scholarly journals The Role of Turnera subulata and Cosmos sulphureus Flowers in the Life of Anagrus nilaparvatae (Hymenoptera: Mymaridae)

2018 ◽  
Vol 22 (1) ◽  
pp. 43 ◽  
Author(s):  
Wiwik Sugiharti ◽  
Y. Andi Trisyono ◽  
Edhi Martono ◽  
Witjaksono Witjaksono

Anagrus nilaparvatae is a potential egg parasitoid to control the rice brown planthopper (Nilaparvata lugens Stal.) The parasitoid needs to consume suitable food to maximize its biotic potential and parasitizing ability. This study was aimed to determine the benefits provided by the presence of Turnera subulata and Cosmos sulphureus flowers on the life of A. nilaparvatae. This study consisted of two experiments. The first experiment was designed to determine the effects of the tested flowers on the parasitism and hatching rate of A. nilaparvatae on N. lugens eggs. The flowers were set inside the rearing cage of parasitoid in the presence of N. lugens eggs in Ciherang rice seedlings. In addition, honey and the control (no feed added) were included into the treatments, totalling of four treatments. The parasitism rate of A. nilaparvatae fed with the flowers or honey was similar to those unfed. However, the hatching rate of A. nilaparvatae was much higher on those fed with flower or honey than those unfed. The number of A. nilaparvatae unable to eclose from eggs of N. lugens for the unfed parasitoid was 37.4% in comparison with 8.19 to 15.67% for those fed with flower or honey. The second experiment was a follow-up to address the question on the fitness of progeny of A. nilaparvatae fed with the tested flowers. The flowers and honey did not increase the longevity of A. nilaparvatae progeny. However, A. nilaparvatae fed with flowers or honey produced progeny that resulted in higher number of offspring compared to those unfed. C. sulphureus flower significantly increased the number of offspring produced by A. nilaparvatae. This suggest that the diet of the parental parasitoid determines the fitness of the progeny. Improving the hatching rate and the fecundity of progeny produced by the adults of A. nilaparvatae fed with the flower of T. subulata and C. sulphureus would contribute to the increasing population of this parasitoid which could lead to a better control of N. lugens in the rice field. IntisariAnagrus nilaparvatae merupakan parasitoid telur yang potensial untuk mengendalikan serangan wereng batang cokelat (Nilaparvata lugens Stal.). Parasitoid perlu mendapatkan sumber pakan yang sesuai untuk memaksimalkan potensi biologis dan kemampuan memarasit inang. Penelitian ini bertujuan untuk mengkaji manfaat pemberian bunga Turnera subulata dan Cosmos sulphureus pada kehidupan A. nilaparvatae. Penelitian ini terdiri dari dua percobaan. Percobaan pertama dirancang untuk mengetahui pengaruh bunga yang diuji terhadap tingkat parasitasi dan penetasan telur A. nilaparvatae pada telur N. lugens. Bunga diletakkan di dalam tabung rearing parasitoid yang di dalamnya terdapat bibit padi varietas Ciherang yang mengandung telur N. lugens. Selain itu, terdapat pula perlakuan madu dan kontrol (tanpa bunga dan tanpa madu), dengan total empat perlakuan. A. nilaparvate yang diberi pakan bunga atau madu memiliki tingkat parasitasi yang sama dengan yang tidak diberi pakan. Namun, tingkat penetasan telur A. nilaparvatae yang diberi pakan bunga atau madu jauh lebih tinggi daripada yang tidak diberi pakan. Jumlah A. nilaparvatae yang tidak menetas dari telur N. lugens pada parasitoid yang tidak diberi pakan adalah 37,4% dibandingkan dengan parasitoid yang diberi pakan bunga atau madu yang berkisar 8,19 - 15,67%. Percobaan kedua adalah tindak lanjut untuk menjawab pertanyaan tentang kebugaran keturunan A. nilaparvatae yang diberi pakan dengan bunga yang diuji. Bunga dan madu tidak meningkatkan lama hidup keturunan A. nilaparvatae. Namun, A. nilaparvatae yang diberi pakan dengan bunga atau madu menghasilkan keturunan yang memiliki fekunditas lebih tinggi dibandingkan dengan keturunan dari A. nilaparvatae yang tidak diberi pakan. Bunga C. sulphureus secara signifikan mampu meningkatkan jumlah keturunan yang dihasilkan oleh A. nilaparvatae. Ini menunjukkan bahwa jenis pakan induk parasitoid menentukan kebugaran keturunannya. Peningkatan penetasan dan fekunditas dari keturunan yang dihasilkan oleh induk A. nilaparvatae yang diberi pakan bunga T. subulata dan C. sulphureus akan berkontribusi pada peningkatan populasi parasitoid sehingga dapat mengendalikan serangan N. lugens di pertanaman padi dengan lebih baik.

