An Alternative Process for Butanol Production: Continuous Flash Fermentation

2008 ◽  
Vol 3 (1) ◽  
Author(s):  
Adriano Pinto Mariano ◽  
Dejanira de Franceschi de Angelis ◽  
Francisco Maugeri Filho ◽  
Daniel Ibraim Pires Atala ◽  
Maria Regina Wolf Maciel ◽  
...  
Keyword(s):  
Process Development ◽  
Inhibitory Effect ◽  
Development Stage ◽  
Final Concentration ◽  
Environmental Benefits ◽  
Sugar Solution ◽  
Butanol Production ◽  
Retention System ◽  
Butanol Concentration ◽  
Sugar Conversion

The objective of this work is to introduce and demonstrate the technical feasibility of the continuous flash fermentation for the production of butanol. The evaluation was carried out through mathematical modeling and computer simulation which is a good approach in such a process development stage. The process consists of three interconnected units, as follows: the fermentor, the cell retention system (tangential microfiltration) and the vacuum flash vessel (responsible for the continuous recovery of butanol from the broth). The efficiency of this process was experimentally validated for the ethanol fermentation, whose main results are also shown. With the proposed design the concentration of butanol in the fermentor was lowered from 11.3 to 7.8 g/l, which represented a significant reduction in the inhibitory effect. As a result, the final concentration of butanol was 28.2 g/l for a broth with 140 g/l of glucose. Solvents productivity and yield were, respectively, 11.7 g/l.h and 33.5 % for a sugar conversion of 95.6 %. Positive aspects about the flash fermentation process are the solvents productivity, the use of concentrated sugar solution and the final butanol concentration. The last two features can be responsible for a meaningful reduction in the distillation costs and result in environmental benefits due to lower quantities of wastewater generated by the process.

scholarly journals Efficient Strategy to Alleviate the Inhibitory Effect of Lignin-Derived Compounds for Enhanced Butanol Production

2020 ◽  
Author(s):  
Shrikant A. Survase ◽  
Pranhita Nimbalkar ◽  
German Jurgens ◽  
Tom Granström ◽  
Prakash Chavan ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Butanol Production ◽  
Efficient Strategy

scholarly journals Optimization of Reaction Selectivity Using CFD-Based Compartmental Modeling and Surrogate-Based Optimization

Processes ◽  
2018 ◽  
Vol 7 (1) ◽  
pp. 9 ◽  
Author(s):  
Shu Yang ◽  
San Kiang ◽  
Parham Farzan ◽  
Marianthi Ierapetritou
Keyword(s):  
Process Development ◽  
Reaction Network ◽  
Development Stage ◽  
Competitive Reaction ◽  
Reaction Selectivity ◽  
Unit Operations ◽  
Surrogate Based Optimization ◽  
Process Operation ◽  
Reaction Unit ◽  
Optimization Methodology

Mixing is considered as a critical process parameter (CPP) during process development due to its significant influence on reaction selectivity and process safety. Nevertheless, mixing issues are difficult to identify and solve owing to their complexity and dependence on knowledge of kinetics and hydrodynamics. In this paper, we proposed an optimization methodology using Computational Fluid Dynamics (CFD) based compartmental modelling to improve mixing and reaction selectivity. More importantly, we have demonstrated that through the implementation of surrogate-based optimization, the proposed methodology can be used as a computationally non-intensive way for rapid process development of reaction unit operations. For illustration purpose, reaction selectivity of a process with Bourne competitive reaction network is discussed. Results demonstrate that we can improve reaction selectivity by dynamically controlling rates and locations of feeding in the reactor. The proposed methodology incorporates mechanistic understanding of the reaction kinetics together with an efficient optimization algorithm to determine the optimal process operation and thus can serve as a tool for quality-by-design (QbD) during product development stage.

