scholarly journals Atrial Fibrillation in Dengue Infection: A Self-limiting Phenomenon? (Two Case Reports)

2018 ◽  
Vol 4 (1) ◽  
pp. 36
Author(s):  
Raymond Pranata ◽  
Wendy Wiharja ◽  
Vito Damay
Keyword(s):  
Atrial Fibrillation ◽  
Dengue Virus ◽  
Case Reports ◽  
Dengue Infection ◽  
The Other ◽  
Intravenous Fluids ◽  
Close Monitoring ◽  
Ns1 Antigen ◽  
Ventricular Response ◽  
Electrocardiogram Ecg

Dengue fever (DF) is highly prevalent in Indonesia as evidenced by 129,650 cases in 2015.Atrial fibrillation (AF) in dengue is exceptionally rare and usually self-limiting with resolution after recovery of illness. The aim of this case report is to depict two patients with AF in DF which resolves spontaneously in one and persists after infection in the other. Case 1 was 50 years old male presented with fever since 4 days before admission. NS1 antigen and IgM anti-Dengue virus were positive. An electrocardiogram (ECG) showed AF with rapid ventricular response (AFRVR). Case 2 was 53 years old male presented with dyspnea and palpitations 1 hour before admission. Patient had fever since 5 days before admission. Laboratory exams showed leukopenia, thrombocytopenia and positive IgM anti-Dengue virus. An electrocardiogram showed AFRVR. Intravenous fluids (normal saline), paracetamol, and digoxin were administered in both patients. They were admitted for close monitoring. Pre-discharge ECG of Case 1 showed resolution of AF. However, in Case 2, AF persists in pre-discharge ECG. In conclusion, physicians should be aware that a potentially reversible atrial fibrillation might be caused by this infection. It should be ensured that in those persisting cases, they should not be dismissed as just an ‘irreversible’ AF and progress into full-blown heart failure.

scholarly journals Comparison of Seven Commercial Antigen and Antibody Enzyme-Linked Immunosorbent Assays for Detection of Acute Dengue Infection

2012 ◽  
Vol 19 (5) ◽  
pp. 804-810 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Richard G. Jarman ◽  
Robert V. Gibbons ◽  
Ampai Tanganuchitcharnchai ◽  
Mammen P. Mammen ◽  
...  
Keyword(s):  
South Korea ◽  
Dengue Virus ◽  
Dengue Infection ◽  
Virus Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Capture Elisa ◽  
Igm Antibody ◽  
Antigen Elisa ◽  
Ns1 Antigen ◽  
Elisa Format

ABSTRACTSeven commercial assays were evaluated to determine their suitability for the diagnosis of acute dengue infection: (i) the Panbio dengue virus Pan-E NS1 early enzyme-linked immunosorbent assay (ELISA), second generation (Alere, Australia); (ii) the Panbio dengue virus IgM capture ELISA (Alere, Australia); (iii) the Panbio dengue virus IgG capture ELISA (Alere, Australia); (iv) the Standard Diagnostics dengue virus NS1 antigen ELISA (Standard Diagnostics, South Korea); (v) the Standard Diagnostics dengue virus IgM ELISA (Standard Diagnostics, South Korea); (vi) the Standard Diagnostics dengue virus IgG ELISA (Standard Diagnostics, South Korea); and (vii) the Platelia NS1 antigen ELISA (Bio-Rad, France). Samples from 239 Thai patients confirmed to be dengue virus positive and 98 Sri Lankan patients negative for dengue virus infection were tested. The sensitivities and specificities of the NS1 antigen ELISAs ranged from 45 to 57% and 93 to 100% and those of the IgM antibody ELISAs ranged from 85 to 89% and 88 to 100%, respectively. Combining the NS1 antigen and IgM antibody results from the Standard Diagnostics ELISAs gave the best compromise between sensitivity and specificity (87 and 96%, respectively), as well as providing the best sensitivity for patients presenting at different times after fever onset. The Panbio IgG capture ELISA correctly classified 67% of secondary dengue infection cases. This study provides strong evidence of the value of combining dengue virus antigen- and antibody-based test results in the ELISA format for the diagnosis of acute dengue infection.

scholarly journals NS-1 antigen positive Dengue Infection and molecular characterization of Dengue Viruses in a private Medical College Hospitalin Dhaka, Bangladesh

2018 ◽  
Vol 17 (4) ◽  
pp. 669-673
Author(s):  
Mahmuda Siddiqua ◽  
Ahmed Nawsher Alam ◽  
AKM Muraduzzaman ◽  
Tahmina Shirin
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Infection ◽  
Medical Science ◽  
Cost Effective ◽  
Rt Pcr ◽  
Dengue Virus Infection ◽  
Medical College ◽  
Virus Serotype ◽  
Ns1 Antigen

