scholarly journals PHYTOCHEMICAL EVALUATION OF TILIACORA RACEMOSA COLEBR. USING GAS CHROMATOGRAPHY - MASS SPECTROMETRY (GC-MS)

2018 ◽  
Vol 11 (2) ◽  
pp. 350
Author(s):  
Yogeshwari C ◽  
Kumudha P
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Quinic Acid ◽  
Physicochemical Analysis ◽  
Drug Formulation ◽  
Gas Chromatography Mass Spectrometry ◽  
Isolation And Characterization ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

 Objective:The objective of this study is to characterize the phytoconstituents of Tiliacora racemosa Colebr. using gas chromatography mass spectrometry (GC-MS).Methods: Preliminary phytochemical and physicochemical analysis was carried out using standard procedures. GC-MS analysis of methanolic extract was carried out using Thermo GC-Trace Ultra version: 5.0, Thermo MS DSQ with a DB 35MS capillary standard non-polar column and gas chromatograph interfaced to a mass selective detector (MS DSQ II) with Xcalibur software.Results: Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, phenols, tannins, triterpenoids, steroids, proteins and amino acids, carbohydrates, saponins and coumarin. Quinones, anthraquinones, glycosides and fixed oil were absent. GC-MS analysis revealed the presence of 28 compounds of which quinic acid (retention times [RT]: 15.65) and inositol, 1-deoxy-(CAS) (RT: 19.24) was observed as abundant compounds.Conclusion: The presence of various bioactive compounds confirms the medicinal importance and it’s application for curing various diseases by traditional practitioners. However, isolation and characterization of potential bioactive compounds would lead to drug formulation.

scholarly journals Phytochemical screening and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Silybum marianum (L).

2019 ◽  
Vol 9 (1) ◽  
pp. 85-89 ◽  
Author(s):  
M Padma ◽  
S Ganesan ◽  
T Jayaseelan ◽  
S Azhagumadhavan ◽  
P Sasikala ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Ethanolic Extract ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Silybum Marianum ◽  
Selective Sequential Extraction ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Objective: To investigate the phytochemicals and GC-MS analysis of ethanol extracts of Silybum marianum. Methods: The air-dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, ethanol to obtain an ethanolic extract. Then, each of the extracts was further subjected to gas chromatography-mass spectrometry. Results: Qualitative determination of the different biologically active compounds from crude extracts of Silybum marianum using gas chromatography-mass spectrometry revealed different types of high and low molecular weight chemical entities with varying amounts present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important. Conclusions: The study established the chemical composition and anticancer activity of the plant. Keywords: Silybum marianum, Phytochemicals screening, GC–MS analysis, Bioactive compounds.

scholarly journals Gas chromatography-mass spectrometry chemical profile investigation and biological activities of ethylacetate fraction of Baobab (Adansonia digitata L.) pulp used in the treatment of urinary tract infections

2022 ◽  
Vol 6 (1) ◽  
Author(s):  
Kehinde O. Fagbemi ◽  
Daniel A. Aina ◽  
Roger M. Coopoosamy ◽  
Olufunmiso O. Olajuyigbe
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Urinary Tract ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Fruit Pulp ◽  
Adansonia Digitata ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Background: As a result of high percentage of women infected with urinary tract infection (UTI) annually, many rural dwellers use Adansonia digitata fruit pulp as herbal cure. Adansonia digitata L. (Malvaceae) is one of the great trees idolised in Africa because of its therapeutic properties.Aim: This study aimed at identifying chemical compounds in the ethylacetate fraction of the baobab fruit pulp and indicated their biological activities to justify its use for the folkloric treatment of UTI.Methods: The crude extract from Baobab fruit pulp was partitioned and ethylacetate extract was used for assay. Gas chromatography-mass spectrometry (GC–MS) analysis was performed to identify the bioactive compounds in the ethylacetate fraction, antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays whilst the total phenolic and flavonoid contents were determined by already adopted protocols. The antibacterial properties were tested against some urinary tract pathogens using macro broth dilution method.Results: A total of 36 bioactive compounds were identified by GC–MS analysis, most of them have been reported as antibacterial, antioxidant, antidiabetic, antiasthma, antifungal, anti-inflammatory and anti-cancer agents. The extract exhibited highest activity against Pseudomonas aeruginosa American Type Culture Collection (ATCC) 19582 (1.22 mg/mL) whilst Escherichia coli ATCC 8739 (˃ 8.6 mg/mL) displayed high level of resistance amongst the five bacterial isolates used in this study.Conclusion: These findings indicated that the extract contained bioactive compounds of therapeutic importance with significant antioxidant and antibacterial potentials and justify the folkloric use of this fruit in the treatment of UTIs by many African dwellers.