2019 ◽  
Vol 23 (1) ◽  
pp. 68
Author(s):  
Nike Grace Hanjelina Sinulingga ◽  
Y. Andi Trisyono ◽  
Edhi Martono ◽  
Buyung Hadi

Outbreaks of the rice brown planthopper, Nilaparvata lugens Stal., tend to increase in 2017. There has been significant interest to reduce reliance on pesticides by manipulating habitat plant species and communities to benefit natural enemies of insect. Flowering plants as refuge can contribute in enhancing the ecosystem services. This study aimed to assess the benefit of flowering plants as refuge to improve the role of egg parasitoids of brown planthopper. We sampled three rice fields: rice field adjacent to refuge, far from refuge, and rice field with no refuge using trapping procedure. We found two genera of parasitoid in Banyumas: Oligosita and Anagrus. The parasitism in the three rice fields was 46.14, 43.05 and 42.32%, respectively, showing no differences. However, the number of parasitoids emerged from the traps placed in the rice field with refuge was higher (31.08 adults/trap) than the other two rice fields (25.67 and 20.71 adults/trap). In addition, the number of unhatched parasitoids was lower in the rice with refuge (5.9%) compared to no refuge (14.54%). These findings show that the refuge provides better environments for the parasitoid by improving the number of progeny produced which eventually could increase their role in managing N. lugens population.


2021 ◽  
Vol 12 ◽  
Author(s):  
Xiao-Xiao Shi ◽  
Mu-Fei Zhu ◽  
Ni Wang ◽  
Yuan-Jie Huang ◽  
Min-Jing Zhang ◽  
...  

Ceramides are bioactive sphingolipids that have been implicated in insect development; however, their role in insect reproduction remains poorly understood. Here, we report the pivotal role of neutral ceramidase (NCER) in the female reproduction of the brown planthopper (BPH), Nilaparvata lugens (Stål), a significant pest in rice cultivation in Asia. LC-MS/MS demonstrated that, among different developmental stages of BPH, the levels of ceramides were highest in 1st instar nymphs and lowest in adults. The transcription of NCER was negatively correlated with the levels of ceramides at different developmental stages of BPH, in that the transcript levels of NCER were the highest, whereas ceramides levels were the lowest in BPH adults. Knocking down NCER through RNA interference (RNAi) increased the levels of ceramides in BPH females and ovaries, which resulted in a delay in oocyte maturation, a reduction in oviposition and egg hatching rate, as well as the production of vulnerable offspring. Transmission electron microscopy (TEM) analysis and TdT-mediated dUTP Nick-End Labeling (TUNEL) assays showed mitochondrial deficiency and apoptosis in NCER-deficient oocytes. Taken together, these results suggest that NCER plays a crucial role in female reproduction in BPH, likely by regulating the levels of ceramides.


Insects ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 908
Author(s):  
Finbarr G. Horgan ◽  
Ainara Peñalver Cruz ◽  
Arriza Arida ◽  
Jedeliza B. Ferrater ◽  
Carmencita C. Bernal

The adaptation by planthoppers to feed and develop on resistant rice is a challenge for pest management in Asia. We conducted a series of manipulative experiments with the brown planthopper (Nilaparvata lugens (Stål)) on the resistant rice variety IR62 (BPH3/BPH32 genes) to assess behavioral and bionomic changes in planthoppers exhibiting virulence adaptation. We also examined the potential role of yeast-like symbionts (YLS) in virulence adaptation by assessing progeny fitness (survival × reproduction) following controlled matings between virulent males or females and avirulent males or females, and by manipulating YLS densities in progeny through heat treatment. We found virulence-adapted planthoppers developed faster, grew larger, had adults that survived for longer, had female-biased progeny, and produced more eggs than non-selected planthoppers on the resistant variety. However, feeding capacity—as revealed through honeydew composition—remained inefficient on IR62, even after 20+ generations of exposure to the resistant host. Virulence was derived from both the male and female parents; however, females contributed more than males to progeny virulence. We found that YLS are essential for normal planthopper development and densities are highest in virulent nymphs feeding on the resistant host; however, we found only weak evidence that YLS densities contributed more to virulence. Virulence against IR62 in the brown planthopper, therefore, involves a complex of traits that encompass a series of behavioral, physiological, and genetic mechanisms, some of which are determined only by the female parent.


