scholarly journals ISOLATION AND IDENTIFICATION OF CHROMOBACTERIUM SP. FROM DIFFERENT ECOSYSTEMS

Author(s):  
Muthusamy Palaniswamy ◽  
Vishnu Ts

ABSTRACTObjectives: The study was aimed at isolating and identifying violacein-producing bacteria from the different ecosystems.Methods: The violet color pigment (violacein) producing bacteria was isolated and identified morphologically and genomically.Results: A total of ten bacterial Chromobacterium sp. were isolated from different sources such as soil and water. The violacein productivity wasscreened by the total isolates and the selected two strains were identified as Chromobacterium violaceum CV4 and Chromobacterium vaccinii CV5 bymorphologically and genomically.Conclusion: In the present study, the two isolate was morphologically and genomically identified as Chromobacterium violaceum CV4 andChromobacterium vaccinii CV5, last one was found to be the novel strain from natural sources which possess a high violacein producing capability.Keywords: Chromobacterium sp., Violacein, Pigment production.

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Esraa M. O. A. Ismail ◽  
Shaza W. Shantier ◽  
Mona S. Mohammed ◽  
Hassan H. Musa ◽  
Wadah Osman ◽  
...  

The recent outbreak of the highly contagious coronavirus disease 2019 (COVID-19) caused by the novel coronavirus SARS-CoV-2 has created a global health crisis with socioeconomic impacts. Although, recently, vaccines have been approved for the prevention of COVID-19, there is still an urgent need for the discovery of more efficacious and safer drugs especially from natural sources. In this study, a number of quinoline and quinazoline alkaloids with antiviral and/or antimalarial activity were virtually screened against three potential targets for the development of drugs against COVID-19. Among seventy-one tested compounds, twenty-three were selected for molecular docking based on their pharmacokinetic and toxicity profiles. The results identified a number of potential inhibitors. Three of them, namely, norquinadoline A, deoxytryptoquivaline, and deoxynortryptoquivaline, showed strong binding to the three targets, SARS-CoV-2 main protease, spike glycoprotein, and human angiotensin-converting enzyme 2. These alkaloids therefore have promise for being further investigated as possible multitarget drugs against COVID-19.


Author(s):  
Oles Fedoruk

The paper analyzes different sources of anthroponyms in the original and final texts of P. Kulish’s novel “Chorna Rada: Khronika 1663 Roku” (“The Black Council: A Chronicle of the Year 1663”). Three types of sources have been identified: the historical prototypes, names and surnames of Kulish’s friends, and archival (documentary) records. In addition, numerous notes in the early editions of the Russian novel contain references to the works of various people (M. Markevych, D. Bantysh-Kamenskyi, V. Kokhovskyi, etc.). The last group of anthroponyms stands outside of the plot, and the paper does not focus on it. The historical and autobiographical sources of anthroponyms are generally known. Among the first are prototypes of two hetmans — Yakym Somko and Ivan Briukhovetskyi, military secretary M. Vukhaievych, regimental osaul M. Hvyntovka. The second group comprises the occasional characters Hordii Kostomara (a historian M. Kostomarov), Ivan Yusko (a teacher I. Yuskevych-Kraskovskyi), Hulak (M. Hulak, the founder of The Brotherhood of Saints Cyril and Methodius), Bilozerets (Kulish’s brother-in-law V. Bilozerskyi), Petro Serdiuk (Kulish’s close friend Petro Serdiukov), Oleksa Senchylo (teacher Oleksa Senchylo-Stefanovskyi). In the novel, Kulish drew the love line as a projection of his relationship with Oleksandra Bilozerska and her mother Motrona. The characters of Petro Shramenko, Lesia Cherevanivna and her mother Melaniia have an autobiographical basis. Accordingly, Lesia’s name was also taken from real life. The third group of sources supplying the anthroponyms is archival records. The paper analуzes Kulish’s extracts from the roster of Cossack regiments of the Hetmanate (1741). This source wasn’t used previously. It contains the anthroponyms Vasyl Nevolnyk (‘Slave’), Puhach, Petro Serdiuk, Taranukha, Chepurnyi, Cherevan, Tur, Shramko and Shramchenko, Shkoda, which the author used in various editions of the novel.


2011 ◽  
Vol 5 (3) ◽  
pp. 56-65
Author(s):  
Samira Y. Yousif ◽  
Rasha Abid Ali Al-Khalidi

Total of 507 samples (clinical, environmental, food) were collected from different hospitals in Baghdad, water, soil, and different food stuffs. Biochemical and morphological characterization tests showed that seventeen isolates were identified as Aeromonas spp.These were farther characterized as Aeromonas hydrophila 10 isolates, Aeromonas sobria 2 isolates, Aeromonas eucrenophila 3 isolates, one isolate belongs to Aeromonas caviae and another one belongs to Aeromonas schubertii. Antibiotic susceptibility tests of all the isolates towards fifteen antibiotics agents were carried out and results showed that all isolates 100% were resistant to penicillin, ampicillin, ampiclox, 99% were resistant to lincomycin, 76.7% to cephalothin, 52.9% to cefotaxime. All isolates except one isolate of Aeromonas eucrenophila were sensitive to meropenem.


