scholarly journals EFFECT OF PRENATAL ADMINISTRATION OF THERAPEUTIC DOSE OF TOPIRAMATE ON PLACENTAE ALBINO RATS' FETUSES

2017 ◽  
Vol 9 (3) ◽  
pp. 54 ◽  
Author(s):  
Eman Y. Salah El-din ◽  
Amel R. Omar
Keyword(s):  
Caspase 3 ◽  
Periodic Acid ◽  
Treated Group ◽  
Placental Weight ◽  
Control Group ◽  
Albino Rats ◽  
Pregnant Rats ◽  
Predictive Parameters ◽  
Epileptic Patients

Objective: Topiramate is an antiepileptic drug (AED) used for the treatment of partial seizure in adult and children epileptic patients. It passed through the placenta causing a birth defect. However, little literature focused on placental alteration due to the administration of topiramate during pregnancy. So, applying different predictive parameters; placental weight, histopathological (Haematoxylin and Eosin), histochemical (periodic acid Schiff’s) and immunohistochemistry (Caspase-3).Methods: Topiramate was orally administrated to the pregnant rats with dose 100 mg/kg rats from 5th to 19th day of gestation. The dam’s undergone hysterectomy and the placentae were weighted and stained by Haematoxylin and Eosin, periodic acid Schiff’s and Caspase-3. Results: The study indicated that there is statistically significant (P<0.05) increase in the treated placental weight (0.4717±0.03788) compared with control group (0.5208±0.02930). Also, the light microscopic examination of the placental specimens using Haematoxylin and Eosin staining revealed that an alteration in both basal and labyrinth zone. Apoptotic feature in spongiotrophoblast and trophoblasts is detected. Positive Periodic acid Schiff’s reaction for polysaccharides in the topiramate-treated group. Caspase-3 is showing apoptotic cells in the trophoblast layer.Conclusion: Long-term daily use of topiramate during pregnancy can lead to obvious pathological histotoxic effects in layers of placenta tissues which may be implicated in cognitive affection. Various effects of topiramate necessitate further investigations.

scholarly journals Tramadol Induced Adrenal Insufficiency: Histological, Immunohistochemical, Ultrastructural, and Biochemical Genetic Experimental Study

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Shereen Abdelhakim Abdelaleem ◽  
Osama A. Hassan ◽  
Rasha F. Ahmed ◽  
Nagwa M. Zenhom ◽  
Rehab A. Rifaai ◽  
...  
Keyword(s):  
Adrenal Insufficiency ◽  
Thioredoxin Reductase ◽  
Serum Cortisol ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
Narcotic Drug ◽  
Biochemical Genetic ◽  
Withdrawal Effects

Tramadol is a synthetic, centrally acting analgesic. It is the most consumed narcotic drug that is prescribed in the world. Tramadol abuse has dramatically increased in Egypt. Long term use of tramadol can induce endocrinopathy. So, the aim of this study was to analyze the adrenal insufficiency induced by long term use of tramadol in experimental animals and also to assess its withdrawal effects through histopathological and biochemical genetic study. Forty male albino rats were used in this study. The rats were divided into 4 groups (control group, tramadol-treated group, and withdrawal groups). Tramadol was given to albino rats at a dose of 80 mg/kg body weight for 3 months and after withdrawal periods (7–15 days) rats were sacrificed. Long term use of tramadol induced severe histopathological changes in adrenal glands. Tramadol decreased the levels of serum cortisol and DHEAS hormones. In addition, it increased the level of adrenal MDA and decreased the genetic expression of glutathione peroxidase and thioredoxin reductase in adrenal gland tissues. All these changes started to return to normal after withdrawal of tramadol. Thus, it was confirmed that long term use of tramadol can induce severe adrenal insufficiency.

scholarly journals Teratogenic Potential of Solenstemma Argel Extract in Wistar Albino Rats

2021 ◽  
Author(s):  
Nazik M.E. Mustafa ◽  
Nafisa A. Osman ◽  
Shahenaz Satti ◽  
Ahmed A.Gameel ◽  
Tarig M. El-hadiyah
Keyword(s):  
Herbal Medicine ◽  
Fetal Development ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
P Value ◽  
Pregnant Rats ◽  
Histopathological Findings ◽  
Teratogenic Potential ◽  
Wistar Albino Rats