Introduction: Detection of dengue virus infection as soon as possible is critical for management of dengue virus infected patients. Immuno-chromatographic (ICT) tests are easy, cost effective method for dengue virus antigen detection.The sensitivity and specificity of ICT should compare with a gold standard test like RT-PCR. Aim of this study was to compare two test methods (ICT and RT-PCR), observe dengue serotype and seasonal impact on dengue infection.Methodology & result: The patients of Ibn Sina Medical College Hospital from October 2015 to October 2017 were tested for dengue NS1 antigen by ICT method. Out of 3201 sample tested 32.39% were found positive and 89 of which were re-tested for RT-PCR for comparison. Eighty eight of 89 NS1 positive cases showed positive by RT-PCR method giving an accuracy of 98.87%. Among the RT-PCR positive cases 45 were further analyzed for serotype. DEN-1, DEN-2 or both DEN- 1 and DEN-2 were found in 21, 23 and 1cases respectively. No cases of DEN-3 or DEN-4 were detected.Conclusion: This study showed that easily available and cost effective dengue NS1 antigen detection method (ICT) is as effective as molecular test (RT-PCR). DEN-1 and DEN-2 serotype were prevalent during last few years in Bangladesh. Continuous monitoring of dengue virus serotype is important for prevention and control of sudden epidemic by other serotype. Alert to be more during post monsoon when the peak of dengue virus infection was observed.Bangladesh Journal of Medical Science Vol.17(4) 2018 p.669-673

scholarly journals Congenital Dengue Infection: A Novel Case of Vertical Transmission of Dengue Virus in Karachi, Pakistan

2021 ◽  
Keyword(s):  
Dengue Virus ◽  
Vertical Transmission ◽  
Health Care Providers ◽  
Blood Platelets ◽  
Dengue Infection ◽  
Maculopapular Rash ◽  
Liver Function Tests ◽  
Care Providers ◽  
Antigen Test ◽  
Ns1 Antigen

Dengue is a viral infection that is transmitted generally among humans through mosquito bite. This article reports a less known mode of transmission i.e., vertical transmission of dengue virus. A male newborn with normal Apgar score developed fever on the 8th day of life. The fever progressed along with an erythematous rash. There was no evidence of mosquito exposure to the baby. However, the mother had mosquito bites two days prior to her delivery. The mother also developed fever on seventh day of delivery and a maculopapular rash during her ninth postpartum day. Baseline investigations of both the mother and the neonate showed thrombocytopenia, anemia, raised liver function tests and positive Dengue NS1 antigen test. The diagnosis of dengue fever was established. This case report demonstrates the possibility of congenital transmission of dengue virus. The health care providers should be aware of and adequately trained for handling such cases. Keywords: Dengue; Blood Platelets; Infections; Thrombocytopenia; Antigens.

Intelligent Classifier for Atrial Fibrillation (ECG)

2011 ◽  
pp. 910-916
Author(s):  
O. Valenzuela ◽  
I. Rojas ◽  
F. Rojas ◽  
A. Guillen ◽  
L. J. Herrera ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Cardiac Pace ◽  
Heart Beat ◽  
The Other ◽  
Cardiac Diseases ◽  
Electrical Impulses ◽  
Electrocardiogram Ecg ◽  
The Waves ◽  
Selection Of

This chapter is focused on the analysis and classification of arrhythmias. An arrhythmia is any cardiac pace that is not the typical sinusoidal one due to alterations in the formation and/or transportation of the impulses. In pathological conditions, the depolarization process can be initiated outside the sinoatrial (SA) node and several kinds of extra-systolic or ectopic beatings can appear. Besides, electrical impulses can be blocked, accelerated, deviated by alternate trajectories and can change its origin from one heart beat to the other, thus originating several types of blockings and anomalous connections. In both situations, changes in the signal morphology or in the duration of its waves and intervals can be produced on the ECG, as well as a lack of one of the waves. This work is focused on the development of intelligent classifiers in the area of biomedicine, focusing on the problem of diagnosing cardiac diseases based on the electrocardiogram (ECG), or more precisely on the differentiation of the types of atrial fibrillations. First of all we will study the ECG, and the treatment of the ECG in order to work with it, with this specific pathology. In order to achieve this we will study different ways of elimination, in the best possible way, of any activity that is not caused by the auriculars. We will study and imitate the ECG treatment methodologies and the characteristics extracted from the electrocardiograms that were used by the researchers that obtained the best results in the Physionet Challenge, where the classification of ECG recordings according to the type of Atrial Fibrillation (AF) that they showed, was realised. We will extract a great amount of characteristics, partly those used by these researchers and additional characteristics that we consider to be important for the distinction mentioned before. A new method based on evolutionary algorithms will be used to realise a selection of the most relevant characteristics and to obtain a classifier that will be capable of distinguishing the different types of this pathology.