scholarly journals ANALISIS GC-MS (GAS CHROMATOGRAPHY - MASS SPECTROMETRY) EKSTRAK METANOL DARI UMBI RUMPUT TEKI (Cyperus rotundus L.)

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 849
Author(s):  
Ellen Hotmian ◽  
Elly Suoth ◽  
Fatimawali Fatimawali ◽  
Trina Tallei
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Cyperus Rotundus ◽  
Gas Chromatography Mass Spectrometry ◽  
The Public ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

ABSTRACTThe Nut Grass Tuber (Cyperus rotundus L.) is a plant that is believed by the public to cure several diseases. According to a study conducted in vivo and in vitro, the extract the tuber root has many potentials such as anticancer, anti-inflammatory, antibacterial, etc. This study aims to determine the polar bioactive compounds contained in the bulb tubers. The method used was the extraction of nut tuber dry powder using methanol as a solvent by maceration process and then analyzed using gas chromatography - mass spectrometry (GC-MS) to obtain information on the content of the tubers The results of GC-MS analysis from this study indicate that there are 177 possible components of the compound extracted using methanol. The results also showed that there were three possible polar bioactive compounds at the highest peak of the GC-MS analysis, namely 7-Isopropenyl-1,4a-dimethyl-4,4a, 5,6, 7,8-hexahydro-3H-naphthalen-2-one, 1 (2H) -Naphthalenone, 3,4,4a, 5,6,7-hexahydro-4a, 5-dimethyl-3- (1-methylethenyl) -, [3S- (3a, 4aa, 5a)] -, and 2 (1H) Naphthalenone, 3,5,6,7,8,8a-hexahydro-4,8a-dimethyl-6- (1-methylethenyl) -.Keywords         : Methanolic extract, nut grass tuber, Cyperus rotundus L., GC-MS   ABSTRAKUmbi Rumput Teki (Cyperus rotundus L.) merupakan tumbuhan yang dipercayai masyarakat dapat menyembuhkan beberapa penyakit. Menurut sebuah penelitian yang dilakukan secara in vivo maupun in vitro, kandungan ekstrak umbi rumput teki memiliki banyak potensi seperti antikanker, antiinflamasi, antibakteri, dll. Penelitian ini bertujuan untuk mengetahui senyawa-senyawa bioaktif polar yang terkandung pada umbi rumput teki. Metode yang digunakan yaitu ekstraksi serbuk kering umbi rumput teki menggunakan pelarut metanol dengan proses maserasi kemudian dianalisis menggunakan kromatografi gas – spektrometri massa (GC-MS) untuk mendapatkan informasi kandungan dalam umbi rumput teki. Hasil analisis GC-MS dari penelitian ini menunjukkan bahwa terdapat 177 kemungkinan komponen senyawa yang diekstraksi menggunakan pelarut methanol. Hasil penelitian juga menunjukkan bahwa terdapat tiga kemungkinan senyawa bioaktif polar pada puncak tertinggi hasil analisis GC-MS, yaitu 7-Isopropenyl-1,4a-dimethyl-4,4a,5,6,7,8-hexahydro-3H-naphthalen-2-one, 1(2H)-Naphthalenone, 3,4,4a,5,6,7-hexahydro-4a,5-dimethyl-3-(1-methylethenyl)-, [3S-(3a,4aa,5a)]-, dan 2(1H)Naphthalenone, 3,5,6,7,8,8a-hexahydro-4,8a-dimethyl-6-(1-methylethenyl)-. Kata kunci      : Ekstrak metanol, umbi rumput teki, Cyperus rotundus L., GC-MS

scholarly journals PHYTOCHEMISTRY SCREENING AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF BIOACTIVE COMPOUNDS PRESENT IN KARO TRADITIONAL OIL, AN INDONESIAN TRADITIONAL HERBAL MEDICINE

