Effectiveness of Secondary Metabolites from Entomopathogenic Fungi for Control Nilaparvata lugens Stål. in the Laboratory Scale

Nilaparvata lugens Stål. is an essential pest in rice plants. This pest attack can reduce crop yields and even crop failure. This research was conducted to obtain secondary metabolites that are effective in controlling brown planthopper (BPH). A randomized block design was used to test the effectiveness of secondary metabolites against BPH. The treatments tested were secondary metabolites produced by eight isolates of fungi consist of three concentrations: 5, 10, and 15%. Water and imidacloprid insecticide were used as control. The eight isolates were: J11 (Aspergillus sp.), J22 (Lecanicillium saksenae), J34 (Myrothecium sp.), J35 (Beauveria sp.), J41 (Fusarium sp.), J56 (Fusarium sp), J60 (Simplicillium sp.), and J65 (Curvularia sp.). Each treatment was repeated three times. The variables observed were mortality and time of death of BPH. Data were analyzed using the F test and followed by a DMRT if significant differences existed. The results showed that the secondary metabolites of the Lecanicillium saksenae, Myrothecium sp., and Simplicillium sp. fungi effectively controlled BPH pests by 80‒100% within 3.22‒5.47 days. The fungus L. saksenae, Myrothecium sp., and Simplicillium sp. contain insecticidal compounds, clogging the insect spiraculum, antifeedant, repellant, and antimicrobial.

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Effect of Anredera cordifolia (Ten) Steenis Leaves Ethanol Extract in suppressing Brown PlantHopper (Nilaparvata lugens Stal.) Populations on Rice Plant

CROPSAVER - Journal of Plant Protection ◽

10.24198/cropsaver.v3i2.29448 ◽

2020 ◽

Vol 3 (2) ◽

pp. 42

Author(s):

Martua Suhunan Sianipar ◽

Tarkus Suganda ◽

Azhhar Hadyarrahman

Keyword(s):

Rice Plant ◽

Block Design ◽

Brown Planthopper ◽

Ethanol Extract ◽

Nilaparvata Lugens ◽

Botanical Insecticide ◽

Crop Failure ◽

Extract Concentration ◽

Randomized Block Design ◽

Nilaparvata Lugens Stål

The Brown Planthopper (Nilaparvata lugens Stal.) is one of the pests that often causes rice crop failure in rice. One way to control N. lugens pests is to use of botanical insecticide. The purpose of this experiment is to study the effect of A. cordifolia leaves ethanol in suppressing N. lugens populations. The experiment used a Randomized Block Design (RDB) with 8 treatments and 4 replications. The A. cordifolia leaves ethanol extract concentration tested in this experiment consisted of 0,5%; 0,75%; 1%; 1,5%; and 2%. The results of the experiment showed that the ethanol extract of binahong leaves with a concentration of 1%, 1.5% and 2% cause mortality of N. lugens by 52.50%, 56.25%, and 61.25% on the 14th day after application. Based on the results of this experiment, the ethanol extract of A.cordifolia leaves was not effective against N. lugen.

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EVALUASI VARIETAS PADI SAWAH PADA DISPLAY VARIETAS UNGGUL BARU (VUB) DI KABUPATEN KARAWANG, JAWA BARAT

Agric ◽

10.24246/agric.2015.v27.i1.p1-7 ◽

2016 ◽

Vol 27 (1) ◽

pp. 1

Author(s):

Wage Ratna Rohaeni ◽

M Iskandar Ishaq

Keyword(s):

Block Design ◽

Brown Planthopper ◽

Crop Failure ◽

New Variety ◽

Early Maturity ◽

Randomized Block Design ◽

Display Area ◽

New Varieties ◽

High Yielding Varieties ◽

The One

<p>More than 80 high yielding varieties of rice have been released nationally by IAARD to support acceleration of national rice production. Inpari 13 was the one of some new variety that has been released at 2011 and have superior trait i.e early maturity and high yielding. Inpari 13 and some another new variety would disseminated at ICM program at this year. That variety needed to evaluated on Display Area and cultivated by using ICM technology and its compared with old variety as well as Ciherang. This study was to evaluate performance and yield of some new varieties compared old varieties specifically in Dem area. The experiment was conducted in West Java AIAT Dem Area, District Jayakerta, Karawang. The study was done on DS 2011 (Mei 2011-Januari 2012) by using randomized block design with 3 replications. Six varieties were tested i.e Ciherang, St. Bagendit, Inpari Inpari-7, Inpari-10, Silugunggo and Inpari-13. Integrated Crop Manajemen system i.e legowo 2:1, optimal fertilization, and IPM were used at this research. The results showed that there were signifant differences between varieties in all characters except leaf color index. Ciherang has the highest plant height (97,59 cm). Inpari-7 has the highest number of productive tillers and 100 grains weight (25 tillers/clump and 2,8 grams) and the least number of empty grains (7 grains/ panicle). St. Bagendit has the highest number of pithy grain (183 grains/panicle). Silugonggo was the early maturity but it’s resistant to stems brown planthopper (almost had a crop failure). The highest dry grain harvest was owned by Situ Bagendit (8,10 t.ha 1) followed Ciherang (8,08 t.ha-1), Inpari-13 (8,07 t.ha-1), Inpari-7 (7,52 t.ha-1), Inpari-10 (6,71 t.ha-1), and Silugonggo (2,05 t.ha-1).</p>