Coupling Coordination Analysis of Urban Land Intensive Use Benefits Based on TOPSIS Method in Xianning City

2014 ◽  
Vol 1073-1076 ◽  
pp. 1387-1392 ◽  
Author(s):  
Ya Qun Wang ◽  
Cheng Shun Song ◽  
Zheng Xiong
Keyword(s):  
General System ◽  
Theoretical Models ◽  
Urban Land ◽  
Development Stage ◽  
Economic Benefits ◽  
Environmental Benefits ◽  
Intensive Use ◽  
High Coordination ◽  
Coordination Degree

With the theory and method of general system science, the paper, by taking Xianning City as a case study, established coupling coordination theoretical models of socio-economic benefits and eco-environmental benefits of urban land intensive use, then analyzed socio-economic benefits and eco-environment benefits of urban land intensive use, coupling degree and coupling coordinative degree between urban land intensive use socio-economic and eco-environmental by using the statistical data during 1999-2010. The results showed that: (1) The socio-economic benefits of urban land intensive use increased rapidly, however, the eco-environmental benefits of urban land intensive use increased in a fluctuation; (2) The coupling degrees of urban land intensive use socio-economic benefits and eco-environmental benefits was at an antagonism development stage, the coupling degrees decreased slowly; (3) The coupling coordination degree of urban land intensive use socio-economic benefits and eco-environmental benefits experienced moderate coordination level and high coordination level.

Q-SA√ESS: a methodology to help solvent selection for pharmaceutical manufacture at the early process development stage

2016 ◽  
Vol 18 (24) ◽  
pp. 6564-6572 ◽  
Author(s):  
Valerio Isoni ◽  
Loretta L. Wong ◽  
Hsien H. Khoo ◽  
Iskandar Halim ◽  
Paul Sharratt
Keyword(s):  
Manufacturing Process ◽  
Process Development ◽  
Development Stage ◽  
Solvent Selection ◽  
Pharmaceutical Manufacture ◽  
Selection For

A practicable, LCA based methodology has been developed to evaluate the sustainability implications of solvent selection during early process development for a batch manufacturing process for an API.

Effect of 2-(4-chlorophenylthio) ethylamine hydrochloride on lycopene biosynthesis in pepper fruits

2020 ◽  
pp. 1-6
Author(s):  
Li Li ◽  
Shi-Lin Tian ◽  
Shi-Lun Xu
Keyword(s):  
Developmental Stages ◽  
Quantitative Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Analysis ◽  
Inhibitory Effect ◽  
Development Stage ◽  
Metabolic Product ◽  
Normal Expression ◽  
Other Substances ◽  
Lycopene Content ◽  
Polymerase Chain

Lycopene is an intermediate metabolic product of the capsanthin biosynthesis pathway in pepper fruits and is one of the strongest antioxidants found in plants. During the ripening of pepper fruits, lycopene is almost completely transformed into the downstream metabolic product capsanthin as well as other substances. As a result, lycopene cannot be enriched in ripe pepper fruits; however, the lycopene content can be increased by 2-(4-chlorophenylthio) ethylamine hydrochloride (CPTA) treatment, using the optimal concentration at the optimal development stage of pepper fruits. The current study tested different CPTA concentrations and fruit developmental stages to increase the lycopene content in pepper fruits. The results showed that the lycopene content was significantly enriched in pepper fruits treated with 0.1% CPTA applied at the turning stage. Real-time quantitative polymerase chain reaction analysis showed that CPTA treatment significantly promoted the expression of the upstream genes (Psy and PDS) involved in the anabolic metabolism of lycopene; however, CPTA treatment had a significant inhibitory effect on the downstream gene (Lcyb) of lycopene synthesis. Therefore, in pepper fruits, CPTA inhibits the normal expression of the Lcyb gene downstream of lycopene, thus achieving notable lycopene enrichment.

scholarly journals Accelerating Biologics Manufacturing by Modeling or: Is Approval under the QbD and PAT Approaches Demanded by Authorities Acceptable Without a Digital-Twin?

