scholarly journals EVALUATION OF IN VITRO IMMUNOMODULATORY ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACT OF EULOPHIA NUDA LINDL

2018 ◽  
Vol 11 (11) ◽  
pp. 352 ◽  
Author(s):  
Vanita Kanase ◽  
Diptesh T Patil
Keyword(s):  
Lysosomal Enzyme ◽  
Stimulation Index ◽  
Ethanolic Extract ◽  
In Vivo Studies ◽  
Phagocytic Index ◽  
Immunomodulatory Activity ◽  
Myeloperoxidase Activity ◽  
Enzyme Release

Objective: The aim of this study was to evaluate the in vitro immunomodulatory activity of aqueous and ethanolic extract of dried tubers of Eulophia nuda.Methods: Effect of both the extracts was evaluated at various concentrations (832–6.5 μg/ml) for secretion of mediators such as nitric oxide (NO), superoxide, lysosomal enzyme, and myeloperoxidase activity of isolated murine peritoneal macrophages.Results: The extracts showed stimulation of NO, statistically significant at 832 μg/ml (SI 1.739) for ENA and at 832 μg/ml (stimulation index [SI] 1.662) for ENE; significant stimulation on lysosomal enzyme release for ENA at 832 μg/ml (SI 1.404) and ENE at 832 μg/ml (SI 1.513); myeloperoxidase activity was statistically significant for ENA at 832 μg/ml (SI 1.728) and ENE at 832 μg/ml (SI 1.770).Conclusion: In vitro phagocytic index showed significant results and thus proving the need for confirmation through in vivo studies.

Stobadine inhibits lysosomal enzyme release in vivo and in vitro

Life Sciences ◽  
1999 ◽  
Vol 65 (18-19) ◽  
pp. 1905-1907 ◽  
Author(s):  
Jana Navarová ◽  
Tatiana Mačičková ◽  
Katarina Horáková ◽  
Miroslava Urbančíková
Keyword(s):  
Lysosomal Enzyme ◽  
Enzyme Release

HERBAL CREAMS OF REISHI EXTRACT AND TEA TREE OIL FOR HIRSUTISM–AN IN VIVO STUDY

2020 ◽  
pp. 106-110
Author(s):  
SANGEETA CHOUDHURY ◽  
BLR MADHAVI
Keyword(s):  
Topical Application ◽  
Hair Growth ◽  
Ethanolic Extract ◽  
Hair Follicles ◽  
In Vivo Studies ◽  
Control Group ◽  
Tea Tree Oil ◽  
Tea Tree

Objective: The aim of this work to formulate, evaluate and compare the effectiveness of herbal creams containing extract of reishi and tea tree oil for treating hirsutism. Methods: Herbal ingredients were authenticated. Cream base was initially formulated. Three formulations of herbal cream were prepared. Reishi ethanolic extract, tea tree oil, and combination of tea tree oil and reishi extract were added to the cream base and formulated cream were named as RHC, THC and RTC respectively. In vitro evaluations on herbal creams were done for the physicochemical characteristics. In vivo studies were carried out on female Swiss Albino mice for the activity against hair growth by topical application of cream to shaved skin. The histological and morphometric evaluation was carried out. Skin irritancy study was conducted. Results: The herbal creams showed desirable physicochemical properties like pH, viscosity and spreadability. Statistical analysis for the length of hair was performed by using one way ANOVA followed by DUNNET’S post hoc test where THC and RTC were found to be significant whereas RHC showed no significant reduction of hair growth compared to control. RTC showed a significant effect at p<0.05 and hair growth reduction was significant for THC at p<0.001 compared to the control group. RTC and THC showed mild to moderate reduction in the size of the hair follicles with a reduction of sebaceous gland size in the histological analysis. Conclusion: Topical application of herbal creams to mice showed that hair growth was fastest in group RHC and was slowest in group THC and intermediate with RTC. It can be concluded that these herbal actives can be used as an effective treatment against hirsutism. Within the study period, tea tree oil was found to be more effective than reishi extract and the combination product. Further formulation studies and in vivo studies need to be carried out on reishi to assess its effectiveness against hirsutism.