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Menguji Tingkat Serangan Wereng Batang Coklat Nilaparvata lugens Stal (Hemiptera: Delphacidae) pada Varietas Padi Asal Pasaman di Rumah Kaca

JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) ◽

10.25077/jpt.5.1.55-59.2021 ◽

2021 ◽

Vol 5 (1) ◽

pp. 55

Author(s):

Eva Zulaikha ◽

Arneti Arneti ◽

Munzir Busniah

Keyword(s):

Attack Rate ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Crop Failure ◽

Rice Varieties ◽

Randomized Design ◽

Major Pest ◽

Completely Randomized Design ◽

Nilaparvata Lugens Stål ◽

And Control

Brown planthopper (BPH) is a major pest on rice plants that can cause hopperburn and crop failure. The study aimed to determine the level of BPH attack on several local rice varieties in the Pasaman Regency. This research was conducted in a greenhouse, Faculty of Agriculture, Universitas Andalas, using a completely randomized design (CRD), with five treatments and five replications. The treatments were four different rice varieties from Pasaman and control (Siganteng, Pulau Batu, Mundam Kuning, Mundam Putih, IR 42). BPH was infested when the rice was five days after sowing. Observation parameters were the percentage and the intensity of the attacks. Observations were done until 15 days after infestation; after the IR 42 variety died 90%. The results showed that the attack rate of BPH on rice varieties from Pasaman was moderate. The lowest percentage of attack occurred in the Mundam Putih variety (94%), while the intensity of the attack was not significantly different from other varieties.

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Effects of two climate change factors, elevated temperature and water stress, on the rice brown planthopper,Nilaparvata lugens(Stål)

10.1603/ice.2016.109878 ◽

2016 ◽

Author(s):

Ye Tan

Keyword(s):

Climate Change ◽

Water Stress ◽

Elevated Temperature ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Change Factors ◽

Nilaparvata Lugens Stål

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Preliminary survey of the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) on different varieties of rice and its natural enemies in Central Thailand

Agriculture and Natural Resources ◽

10.34044/j.anres.2019.53.4.12 ◽

2019 ◽

Keyword(s):

Natural Enemies ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Preliminary Survey ◽

Nilaparvata Lugens Stål ◽

Central Thailand

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Heat Stress Impedes Development and Lowers Fecundity of the Brown Planthopper Nilaparvata lugens (Stål)

PLoS ONE ◽

10.1371/journal.pone.0047413 ◽

2012 ◽

Vol 7 (10) ◽

pp. e47413 ◽

Cited By ~ 20

Author(s):

Jiranan Piyaphongkul ◽

Jeremy Pritchard ◽

Jeff Bale

Keyword(s):

Heat Stress ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Nilaparvata Lugens Stål

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RNA interference-aided knockdown of a putative saccharopine dehydrogenase leads to abnormal ecdysis in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

Bulletin of Entomological Research ◽

10.1017/s0007485315000231 ◽

2015 ◽

Vol 105 (4) ◽

pp. 390-398 ◽

Cited By ~ 3

Author(s):

P.-J. Wan ◽

L. Yang ◽

S.-Y. Yuan ◽

Y.-H. Tang ◽

Q. Fu ◽

...

Keyword(s):

Brown Planthopper ◽

Transcript Level ◽

Nilaparvata Lugens ◽

The Body ◽

Double Stranded Rna ◽

Saccharopine Dehydrogenase ◽

Body Weights ◽

Nilaparvata Lugens Stål ◽

Phloem Feeding ◽

Wing Deformation

AbstractThe brown planthopper Nilaparvata lugens is a serious phloem-feeding pest of rice in China. The current study focuses on a saccharopine dehydrogenase (SDH) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (Lys), which plays a role in insect growth and carnitine production (as a substrate). The protein, provisionally designated as NlylsSDH [a SDH derived from yeast-like symbiont (YLS) in N. lugens], had a higher transcript level in abdomens, compared with heads, wings, legs and thoraces, which agrees with YLS distribution in N. lugens. Ingestion of Nlylssdh targeted double-stranded RNA (dsNlylssdh) for 5, 10 and 15 days decreased the mRNA abundance in the hoppers by 47, 70 and 31%, respectively, comparing with those ingesting normal or dsegfp diets. Nlylssdh knockdown slightly decreased the body weights, significantly delayed the development of females, and killed approximately 30% of the nymphs. Moreover, some surviving adults showed two apparent phenotypic defects: wing deformation and nymphal cuticles remained on tips of the legs and abdomens. The brachypterours/macropterours and sex ratios (female/male) of the adults on the dsRNA diet were lowered compared with the adults on diets without dsRNA. These results suggest that Nlylssdh encodes a functional SDH protein. The adverse effect of Nlylssdh knockdown on N. lugens implies the importance of Lys in hopper development. This study provides a proof of concept example that Nlylssdh could serve as a possible dsRNA-based pesticide for planthopper control.

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