scholarly journals Antibody response after hepatitis B vaccine boost mapped with peptide-phage display

Bionatura ◽  
2019 ◽  
Vol 02 (Bionatura Conference Serie) ◽  
Author(s):  
Lisbeth Ramírez Caballero ◽  
Nicolas Delaroque ◽  
Michael Szardenings
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Phage Display ◽  
In Silico ◽  
Hepatitis B Surface Antigen ◽  
In Silico Analysis ◽  
Hepatitis B Vaccination ◽  
Serum Samples ◽  
B Virus ◽  
Peptide Phage Display

Recombinant hepatitis B virus vaccines confer protection by eliciting specific antibodies against the hepatitis B surface antigen (HBsAg), known as anti-HBs. However, the performance of rapid anti-HBs diagnostic tests generates concerns regarding consistency. Novel indicators of protection might be developed by monitoring changes in targeted HBsAg-epitope profile after vaccination. In this work, we test the feasibility of our peptide-phage display platform in identifying B-cell epitopes targeted at different time-points after hepatitis B vaccination. We combined this platform with a unique approach for in silico analysis of enriched sequences. Serum samples collected from one single patient who had two boosting immunizations against hepatitis B virus were used in two-rounds of selection experiments. Five epitope candidates from HBsAg were identified in silico; most of them were previously reported in the literature. Our results suggest that the number of recognized HBsAg epitopes is related to the decrease of anti-HBs over time.

scholarly journals In Silico Analysis of Epitope-Based Vaccine Candidates against Hepatitis B Virus Polymerase Protein

Viruses ◽  
2017 ◽  
Vol 9 (5) ◽  
pp. 112 ◽  
Author(s):  
Juzeng Zheng ◽  
Xianfan Lin ◽  
Xiuyan Wang ◽  
Liyu Zheng ◽  
Songsong Lan ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
In Silico ◽  
In Silico Analysis ◽  
Vaccine Candidates ◽  
B Virus ◽  
Silico Analysis

In silico analysis of mutations associated with occult hepatitis B virus (HBV) infection in South Africa

2021 ◽  
Author(s):  
Matthew Olagbenro ◽  
Motswedi Anderson ◽  
Simani Gaseitsiwe ◽  
Eleanor A. Powell ◽  
Maemu P. Gededzha ◽  
...  
Keyword(s):  
South Africa ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
In Silico ◽  
In Silico Analysis ◽  
Hbv Infection ◽  
Occult Hepatitis ◽  
B Virus ◽  
Occult Hepatitis B ◽  
Silico Analysis

scholarly journals Protein-x of hepatitis B virus in interaction with CCAAT/enhancer-binding protein α (C/EBPα) - an in silico analysis approach

2011 ◽  
Vol 8 (1) ◽  
pp. 41 ◽  
Author(s):  
Ashraf Mohamadkhani ◽  
Parisa Shahnazari ◽  
Zarrin Minuchehr ◽  
Armin Madadkar-Sobhani ◽  
Mahmoud Tehrani ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
In Silico ◽  
Binding Protein ◽  
In Silico Analysis ◽  
Analysis Approach ◽  
Enhancer Binding Protein ◽  
B Virus ◽  
Protein X ◽  
Silico Analysis

scholarly journals Seroprevalence of Hepatitis B Virus Infection among the Tribal Population of Attapady, Kerala,India

2021 ◽  
Author(s):  
Irene Jose Manjiyil ◽  
Kavitha Paul Konikkara
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hepatitis B Surface Antigen ◽  
Hbv Infection ◽  
Health Concern ◽  
Core Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tribal Population ◽  
Serum Samples ◽  
B Virus

Introduction: Hepatitis B Virus (HBV) infection remains a significant global health concern that may cause acute or chronic hepatitis. Chronically infected patients are at risk for cirrhosis and hepatocellular carcinoma. The disease causes a problem in the tribal communities. There are lack of studies on the prevalence of HBV among the tribal population. Aim: To assess the seroprevalence of HBV infection among the tribal population of Attapady, Kerala. Materials and Methods: This was a community based cross- sectional study conducted on serum samples collected from 269 subjects among the tribal population of Attapady. Serum samples were tested for quantitative antibody to HBsAg (anti-HBs), Hepatitis B surface antigen (HBsAg) and Hepatitis B envelope antigen (HBeAg) Enzyme Linked Immunosorbent Assay (ELISA). Total hepatitis B core antibody (anti-HBc) and IgM antibody to hepatitis B core antigen (anti HBc IgM), frequencies were obtained using proportion and 95% Confidence Interval CI. Results: The seroprevalence of HBsAg was 10.4%. HBeAg was detected in 7.1% of HBsAg positive patients. 21.2% had protective anti-HBs titer. Anti-HBe was detected in five patients. Anti-HBc total and anti-HBc IgM were positive for 26.7% and 2.6%, respectively. Anti-HBc IgM alone and isolated anti-HBc were detected in 1.5% and 5.9 %, respectively. Anti-HBs and anti-HBc total both became positive in 8.6% cases. Conclusion: HBV infection poses a huge burden on tribal health. All HBsAg positive patients should be tested further to determine the stage of the disease. There is need to explore high HBV prevalence areas with studies on associated risk factors to bring out the ongoing transmission process and focus on preventive measures. HBV vaccination, antenatal screening, and health awareness should be given priority to tackle the burden.

