scholarly journals ASSESSING COSMECEUTICALS PROPERTIES OF SOME MACROFUNGI FOR IMPROVED SKINCARE

2019 ◽  
pp. 15-19
Author(s):  
AKASH SARASWAT ◽  
PURVI MATHUR ◽  
DOYELI SANYAL
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Sun Protection ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Protection Factor ◽  
Sun Protection Factor ◽  
Ethyl Acetate Extracts

Objective: This study has investigated cosmeceutical properties namely, antibacterial, sun protection factor and total phenolics contents of some selected macrofungi. The studies were conducted on five reference cultures collected from Indian type culture collection center (IMTEC, Chandigarh, India) and five isolates collected from TERI-Deakin Nano Biotechnology Centre facility at TERI Gram, Gurugram, India. Methods: The cosmeceutical properties of the crude extracts from selected macrofungi were analyzed using standard bioassay techniques. Antibacterial activity was analyzed against Staphylococcus epidermidis, Escherichia coli, Micrococcus luteus, Bacillus megaterium, Pseudomonas aeruginosa, Staphylococcus aureus, and Acinetobacter baumannii using Agar well diffusion method. The sun protection factor was estimated and calculated using the Mansur equation. Free radical scavenging activity using DPPH was performed to assess the antioxidant activity of the extracts. Results: Ethyl acetate extracts of the broth from P. florida and TERI-G1 cultures showed a broad-spectrum antibacterial activity against S. epidermidis, E. coli, M. luteus and B. megaterium. Ethyl acetate extracts of the broth from TERI-G3 showed the highest SPF activity of 34.02 at 200 µg. ml-1 concentration. Ethyl acetate extract of the broth from F. velutipes, P. florida, P. ostreatus, and TERI-G1 showed comparable antioxidant activity of 66.86%, 79.51%, 82.02%, and 69.58% respectively when compared to ascorbic acid (85.83%) and quercetin (83.09%) taken as positive control in the study and their total phenolic contents were found to be 6.93, 43.68, 20.88 and 13.77 Gallic acid equivalent (GAE) per gram. The minimal inhibitory concentration of F. velutipes, P. florida, P. ostreatus, and TERI-G1 was found to be 3552.89 µg. ml-1, 1250 µg. ml-1, 2418.9 µg. ml-1 and 3219 µg. ml-1 respectively. Conclusion: The work is in progress to identify and characterize TERI-G1and TERI G3 cultures. Further studies on the anti-inflammatory, anti-tyrosinase, elastase inhibition properties of the cultures will be assessed to identify potential cosmeceutical active ingredients with promising applications in cosmeceutical products.

scholarly journals Melientha suavis Pierre. Extract: Antioxidant and Sunscreen Properties for Future Cosmetic Development

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Pahol Sansomchai ◽  
◽  
Kanlaya Jumpatong ◽  
Chaipat Lapinee ◽  
Kanchana Utchariyajit ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Sun Protection ◽  
Total Phenolic ◽  
The Sun ◽  
Protection Factor ◽  
Abts Assay ◽  
Ms Analysis ◽  
Sun Protection Factor

The ultimate aim of this study was to evaluate antioxidant activities using DPPH and ABTS assay and antioxidant substances of Melientha suavis Pierre (Melientha) extracts with ethanol (EtOH) and subsequent partition with hexane, dichloromethane (CH2Cl2), ethyl acetate (EtOAc) and distilled water (W). The Melientha extract was assessed the sunscreen activities by sun protection factor which was compared with octyldimethyl PABA. Additionally, the stability test of Melientha extracts added in the cosmetic base-formula was investigated. The sun protection substances of Melientha extracts were determined by GC-MS analysis. Among the extract and fractions, the ethanolic extract showed the highest activities. The IC50 of Melientha extract to free radical scavenging determined by DPPH assay and ABTS assay were 53.20 ± 7.37 μg/ml and 64.17 ± 5.76 μg/ml, respectively. The total phenolic compound of ethanol extract was 149.87 ± 2.72 mg GE/g of ext and total flavonoid content was 51.60 ± 4.12 mg CE/g of ext. The sun protection factor (SPF) of ethanol extract was 26.61 ± 0.10. Cinamate and its derivative which were claim as sunscreen substance was found by GC-MS analysis of Melientha ethanol extract.

scholarly journals Antibacterial Potential of some Plant Extracts against Escherichia coli, along with its Phytochemical Analysis, Total Phenolic Content, and Antioxidant Properties