2021 ◽  
Vol 1 (2) ◽  
pp. 45-48
Author(s):  
Darmawan Suryo Sudarsono

A research to observe the population density of brown planthoppers (Nilaparvata lugens, Stal.) and their predator and parasitoid natural enemies has done on the rice field in Bantul regency of Yogyakarta. The research was conducted to observe the hoppers and its natural enemies on three local varieties and one superior brown planthoppers resistant variety of rice. The three local varieties of rice were Rejosari, Sri Kuning, and Sri Ayu, while the hopper resistant variety was IR 64. The observation were replicated three times. The result showed that population of brown planthoppers in the three local varieties of rice were higher than in the resistant IR 64 hybrid variety. The population of predators and parasitoid were relatively high in order to control the development of brown planthopper population. The predator Lycosa sp. and the parasitoids Anagrus sp.  were commonly found in the ecosystem.


Agrikultura ◽  
2015 ◽  
Vol 26 (2) ◽  
Author(s):  
Martua Suhunan Sianipar ◽  
Luciana Djaya ◽  
Entun Santosa ◽  
RC Hidayat Soesilohadi ◽  
Wahyu Daradjat Natawigena ◽  
...  

ABSTRACTPopulation of Brown Plant Hopper (Nilaparvata lugens Stal.) and the Diversity of Its Natural Enemyin Highland Paddy Rice Field in the Village of Panyocokan, Ciwidey District, Bandung RegencyBrown lanthopper/BPH (Nilaparvata lugens Stal.) is a main pest of rice in the field. This researchwas aimed to study the population of BPH and the diversity of its natural enemy in highland paddyrice field. The survey was conducted in three paddy plots (15 m x 20 m) located in the village ofPanyocokan, Ciwidey District, Bandung Regency, West Java. The experiment was conducted in thegreenhouse of the Department of Plant Pests and Diseases, Faculty of Agriculture, UniversitasPadjadjaran. The samples of insects were collected systematically using yellow trap and net trap,and visually observed in each rice cluster. The number of BPH were recorded and accumulatedeach week. Fecundity, life cycle, and sex ratio of the new generation of BPH were observed in 3replications by using 1 pair of WBC on each replication. The result showed that the population ofBPH in the highland was below 10, which means that BPH population was still below theeconomic threshold and control threshold. Temperature, humidity, and rainfall did notsignificantly affect the BPH population. This was indicated by the regression analysis of eachtemperature (Y = 0.557-8.167x; R2 = 0.039; P = 0.465), humidity (Y = -0.077+ 9.112x; R2 = 0.045; P =0.428), and rainfall (Y = -0.118 + 3.412x; R2 = 0.136; P = 0.159). Natural enemy diversity indextended to fluctuate widely from low to moderate. In the temperature range from 21.1°C to 34.8°C,BPH could produce 127-207 new generation during its lifetime. BPH needed an average of 37.66days to produce a new generation. The ratio of male to female was 1.06 : 1.Keywords: population, brown planthopper, diversity, predator, highland paddy rice field, Ciwidey,BandungABSTRAKHama wereng batang cklat/WBC (Nilaparvata lugens Stal.) merupakan hama utama tanaman padi.Penelitian ini bertujuan untuk mempelajari populasi WBC dan keragaman musuh alaminya padatanaman padi sawah di dataran tinggi. Penelitian dilakukan dengan metode survei dan eksperimen.Survei dilakukan pada 3 petak lahan percobaan berukuran 15 mx 20 m bertempat di DesaPanyocokan, Kecamatan Ciwidey, Kabupaten Bandung dan eksperimen dilakukan di rumah kacaDepartemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran. Metodepengambilan sampel dilakukan secara diagonal sistematis. Populasi WBC ditentukan denganmenggunakan perangkap kuning dan perangkap jaring dan dengan pengamatan langsung padarumpun padi. Eksperimen dilakukan dengan meletakkan sepasang WBC dalam wadah, lalu diamatikeperidian, siklus hidup, dan sex ratio keturunannya. WBC yang tertangkap dihitung jumlahnyadan diakumulasikan setiap minggunya. Hasil penelitian menunjukkan bahwa kepadatan populasi dilahan survey di bawah 10 ekor/rumpun yang artinya populasi WBC masih di bawah ambangekonomi atau ambang kendali. Suhu, kelembaban, dan curah hujan tidak memberikan pengaruhyang signifikan terhadap populasi WBC. Hal tersebut ditunjukkan dengan analisis regresi masingmasingpada suhu (Y= 0,557 – 8,167x; R2 = 0,039; P= 0,465), kelembaban (Y = -0,077 + 9,112x; R2 =0,045; P = 0,428), dan curah hujan (Y = -0,118 + 3,412x; R2 = 0,136; P = 0,159). Indeks keragamanmusuh alami cenderung mengalami fluktuasi dari rendah sampai sedang. Pada kisaran suhu 21,1oC–34,8oC, hasil pengamatan keperidian menunjukkan WBC dapat menghasilkan 127-207 individubaru selama masa hidupnya. Pada pengamatan siklus hidup, WBC memerlukan rata-rata 37,66 harisampai menghasilkan generasi baru. Pengamatan sex ratio menunjukkan perbandingan (jantan :betina) 1,06 :1.Kata kunci: populasi, wereng batang coklat, keragaman, predator, sawah, dataran tinggi, Ciwidey,Bandung