Production of Peptides Inducing Chemotaxis and Lysosomal Enzyme Release in Human Neutrophils by Intestinal Bacteria in Vitro and in Vivo

1988 ◽  
Vol 23 (1) ◽  
pp. 121-128 ◽  
Author(s):  
V. S. Chadwick ◽  
D. M. Mellor ◽  
D. B. Myers ◽  
A. C. Selden ◽  
A. Keshavarzian ◽  
...  
Keyword(s):  
Lysosomal Enzyme ◽  
Intestinal Bacteria ◽  
Human Neutrophils ◽  
Enzyme Release

scholarly journals In Silico, In Vitro, and In Vivo Antitumor and Anti-Inflammatory Evaluation of a Standardized Alkaloid-Enriched Fraction Obtained from Boehmeria caudata Sw. Aerial Parts

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 4018
Author(s):  
Paula P. de Paiva ◽  
Julia H. B. Nunes ◽  
Fabiana R. Nonato ◽  
Ana L. T. G. Ruiz ◽  
Rafael R. T. Zafred ◽  
...  
Keyword(s):  
Human Tumor ◽  
Antitumor Agent ◽  
Docking Studies ◽  
In Vivo Studies ◽  
Myeloperoxidase Activity ◽  
Aerial Parts ◽  
M Phase ◽  
Anti Inflammatory

In the context of the cancer-inflammation relationship and the use of natural products as potential antitumor and anti-inflammatory agents, the alkaloid-enriched fraction of Boehmeriacaudata (BcAEF) aerial parts was evaluated. In vitro antiproliferative studies with human tumor cell lines showed high activity at low concentrations. Further investigation on NCI-H460 cells showed an irreversible effect on cell proliferation, with cell cycle arrest at G2/M phase and programmed cell death induction. Molecular docking studies of four alkaloids identified in BcAEF with colchicine’s binding site on β-tubulin were performed, suggesting (−)-C (15R)-hydroxycryptopleurine as the main inductor of the observed mitotic death. In vivo studies showed that BcAEF was able to reduce Ehrlich tumor volume progression by 30 to 40%. Checking myeloperoxidase activity, BcAEF reduced neutrophils migration towards the tumor. The in vivo anti-inflammatory activity was evaluated by chemically induced edema models. In croton oil-induced ear edema and carrageenan (CG)-induced paw edema models, BcAEF reduced edema around 70 to 80% together with inhibition of activation and/or migration of neutrophils to the inflammatory area. All together the results presented herein show BcAEF as a potent antitumor agent combining antiproliferative and anti-inflammatory properties, which could be further explored in (pre)clinical studies.

scholarly journals Study of Antioxidant Activity of Stalk and Fruit of Solanum melongena L. (Solanaceae)

2019 ◽  
pp. 1-7
Author(s):  
Kady Diatta ◽  
William Diatta ◽  
Alioune Dior Fall ◽  
Serigne Ibra Mbacké Dieng ◽  
Amadou Ibrahima Mbaye ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Solanum Melongena ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
In Vivo Studies ◽  
Ethanol Extracts

Background : Nowadays with the appearance of diseases such as cancer, atherosclerosis, free radicals are often singled out. What motivates scientific research in natural antioxidants. Aim/Objective : The aim of this study was to determine the antioxidant activity of the stalks and the fruit of Solanum melongena L. Study Duration : The period of the study was done on 25th July, 2015 at the Department of Pharmacy, Faculty of Medecine, Pharmacy and Odontology, University of Dakar, Senegal. Methodology : Antioxidant activity was evaluated through two methods (DPPH and FRAP). Results : For the FRAP test, at the highest concentration (83.3 µg/ml) the aqueous extract of the fruit (0.90±0.08) has a higher reducing power compared to those of ethanol extracts from the fruit (0.77±0.41) and the stalk (0.85±0.004). These results remain inferior to that of tannic acid (0.95± 0.0005). The DPPH test reveals that the ethanolic extract of the fruit is more effective in reducing the free radical DPPH with an inhibitory concentration 50 (IC 50) equal to 3.37±0.03 μg / ml, followed by the ethanolic extract of the stalks (IC 50 = 4.46±0.24 μg / ml) and finally the aqueous extract of the fruit (IC50 = 9.6±0.026 μg / ml). Conclusion : These results make it possible to confirm the in vitro activity of the parts studied, but in vivo studies are necessary in order to know the acute and chronic toxicities. Finally, perform a bio-guided fractionation to determine the molecules responsible for the antioxidant activity.