2019 ◽  
Vol 5 (02) ◽  
pp. 96-102
Author(s):  
Pooja Singh ◽  
Upma Srivastava
Keyword(s):  
Escherichia Coli ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis

In vitro antibacterial activity of 21 plant methanolic extracts were investigated by disc diffusion method against gram-negative bacteria Escherichia coli. Amongst the extracts tested, the Datura stramonium L. and Ocimum basilicum L. showed significant antibacterial activity against the bacterial pathogen. D. stramonium showed the highest antibacterial activity followed by O. basilicum extract. Phytochemical analysis of extracts showed the presence of alkaloids, tannins, saponins, flavonoids, and phenols. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ABTS analysis of potent extract D. stramonium showed appreciable antioxidant activity. For DPPH and ABTS assay the highest antioxidant activity was observed at 60 mg ml-1 concentration with a percent inhibition of 71.66 and 77.19 with IC50 value 9.71 and 22.16 μg ml-1 respectively. Moreover, the radical scavenging activity of the extract was lower than that observed for the synthetic antioxidants butylated hydroxyanisole (BHA )and butylated hydroxytoluene (BHT). The total phenolic content of D. stramonium extract was found to be 0.592 mg GAE/gm which was assayed using Folin ciocalteau reagent. The results provide evidence that the extract of D. stramonium and O. basilicum can be further recommended in the treatment of the infections caused by E. coli; further D. stramonium is also a potential source of natural antioxidants.

Chemical composition, antioxidant, alpha-glucosidase inhibitory, anticholinesterase and photoprotective activities of the aerial parts of Schinus molle L.

2020 ◽  
Vol 16 ◽  
Author(s):  
Mustapha M. Bouhenna ◽  
Chawki Bensouici ◽  
Latifa Khattabi ◽  
Farid Chebrouk ◽  
Nabil Mameri
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Sun Protection ◽  
Antidiabetic Activity ◽  
Protection Factor ◽  
Schinus Molle ◽  
Rp Hplc ◽  
Sun Protection Factor

Background: Schinus molle L. is a medicinal and aromatic plant widely used in folk medicine commonly found in Algeria and Mediterranean region. In the present work, we investigated the in vitro antioxidant, anti-cholinesterase, αglucosidase inhibitory and photoprotective potentials of the Schinus molle L. plant’s extract/fractions. The metabolite profile of Schinus molle L. was analyzed using RP-HPLC and GC-MS. Methods: Antioxidant activity was assessed using different tests. Anticholinesterase activity was investigated by quantifying the acetylcholinesterase and butyrylcholinesterase inhibitory activities. Antidiabetic activity was investigated by αglucosidase inhibition test, whereas photoprotective activity was evaluated by sun protection factor (SPF) using spectrophotometry UV-Vis. The extracts were then submitted to RP-HPLC–PDA and GC–MS analysis. Results: Ethyl acetate and buthanol fractions with high contents of total phenolics and flavonoids exhibited the strongest antioxidant activity. Ethyl acetate extract exhibited considerable enzyme inhibition potential on acetylcholinesterase, butyrylcholinesterase and α-glucosidase with percentages of inhibition of 99.08 ± 0.79 %, 100 % and 98.80 ± 0.18 % respectively. Furthermore, the ethyl acetate fraction showed high photoprotective activity with the sun protection factor (SPF) value = 38,26 ± 0.73. Three phenolic acids (gallic, tannic and vanillic acids) and five flavonoids (myricetin, apigenin, naringenin, rutin and quercetin) were identified with RP-HPLC–PDA. Conclusion: These findings suggest that Schinus molle L. may be useful in the development of an alternative agent for oxidative stress, Alzheimer’s disease, diabetes and could be used as a natural sunscreen in pharmaceutics or cosmetic formulations.

scholarly journals Sun protection factor, total phenol, flavonoid contents and antioxidant activity of medicinal plants from Iran

2021 ◽  
Vol 18 (7) ◽  
pp. 1443-1448
Author(s):  
Zahra Hashemi ◽  
Mohammad Ali Ebrahimzadeh ◽  
Masoumeh Khalili
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Sun Protection ◽  
Protection Factor ◽  
Artemisia Absinthium ◽  
Sun Protection Factor

