at 10-min intervals for 90 min. Serum concentrations of thyroxine and thyrotropin were lower (P < 0.06 and P < 0.1, respectively) but those of triiodothyronine higher (P < 0.01) in pGH-treated pigs throughout the sampling period. There were no significant treatment-by-time interactions indicating that the thyroid response to TRH was not influenced by pGH treatment. Key words: Swine, thyroid, growth hormone, somatotropin

Effect of insulin on the concentration of glucose and hormones in serum and milk yield of postpartum ewes

1991 ◽  
Vol 71 (4) ◽  
pp. 1253-1256 ◽  
Author(s):  
T. C. Pope ◽  
D. M. Hallford
Keyword(s):  
Body Weight ◽  
Key Words ◽  
Milk Yield ◽  
Glucose Concentration ◽  
Serum Insulin ◽  
Insulin Injection ◽  
Serum Glucose ◽  
Exogenous Insulin ◽  
Blood Samples ◽  
Ovarian Cyclicity

Eighteen mature Debouillet ewes with single lambs received either 0, 0.25 or 0.50 IU Lente insulin per kilogram body weight per day on days 11 through 20 postpartum. Hourly blood samples were obtained from 1 h before to 6 h after treatment on days 11 and 20. Serum insulin concentration was increased by 1 h after insulin injection on day 11 and was accompanied by a decrease in serum glucose concentration. By 6 h after treatment on days 11 and 20, serum glucose and insulin in ewes receiving 0.25 IU insulin had returned to control levels. Exogenous insulin did not induce ovarian cyclicity. Key words: Sheep, insulin, postpartum, anestrus, prolactin, somatotropin

scholarly journals Effects of Withania coagulans extract and morphine on spermatogenesis in rats

2021 ◽  
Vol 18 (4) ◽  
pp. 817-822
Author(s):  
Ali Noori ◽  
Leila Amjad ◽  
Fereshteh Yazdani
Keyword(s):  
Body Weight ◽  
Luteinizing Hormone ◽  
Methanol Extract ◽  
Histopathological Examination ◽  
Follicle Stimulating Hormone ◽  
Testicular Tissue ◽  
Reproductive Parameters ◽  
Blood Samples ◽  
Significant Difference ◽  
Sistan And Baluchestan

Purpose: To investigate the comparative effects of Withania coagolans extract and morphine on spermatogenesis in rats Methods: W. coagolans was collected from Sistan and Baluchestan, Iran and 50 and 100 mg/kg body weight doses of methanol extract and 5, 10 and 15 mg/kg body weight doses of morphine were administered parenterally to the rats which were divided into groups. Blood samples were collected and the levels of luteinizing hormone (LH), follicle stimulating hormone (FSH), and testosterone were assayed. The testicular tissue was isolated for histopathological examination. Results: No significant changes were observed in levels of LH, FSH and testosterone in treated groups (p < 0.05). However, there was significant difference between the treated groups for extract plus morphine groups, in terms of the number of spermatogonium, spermatocytes and spermatide variation. Moreover, the results indicate tissue disorders in all groups relative to control. The extract caused more disturbances in spermatogenesis compared to morphine, and appears to improve parameters related to spermatogenesis. Conclusion: The results show that the higher dose of Withania coagolans extract (100 mg/kg) exerts varying effects on reproductive parameters. Moreover, the lower dose of Withania coagolans extract (50 mg/kg) enhanced spermatogenesis while also protecting against the damaging effects of morphine.

The influence of nutrition on reproduction in the ewe 2. Effects of undernutrition on pituitary responsiveness to luteinizing hormone-releasing hormone stimulation

1981 ◽  
Vol 32 (3) ◽  
pp. 257-260 ◽  
Author(s):  
W. Haresign
Keyword(s):  
Body Weight ◽  
Luteinizing Hormone ◽  
Hormone Release ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Hormone Injection ◽  
Good Body Condition ◽  
Chronic Undernutrition ◽  
The Mean ◽  
Maintenance Ration

ABSTRACTTwo groups of ewes in good body condition at the start of the breeding season were given either sufficient feed to maintain their body weight or 500 g/kg of a maintenance ration such that they showed a continuous fall in body weight. On three separate occasions after introducing the nutritional regimens, all ewes were challenged on day 12 of the oestrous cycle with 150 μg luteinizing hormone-releasing hormone. Prolonged chronic undernutrition resulted in a significant increase in luteinizing hormone release in response to luteinizing hormone-releasing hormone injection (P < 0·05), whereas the mean pre-injection luteinizing hormone concentrations were significantly reduced by undernutrition (P < 0·01).