Processes ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 94 ◽  
Author(s):  
Steffen Zobel-Roos ◽  
Axel Schmidt ◽  
Fabian Mestmäcker ◽  
Mourad Mouellef ◽  
Maximilian Huter ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Process Development ◽  
Process Analytical Technology ◽  
Control Strategies ◽  
Development Stage ◽  
Point Of View ◽  
Digital Twin ◽  
Market Competitiveness ◽  
Digital Twins ◽  
Potential Methods

Innovative biologics, including cell therapeutics, virus-like particles, exosomes,recombinant proteins, and peptides, seem likely to substitute monoclonal antibodies as the maintherapeutic entities in manufacturing over the next decades. This molecular variety causes agrowing need for a general change of methods as well as mindset in the process development stage,as there are no platform processes available such as those for monoclonal antibodies. Moreover,market competitiveness demands hyper-intensified processes, including accelerated decisionstoward batch or continuous operation of dedicated modular plant concepts. This indicates gaps inprocess comprehension, when operation windows need to be run at the edges of optimization. Inthis editorial, the authors review and assess potential methods and begin discussing possiblesolutions throughout the workflow, from process development through piloting to manufacturingoperation from their point of view and experience. Especially, the state-of-the-art for modeling inred biotechnology is assessed, clarifying differences and applications of statistical, rigorousphysical-chemical based models as well as cost modeling. “Digital-twins” are described and effortsvs. benefits for new applications exemplified, including the regulation-demanded QbD (quality bydesign) and PAT (process analytical technology) approaches towards digitalization or industry 4.0based on advanced process control strategies. Finally, an analysis of the obstacles and possiblesolutions for any successful and efficient industrialization of innovative methods from processdevelopment, through piloting to manufacturing, results in some recommendations. A centralquestion therefore requires attention: Considering that QbD and PAT have been required byauthorities since 2004, can any biologic manufacturing process be approved by the regulatoryagencies without being modeled by a “digital-twin” as part of the filing documentation?

scholarly journals Virtual Thermocouple: A Non-Invasive Multipoint Product Temperature Measurement for Lyophilization

2021 ◽  
Author(s):  
Xiaofan Jiang ◽  
Petr Kazarin ◽  
Michael Sinanis ◽  
Nithin Raghunathan ◽  
Alina Alexeenko ◽  
...  
Keyword(s):  
Temperature Measurement ◽  
Process Development ◽  
Threshold Value ◽  
Development Stage ◽  
Temperature Sensing ◽  
Critical Importance ◽  
Point Temperature ◽  
Product Temperature ◽  
Non Invasive ◽  
Drying Behavior

Abstract Monitoring product temperature during lyophilization is of critical importance, especially during the process development stage, as the final product may be jeopardized if its process temperature exceeds a threshold value. While conventional thermocouples can track product temperature, they are invasive and can significantly alter the freezing and drying behavior. In this work, a new methodology for non-invasive product temperature monitoring and drying behavior during the entire lyophilization process is proposed and experimentally validated. The method is based on a new flexible wireless multi-point temperature sensing probe that is attached to the outside of the vial. Combining the wirelessly-collected data with advanced multi-physics simulations allows the accurate extraction of the product temperature non-invasively.

scholarly journals Biochemical Mechanisms of Mimosine Toxicity to Sclerotium rolfsii Sacco

1983 ◽  
Vol 36 (4) ◽  
pp. 445 ◽  
Author(s):  
Edralina P Serrano ◽  
LinAL Ilag ◽  
Evelyn Mae T Mendoza
Keyword(s):  
Metal Ions ◽  
Mycelial Growth ◽  
Inhibitory Action ◽  
Sclerotium Rolfsii ◽  
Inhibitory Effect ◽  
Final Concentration ◽  
Potato Dextrose Broth ◽  
Biochemical Mechanisms

