scholarly journals EFFECT OF GRAPE SEED EXTRACT SOLUTION ON THE MICROHARDNESS OF THE ROOT CANAL DENTIN: AN IN VITRO STUDY

2020 ◽  
pp. 62-65
Author(s):  
SYLVA ALINDA ◽  
ANGGRAINI MARGONO ◽  
DINI ASRIANTI
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Distilled Water ◽  
Root Canal Irrigation ◽  
Significant Difference ◽  
Root Canal Dentin

Objective: Grape seed extract (GSE) containing proanthocyanidin as a root canal irrigation solution has its antibacterial effects and ability to eliminatethe smear layer. In addition, proanthocyanidin acts as a cross-linking agent of collagen, which adds to dentin’s mechanical properties. This studyanalyzed the effect of GSE containing 2.9% proanthocyanidin on the microhardness of the dentin in the root canal.Methods: Fifty teeth were divided into three groups and immersed in GSE solution, 3% NaOCl solution, or distilled water (controls) for 30 min for 3consecutive days. The microhardness was measured using the Vickers method. Data were analyzed using the Kruskal–Wallis test.Results: The GSE group presented the highest microhardness values, whereas the lowest values in the NaOCl group. No significant difference inmicrohardness observed between the GSE and distilled water groups.Conclusion: The GSE solution maintains the microhardness of the root canal dentin.

scholarly journals EFFECT OF GRAPE SEED EXTRACT ON REMINERALIZATION OF ARTIFICIAL CARIES: AN IN-VITRO STUDY

2016 ◽  
Vol 9 (5) ◽  
pp. 174 ◽  
Author(s):  
Rubel M ◽  
Prashant Gm ◽  
Naveen Kumar Pg ◽  
Sushanth Vh ◽  
Mohamed Imranulla ◽  
...  
Keyword(s):  
Dental Caries ◽  
In Vitro Study ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
T Test ◽  
Vitro Study ◽  
Saline Control ◽  
Group Comparison

ABSTRACTObjective: Grape seeds are gaining more importance because the “Proanthocyanidins” present in them have excellent anticaries, remineralizing, andantibacterial properties. Although many preventive measures have been tried, dental caries remains one of the most prevalent diseases in humans.The ultimate goal of any intervention is the preservation of tooth structure, prevention of lesion progression and inducing remineralization. Thepresent in-vitro study was done to investigate the effect of grape seed extract (GSE) on artificial enamel caries in human teeth.Methods: A total of 50 sound premolars extracted from patients undergoing orthodontic treatment were used in the study. The teeth were cleanedand were immersed in a demineralizing solution for 96 hrs at 37°C and 50% relative humidity to create artificial lesions. The samples were randomlydivided into two groups of 25 each. Half of the samples were immersed in the GSE solution (test) and the other half in saline (control), respectively, for8 days. The samples were measured by microhardness tester to obtain values in Vickers hardness scale. Data were entered into Microsoft Excel andwere analyzed using independent t-test for inter-group comparison and paired t-test for intra-group comparison using SPSS version 21.Results: The mean±SD microhardness values after remineralization for the test and control groups were 357.56±5.53 and 337.60±6.38. GSE groupsignificantly increased the microhardness of the lesions (p≤0.001).Conclusion: GSE is a potential remineralizing agent and can be used to prevent progression of dental caries.Keywords: Grape seed extract, Remineralization, Dental caries.

Grape Seed Extract Exerts Abhesive Effect Against Staphylococcus aureus: In vitro Study

2012 ◽  
Vol 7 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Marwan S.M. Al-Ni ◽  
Rana Abd-ul-Karim Rasheed ◽  
Shama Mohamed Jawad Saadaldin
Keyword(s):  
Staphylococcus Aureus ◽  
In Vitro Study ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Vitro Study

scholarly journals The Effects of the Grape Seed Extract on Streptococcus mutans and Candida albicans: An In Vitro Study

2020 ◽  
Vol 12 (2) ◽  
pp. 35-39
Author(s):  
Masoumeh Aslanimehr ◽  
Ashraf Alizadeh ◽  
Faezeh Azmoudeh
Keyword(s):  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Large Population ◽  
In Vitro Study ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Vitis Vinifera L ◽  
Oral Pathogens

Background: Oral pathogens can affect a large population and the general health of individuals. Based on the evidence, the grape seed extract (GSE) contains herbal compounds that have the ability tosuppress oral pathogens associated with caries and fungal diseases. In this regard, the current studyaimed at evaluating the effect of GSE on Streptococcus mutans and Candida albicans. Methods: The grape (Vitis vinifera L.) seed was used in this in-vitro experimental study. After thepreparation of methanolic GSE, its effect on S. mutans and C. albicans was assessed at 0.25-256 mg/mL concentrations. Then, the minimum inhibitory concentration (MIC) and minimum bactericidalconcentration (MBC) were determined by macro broth dilution methods. Finally, data were analyzedby SPSS, version 20. Results: The results showed that GSE inhibits the growth of S. mutans and C. albicans. In addition, theMIC and MBC of the extract against S. mutans and C. albicans were 2 and 8 mg/mL, as well as 32 and64 mg/mL, respectively. Conclusions: In general, GSE had a significant antimicrobial effect on S. mutans, while it only affected C. albicans at high concentrations.

scholarly journals Effectiveness of light-emitting diode exposure on photodynamic therapy against Enterococcus faecalis: in vitro study

2020 ◽  
Vol 53 (2) ◽  
pp. 71
Author(s):  
Nanik Zubaidah ◽  
Agus Subiwahjudi ◽  
Dinda Dewi Artini ◽  
Karina Erda Saninggar
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Pathogenic Bacteria ◽  
In Vitro Study ◽  
Control Group ◽  
Root Canal Irrigation ◽  
Group I ◽  
Significant Difference

Background: A successful root canal treatment eliminates pathogenic bacteria from infected root canals. The most common bacteria in root canal infections is Enterococcus faecalis (E. faecalis), due to its resistance to medicament and root canal irrigation. A photodynamic therapy (PDT) is a method of root canal disinfection that uses a combination of photosensitisers and light activation to eliminate bacteria in the root canal. The duration of the PDT irradiation results in the production of singlet oxygen and reactive oxygen species (ROS) to eliminate the E. faecalis bacteria. Purpose: To analyse the differences in the duration exposure of photodynamic therapy against the E. faecalis bacteria. Methods: The E. faecalis bacteria culture was divided into seven eppendorf tubes. Group I was a control group, and group II, III, IV, V, VI and VII were treated using PDT consisting of Toluidine Blue O (TBO) photosensitiser and light source irradiation for ten, 20, 30, 40, 50 and 60 seconds, respectively. After incubation, the number of bacteria was calculated by the Quebec Colony Counter and analysed using the Kruskal–Wallis test and the Mann–Whitney test (p <0.05). Results: There was a significant difference between the number of E. faecalis bacteria colonies in each treatment group (p <0.05). Group VI and VII, which had a longer exposure to PDT, showed a smaller amount of E. faecalis bacteria. Conclusion: The longer exposure of PDT results in a smaller amount of E. faecalis bacteria. The light irradiation of 50 seconds is the most effective to eliminate E. faecalis bacteria.

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