Changes in ovarian follicular dynamics and luteinizing hormone profiles following different progestagen treatments in cattle

1994 ◽  
Vol 74 (2) ◽  
pp. 273-279 ◽  
Author(s):  
C. Taylor ◽  
M. Manikkam ◽  
R. Rajamahendran
Keyword(s):  
Luteinizing Hormone ◽  
Key Words ◽  
Pulse Frequency ◽  
Implant Removal ◽  
Dominant Follicle ◽  
Blood Samples ◽  
Treatment Groups ◽  
Lactating Cows ◽  
Intravaginal Device ◽  
Follicular Dynamics

Twelve cyclic non-lactating cows were allocated at random to three treatment groups (IN, NP and 2N). Cows in the 1N group (n = 4) received an ear implant containing Norgestomet at pro-estrus (day 0 = day of Norgestomet-implant insertion). Cows in the NP group (n = 4) received a progesterone-releasing intravaginal device 3 d after a Norgestomet implant at pro-estrus, and cows in the 2N group (n = 4) received a second Norgestomet implant 3 d after the first implant at pro-estrus. Treatments were terminated on day 9. Ultrasonic examination of the ovaries was carried out before, during and after treatment to monitor follicular dynamics. Serial blood samples were collected every 15 min for 8 h from all cows on days 3 and 6 after initiation of treatments to characterize the luteinizing hormone (LH) profiles. The dominant follicle was maintained for 9 d in all IN cows, and upon implant removal all cows ovulated. In both NP and 2N cows, the dominant follicle regressed and a new cohort of small follicles appeared before the end of the treatment. The dominant follicle from this pool ovulated after implant removal in all NP cows. In the 2N group, only one cow ovulated after implant removal; the dominant follicles in the remainder became cystic and did not ovulate. Decreases in pulse frequency, amplitude and mean plasma LH were observed in the NP cows, compared with IN cows (P < 0.05), but no change in LH-pulse frequency was observed in the 2N cows. This study shows clearly that maintenance and regression of the dominant follicle are associated with changes in progesterone and LH environment. Key words: Progestin, dominant follicle, ultrasound, regression, luteinizing hormone, cattle

scholarly journals Anti-hyperglycemic Effect of Stephania Glabra Tubers in Alloxan Induced Diabetic Mice

1970 ◽  
Vol 11 (1) ◽  
pp. 17-19 ◽  
Author(s):  
Deepak Kumar Semwal ◽  
Usha Rawat ◽  
Ruchi Badoni ◽  
Ravindra Semwal ◽  
Randhir Singh
Keyword(s):  
Body Weight ◽  
Oral Administration ◽  
Key Words ◽  
Ethanolic Extract ◽  
Hypoglycemic Activity ◽  
Diabetic Mice ◽  
Antihyperglycemic Activity ◽  
Hypoglycemic Agent ◽  
Different Doses ◽  
Hyperglycemic Effect

Different doses of ethanolic extract of Stephania glabra tuber were evaluated for antihyperglycemic activity in alloxan induced diabetic mice. The oral administration of 100, 200 and 500 mg/kg body weight showed significant hypoglycemic activity. Glibenclamide (oral hypoglycemic agent, 25 mg/kg, p.o.) has been used as standard. Key words: Stephania glabra, Menispermaceae, hydroxypalmatine, alloxan, glibenclamide, hypoglycemic activity DOI:10.3329/jom.v11i1.4262 J Medicine 2010: 11: 17-19

THE INFLUENCE OF EXOGENOUS GROWTH HORMONE ON ENDOCRINE STATUS AND THE OVULATORY RESPONSE TO PMSG IN PREPUBERTAL GILTS

1990 ◽  
Vol 70 (1) ◽  
pp. 309-312 ◽  
Author(s):  
R. N. KIRKWOOD ◽  
P. A. THACKER ◽  
R. S. KORCHINSKI ◽  
B. LAARVELD
Keyword(s):  
Growth Hormone ◽  
Key Words ◽  
Jugular Vein ◽  
Serum Concentrations ◽  
Blood Samples ◽  
Exogenous Growth ◽  
Endocrine Status ◽  
The Difference ◽  
To Receive ◽  
Surge Height

Twenty-three prepubertal Yorkshire × Landrace gilts were allocated to receive daily injections of either porcine growth hormone (pGH) or vehicle for 6 consecutive days. At the time of first pGH or buffer injection, all gilts received an injection of 500 IU PMSG. Blood samples were taken by jugular vein puncture at 0, 24 and 48 h then every 12 h until 96 h then every 8 h until 144 h. Samples were assayed for triiodothyronine (T3), insulin, glucose and LH. Treatment with pGH elevated (P < 0.01) serum concentrations of T3, insulin and glucose, and was associated with a reduced (P < 0.05) LH surge height. Fewer pGH-treated than vehicle-treated gilts ovulated (4/12 vs. 7/11), but the difference was not significant. Key words: Gilts, growth hormone, gonadotrophins, ovulation

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