Chemical composition, antioxidant, alpha-glucosidase inhibitory, anticholinesterase and photoprotective activities of the aerial parts of Schinus molle L.

2020 ◽  
Vol 16 ◽  
Author(s):  
Mustapha M. Bouhenna ◽  
Chawki Bensouici ◽  
Latifa Khattabi ◽  
Farid Chebrouk ◽  
Nabil Mameri
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Sun Protection ◽  
Antidiabetic Activity ◽  
Protection Factor ◽  
Schinus Molle ◽  
Rp Hplc ◽  
Sun Protection Factor

Background: Schinus molle L. is a medicinal and aromatic plant widely used in folk medicine commonly found in Algeria and Mediterranean region. In the present work, we investigated the in vitro antioxidant, anti-cholinesterase, αglucosidase inhibitory and photoprotective potentials of the Schinus molle L. plant’s extract/fractions. The metabolite profile of Schinus molle L. was analyzed using RP-HPLC and GC-MS. Methods: Antioxidant activity was assessed using different tests. Anticholinesterase activity was investigated by quantifying the acetylcholinesterase and butyrylcholinesterase inhibitory activities. Antidiabetic activity was investigated by αglucosidase inhibition test, whereas photoprotective activity was evaluated by sun protection factor (SPF) using spectrophotometry UV-Vis. The extracts were then submitted to RP-HPLC–PDA and GC–MS analysis. Results: Ethyl acetate and buthanol fractions with high contents of total phenolics and flavonoids exhibited the strongest antioxidant activity. Ethyl acetate extract exhibited considerable enzyme inhibition potential on acetylcholinesterase, butyrylcholinesterase and α-glucosidase with percentages of inhibition of 99.08 ± 0.79 %, 100 % and 98.80 ± 0.18 % respectively. Furthermore, the ethyl acetate fraction showed high photoprotective activity with the sun protection factor (SPF) value = 38,26 ± 0.73. Three phenolic acids (gallic, tannic and vanillic acids) and five flavonoids (myricetin, apigenin, naringenin, rutin and quercetin) were identified with RP-HPLC–PDA. Conclusion: These findings suggest that Schinus molle L. may be useful in the development of an alternative agent for oxidative stress, Alzheimer’s disease, diabetes and could be used as a natural sunscreen in pharmaceutics or cosmetic formulations.

Phenolic Content, Antioxidant Properties, Key Enzyme Inhibitory Potential and Photoprotective Activity of Lawsonia inermis L

2020 ◽  
Vol 16 ◽  
Author(s):  
Rima Goudjil ◽  
Abdelkader Mekhaldi ◽  
Houari Benamar ◽  
Chawki Bensouici ◽  
Mohamed Amine Kahoul
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Enzyme Inhibition ◽  
Methanol Extract ◽  
Biological Activities ◽  
Sun Protection ◽  
Bioactive Molecules ◽  
Lawsonia Inermis ◽  
Protection Factor ◽  
Sun Protection Factor

Background:: Lawsonia inermis Linn (Lythraceae), commonly known as "Henna" is a medicinal plant widely used as a folk remedy and for its cosmetic properties. Objective:: The objective of this present work was to evaluate biological activities and to quantify phenolics in extracts. Methods:: The extracts were obtained from seeds of L. inermis by increasing the polarity of the solvent. The content of total phenolics, flavonoids, flavonols and tannins was determined using colorimetric methods. Also, to evaluate the antioxidant activity, six different assays, DPPH, ABTS, superoxide radical scavenging, inhibition of β-carotene bleaching, ferric reducing antioxidant power and phenanthroline assays were used. Enzyme inhibition activity was evaluated by acetylcholinesterase, butyrylcholinesterase and tyrosinase inhibition assays. Furthermore, photoprotective activity was determined by measuring sun protection factor. Results:: The extraction efficiency of phenolics, as well as the biological activities of plant extracts, were affected by solvent polarity. The highest content of phenolics was determined in methanol extract (786.54 ± 0.00 mg GAEg-1 DW), followed by the aqueous and ethyl acetate extracts (526.48 ± 0.40 and 331.25 ± 0.00 GAEg-1 DW, respectively). The above extracts also exhibited the highest antioxidant activity, while low polar extracts were characterized with the lowest content of phenolics, as well as the lowest antioxidant capacity. The highest enzyme inhibition activities were found in ethyl acetate extract. Moreover, the methanol extract showed the best photoprotective activity with sun protection factor of 43.05 ± 0.37. Conclusion:: These findings suggest a possible use of Henna seeds as a potential source of bioactive molecules with antioxidant, enzyme inhibition and skin protection properties.