scholarly journals Trend of Dengue Virus Infection with Seasonal Variation at HIMS, Varanasi, Uttar Pradesh, India

2021 ◽  
Author(s):  
Hari Om Trivedi ◽  
Asim Kumar Singh ◽  
Mukesh Kumar Singh
Keyword(s):  
Platelet Count ◽  
Seasonal Variation ◽  
Dengue Virus ◽  
Dengue Infection ◽  
Uttar Pradesh ◽  
Febrile Illness ◽  
Cerebral Hypoperfusion ◽  
Structural Protein ◽  
Igg Antibody ◽  
Ns1 Antigen

Introduction: Dengue is the most common mosquito borne arboviral infection in India, and it has become endemic in India with consistent episodes. Dengue is an acute febrile illness caused by Dengue Virus (DENV-1, DENV-2, DENV-3, DENV-4), an arthropod of family Flaviviridae, transmitted through the bite of female Aedes aegypti mosquitoes. Although, dengue is more prevalent in humans but also it may sometimes be seen in monkeys. The only animals to be affected when bitten by a mosquito, infected with dengue fever are monkeys other animals do not carry or spread dengue. Patient presents with hyperthermia, headache, severe joint and muscle pain, fatigue and skin rashes. Neurological manifestation in sever dengue results from multiorgan dysfunction due to cerebral edema, haemorrhage due to vascular leak, cerebral hypoperfusion, and electrolyte disturbances. Aim: To study serological markers with association of platelet count and trend of DENV infection with seasonal variation. Materials and Methods: The present retrospective study was conducted for a period of two years in Heritage Institute of Medical Sciences (HIMS), Varanasi, Uttar Pradesh, India, from January 2017 to December 2018. Blood samples were collected for 2140 suspected dengue patients. Out of the serologically positive cases, serological confirmation and platelet count was done for dengue infection. Data was presented as percentages. Results: Total 2140 suspected cases were admitted and out of them 199 (9.3%) were found to be seropositive for dengue infection. Non structural protein 1 (NS1) positive cases out of which total seropositive cases were reported to be 127 (63.81%) and 147 (73.86%) cases were positive for NS1 antigen either alone or along with antibody. Out of the total 199 positive cases thrombocytopenia was observed in 126 (63.31%) cases. Positive cases which were under 15 years of age were reported to be 92 (46.23%). Conclusion: The study concludes that NS1 antigen and IgM- IgG antibody consideration in the diagnosis of dengue infection builds the opportunity of early diagnosis so as to keep away the complications significantly.

scholarly journals Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Laura Mónica Álvarez-Rodríguez ◽  
Angel Ramos-Ligonio ◽  
José Luis Rosales-Encina ◽  
María Teresa Martínez-Cázares ◽  
Aurora Parissi-Crivelli ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Fusion Proteins ◽  
Protective Antigen ◽  
Detection System ◽  
Dengue Infection ◽  
Serum Samples ◽  
Diagnostic Potential ◽  
Ns3 Protein ◽  
Ns1 Antigen ◽  
Commercial Kits

Dengue is one of the major public health concerns in the world. Since all the four serotypes are actively circulating in Mexico, there is a need to develop an efficient diagnosis system to improve case management of the patients. There exist few studies evaluating the use of the NS3 protein as a protective antigen against dengue virus (DENV). In this paper we show the expression of a recombinant NS3 protein from all serotypes of dengue virus (GST-DVNS3-1-4) and report a reliable “in-house detection system” for the diagnosis of dengue infection which was field-tested in a small village (Tezonapa) in the state of Veracruz, Mexico. The fusion proteins were immunogenic, inducing antibodies to be able to recognize to antigens up to a 1 : 3200 dilution. The purified proteins were used to develop an in-house detection system (ELISA) and were further tested with a panel of 239 serum samples. The in-house results were in excellent agreement with the commercial kits withκ=0.934±0.064(95%  CI = 0.808–1.061), andκ=0.872±0.048(95%  CI = 0.779–0.965) for IgM and IgG, respectively. The agreement between the NS1 antigen detection versus the rNS3 ELISA,κ=0.837±0.066(95%  CI = 0.708–0.966), was very good. Thus, these results demonstrate that recombinant NS3 proteins have potential in early diagnosis of dengue infections.

scholarly journals Comparative evaluation of dengue NS1 antigen rapid test and IgM ELISA in a tertiary care hospital in VIMS, Ballari