2020 ◽  
Vol 24 (1) ◽  
pp. 1
Author(s):  
Nurullah Asep Abdilah ◽  
Hadi Susilo

An extensive study about egg parasitoid biodiversity in different plant growth stages is an important need. This study was aimed to determine the diversity and abundance of egg parasitoids Nilaparvata lugens on different rice growth stages in Saketi, Pandeglang, Banten. The parasitoids sample was collected three times with two days interval sampling in three villages, i.e. Sodong (location I), Sindanghayu (location II), and Sukalangu (location III) from February to September 2018. Ten plants (as bait: rice that infected by N. lugens eggs) were put during two days along the transect line (100 meters) with 10 m space in between. The findings revealed that the abundance richness (S), the diversity index (H'), and similarity (J') of egg parasitoids of N. lugens were higher in the location I than those in the locations II and III. The study indicated that the diversity and abundance of egg parasitoids of N. lugens decreased in the generative phase of rice growth.


Agrikultura ◽  
2016 ◽  
Vol 27 (3) ◽  
Author(s):  
Muhammad Subandi ◽  
Liberty Chaidir ◽  
Ulfah Nurjanah

ABSTRACTEffectiveness of BPMC insecticide and suren leaf extract on brown planthopper (Nilaparvata lugens Stal.) and its natural enemy population on Ciherang rice varietyEffectiveness study of BPMC insecticide and suren leaf extract esticide against brown planthopper and its side-effect on its natural enemy population on paddy Ciherang variety was conducted in rice field of Balai Besar Peramalan Organisme Pengganggu Tumbuhan (BBPOPT), Karawang, West Java from May to August 2015. The experimental design used was Randomized Blok Design with 7 treatments and 4 replications ie suren leaf extract of 50 g/l, 100 g/l and 150 g/l, three concentrations of BPMC of 0.5 ml/l, 1.0 ml/l and 1.5 ml/l, and a control treatment. The results showed that BPMC insecticide and suren leaf extract were able to suppress the population of brown planthopper and influenced its natural enemy population. The treatment of 50 g/l suren leaf extract gave the highest suppression of the brown planthopper population. It was also caused no negative impact to the natural enemies of P. fuscipes and L. pseudoannulata. The application of 1.5 ml/l of BPMC insecticide gave the highest grain weight average of 37.80 kg/plot compared to the lowest production in control treatment of 32.63 kg/plot. Therefore, application of BPMC insecticide and suren leaf extract effectively suppressed the brown planthopper population and the natural enemy existence was assumed to affect the rice grain production in this study.Keywords: BPMC insecticide, Natural enemy, Suren, PlanthopperABSTRAKStudi keefektifan insektisida BPMC dan ekstrak daun suren terhadap hama wereng batang coklat (WBC) dan efek samping terhadap musuh alami wereng pada padi varietas Ciherang dilaksanakan di sawah Balai Besar Peramalan Organisme Pengganggu Tumbuhan (BBPOPT) Karawang mulai Mei 2015 sampai Agustus 2015. Rancangan yang digunakan Rancangan Acak Kelompok (RAK) dengan 7 perlakuan dan 4 ulangan yaitu perlakuan tanpa insektisida (kontrol), 50 g/l ekstrak daun suren, 100 g/l ekstrak daun suren, 150 g/l ekstrak daun suren, 0,5 ml/l insektisida BPMC, 1,0 ml/l insektisida BPMC, dan 1,5 ml/l insektisida BPMC. Hasil menunjukkan perlakuan insektisida BPMC dan ekstrak daun suren dapat menekan populasi hama WBC dan memengaruhi populasi musuh alaminya (Paederus fuscipes dan Lycosa pseudoannulata). Perlakuan ekstrak daun suren konsentrasi 50 g/l dapat menekan populasi WBC tertinggi. Aplikasi ekstrak daun suren tidak berdampak negatif terhadap musuh alami P. fuscipes dan L. pseudoannulata. Perlakuan 1,5 ml/l insektisida BPMC menghasilkan produksi gabah tertinggi dari semua perlakuan dengan rata-rata bobot basah gabah 37,80 kg/petak dibandingkan dengan hasil terendah pada perlakuan kontrol sebesar 32,63 kg/petak. Dengan demikian, aplikasi insektisida BPMC dan ekstrak daun suren berpengaruh terhadap populasi hama WBC dan efek samping dari tingkat keberadaan musuh alami berakibat pada hasil gabah tanaman padi.Kata Kunci: Insektisida BPMC, Musuh alami, Suren, Wereng