2020 ◽  
pp. 204-208
Author(s):  
NOVA PRIMADINA ◽  
ACHMAD BASORI ◽  
DAVID S PERDANAKUSUMA
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Inflammatory Cytokines ◽  
Bioactive Compounds ◽  
Healing Process ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Wound Healing Process ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Objective: The purpose of this research was to observe the bioactive compounds of Karo traditional oil by phytochemistry screening and gas chromatography-mass spectrometry (GC-MS) analysis. Alkaloid, Results: Terpenoid and flavonoid compounds were found, and no polyphenol compound from phytochemistry screening and 16 constituents were identified from the GC-MS analysis, α-pinene was found as the major component (74.47%), delta-3-carene (9.62%), 1% of octane, dodecane, camphor, undecane, isodecane, sabinene, hexadecane, nonane, and <1% tridecane, cyclohexane, pentacosane, heptadecan, limonene, and camphene. Conclusion: Karo traditional oil works as a potent anti-inflammation at the inflammation phase of the wound healing process by suppressing the pro-inflammatory cytokines and promote anti-inflammatory cytokines.

scholarly journals SCREENING OF BIOACTIVE COMPOUNDS FROM UNKNOWN MARINE SPONGES COLLECTED FROM KOVALAM, CHENNAI

2017 ◽  
Vol 10 (5) ◽  
pp. 231 ◽  
Author(s):  
Yuvarani Thambidurai ◽  
Skm Habeeb ◽  
Joe Kizhakudan ◽  
Sudarsanam D
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Chemical Compounds ◽  
Marine Sponges ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Dysidea Herbacea

Objective: This study is designed to hunt for the presence of bioactive compounds from three marine sponges collected from Kovalam.Methods: Zoochemical analysis is performed to screen for the presence of secondary metabolites. Based on those results, only two sponges which showed a significant presence of secondary metabolites has been subjected to gas chromatography-mass spectrometry (GC-MS) analysis to identify the unknown chemical compounds present in those sponges.Results: On analyzing the results, two sponges, namely, Dysidea herbacea and Sigmadocia pumila, has shown a significant presence of secondary metabolites while the third sponge Acanthella elongata have shown moderate presence of secondary metabolites. Since the first two sponges results are remarkable, these two samples have been subjected to GC-MS analysis to separate and identify the unknown chemical compounds present in the sample.Conclusion: Samples, namely, D. herbacea and S. pumila, indicated the presence of several components. From both the sponges, eleven different secondary metabolites were identified by GC-MS. Most of these compounds are widely used in cosmetic, pharmaceutical, and other industries and therefore a vital source for industrial biotechnology and related products in healthcare and skincare.Keywords: Dysidea herbacea, Sigmadocia pumila, Acanthella elongata, Secondary metabolites, Gas chromatography-mass spectrometry analysis, Bioactive compounds.

scholarly journals The Analysis of Bayer Liquor by SPME-GC-MS of Derivatized Organic Poisons

Proceedings ◽  
2021 ◽  
Vol 57 (1) ◽  
pp. 101
Author(s):  
Virgil Badescu ◽  
Raluca Senin
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Bayer Liquor ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Silylating Agent

The aim of this article was the gas chromatography–mass spectrometry (GC-MS) analysis oforganic matter from a residual liquor sample (S.C. Alum S.A., Tulcea), extracted by the solid-phasemicroextraction method (SPMA) and derivatized with N-(tert-butyldimethylsilyl)-Nmethyltrifluoroacetamide(MTBSTFA) as the silylating agent. [...]