The cosmetic potential of the medicinal halophyte Tamarix gallica L. (Tamaricaceae) growing in the eastern of Algeria: photoprotective and antioxidant activities

2020 ◽  
Vol 23 ◽  
Author(s):  
Mostefa Lefahal ◽  
El-Hani Makhloufi ◽  
Warda Trifa ◽  
Radia Ayad ◽  
Mohamed El Hattab ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Total Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Protection Factor ◽  
Total Antioxidant ◽  
Sun Protection Factor ◽  
Phenolic And Flavonoid ◽  
Tamarix Gallica

Aim and Objective: Currently, the use of ingredients from natural sources has gained great attention in cosmetic field especially for the development of new photoprotective formulations. Therefore, the present study aimed to evaluate the cosmetic potential of the methanolic crude extract and the ethyl acetate fraction of the medicinal halophyte Tamarix gallica (Tg) growing in the area of Tebessa in eastern of Algeria, by assessing their phenolic and flavonoid contents, photoprotective and antioxidant activities. Methods: The research approach consisted of determination phenolic and flavonoid contents of aerial parts via FolinCiocalteu and aluminum chloride methods respectively. The antioxidant activity was measured through two in vitro methods DPPH radical scavenging activity and total antioxidant capacity test (TAC). The in vitro photoprotective effect was performed according to the parameter SPF (Sun Protection Factor) by using UV spectroscopic method in UV-B region (290-320 nm). Results: The methanolic extract (Tg-MeOH) and ethyl acetate (Tg-EtOAc) fraction showed good antioxidant activity with IC50’s 14,05±0,66, 27,58±1,98 µg/mL respectively in DPPH test. Furthermore, both extracts displayed strong total antioxidant capacity (287.01±7.85, 246.7±1.12 mg AAE/g, respectively) in TAC test. Both extracts exhibited high photoprotective activity, with sun protection factor (SPF) values 37.03±0.22 and 36.08±0.03. The antioxidant and photoprotective activities of these extracts is probably related to polyphenols content (190.27±0.74 mg AGE /g and 121.77±1.29 mg AGE /g, respectively) , and flavonoids (78.75±0.06 mg QE /g and 58.67±1.19mg/g). Conclusion: Our finding show that extracts of Tamarix gallica L could be a promising source to be mixed as natural sunscreens and antioxidants agent into photoprotective cosmetic formulations.

scholarly journals ASSESSING COSMECEUTICALS PROPERTIES OF SOME MACROFUNGI FOR IMPROVED SKINCARE

2019 ◽  
pp. 15-19
Author(s):  
AKASH SARASWAT ◽  
PURVI MATHUR ◽  
DOYELI SANYAL
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Sun Protection ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Protection Factor ◽  
Sun Protection Factor ◽  
Ethyl Acetate Extracts