scholarly journals In Vitro and In Vivo Leishmanicidal Activity ofAstronium fraxinifolium(Schott) andPlectranthus amboinicus(Lour.) Spreng againstLeishmania (Viannia) braziliensis

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Silvio César Gomes de Lima ◽  
Maria Jania Teixeira ◽  
José Evaldo Gonçalves Lopes Júnior ◽  
Selene Maia de Morais ◽  
Alba Fabiola Torres ◽  
...  
Keyword(s):  
Standard Treatment ◽  
Ethanolic Extract ◽  
Parasite Growth ◽  
In Vivo Studies ◽  
In Vitro Tests ◽  
Intralesional Injections ◽  
Plectranthus Amboinicus ◽  
Ethanolic Leaf Extract

The aim of the present work was to evaluate antileishmanial activity ofAstronium fraxinifoliumandPlectranthus amboinicus. For the in vitro tests, essential oil ofP. amboinicus(OEPA) and ethanolic extracts fromA. fraxinifolium(EEAF) were incubated with 106  promastigotes ofL. (Viannia) braziliensis. The OEPA was able to reduce the parasite growth after 48 h; nonetheless, all the EEAFs could totally abolish the parasite growth. For the in vivo studies, BALB/c mice were infected subcutaneously (s.c.) with 107  L. braziliensispromastigotes. Treatment was done by administering OEPA intralesionally (i.l.) for 14 days. No difference was found in lesion thickness when those animals were compared with the untreated animals. Further, golden hamsters were infected s.c. with 106  L. braziliensispromastigotes. The first protocol of treatment consisted of ethanolic leaf extract fromA. fraxinifolium(ELEAF) administered i.l. for 4 days and a booster dose at the 7th day. The animals showed a significant reduction of lesion thickness in the 6th week, but it was not comparable to the animals treated with Glucantime. The second protocol consisted of 15 daily intralesional injections. The profiles of lesion thickness were similar to the standard treatment. In conclusion, in vivo studies showed a high efficacy when the infected animals were intralesionally treated with leaf ethanolic extract fromA. fraxinifolium.

scholarly journals EVALUATION OF IN VIVO IMMUNOMODULATORY ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACT OF CAPPARIS MOONII

2020 ◽  
pp. 63-66
Author(s):  
VANITA G KANASE ◽  
SHRUTI SHETTIGAR
Keyword(s):  
Antibody Titer ◽  
Ethanolic Extract ◽  
Experimental Methods ◽  
Delayed Type Hypersensitivity ◽  
Phagocytic Index ◽  
Immunomodulatory Activity ◽  
Dried Fruits ◽  
Carbon Clearance ◽  
Stimulation Of

Objective: The present study was intended to evaluate the in vivo immunomodulatory activity of aqueous and ethanolic extract of dried fruits of Capparis moonii Wight. Methods: The effect of both the extracts was evaluated by carbon clearance assay in mice, and they showed a significant increase in the phagocytic index at 50, 100, and 200 mg/kg dose. The effect of the extracts on delayed-type hypersensitivity (DTH) and antibody titer assay was evaluated in ovalbumin immunized mice. Results: Both extracts showed a significant effect on DTH response and stimulation of antibody titer at 200 mg/kg dose. The effects of the extracts in cyclophosphamide-induced myelosuppression mice were also statistically significant. Conclusion: The studies demonstrated specific and non-specific immunostimulating properties of both the ethanol and aqueous extract of C. moonii fruits in various in vivo experimental methods suggesting its therapeutic usefulness in immunocompromised conditions.

