Efficacy of different fungicides and biopesticides for the management of lentil wilt (Fusarium oxysporum f. sp. lentis)

In Vitro and In Vivo studies on the efficacy of fungicides and biopesticides. Among the fungicides, in Carbedazim to the most effective as they have inhibited the mycelia growth completely of the test fungus, and Benomyl, Topsin - M, Ridomil,Vitavax were found the next best in inhibiting the mycelial growth of the pathogen up to 92.11% to 83.46% respectively. Sadabahar was least effective plant extracts which causes 42 mm of radial growth and inhibited the growth of the only 19.23%. In Vivo condition the maximum seed germination (95.50% and 95.33%), minimum wilt incidence (5.16% and 3.65%) and highest grain yield (10.50 q/ha and 10.35 q/ha) was found seed treatment with Carbendazim (0.2%). Among the test plant extracts Tulsi was lested effective, which show the minimum seed germination (80.00% and 77.50%), maximum wilt incidence (15.70% and 14.10%), and lowest grain yield (3.92 q/ha and 4.17 q/ha).

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Prunella vulgaris L. Potentiates Answer to the Emergence of Dreaded Antibiotic Resistance

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.15.3.35 ◽

2021 ◽

Author(s):

Jitendra K. Patel ◽

Chetan Kumar Joshi ◽

Mukesh Kumar Sharma

Keyword(s):

Petroleum Ether ◽

Plant Extracts ◽

Herbal Extract ◽

In Vivo Studies ◽

Human Beings ◽

Prunella Vulgaris ◽

Reduced Pressure ◽

E Coli

Medicinal herbs that are in use for centuries to treat infections and other illnesses. Prunella vulgaris L. is traditionally used for its therapeutic attributes for the alleviation of various infectious diseases. The objective of this study on Prunella vulgaris was to reveal relevant pharmaceutical information to understand its beneficial medicinal uses for human beings. The methanolic and petroleum ether extracts after removal of the solvent under reduced pressure from the Prunella vulgaris plants were prepared and these extracts were analyzed in vitro for their activity against B. subtilis, E. coli, S. aureus and S. typhi (with ATCC numbers 6051, 25922, 23235 and 14028 respectively). Likewise, in vivo studies were conducted using the E. coli-induced peritonitis in laboratory rat models where the rats were given allopathic antibiotic ofloxacin and the results were compared with those rats who received plant extracts under controlled conditions. The results were analyzed for the efficacy of the plant extracts was compared with ofloxacin; the methanol extracts exhibited equally if not better results in clearing the pathogen from the system of animals. The petroleum ether extracts exhibited the least antimicrobial activity in comparison to those extracted in methanol isolates. In conclusion, the herbal extract demonstrated significant antibacterial activity both under in-vitro and in-vivo studies which is a major outcome of the study. During this study, all standards and norms were followed as per government animal authority.

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Antischistosomal Activity of Origanum majorana, Ziziphus spina-christi, and Salvia fruticosa Plant Extracts on Hamster Infected with Schistosoma haematobium

BioMed Research International ◽

10.1155/2021/5545331 ◽

2021 ◽

Vol 2021 ◽

pp. 1-15

Author(s):

Yousef Abdal Jalil Fadladdin

Keyword(s):

Plant Extracts ◽

Schistosoma Haematobium ◽

Early Stage ◽

World Health ◽

In Vivo Studies ◽

Salvia Fruticosa ◽

Medical Plant ◽

Origanum Majorana

World Health Organization (WHO) has approved only one treatment for schistosomiasis, praziquantel (PZQ), but some poor efficacy was noticed in patients during the early stage of infection. Therefore, researchers have intensified their efforts to research new alternative medicines to treat schistosomiasis. In the present study, in vitro as well as in vivo studies have been accomplished to evaluate the effect of Origanum majorana, Ziziphus spina-christi, and Salvia fruticosa extracts in a different concentration 500, 250, 125, 62.5, and 31.25 μg/ml on golden hamster infected by Egyptian strains of schistosome (Schistosoma haematobium). In vitro, the adult worms and schistosomula of S. haematobium were investigated in RPMI-1640 medium for 48 hrs. The results showed that the concentration 500, 250, and 125 μg/ml of Origanum majorana, and Ziziphus spina-christi caused dead of 100% of Egyptian Schistosoma strains of adult worm and schistosomula of S. haematobium within 6 to 12 hrs of incubation. On the other hand, the extract of Salvia fruticosa at concentrations 500, 250, and 125 μg/ml showed death 100% parasites after 12 to 24 hrs of incubation. Inclusion, Origanum majorana, and Ziziphus spina-christi showed effectiveness against Egyptian Schistosoma strains (S. haematobium), a slight decrease in Salvia fruticosa was observed. Therefore, these medical plant extracts may be used as a safe and effective treatment for schistosomiasis.