2021 ◽  
Vol 7 (3) ◽  
pp. 175-178
Author(s):  
Gajendra Varma
Keyword(s):  
Dengue Virus ◽  
Rapid Method ◽  
Dengue Infection ◽  
Rapid Test ◽  
Antigen Detection ◽  
Care Hospital ◽  
Age Group ◽  
Igm Elisa ◽  
Test Kit ◽  
Ns1 Antigen

Dengue virus infection is becoming a major public health problem in recent decades because of the mortality and morbidity associated with it. Dengue is endemic in many parts of India and epidemics are frequently reported from various parts of India and abroad. The mortality rate in patients with dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) is as high as 44%. Hence early and rapid laboratory diagnosis of dengue is crucial. Appropriate clinical management can save the lives of DHF and DSS patients and mortality can be reduced to less than 1%. It is also worthwhile for planning appropriate control strategies. The present communication reports the seroprevalence of dengue infections occurred in Central India.To detect Dengue infection by NS1 Antigen Rapid detection and by IgM ELISA and compare Rapid NS1 Antigen detection test and IgM ELISA test.Blood samples from 5273 suspected cases were collected from January 2016 to December 2016. Sera were separated and subjected for NS1 antigen detection testing by the solid phase immunochromatographic assay, a commercial dengue virus rapid test kit. and antidengue IgM antibody ELISA in the Department of Microbiology, VIMS, Ballari. All samples were put for ELISA.3036 samples were tested for NS1 Antigen detection rapid method.2272 samples were tested for both rapid test and ELISA. Out of 5273 cases, 446 (8.46%) were found to be positive by IgM ELISA. 3036 samples WHICH were tested for NS1 Antigen detection by rapid method 208 (6.85%)samples were positive. The most affected age group was 5-10yrs with 284 cases, out of which 29 were positive, followed by the age group 0f 15yrs and above with 8.74%. Males were affected more than females with a percentage of 9.2 and 7.4 respectively. The highest number of suspected dengue patients admitted was in the month of November, i.e., 459 with 68 positive (14.81%) followed by August (12.16%) and September (11.03%), i.e., 28 with 7 (25%) positive. For the 2272 samples for which both tests were done, 153 cases were positive for rapid test and 137 cases were positive by ELISA. Dengue cases were more during September to November in the post monsoon season which is useful to plan special preventive strategies. The study draws attention toward the male, young and adult age group. To conclude, in countries lacking infrastructure for the diagnostic labs especially in the rural and remote areas, the rapid dengue ICT tests can play a major role in diagnosis and in patient management of acute dengue infection. The rapid ICTs are very simple, easy to perform, and can be used as point of care tests. We suggest that the rapid ICT for NS1 Ag detection may be used in patients presenting with fever less than 5 days

scholarly journals DISTRIBUSI SEROTIPE DENGUE DI SURABAYA TAHUN 2012

2016 ◽  
Vol 19 (1) ◽  
pp. 41 ◽  
Author(s):  
Aryati Aryati ◽  
Puspa Wardhani ◽  
Benediktus Yohan ◽  
Eduardus Bimo Aksono H ◽  
R. Tedjo Sasmono
Keyword(s):  
Dengue Virus ◽  
Dengue Infection ◽  
Laboratory Diagnosis ◽  
Multiple Infections ◽  
Annual Change ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Dengue Disease ◽  
Denv Serotypes ◽  
Ns1 Antigen

The characteristics of epidemic dengue often presented as periods of hyperendemicity or as the co-circulation of multiple dengue serotypes. Surabaya is an endemic city for Dengue virus (DENV) transmission. Previous study of DENV distribution in 2008-2009 revealed the predominance of DENV-2. DENV serotypes distribution is known to be dynamic and serotype predominance may change through time. This study aims to determine and follow the circulation of DENV serotype in Surabaya in 2012. We recruited 154 denguesuspected patients attending Dr. Soetomo Hospital during February until August 2012. Dengue cases were confirmed by IgG and IgM serology tests and NS1 antigen detection. Serologically-positive samples were further analyzed using two-steps reverse transcriptasepolymerase chain reaction (RT-PCR) and viruses were isolated by propagation in C6/36 mosquito cell line. Seventy one cases (46.1%) were detected as DENV positive infection. Serotyping revealed that 61 samples have monotypic infection with one of all four of DENV serotypes and 10 samples have mix-infections. Overall serotyping result observed the predominance of DENV-1 (60.56%). Our result revealed the circulation of all four serotypes of DENV and the presence of serotype exchange in Surabaya in 2012. Annual change of predominant serotype and the presence of multiple infections may play an important role in the transmission of dengue infection. This information is valuable to dengue surveillance in the region. Therefore, the laboratory diagnosis of DENV serotype should be routinely performed to follow the dynamic of dengue disease

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