scholarly journals Comparison of Three Luminescent Immunoassays for Hepatitis B Virus Surface Antigen Quantification during the Natural History of Chronic Hepatitis B Virus Infection

2014 ◽  
Vol 21 (11) ◽  
pp. 1521-1527 ◽  
Author(s):  
Xiao-Dong Cheng ◽  
Liu-Wei Song ◽  
Lin-Lin Fang ◽  
Lin Yang ◽  
Yong Wu ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Serum Samples ◽  
Virus Surface ◽  
Chronic Hepatitis B Virus ◽  
B Virus

ABSTRACTHepatitis B surface antigen (HBsAg) quantification has garnered attention because of its high predictive value in determining treatment responses. The HBsAg quantification assays, such as Architect and Elecsys, are commercially available, and more assays are in development. We aimed to compare the results of the Architect and Elecsys assays with those of a new assay, WTultra. The WTultra HBsAg assay is a sandwich chemiluminescent microplate enzyme immunoassay and provides an alternative choice which is more cost-effective and potentially applicable in developing or resource-constrained countries and areas. A total of 411 serum samples were collected from patients during various phases of chronic hepatitis B (CHB) infection. The samples were assessed using the three assays, and the results were compared and analyzed. The results for the Architect, Elecsys, and WTultra assays were well correlated according to the overall results for the samples (correlation coefficients,rArchitect versus WTultra= 0.936,rArchitect versus Elecsys= 0.952, andrWTultra versus Elecsys= 0.981) and the various infection phases (rArchitect versus WTultraranging from 0.67 to 0.975,rArchitect versus Elecsysranging from 0.695 to 0.982, andrWTultra versus Elecsysranging from 0.877 to 0.99). Additionally, consistent results were observed according to genotype (genotype B:rArchitect versus WTultra= 0.976,rArchitect versus Elecsys= 0.978, andrWTultra versus Elecsys= 0.979; genotype C:rArchitect versus WTultra= 0.950,rArchitect versus Elecsys= 0.963, andrWTultra versus Elecsys= 0.981) and hepatitis B virus (HBV) DNA levels (rArchitect= 0.540,rWTultra= 0.553, andrElecsys= 0.580). In conclusion, the Elecsys and WTultra assays were well correlated with the Architect assay, irrespective of the CHB infection phase or genotype. All of these assays are reliable for HBsAg quantification.

scholarly journals Characterization of Unusual Escape Variants of Hepatitis B Virus Isolated from a Hepatitis B Surface Antigen-Negative Subject

1998 ◽  
Vol 72 (9) ◽  
pp. 7692-7696 ◽  
Author(s):  
Stefanie Grethe ◽  
Masyar Monazahian ◽  
Ingo Böhme ◽  
Reiner Thomssen
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Vaccine Development ◽  
Core Protein ◽  
Hepatitis B Surface Antigen ◽  
Surface Proteins ◽  
Serum Samples ◽  
B Virus

ABSTRACT Hepatitis B virus DNA was extracted from serial serum samples of a hepatitis B surface antigen-negative patient with antibodies to the core protein as the only marker of an infection with hepatitis B virus. This patient showed no symptoms of hepatic injury. Sequencing of the amplified viral DNA demonstrated multiple amino acid changes clustering in surface-exposed regions of the surface protein. Synthesis and association of the middle (M) and small (S) surface proteins could be shown in vitro. The variant surface antigens were recognized neither by monoclonal antibodies to the surface antigen nor by the vaccinee’s sera. Consequences for hepatitis B surface antigen testing and vaccine development are discussed.

scholarly journals PREVALENCE OF HEPATITIS B VIRUS AND ASSOCIATED RISK FACTORS IN IRULA TRIBAL POPULATION

2017 ◽  
Vol 10 (8) ◽  
pp. 100 ◽  
Author(s):  
Ramya Dinesh ◽  
Ramalakshmi S
Keyword(s):  
Risk Factors ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Tamil Nadu ◽  
Hepatitis B Surface Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tribal Population ◽  
Serum Samples ◽  
Major Health Problem ◽  
B Virus