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Antimicrobial and antioxidant activities of edible coatings enriched with natural plant extracts: In vitro and in vivo studies

Postharvest Biology and Technology ◽

10.1016/j.postharvbio.2008.02.013 ◽

2008 ◽

Vol 49 (2) ◽

pp. 294-300 ◽

Cited By ~ 204

Author(s):

Alejandra G. Ponce ◽

Sara I. Roura ◽

Carlos E. del Valle ◽

María R. Moreira

Keyword(s):

Plant Extracts ◽

Antioxidant Activities ◽

In Vivo Studies ◽

Edible Coatings ◽

Natural Plant

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The Modulatory Influence of Plant-Derived Compounds on Human Keratinocyte Function

International Journal of Molecular Sciences ◽

10.3390/ijms222212488 ◽

2021 ◽

Vol 22 (22) ◽

pp. 12488

Author(s):

Anna Merecz-Sadowska ◽

Przemysław Sitarek ◽

Karolina Zajdel ◽

Ewa Kucharska ◽

Tomasz Kowalczyk ◽

...

Keyword(s):

Wound Healing ◽

Plant Extracts ◽

Reactive Oxygen ◽

Secondary Compounds ◽

Protective Role ◽

In Vivo Studies ◽

Cytokines And Chemokines ◽

Oxygen Scavenging

The plant kingdom is a rich source of secondary metabolites with numerous properties, including the potential to modify keratinocyte biology. Keratinocytes are important epithelial cells that play a protective role against various chemical, physical and biological stimuli, and participate in reactive oxygen scavenging and inflammation and wound healing processes. The epidermal cell response may be modulated by phytochemicals via changes in signal transduction pathways. Plant extracts and single secondary compounds can possess a high antioxidant capacity and may suppress reactive oxygen species release, inhibit pro-apoptotic proteins and apoptosis and activate antioxidant enzymes in keratinocytes. Moreover, selected plant extracts and single compounds also exhibit anti-inflammatory properties and exposure may result in limited production of adhesion molecules, pro-inflammatory cytokines and chemokines in keratinocytes. In addition, plant extracts and single compounds may promote keratinocyte motility and proliferation via the regulation of growth factor production and enhance wound healing. While such plant compounds may modulate keratinocyte functions, further in vitro and in vivo studies are needed on their mechanisms of action, and more specific toxicity and clinical studies are needed to ensure their effectiveness and safety for use on human skin.

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Phytochemicals in Helicobacter pylori Infections: What Are We Doing Now?

International Journal of Molecular Sciences ◽

10.3390/ijms19082361 ◽

2018 ◽

Vol 19 (8) ◽

pp. 2361 ◽

Cited By ~ 35

Author(s):

Bahare Salehi ◽

Farukh Sharopov ◽

Miquel Martorell ◽

Jovana Rajkovic ◽

Adedayo Ademiluyi ◽

...

Keyword(s):

Helicobacter Pylori ◽

Plant Extracts ◽

Bioactive Molecules ◽

Herbal Remedies ◽

In Vivo Studies ◽

Modes Of Action ◽

Urease Inhibitory ◽

H Pylori

In this critical review, plant sources used as effective antibacterial agents against Helicobacter pylori infections are carefully described. The main intrinsic bioactive molecules, responsible for the observed effects are also underlined and their corresponding modes of action specifically highlighted. In addition to traditional uses as herbal remedies, in vitro and in vivo studies focusing on plant extracts and isolated bioactive compounds with anti-H. pylori activity are also critically discussed. Lastly, special attention was also given to plant extracts with urease inhibitory effects, with emphasis on involved modes of action.

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In vitro studies of inhibitory activity of plant extracts and cow urine on mycelial growth of stem rot, Sclerotimoryzae of rice

Journal of Applied and Natural Science ◽

10.31018/jans.v9i1.1142 ◽

2017 ◽

Vol 9 (1) ◽

pp. 24-28 ◽

Cited By ~ 1

Author(s):

Nishant Prakash ◽

A.P. Sinha

Keyword(s):