Abstract Background: The majority of people in Africa receive their basic healthcare through herbal treatments. Herbal medicine may negatively impact fetal development irreparably. Methods: This study examined teratogenic potential of Solenstemma argel extract in pregnant rats. pregnant rats were treated with Solenstemma argel from 7th to 16th day of gestation. Dosage used was 500g/kg, intraperitoneal.Results: Solenstemma argel extract treated group showed fetal abnormalities appeared as body clots, limbs abnormalities and Resorption of fetuses that appears in 25% of the fetuses (P-value = 0.01) which is significantly differ from control group. furthermore, Histopathological findings of liver sections from fetuses of Solenstemma argel - treated mothers showed loose liver texture and hepatocyte hemorrhage.Conclusions: In this study we explore the teratogenic potential of S. argel extract during the organogenesis period in pregnant rats.

scholarly journals Teratogenic Potential of Solenstemma Argel Extract in Wistar Albino Rats

2021 ◽  
Author(s):  
Nazik M.E. Mustafa ◽  
Shahenaz Satti ◽  
Nafisa A. Osman ◽  
Ahmed A.Gameel ◽  
Tarig M. El-hadiyah
Keyword(s):  
Fetal Development ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
P Value ◽  
Teratogenic Effects ◽  
Pregnant Rats ◽  
Liver Histopathology ◽  
Teratogenic Potential ◽  
Wistar Albino Rats

Abstract The majority of people in Africa receive their basic health care through herbal treatments. Herbal medicine may negatively impact fetal development irreparably. This study examined the teratogenic potential of Solenstemma argel extract in pregnant rats. Pregnant rats were treated with Solenstemma argel from 7th to 16th day of gestation. The dosage used was 250 mg/kg, intraperitoneal.Solenstemma argel extract treated group showed fetal abnormalities appeared as body hemorrhage, limbs abnormalities and resorption of fetuses. These appears in 25% of the fetuses (P-value = 0.01) which is significantly differed from control group. Furthermore, histopathological findings of liver sections from fetuses of Solenstemma argel - treated mothers showed loose liver texture and hepatocytes hemorrhage.In this study, we conclude that the use Solenstemma argel extract during the organogenesis period in pregnant rats has the potential to cause teratogenic effects, as well as abnormalities in liver histopathology.

scholarly journals Protective effects of Zingiber officinale extract on myocardium and placenta against labetalol-induced histopathological, immune-histochemical, and ultrastructural alterations in pregnant rats

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
Hend T. El-Borm ◽  
Marwa N. Atallah
Keyword(s):  
Caspase 3 ◽  
Zingiber Officinale ◽  
Term Treatment ◽  
Control Group ◽  
Acute Management ◽  
Protective Effects ◽  
Pregnant Rats ◽  
Long Term Treatment ◽  
Maternal Hypertension

Abstract Background Labetalol is an antihypertensive drug commonly used in obstetrics for both long-term treatment and the acute management of severe maternal hypertension. However, there have not been published articles about the effects of labetalol on the myocardium and the placenta. This study aimed to estimate the histological, immune-histochemical, and ultrastructural cardio- and placental-toxicity of labetalol administration and the effectiveness of ginger against this toxicity in pregnant rats. Labetalol was daily administrated orally with or without ginger at a dose of 300 mg/kg and 200 mg/kg, respectively, during the gestation days 6 to 20. Results In the labetalol-administrated group, the myocardium displayed histological and ultrastructure destructive changes and a significant increase in caspase-3 expression. Labetalol also decreased the placental weight compared with the control group, caused marked degeneration and disorganization of their architecture, and increased caspase-3 expression. Co-administration of ginger after labetalol highly ameliorates the adverse effect of labetalol on both cardiac and placental tissues. Conclusions It is concluded that ginger can mitigate cardiac and placental toxicity induced by labetalol administration into pregnant rats.

scholarly journals Long-term scopolamine treatment altered locomotor, exploratory and anxiety-like behaviours of albino rats

2022 ◽  
Vol 11 (1) ◽  
Author(s):  
Asmaa K. Abdelghany ◽  
Akram M. El-Kashlan ◽  
Hosny H. Emeash ◽  
Fatma Khalil
Keyword(s):  
Treated Group ◽  
Inhibitory Effect ◽  
Control Group ◽  
Albino Rats ◽  
Serotonin Level ◽  
Behavioural Patterns ◽  
Significant Difference ◽  
Underlying Processes ◽  
Insight Into