Antiinflammatory and immunomodulatory activity of an ethanolic extract from the stem bark of Terminalia catappa L. (Combretaceae): In vitro and in vivo evidences

2016 ◽  
Vol 192 ◽  
pp. 309-319 ◽  
Author(s):  
Oyindamola O. Abiodun ◽  
Alba Rodríguez-Nogales ◽  
Francesca Algieri ◽  
Ana Maria Gomez-Caravaca ◽  
Antonio Segura-Carretero ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Stem Bark ◽  
Immunomodulatory Activity ◽  
Terminalia Catappa

scholarly journals PHYTOCHEMICAL SCREENING AND ANTIDIABETIC, ANTIHYPERLIPIDEMIC PROPERTIES OF LEAVES OF Filicium decipiens IN STREPTOZOTOCIN INDUCED DIABETIC RATS.

2019 ◽  
Vol 9 (5) ◽  
pp. 62-66
Author(s):  
Elias Akila ◽  
C Geetha Priya
Keyword(s):  
Ethanolic Extract ◽  
Soxhlet Extraction ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
In Vivo Studies ◽  
Phytochemical Screening ◽  
Pharmacological Screening ◽  
Selection Of

The Present study was undertaken with a view to evaluate the Phytochemical & Pharmacological activities of the leaves of Filicium decipiens (Sapindaceae).  The shade dried powdered plant material was subjected to successive soxhlet extraction with Petroleum Ether, Chloroform, Ethyl acetate and Ethanol. The various extracts were then subjected to preliminary phytochemical screening which revealed the presence of flavanoids, saponins, tannins, phenols, sugars, lipids, alkaloids & steroids. Selection of active extract for in vivo studies was based on preliminary phytochemical tests and in vitro glucose diffusion inhibition potential and accordingly ethanolic and chloroform extracts were chosen for further studies. Pharmacological screening included evaluation of antidiabetic and hypolipidemic activity of chloroform and ethanolic extracts (200mg/kg b.w) on Streptozotocin induced diabetic rats. The ethanolic extract was found to be more effective and brought about significant antidiabetic & hypolipidemic potential. This was due to the presence of one or more phytoconstituents present in ethanol extract. Thus, the present study validates the traditional claim of the plant and endorses a scientific proof in future.

scholarly journals Immunomodulation Effect of Kaempferia galanga Ethanolic Extract On In Vitro Lymphocyte Cell Proliferation

2021 ◽  
Vol 32 (1) ◽  
pp. 31
Author(s):  
Dianita Dwi Sugiartanti ◽  
Ening Wiedosari
Keyword(s):  
Cell Proliferation ◽  
Ethanolic Extract ◽  
Immunomodulatory Activity ◽  
Splenocyte Proliferation ◽  
Treatment Groups ◽  
Kaempferia Galanga ◽  
Cell Proliferation Activity ◽  
Lymphocyte Cell

<p><em>Kaempferia galanga</em> L. is a traditional medicine with antitumor properties, as indicated by its immunomodulatory activities. This study aimed to determine the effect of <em>K. galanga</em> on lymphocyte cell proliferation activity as an indicator of immunomodulatory properties. This study was conducted at the Indonesian Research Center for Veterinary Science (February to April 2018). The immunomodulatory activity of the extract was evaluated with an <em>in vitro</em> splenocyte proliferation assay. The assay was based on cellular enzymatic synthesis to transform the XTT from formazan tetrazolium as an indicator. The <em>K. galanga</em> extract was obtained by 96% ethanol extraction. The test was conducted in an aseptic condition, consisted of five treatment groups with three replications each.  Three groups of splenocyte cell culture, each with extract concentration of 2.5 µg.ml<sup>-1</sup>, 25 µg.ml<sup>-1</sup>, and 250 µg.ml<sup>-1</sup>, as well as a positive (Concanavalin A/Con A) and negative (cell only) control. The cell suspension (10x10<sup>4 </sup>cells/ml) was distributed on 96-well plates and cultured following the treatment groups. The same five plates were made for five days of observation and retrieved daily by observing an Elisa reader at 450 nm. The extract of <em>K. galanga </em>at 2.5 µg.ml<sup>-1</sup>, 25 µg.ml<sup>-1</sup>, and 250 µg.ml<sup>-1</sup> significantly (P &lt;0.05) promoted splenocyte proliferation compared to control.  Therefore, it was expected that <em>K. galanga </em>has a high potential to be used as immunomodulators. Hence, further investigations should be done to clarify the mechanisms of the immunomodulatory effect of <em>K. galanga</em> as an antitumor <em>in vivo</em>.</p>

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