2018 ◽  
Vol 22 (2) ◽  
pp. 132
Author(s):  
Endang Warih Minarni ◽  
Agus Suyanto ◽  
Kartini Kartini

This study aims to determine the type and potency of egg parasitoid in controlling brown planthopper (Nilaparvata lugens Stal.) pests in Banyumas regency after the pest’s explosion. This research has been conducted in five districts of the endemic area of brown planthopper in Banyumas Regency, i.e. in  Jatilawang, Cilongok, Kebasen, Sumpiuh, and Kembaran. Each of the  districts was taken 5 sample villages. The testing and calculation of the level of parasitization  were done in the laboratory of Plant Protection, Faculty of Agriculture, University of Jenderal Soedirman, Purwokerto. The research  used nest plot design, where  the first factor was the district and the second factor was the village. Village nested in  district. The data were  analyzed using F 5% test,  followed by 5% DMRT, if there were  any differences found . The results of the study were as follows: (1) The parasitoids found in Banyumas Regency were Gonatocerus sp. and Oligosita sp. with the ability to parasite 26.8−64.73%, and 1.82−31.40%; (2) the presence parasitoid has the potency  to suppress the intensity of brown planthopper attack on the vegetative phase, the intensity of attacks ranged between 6.96−23.58%, with brown planthopper population ranged from 0.84 to 27.36 individuals per hill. IntisariPenelitian ini bertujuan untuk mengetahui jenis dan potensi parasitoid telur dalam mengendalikan hama wereng batang coklat (Nilaparvata lugens Stal.) di Kabupaten Banyumas pasca terjadinya ledakan. Penelitian ini dilaksanakan di lima kecamatan daerah endemik wereng batang cokelat di wilayah Kabupaten Banyumas yaitu Kecamatan Jatilawang, Cilongok, Kebasen, Sumpiuh, Kembaran. Masing-masing kecamatan diambil 5 desa sampel. Pengujian dan penghitungan tingkat pemarasitan dilakukan di laboratorium Perlindungan Tanaman Fakultas Pertanian, Universitas Jenderal Soedirman, Purwokerto. Penelitian menggunakan rancangan petak tersarang,  dengan kecamatan sebagai faktor pertama  dan desa sebagai faktor kedua. Desa tersarang pada kecamatan. Data dianalisis menggunakan uji F 5%, apabila ada perbedaan dilanjutkan dengan uji banding ganda DMRT 5 %. Adapun hasil penelitian adalah sebagai berikut: (1) Parasitoid yang ditemukan di Kabupaten Banyumas adalah Gonatocerus sp. dan Oligosita sp. dengan kemampuan memarasit 26,8−64,73%, dan sebesar 1,82−31,40 %, (2) keberadaan parasitoid berpotensi menekan intensitas serangan hama wereng batang cokelat pada fase vegetatif, intensitas serangan berkisar antara 6,96−23,58%, dengan populasi wereng batang cokelat berkisar 0,84−27,36 individu per rumpun.


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