Abstract Background Animal models are used to provide an adequate investigation of brain-behaviour, physiological and path physiological relationships to give insight into human behaviour and the underlying processes of drugs affecting the nervous system. Scopolamine; SCO (alkaloid l-(2)-scopolamine [l-(2)-hyoscine]) has a competitive inhibitory effect on muscarinic receptors for acetylcholine. Thus, this study was designated to investigate the effect of long-term SCO treatment on locomotor, exploratory and anxiety-like behaviours of rats using open field test. Results The long-term SCO treatment induced a prominent increase in locomotion (hyperactivity) and exploratory behaviour of rats. In addition, anxiety-like behavioural patterns showed a non-significant difference in SCO treated compared to control. Serotonin level was significantly decreased in the scopolamine treated group in comparison with the control group. Conclusions Data suggested that long-term SCO treatment resulted in marked neurobehavioural alterations in a rat as an animal model.

scholarly journals A model for the study of skeletal anomalies in rat fetuses

2004 ◽  
Vol 47 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Cristiane Effting ◽  
Daniel Jesus de Paula ◽  
Guilhermino Pereira Nunes Junior
Keyword(s):  
Treated Group ◽  
Placental Weight ◽  
Control Group ◽  
Pregnant Rats ◽  
Rat Fetuses ◽  
Skeletal Anomalies

The aim of this study was to validate a model of skeletal anomalies in rat fetuses by the administration of ketoconazole (80 mg/kg) to pregnant rats during organogenesis. Bones of the head, trunk and anterior and posterior limbs were examined for detection of anomalies. Statistical differences regarding the number of fetuses and postimplantation resorptions, and fetal and placental weight were significant. The frequency of skeletal anomalies in the head, trunk, and anterior and posterior limbs in the ketoconazole-treated group were also significant when compared to the control group. It could be concluded that the model suggested was valid for study of skeletal anomalies and abnormal bones development in rat fetuse, in spite of the loss of fetuses due to resorptions.

scholarly journals Teratogenic Potential of Solenstemma argel Extract in Wistar Albino Rats

2022 ◽  
Author(s):  
Nazik M.E. Mustafa ◽  
Shahenaz Satti ◽  
Nafisa A. Osman ◽  
Ahmed A.Gameel ◽  
Tarig M. El-hadiyah
Keyword(s):  
Fetal Development ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
P Value ◽  
Teratogenic Effects ◽  
Pregnant Rats ◽  
Liver Histopathology ◽  
Teratogenic Potential ◽  
Wistar Albino Rats

Abstract The majority of people in Africa receive their basic health care through herbal treatments. Herbal medicine may negatively impact fetal development irreparably. This study examined the teratogenic potential of Solenstemma argel extract in pregnant rats. Pregnant rats were treated with Solenstemma argel from 7th to 16th day of gestation. The dosage used was 250 mg/kg, intraperitoneal.Solenstemma argel extract treated group showed fetal abnormalities appeared as body hemorrhage, limbs abnormalities and resorption of fetuses. These appears in 25% of the fetuses (P-value = 0.01) which is significantly differed from control group. Furthermore, histopathological findings of liver sections from fetuses of Solenstemma argel - treated mothers showed loose liver texture and hepatocytes hemorrhage.In this study, we conclude that the use Solenstemma argel extract during the organogenesis period in pregnant rats has the potential to cause teratogenic effects, as well as abnormalities in liver histopathology.

scholarly journals Histopathological comparison of the salivary glands’ acini and striated ducts after experimental prolonged daily administration of oral ubiquinone doses in rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ali Ghanim Abdullah ◽  
Ban Ismael Sedeeq ◽  
Marwan Saad Azzubaidi
Keyword(s):  
Salivary Glands ◽  
Prolonged Exposure ◽  
Oral Treatment ◽  
Underlying Mechanism ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
Excessive Salivation ◽  
The Difference

Abstract Also called coenzyme Q10 (CoQ10), Ubiquinone is a vitamin-like endogenously produced factor essential for Adenosine triphosphate (ATP) mitochondrial production. Several research studies have reported that the exogenous supplementation of CoQ10 can lead to excessive salivation, especially in patients complaining of dry mouth. The objective of this study was to investigate the effect of long-term daily use of CoQ10 on the salivary glands in experimental animals by comparing the diameters of the glandular acini and striated ducts of a CoQ10-treated group and a control group. Twenty-five white albino rats were randomly divided into two groups; the control group consisted of 10 rats, while the CoQ10-treated group comprised 15 rats. The latter received daily oral treatment of 300 mg/kg CoQ10 for six weeks. Samples of the parotid, submandibular and sublingual glands were then dissected and examined histologically for comparative measurement of the diameters of the glands’ acini and striated ducts. The CoQ10 treated group had mean diameters of the serous acini for the parotid (79.8±11.2 μm) and submandibular (81.07±13.5 μm) glands that were significantly higher (P<0.05) than their diameters in the control group (67.5±8.4 μm and 73.3±13.8 μm), respectively. However, the difference was not statistically significant when comparing the diameters of striated ducts of the CoQ10-treated group and the control group. Continuous and prolonged exposure to exogenous ubiquinone may cause hypertrophic dilation of the acini within the salivary glands, namely the parotid and submandibular glands, which might be the underlying mechanism for excessive salivation. This can be considered a reversible adaptive response.

