IDENTIFICATION OF BIOACTIVE COMPOUNDS BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF SYZYGIUM JAMBOS (L.) COLLECTED FROM WESTERN GHATS  REGION COIMBATORE, TAMIL NADU

ABSTRACTObjective: The aim of this study was to investigate the presence of bioactive compounds in the methanolic leaf extract of Syzygium jambos.Methods: Collected leaves were shade dried and made into fine powder, extracted with methanol, and the methanolic extract was prepared andanalyzed for the presence of bioactive compounds by gas chromatography-mass spectrometry (GC-MS). The mass spectrum of the chromatographywas matched with NIST and WILEY Libraries.Results: The GC-MS analysis revealed the presence of 45 active compounds in the extract. From the GC-MS investigation, 1-Deoxy-d-mannitol3-methyl-2-methylsulfanyl-5-nitro-6-pyridin-4-ylpyrimidin-4-one, 3-Pentadecylphenol, 2-biphenylene carboxylic acid, Quinoline-3-carboxylic acid,and Stigmast-5-en-3-ol are important phytoconstituents which have antipyretic and antiparasitic activities.Conclusion: The present investigation revealed preliminary information on phytocompounds presented in S. jambos leaf extract which is very usefulfor the human community.Keywords: Syzygium jambos, Gas chromatography-mass spectrometry analysis, 1-Deoxy-d-mannitol, Phytoconstituents, Methanolic leaf extract.

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GAS CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS OF METHANOL EXTRACT OF ZANTHOXYLUM RHETSA DC. FRUITS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i12.26821 ◽

2018 ◽

Vol 11 (12) ◽

pp. 247

Author(s):

Kuladip Gurav ◽

Varsha Jadhav (rathod)

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Bioactive Compounds ◽

Biological Activities ◽

Acid Methyl Ester ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Hexadecanoic Acid ◽

Spectrometry Analysis ◽

Chromatography Mass Spectrometry

Objective: The aim was to investigate important bioactive compounds, biological activities, and medicinal importance of Zanthoxylum rhetsa fruits.Methods: The present work was carried out by gas chromatography–mass spectrometry (GC-MS) method for the identification of different compounds.Result: The methanolic extract of fruits showed 32 chemical compounds which are identified through GC-MS analysis. Among them, some of the compound names and percentage values are as follows: 2-propanone, 1,3-dihydroxy (48.9%), 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl (33.7%), 2-furancarboxaldehyde, 5-[hydroxymethyl] (50.2%), 1-Heptatriacontanol (34.4%), 9,12-octadecadienoic acid (zz)- (48.6%), cholestan- 3-ol,2-methylene, [3β,5α] (75.0%), 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (90.8), 2-furancarboxaldehyde, 5-[hydroxymethyl] (92.0%), hydroquinone (64.9%), n-hexadecanoic acid (37.5%), octadecanoic acid (28.2%), 9,12,15, octadecatrienoic acid, 2-[(trimethylsiyl]oxy]-1- [(trimethylsiyl]oxy]methyl] ethyl ester, [zzz] (22.6%), 9-hexadecanoic acid (10.3%), digitoxin (18.8%), 8,11,14-eicosatrienoic acid methyl ester, [zzz] (25.5%), and oleic acid (16.5%). Most of the identified compounds in the crude methanolic extracts exhibit some bioactivities, namely anticancer, anti-inflammatory, antimicrobial, hepatoprotective, antioxidant, hypocholesterolemic, nematicide, pesticide, anti-androgenic flavor, hemolytic, 5-alpha-reductase inhibitor, insectifuge, antiarthritic, anti-coronary, cardiovascular, anti-breast cancer, aromatic, and insectifuge. On the basis of the above investigation, the fruits can be recommended as a treasure of bioactive compounds and it plays a promising role in herbal medicine.Conclusion: The present study reveals that fruits of Z. rhetsa contain various bioactive compounds. Digitoxin is recorded in the ripened fruit of Z. rhetsa and it shows the anticancerous and cardiac arrest properties. Hence, in future, this plant will play a promising role in curing cancer.

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IDENTIFICATION OF BIOACTIVE COMPOUNDS IN FLOWER OF TABERNAEMONTANA DIVARICATA (L.) USING GAS CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i9.34559 ◽

2019 ◽

pp. 129-132

Author(s):

KALAIMAGAL C

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Bioactive Compounds ◽

Ethanolic Extract ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Spectrometry Analysis ◽

Tabernaemontana Divaricata ◽

Chromatography Mass Spectrometry ◽

Ability To Act

Objective: Herbs are a key resource with therapeutic properties. Nowadays, there is a focus on the identification of bioactive compounds with the ability to act against various disorders. Methods: In the present study, gas chromatography–mass spectrometry analysis was conducted to determine the occurrence of different phytochemical compounds in ethanolic flower extract of Tabernaemontana divaricata (L.). Results: The ethanol extract of flower revealed the presence of several bioactive compounds such as n-hexadecanoic acid, squalene, Vitamin D3, Vitamin A aldehyde, desulfosinigrin, and Urs-12-en-24-oic acid, 3-oxo-, methyl ester, (+)-. Conclusion: The perceived compounds from ethanolic extract of flower have diverse beneficial properties such as antimicrobial, antioxidant, cancer anticipatory effect, pesticide, and antiarthritic.

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GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF METHANOLIC LEAF EXTRACT OF CASSIA ANGUSTIFOLIA VAHL.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9s3.14512 ◽

2016 ◽

Vol 9 (9) ◽

pp. 111 ◽

Cited By ~ 4

Author(s):

Shahina Parveen ◽

Anwar Shahzad ◽

Anamica Upadhyay ◽

Vikas Yadav

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Vitamin E ◽

Leaf Extract ◽

Methanolic Extract ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Spectrometry Analysis ◽

Cassia Angustifolia ◽

Chromatography Mass Spectrometry

ABSTRACTObjective: The leaves of Cassia angustifolia Vahl. are employed for the treatment of several human diseases. Therefore, the present study wasundertaken to determine the phytocomponents present in the methanolic extract of the leaves by gas chromatography-mass spectrometry (GC-MS)Methods: The collected leaf samples were dried and extracted in methanol. Screening of the extract was done by GC-MS which is an importanttechnique for the separation and identification of different phytochemicals.Results: The methanolic extract of the leaves of C. angustifolia revealed the presence of 45 different phytochemicals. The prevailing compounds were1 butanol, 3 methyl acetate (area % 7.47), 6, 6-dideutero-nonen-1-Ol-3 (area % 10.45), pentadecanoic acid (area % 9.22), and squalene (area %12.30). Vitamin E (area % 3.85) has also been found in the leaf extract. Some of the compounds possess biological activities.Conclusions: It can be concluded from the present study that some of the identified phytochemicals could be responsible for the medicinal value orbiological activity of the plant leaves.Keywords: Senna, Leaf extract, Methanol, Gas chromatography-mass spectrometry, Phytochemicals, Vitamin E.

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