Purpose: To evaluate the correlation between sun protection factor (SPF) and the content of phenol and flavonoid and antioxidant activity. Methods: Different parts of 9 medicinal plants were extracted with methanol using three extraction methods (percolation, Soxhlet and ultrasonically assisted extraction) to obtain 42 crude extracts. Their phenol and flavonoid contents, and antioxidant activities were determined using Folin-Ciocalteu reagent, aluminum chloride method and DPPH radical-scavenging activity, respectively. The SPF values were determined and correlated with the phenol and flavonoid contents as well as antioxidant activities. Results: The phenol and flavonoid contents, and antioxidant activities ranged from 54.16-688.97mg GAE/g, 13.38-146.60 mg QE/g and 9.5-1472.4 µg/mL, respectively while the SPFs were between 0.067 and 0.841. The highest SPF was related to Cucumis melo L. ultrasonically assisted leaf extract (0.841) and Artemisia absinthium L., aerial parts extracted with percolation method (0.717). A significant correlation was found between SPF and phenolic (p= 0.003) and flavonoid contents (p= 0.023). Conclusion: This study showed a correlation between SPF and phenolic and flavonoid contents. Ultrasonically assisted extract of C. melo leaf has suitable SPF and can be used in sun screen formulations.

Phenolic Content, Antioxidant Properties, Key Enzyme Inhibitory Potential and Photoprotective Activity of Lawsonia inermis L

2020 ◽  
Vol 16 ◽  
Author(s):  
Rima Goudjil ◽  
Abdelkader Mekhaldi ◽  
Houari Benamar ◽  
Chawki Bensouici ◽  
Mohamed Amine Kahoul
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Enzyme Inhibition ◽  
Methanol Extract ◽  
Biological Activities ◽  
Sun Protection ◽  
Bioactive Molecules ◽  
Lawsonia Inermis ◽  
Protection Factor ◽  
Sun Protection Factor

Background:: Lawsonia inermis Linn (Lythraceae), commonly known as "Henna" is a medicinal plant widely used as a folk remedy and for its cosmetic properties. Objective:: The objective of this present work was to evaluate biological activities and to quantify phenolics in extracts. Methods:: The extracts were obtained from seeds of L. inermis by increasing the polarity of the solvent. The content of total phenolics, flavonoids, flavonols and tannins was determined using colorimetric methods. Also, to evaluate the antioxidant activity, six different assays, DPPH, ABTS, superoxide radical scavenging, inhibition of β-carotene bleaching, ferric reducing antioxidant power and phenanthroline assays were used. Enzyme inhibition activity was evaluated by acetylcholinesterase, butyrylcholinesterase and tyrosinase inhibition assays. Furthermore, photoprotective activity was determined by measuring sun protection factor. Results:: The extraction efficiency of phenolics, as well as the biological activities of plant extracts, were affected by solvent polarity. The highest content of phenolics was determined in methanol extract (786.54 ± 0.00 mg GAEg-1 DW), followed by the aqueous and ethyl acetate extracts (526.48 ± 0.40 and 331.25 ± 0.00 GAEg-1 DW, respectively). The above extracts also exhibited the highest antioxidant activity, while low polar extracts were characterized with the lowest content of phenolics, as well as the lowest antioxidant capacity. The highest enzyme inhibition activities were found in ethyl acetate extract. Moreover, the methanol extract showed the best photoprotective activity with sun protection factor of 43.05 ± 0.37. Conclusion:: These findings suggest a possible use of Henna seeds as a potential source of bioactive molecules with antioxidant, enzyme inhibition and skin protection properties.

scholarly journals Antioxidant and antibacterial activity of Acorus calamus. L leaf and rhizome extracts

2017 ◽  
Vol 13 (4) ◽  
pp. 144 ◽  
Author(s):  
Kho See Li ◽  
Chan Sook Wah
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Acorus Calamus ◽  
Methanol Extracts ◽  
Antioxidant Agents ◽  
Sweet Flag

Background: Acorus calamus (sweet flag) is a well-known traditional herb used in India. There were only limited studies done on both hydrophilic and hydrophobic extracts of A. calamus and the biological activities of phenolic and flavonoid compounds as well as alpha-asarone in the sweet flag.Objective: The present study was designed to explore the antioxidant and antibacterial activities of both hydrophilic and hydrophobic of A. calamus leaf and rhizome extracts. Lastly, the correlation between the biological activities and total phenolic content (TPC), total flavonoid content (TFC) as well as total alpha-asarone content (TAC) of three different extracts (water, hexane and methanol extracts) was revealed.Method: TAC was determined through using HPLC method, while TPC and TFC were determined through spectrophotometric method. DPPH-free radical scavenging assay (DPPH-FRS) and ferric reducing power assay (FRAP) were used to analyze antioxidant activity, whereby disc diffusion method was used to analyze the antibacterial activity.Results: Methanol extracts had significantly (p<0.05) highest levels of antioxidant activities, antibacterial activity TPC (4.466%1; 3.603%2) and TFC (12.819%1; 7.411%2) content. The significant (p<0.05) highest TAC was observed in hexane extracts (0.762%1; 2.975%2). TPC (p<0.05; r=0.855) and TFC (p<0.05; r=0.855) correlated to FRAP, while DPPH-FRS solely correlated to TFC (p<0.05; r=0.873).Conclusion: Both of the hydrophilic and hydrophobic extracts of A. calamus could be potent antioxidant agents for nutraceutical application and antibacterial agent for food application. Lastly, flavonoids and phenols extracted from sweet flag could be a good potent source of antioxidants for nutraceuticals or food additives as those compounds are correlated to the antioxidant activity.1 Leaf extract; 2Rhizome extract