scholarly journals Functional and Structural Effects of Erythropoietin Subconjunctival Administration in Glaucomatous Animals

2018 ◽  
Vol 3 (2) ◽  
pp. 1-11 ◽  
Author(s):  
Ana Paula Resende ◽  
Serge G. Rosolen ◽  
Telmo Nunes ◽  
Berta São Braz ◽  
Esmeralda Delgado
Keyword(s):  
Neuroprotective Effect ◽  
Treated Group ◽  
Retinal Function ◽  
Function Evaluation ◽  
Control Group ◽  
Albino Rats ◽  
Subconjunctival Injection ◽  
Beneficial Effects ◽  
Structural Evaluation ◽  
Retinal Structure

Purpose: The present study aimed to assess functional and structural benefits of erythropoietin (EPO) when administered subconjunctivally in the retina of glaucomatous rats using electroretinography (ERG) and retinal thickness (RT) measurements. Methods: Glaucoma was experimentally induced in 26 Wistar Hannover albino rats. Animals were divided into 2 groups of 13 animals each: a treated group receiving a unique subconjunctival injection of 1,000 IU of EPO and a control group receiving a saline solution. In each group, 7 animals were used for retinal function evaluation (ERG) and 6 animals were used for retinal structural evaluation (histology). RT was measured, dorsally and ventrally, at 500 μm (RT1) and at 1,500 μm (RT2) from the optic nerve. Results: Retinal function evaluation: for both scotopic and photopic conditions, ERG wave amplitudes increased in the treated group. This increase was statistically significant (p < 0.05) in photopic conditions. Structural evaluation: for both locations RT1 and RT2, the retinas were significantly (p < 0.05) thicker in the treated group. Conclusion: Subconjunctival EPO administration showed beneficial effects both on retinal structure and on retinal function in induced glaucoma in albino rats. This neuroprotective effect should be applied in other animal species.

scholarly journals Ameliorating Effect of L-arginine and Insulin on Streptozotocin-induced Adrenal Gland Injury in Albino Rats

1969 ◽  
Vol 11 (3) ◽  
pp. 162-168
Author(s):  
Yasmeen Mahar ◽  
Alisha Qamar ◽  
lnayatullah ◽  
Sarwath Fatimee ◽  
Mohammad Fawad Saeeduddin ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
Adrenal Cortex ◽  
Treated Group ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Endocrine Gland ◽  
Protective Effects ◽  
Frozen Sections ◽  
Group B

Background:Use of dietary supplements to treat illnesses has increasedtremendously in recentyears.Adrenal gland is one ofthemost commonly damaged endocrine gland in the body, not only by chemical or radiation injuries, but also as a result of differenttypes of stress.Search is underway for use ofnatural foods for protection of adrenal gland from different types ofinsults.Objective: To determine the protective effects of L-arginine on streptozotocin (STZ)-induced adrenal gland injury in albino rats,andto compare its efficacy to insulin.Material and Methods: This prospective experimental study was done at BMSI, JPMC, Karachi. Forty male, healthy albino rats,90-120 days old were segregated into 4 groups. Group A was marked as control, group B was administered STZ, group C and Dwere treated with STZ along with insulin and L-arginine respectively. At the end of study period, i.e., 6 weeks, animals weresacrificed under ether anaesthesia. Tissue from the left adrenal gland was processed for frozen sectioning to observe fat content ofthe adrenal cortex by applying OilRed O stain.Results: Oil Red-0 stained frozen sections revealed closely aggregated fat globules in adrenal cortex of STZ treated group B ascompared to control. Moderate betterment was seen in group C and in group D Oil Red O stained frozen sections as compared toSTZ treated group B.Conclusion: The results ofthe study demonstrated adrenal cortex injury by STZ which ameliorated with concomitant use of insulinandL-arginine. The protection was more pronounced with L-arginine as comparedto insulin.Keywords:STZ, adrenal gland,insulin,L-arginine

Sign in / Sign up

Export Citation Format

Share Document