Antifungal Activity ◽

Plant Extracts ◽

Mycelial Growth ◽

Cent Concentration ◽

Maximum Inhibition ◽

Mycelia Growth ◽

Cow Urine ◽

Chemical Pesticides

Soil borne phytopathogen Sclerotiumoryzae significantly affect rice production. To reduce load of chemical pesticides, antifungal activity of plant extracts and cow urine against mycelial growth of S.oryzaewere tested using poisoned food technique under in vitro condition. Plant extracts of 2.5%, 5.0%, 7.5% and 10% concentration was prepared from Allium cepa, Azadirachtaindica, A. sativum, Ricinuscommunisand Syzygiumcumini. Inhibition of mycelial growth of S.oryzaewas recorded only in case of A. sativum and A. cepa while Azadirachtaindica, Ricinuscommunisand Syzygiumcumini did not show any inhibition of mycelial growth as compared to control. A.sativum plant extracts showed maximum inhibition of mycelia growth of 68.88% at concentration 10% followed by 32.96%, 22.96% and 18.88% at concentration 7.5%, 5.0% and 2.5% resepectively. 22.60%, 19.62%, 17.77% and 8.88% inhibition of mycelial growth as compared to control was recorded at 10%, 7.5%, 5.0% and 2.5% concentration of plant extracts of A.cepa. All the concentration of cow urine inhibited the mycelial growth of S. oryzae. Cow urine at the concentration 5, 7.5 and 10.0 per cent resulted in 100 per cent inhibition of mycelia growth of test pathogen as compared to control. Maximum inhibition of 98.14 per cent was observed at 2.5 per cent concentration followed by 1.25 per cent (63.7%) concentration. This study showed that A.sativum and A.cepa and cow urine possess antifungal activity under in vitro condition. It can also be tested for antifungal activity under in vivo condition.

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Antiulcer Agents: From Plant Extracts to Phytochemicals in Healing Promotion

Molecules ◽

10.3390/molecules23071751 ◽

2018 ◽

Vol 23 (7) ◽

pp. 1751 ◽

Cited By ~ 46

Author(s):

Mehdi Sharifi-Rad ◽

Patrick Fokou ◽

Farukh Sharopov ◽

Miquel Martorell ◽

Adedayo Ademiluyi ◽

...

Keyword(s):

Helicobacter Pylori ◽

Plant Extracts ◽

Safety Profile ◽

Mechanisms Of Action ◽

Herbal Remedies ◽

In Vivo Studies ◽

Antiulcer Agents ◽

Plant Sources

In this narrative review, we have comprehensively reviewed the plant sources used as antiulcer agents. From traditional uses as herbal remedies, we have moved on to preclinical evidence, critically discussing the in vitro and in vivo studies focusing on plant extracts and even isolated phytochemicals with antiulcerogenic potential. A particular emphasis was also paid to Helicobacter pylori activity, with emphasis on involved mechanisms of action. Lastly, the issue of safety profile of these plant products has also been addressed.

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A systematic review on anti-malarial drug discovery and antiplasmodial potential of green synthesis mediated metal nanoparticles: overview, challenges and future perspectives

Malaria Journal ◽

10.1186/s12936-019-2974-9 ◽

2019 ◽

Vol 18 (1) ◽

Cited By ~ 4

Author(s):

Loick P. Kojom Foko ◽

Francois Eya’ane Meva ◽

Carole E. Eboumbou Moukoko ◽

Agnes A. Ntoumba ◽

Marie I. Ngaha Njila ◽

...

Keyword(s):

Systematic Review ◽

Green Synthesis ◽

Metal Nanoparticles ◽

Plant Extracts ◽

Artemisinin Resistance ◽

In Vivo Studies ◽

Recent Emergence ◽

Positive Controls

Abstract Background The recent emergence in Southeast Asia of artemisinin resistance poses major threats to malaria control and elimination globally. Green nanotechnologies can constitute interesting tools for discovering anti-malarial medicines. This systematic review focused on the green synthesis of metal nanoparticles as potential source of new antiplasmodial drugs. Methods Seven electronic database were used following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Results A total of 17 papers were included in the systematic review. 82.4% of the studies used plant leaves to produce nanoparticles (NPs) while three studies used microorganisms, including bacteria and fungi. Silver was the main metal precursor for the synthesis of NPs. The majority of studies obtained nanoparticles spherical in shape, with sizes ranging between 4 and 65 nm, and reported no or little cytotoxic effect of the NPs. Results based on 50% inhibitory concentration (IC50) varied between studies but, in general, could be divided into three NP categories; (i) those more effective than positive controls, (ii) those more effective than corresponding plant extracts and, (iii) those less effective than the positive controls or plant extracts. Conclusions This study highlights the high antiplasmodial potential of green-synthesized metal nanoparticles thereby underscoring the possibility to find and develop new anti-malarial drugs based on green synthesis approaches. However, the review also highlights the need for extensive in vitro and in vivo studies to confirm their safety in humans and the elucidation of the mechanism of action. Graphical abstract

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