scholarly journals Study on Biological Activity of Litsea monopetala From Panchthar District of Nepal

2020 ◽  
Vol 25 (2) ◽  
pp. 113-118
Author(s):  
Subash Khanal ◽  
Bishan Datt Bhatt
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Bioactive Substances ◽  
Ic50 Values ◽  
Acetone Extracts ◽  
Dpph Radical Scavenging

The Panchthar, a hilly district of Eastern Nepal, has several medicinal plants which are used by indegenous people for the treatments of different diseases. In the present work, phytochemical and biological activities of hexane, ethyl acetate, acetone, and methanol extracts of Litsea monopetala leaves from the Pachthar district were analyzed. The phytochemical screening demonstrated the presence of major secondary metabolites like flavonoids, alkaloids, terpenoids, polyphenols, glycosides, and proteins. The antibacterial activity was estimated by the disc diffusion method against Bacillus substilus and Escherichia coli. The result showed that ethyl acetate extract exhibit the highest zone of inhibition (ZOI) value of 18 mm/disc. Acetone extract also showed significant antibacterial activity at the concentration of 100 mg/mL. Antioxidant activity from DPPH radical scavenging assay displayed the inhibitory concentration (IC50) values of dried methanol and acetone extracts were 30.74 μg/mL and 202.86 μg/mL, respectively. The methanol extract has the highest total phenolic content of 360.14 mg GAE/g extract and the highest total flavonoid content of 27.13 mg QE/g extract. The present study revealed that Litsea monopetala plant from the Panchthar district is a source of several bioactive substances and can be used in several drugs formulation.

scholarly journals Antioxidant Activity in Rheum emodi Wall (Himalayan Rhubarb)

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2555
Author(s):  
Sang Koo Park ◽  
Yoon Kyung Lee
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Polyphenol Content ◽  
Abts Assay ◽  
Ic50 Values

Using natural products as antioxidant agents has been beneficial to replace synthetic products. Efforts have been made to profile the antioxidant capacities of natural resources, such as medicinal plants. The polyphenol content of Himalayan rhubarb, Rheum emodi wall, was measured and the antioxidant activity was determined using DPPH and ABTS+ assay, and the oxidative stress was assessed using SOD enzymatic assay. Five different solvent fractions, n-hexane, n-butanol, ethyl acetate, dichloromethane, and water, were used for screening the antioxidant capacity in effort to determine the optimum extraction solvent. The total phenolic contents for R. emodi fractions ranged from 27.76 to 209.21 mg of gallic acid equivalents (GAE)/g of dry weight. DPPH and ABTS+ assay results are presented into IC50 values, ranged from 21.52 to 2448.79 μg/mL and 90.25 to 1718.05 μg/mL, respectively. The ethyl acetate fraction had the highest antioxidant activity among other fractions. Also, n-butanol and water fractions showed significantly lower IC50 values than the positive control in DPPH radical scavenging activity. The IC50 values of SOD assay of fractions ranged from 2.31 to 64.78 μg/mL. A similar result was observed with ethyl acetate fraction showing the highest SOD radical scavenging activity. The study suggests that the ethyl acetate fraction of R. emodi possess the strongest antioxidant activity, thus the most efficient in extracting antioxidant contents. Moreover, a highly significant correlation was shown between total polyphenol content and antioxidant activity screening assays. The compounds related to the antioxidant activity of R. emodi were identified to myricitrin, myricetin 3-galloyl rhamnoside, and myricetin, which have not been reported in studies about R. emodi before.

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

scholarly journals Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
K. Nagendra Prasad ◽  
Jing Hao ◽  
Chun Yi ◽  
Dandan Zhang ◽  
Shengxiang Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Scavenging Activity ◽  
Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

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