scholarly journals Development and validation of the method for the detection of glimepiride via derivatization employing N-methyl-N-(trimethylsilyl) trifluoroacetamide using gas chromatography-mass spectrometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Priyanka Verma ◽  
Atul Bajaj ◽  
R. M. Tripathi ◽  
Sudhir K. Shukla ◽  
Suman Nagpal
Keyword(s):  
Diabetes Mellitus ◽  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Drug Users ◽  
Coefficient Of Determination ◽  
Gas Chromatography Mass Spectrometry ◽  
Biological Matrices ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
The Stability

Abstract Background Recent advances in the diversified anti-diabetic drugs have appeared in the startling increase in the count of poisoning cases. The epidemics of diabetes mellitus are increasing; hence, the no. of anti-diabetic drug users raised by 42.9%. The use of glimepiride raised to 24%. As the toxicity and drug cases are also escalating with increasing epidemics of diabetes mellitus, a novel gas chromatography-mass spectrometry (GC-MS) method for detecting glimepiride in biological matrices is developed. Results Liquid-liquid extraction method was employed by using 1-butanol: hexane (50:50, v/v) under an alkaline medium, and then back extraction was done via acetic acid. Distinct derivatization techniques were employed for the sample preparation for GC-MS analysis, i.e., silylation and acylation. Derivatization approaches were optimized under different parameters, i.e., reaction temperature and reaction time. N-Methyl-N-(trimethylsilyl) trifluoroacetamide [MSTFA] was found to be the best sound derivatization reagent for the GC-MS analysis of glimepiride. Total ion current (TIC) mode was selected for the monitoring of ions of trimethylsilyl (TMS) derivative of glimepiride with an m/z ratio of 256. Distinct parameters like specificity, carryover, stability, precision, and accuracy were evaluated for validating the identification method. The GC-MS method is found to be linear and illustrated within the range 500 to 2500 ng/ml with the value of R2 (coefficient of determination) at 0.9924. The stability of the extracted and derivatized glimepiride was accessed with regard to processed/extracted sample conditions and autosampler conditions, respectively. Accuracy at each concentration level was within the + 15% of the nominal concentration. Precision (%) for the interday and intraday analysis was found to be in the respectable spectrum. Conclusion Henceforth, the proposed GC-MS method can be employed for the determination of glimepiride in biological matrices.

scholarly journals IDENTIFICATION OF BIOACTIVE COMPOUNDS BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF SYZYGIUM JAMBOS (L.) COLLECTED FROM WESTERN GHATS REGION COIMBATORE, TAMIL NADU

2016 ◽  
Vol 10 (1) ◽  
pp. 364 ◽  
Author(s):  
Devakumar Devakumar Joseph ◽  
Keerthana Veerasamy ◽  
Sudha Siva Singaram
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Carboxylic Acid ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Syzygium Jambos ◽  
Chromatography Mass Spectrometry

ABSTRACTObjective: The aim of this study was to investigate the presence of bioactive compounds in the methanolic leaf extract of Syzygium jambos.Methods: Collected leaves were shade dried and made into fine powder, extracted with methanol, and the methanolic extract was prepared andanalyzed for the presence of bioactive compounds by gas chromatography-mass spectrometry (GC-MS). The mass spectrum of the chromatographywas matched with NIST and WILEY Libraries.Results: The GC-MS analysis revealed the presence of 45 active compounds in the extract. From the GC-MS investigation, 1-Deoxy-d-mannitol3-methyl-2-methylsulfanyl-5-nitro-6-pyridin-4-ylpyrimidin-4-one, 3-Pentadecylphenol, 2-biphenylene carboxylic acid, Quinoline-3-carboxylic acid,and Stigmast-5-en-3-ol are important phytoconstituents which have antipyretic and antiparasitic activities.Conclusion: The present investigation revealed preliminary information on phytocompounds presented in S. jambos leaf extract which is very usefulfor the human community.Keywords: Syzygium jambos, Gas chromatography-mass spectrometry analysis, 1-Deoxy-d-mannitol, Phytoconstituents, Methanolic leaf extract.

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