Mimosine when added at a final concentration of 2�5 mM to potato dextrose broth reduced the mycelial growth of S. rolfsii by 40-60 % and prevented the formation of sclerotia. This inhibitory action of mimosine was alleviated by 80 % by 5 mM pyridoxal-5-phosphate and by 50 % by tryptophan and 5 mM tyrosine. Phenylalanine had little effect. Among the metal ions, Fe3 + at 10 mM most effectively decreased mimosine's inhibitory effect by 93 %, AI3 + at 5 mM decreased it by 80 % while Fe2+ and Cu2 + at 5 mM exhibited the least effect (about 25 and 9 %, respectively).

scholarly journals Optimal Sugar Compostitions And Conditions For Enhanced Biobutanol Bioproduction From Agriculture Residues

2021 ◽  
Author(s):  
Hadeel A. Nedaff
Keyword(s):  
Renewable Resources ◽  
Ph Control ◽  
Butanol Production ◽  
Sugar Composition ◽  
The Past ◽  
Optimization Study ◽  
Agriculture Residues ◽  
Acid Accumulation ◽  
Effective System ◽  
Butanol Concentration

Production of alternative non-fossil biofuels based on renewable resources has been the focus of research in the past few decades due to its environmental and economical advantages. The current study focuses on testing two Clostridia strains towards production of butanol. The work was performed in three parts: the first part includes introducing C acetobutylicum ATCC 4259 for butanol production and identifying the proper working conditions for this strain. The following part includes extending investigation of production to examine C. beijerinckiiBA101 and compare with results obtained from C. acetobutylicum. In the last part, an optimization study was conducted on a presently derived mathematical model in order to predict the best sugar composition in the feedstock for maximum production of butanol. Results showed that the agriculture residues are potential biomass resource for biofuel industry sin both Clostridia strains were successfully able to utilize all types of agricultural sugars including hexose and pentose. However, using C. beijerinkckii resulted in 53% higher butanol concentration than using introduced C. acetobutylicum. The yield was fairly comparable, while high acid accumulation found when using C. acetobutylicum made this strain inapplicable to anaerobic batch fermentation without effective system of pH control.

Exploring How Lean Product and Process Development Can Promote Industrial Sustainability

2021 ◽  
Author(s):  
Daniel R. Cooper ◽  
Katrina Appell
Keyword(s):  
Case Studies ◽  
Manufacturing Industry ◽  
Process Development ◽  
Sustainable Manufacturing ◽  
Cost Savings ◽  
Environmental Benefits ◽  
Value Stream Mapping ◽  
Structured Interviews ◽  
Organizational System ◽  
Product And Process

Abstract Approximately one-third of anthropogenic greenhouse gas emissions from energy and processing are from making products and chemicals. These emissions can be lowered if they are deliberately considered during the design of products. How can lean product and process development (LPPD) be used to build environmental considerations into the product development system? We explore if LPPD is an effective system in delivering sustainable manufacturing. We conducted case studies in the automotive and oil & gas industries in which LPPD was used to deliver significant physical changes to the product and product manufacturing. We completed semi-structured interviews with stakeholders and focused on the motivation for the change, how LPPD was either a help or hindrance, and the delivered cost savings and environmental benefits. Findings from the case studies were used to structure a workshop on LPPD and sustainability held at a leading manufacturing (industry focused) conference. In this workshop, we focused on first identifying the main environmental impacts in each industry, the physical opportunities to reduce those impacts, and how LPPD might help or hinder delivering that change. This article structures the findings from the case studies and workshop. We present a guide (with examples) for how various LPPD methods (e.g., concept papers, value stream mapping, and design guides) might be used to meet sustainability challenges (e.g., reducing the generation of manufacturing scrap). While LPPD is itself agnostic to sustainability, we suggest that it is an effective method of creating an organizational system for promoting sustainable manufacturing particularly in complex environments.

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