 Objective: The study is to analyze the prevalence of infections caused by hepatitis B virus (HBV), and to analyze of risk factors for hepatitis B surface antigen (HBsAg) transmission among the Irula tribes of Tamil Nadu.Methods: Serum samples were collected from 350 participants of Irula tribes from 15 different locations of Tamil Nadu. All serum samples were tested for serological markers of HBV (HBsAg) by 3rd generation enzyme-linked immunosorbent assay kit, and the data were subjected to analyze using SPSS (version 17.0) and Chi-square test to determine the risk factors of HBV among Irula tribes.Results: In the study, HBsAg positivity was observed in a higher percentage in males 10 (8.47%) than females 9 (3.54%) and their all age groups indicate the high prevalence of HBV infection in Irula tribes. Analysis of risk factors showed that jaundice in family (JF), tattooing, series of injection, sexual promiscuity, and surgery with blood transfusion plays a major role in transmission in spread of HBV.Conclusion: Hepatitis B is a major health problem in Irula tribes and needs to design intervention strategies among Irula tribal population.

scholarly journals In Silico Analysis of Hepatitis B Virus Occult Associated Mutations in Botswana Using a Novel Algorithm

Genes ◽  
2018 ◽  
Vol 9 (9) ◽  
pp. 420 ◽  
Author(s):  
Motswedi Anderson ◽  
Wonderful Choga ◽  
Sikhulile Moyo ◽  
Trevor Bell ◽  
Tshepiso Mbangiwa ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Protein Function ◽  
In Silico ◽  
In Silico Analysis ◽  
Occult Hepatitis ◽  
B Virus ◽  
Chronic Hbv ◽  
The Impact

Occult hepatitis B infections (OBI) represent a reservoir of undiagnosed and untreated hepatitis B virus (HBV), hence the need to identify mutations that lead to this phenotype. Functionally characterizing these mutations by in vitro studies is time-consuming and expensive. To bridge this gap, in silico approaches, which predict the effect of amino acid (aa) variants on HBV protein function, are necessary. We developed an algorithm for determining the relevance of OBI-associated mutations using in silico approaches. A 3 kb fragment of subgenotypes A1 and D3 from 24 chronic HBV-infected (CHB) and 24 OBI participants was analyzed. To develop and validate the algorithm, the effects of 68 previously characterized occult-associated mutations were determined using three computational tools: PolyPhen2, SNAP2, and PROVEAN. The percentage of deleterious mutations (with impact on protein function) predicted were 52 (76.5%) by PolyPhen2, 55 (80.9%) by SNAP2, and 65 (95.6%) by PROVEAN. At least two tools correctly predicted 59 (86.8%) mutations as deleterious. To identify OBI-associated mutations exclusive to Botswana, study sequences were compared to CHB sequences from GenBank. Of the 43 OBI-associated mutations identified, 26 (60.5%) were predicted by at least two tools to have an impact on protein function. To our knowledge, this is the first study to use in silico approaches to determine the impact of OBI-associated mutations, thereby identifying potential candidates for functional analysis to facilitate mechanistic studies of the OBI phenotype.

scholarly journals The prevalence of hepatitis B in Sweden; A statistical serosurvey of 3381 Swedish inhabitants

1997 ◽  
Vol 119 (2) ◽  
pp. 221-225 ◽  
Author(s):  
B. CHRISTENSON ◽  
M. BÖTTIGER ◽  
L. GRILLNER
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hepatitis B Surface Antigen ◽  
Hepatitis B Vaccination ◽  
Core Antigen ◽  
Swedish Population ◽  
Age Related ◽  
B Virus ◽  
Hepatitis B Core Antigen ◽  
Hepatitis B Virus Markers

The prevalence of hepatitis B virus markers in the adult Swedish population was investigated according to age, sex, origin and demographic stratum. Sera were collected from 3382 persons in 1990–1. The sera were selected on a statistical basis considered to be representative of the Swedish population from adults aged [les ]18 years. Two of the sera (0·06%) were found to be hepatitis B surface antigen positive. The two hepatitis B carriers were of non-Scandinavian origin as were (8·9%) of those tested. A total of 90 persons had a marker of previous, hepatitis B virus infection, i.e. antibodies against hepatitis B core antigen. Of these, 66 (2·0%) were of Scandinavian origin and 24 (18·1%) from highly endemic areas. The overall hepatitis B virus marker prevalence was 2·7%. The highest age-specific prevalence of hepatitis B markers in those of Scandinavian origin was in those born in 1939 and earlier. In this age-group, women had a significantly higher prevalence (3·6%) than males (1·9%). The lowest prevalence was found in those born in 1970 and later. No significant, age-related differences between younger or older persons, or between men and women, could be found in persons of non-Scandinavian origin. The results showed significant differences in exposure to hepatitis B virus among the indigenous population, compared with those of non-Scandinavian extraction. The results do not support the proposal to include hepatitis B vaccination in the Swedish immunization schedule.

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