INHIBITION OF CARSINOGENESIS BY SEED AND SOYBEAN MEAL EXTRACT IN COLON OF MICE: APOPTOSIS AND DYSPLASIA
Objective: Colon cancer is a major public health problem. Soybean has demonstrated chemopreventive and anti-cancer. Here, we have investigated the effect of a standardized seed and soybean meal extract with content of lunasin, here named GE and SE. They are botanical drug substance in experimental models of colon cancer in vivo.Methods:The effect of GE and SEwere examined onthe preneoplastic lesions (aberrant crypt foci), polyps and tumours induced by the carcinogenic agentazoxymethane (AOM) (10 mg/kg) and dextran sodium sulfate (DSS) 2% as well as in a xenograft model of colon cancer in mice.Results:.GE and SE increased apoptosis (p = 0,001). GE (150 mg/kg) has the highest impact level of apoptosis (p = 0,009). GE and SE decreased dysplasia (p = 0,024). GE (200 mg/kg) has the highest impact level of dysplasia (p = 0,002) and SE (200 mg/kg) has the second impact level of dysplasia (p = 0,003).Conclusions: GE and SE inhibition of colon carsinogenesis with incrase level of apoptosis and decrease level of dysplasiaKeyword: soybean, lunasin, colon cancer, azoxymethane, dextran sodium sulfate, apoptosis, dysplasia