2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Giovanni Marasco ◽  
Marcello Maida ◽  
Gaetano Cristian Morreale ◽  
Massimo Licata ◽  
Matteo Renzulli ◽  
...  

The novel coronavirus disease 2019 (COVID-19) has been reported to affect the gastrointestinal system with a variety of symptoms, including bleeding. The prevalence of bleeding in these patients remains unclear. The aim of this meta-analysis is to estimate the rate of gastrointestinal bleeding in COVID-19 patients and its association with mortality. MEDLINE and Embase were searched through December 20, 2020. Studies reporting COVID-19 patients with and without gastrointestinal bleeding were included. Estimated prevalence with 95% confidence intervals (CI) was pooled; heterogeneity was expressed as I2. Metaregression analysis was performed to assess the impact of confounding covariates. Ten studies met the inclusion criteria and were included in the analysis. A total of 91887 COVID-19 patients were considered, of whom 534 reported gastrointestinal bleeding (0.6%) [409 (76.6%) upper and 121 (22.7%) lower gastrointestinal bleeding (UGIB and LGIB, resp.)]. The overall pooled gastrointestinal bleeding rate was 5% [95% CI 2–8], with high heterogeneity (I2 99.2%); “small study effect” was observed using the Egger test ( p = 0.049 ). After removing two outlier studies, the pooled bleeding rate was 2% [95% CI 0–4], with high heterogeneity (I2 99.2%), and no “small study effect” ( p = 0.257 ). The pooled UGIB rate was 1% (95% CI 0–3, I2 98.6%, p = 0.214 ), whereas the pooled LGIB rate was 1% (95% CI 0–2, I2 64.7%, p = 0.919 ). Metaregression analysis showed that overall estimates on gastrointestinal bleeding were affected by studies reporting different sources of bleeding. No significant association between gastrointestinal bleeding and mortality was found. In this meta-analysis of published studies, individuals with COVID-19 were found to be at risk for gastrointestinal bleeding, especially upper gastrointestinal bleeding.


2006 ◽  
Vol 42 (2) ◽  
pp. 154-159 ◽  
Author(s):  
Patricia A. Crosse ◽  
Karen Soares ◽  
Jason L. Wheeler ◽  
Kirsten L. Cooke ◽  
Chris A. Adin ◽  
...  

Chromobacterium violaceum is a saprophyte of soil and water in tropical and subtropical environments that is associated with rare but highly fatal infections in animals and humans. Systemic infection was diagnosed in two critically ill dogs from Florida. Fever was absent in both dogs. Both dogs were treated surgically and provided with intensive care, but only one survived. The identification of characteristic, violet-pigmented bacterial colonies on routine microbial cultures should alert microbiologists and clinicians to the likelihood of this dangerous pathogen. Because of the rapidly progressive nature of this infection, empirical antibiotic administration with fluoroquinolones should be employed pending susceptibility testing.


1960 ◽  
Vol 6 (2) ◽  
pp. 153-163 ◽  
Author(s):  
William A. Corpe

A method for the isolation and purification of the voluminous extracellular polysaccharide of Chromobacterium violaceum is presented. The purified product was fibrous when wet but dried into a tough, pliable film which was completely water soluble, forming a highly viscous solution. Hydrolysis of the polysaccharide, isolation and identification of the components established the presence of glucose as the principal sugar. A uronic acid and an amino sugar not conclusively identified were also present. Glucose, uronic acid, and the amino sugar were found in an approximate 5:1:1 ratio.


2020 ◽  
Vol 4 (2) ◽  
pp. 055-059
Author(s):  
Abd El-Aziz Mahmoud Hamdy ◽  
Khalil Mohamed Salah

For many years, chemical pesticides have been performed to control different pests and diseases and this may be due to their broad spectrum of action, easy of application and the relatively low cost. But these chemicals have environmental risks, thus alternative control agents are needed. Chitosan is one of the novel suggested solutions to reduce the economic losses associated with chemical pesticides. Chitosan is naturally-occurring compound, as well as safe and biodegradable which obtained from certain natural sources. Chitosan have unique properties which help to control viruses, bacteria, fungi, insects, plant nematodes and other pests locally and systemically.


Author(s):  
F. Javier MARTÍNEZ-CORTIJO ◽  
Fernando SENDRA ◽  
Juan MARCO

This study wants to establish the effects of urban waste water irrigation on the cultivation of rice for heavy metal contamination: in particular, copper (Cu), iron (Fe) and zinc (Zn). The research studies the evolution along an irrigation line, so take nine plots along it, and the levels of these elements is checked, in root, stem and grain. It is compared with other irrigation waters from different sources, with the parallel study, which analyzed other soil and water in order to determine the interaction soil-plant-water. Finally, under the conditions of the experiment, basic soils and Mediterranean conditions do not pass the relevant quantities that could endanger the consumer.


Sign in / Sign up

Export Citation Format

Share Document