Objective: This study has investigated cosmeceutical properties namely, antibacterial, sun protection factor and total phenolics contents of some selected macrofungi. The studies were conducted on five reference cultures collected from Indian type culture collection center (IMTEC, Chandigarh, India) and five isolates collected from TERI-Deakin Nano Biotechnology Centre facility at TERI Gram, Gurugram, India. Methods: The cosmeceutical properties of the crude extracts from selected macrofungi were analyzed using standard bioassay techniques. Antibacterial activity was analyzed against Staphylococcus epidermidis, Escherichia coli, Micrococcus luteus, Bacillus megaterium, Pseudomonas aeruginosa, Staphylococcus aureus, and Acinetobacter baumannii using Agar well diffusion method. The sun protection factor was estimated and calculated using the Mansur equation. Free radical scavenging activity using DPPH was performed to assess the antioxidant activity of the extracts. Results: Ethyl acetate extracts of the broth from P. florida and TERI-G1 cultures showed a broad-spectrum antibacterial activity against S. epidermidis, E. coli, M. luteus and B. megaterium. Ethyl acetate extracts of the broth from TERI-G3 showed the highest SPF activity of 34.02 at 200 µg. ml-1 concentration. Ethyl acetate extract of the broth from F. velutipes, P. florida, P. ostreatus, and TERI-G1 showed comparable antioxidant activity of 66.86%, 79.51%, 82.02%, and 69.58% respectively when compared to ascorbic acid (85.83%) and quercetin (83.09%) taken as positive control in the study and their total phenolic contents were found to be 6.93, 43.68, 20.88 and 13.77 Gallic acid equivalent (GAE) per gram. The minimal inhibitory concentration of F. velutipes, P. florida, P. ostreatus, and TERI-G1 was found to be 3552.89 µg. ml-1, 1250 µg. ml-1, 2418.9 µg. ml-1 and 3219 µg. ml-1 respectively. Conclusion: The work is in progress to identify and characterize TERI-G1and TERI G3 cultures. Further studies on the anti-inflammatory, anti-tyrosinase, elastase inhibition properties of the cultures will be assessed to identify potential cosmeceutical active ingredients with promising applications in cosmeceutical products.

In Vitro Evaluation of Sun Protection Factor of Plant Extracts By Ultraviolet Spectroscopy Method

2021 ◽  
Vol 1 (30) ◽  
pp. 58-62
Author(s):  
Geeta Bhandari Garima Negi
Keyword(s):  
Chronic Exposure ◽  
Sun Protection ◽  
Skin Protection ◽  
Murraya Koenigii ◽  
Protection Factor ◽  
Safe Alternative ◽  
Sun Protection Factor ◽  
Acute And Chronic Exposure ◽  
Sunscreen Products

Abstract-Acute and chronic exposure to nonphysiological doses of ultraviolet radiation leads to variety of changes of skin ranging from sun-burn, erythema to skin cancer. For skin protection from deleterious effects of sunlight, sunscreen products are used in various forms having chemical and physical filters. However, synthetic sunscreen formulations are reported to cause adverse effects. Thus the present study was planned to evaluate the sun protection factor (SPF) of leaves extract of Murraya koenigii for their application as herbal sunscreens. The results suggested that the occurrence of active compounds responsible for ultraviolet absorption can be extracted for use in sunscreens preparations as better, cheaper and safe alternative to harmful chemical sunscreens. Keywords: UV radiations, SPF, Murraya koenigii

scholarly journals Pengaruh Jenis Pelarut Terhadap Nilai Sun Protection Factor Maserat Daun Kelor

2019 ◽  
Vol 1 (1) ◽  
pp. 9-13
Author(s):  
Venny Diah Ningsih ◽  
Isma Oktadiana
Keyword(s):  
Antioxidant Activity ◽  
Experimental Research ◽  
Research Method ◽  
Descriptive Analysis ◽  
Sun Protection ◽  
Protection Factor ◽  
Analysis Design ◽  
Sun Protection Factor

Moringa leaves are one part of the plant which has many health properties one of which is an antioxidant. One of the content of Moringa leaves which contains antioxidant activity is quercetin. Quercetin Is polar and insoluble in air. An experimental research method with a descriptive analysis design, starting with making Moringa leaf maserat, screening, and testing the SPF value, using a UV-Vis spectrophotometer. The purpose of this research was to find out the type of solvent against the SPF value of Moringa leaf maserat. Based on the experiments that have been carried out the methanol (6.75) solvent elds a higher SPF value compared to ethanol (5.32).   Keyword: Moringa Leaf, Solvent, SPF. ABSTRAK   Daun kelor merupakan salah satu bagian tanaman yang memiki banyak khasiat untuk kesehatan salah satunya ialah sebagai antioksidan. Salah satu kandungan daun kelor yang memiliki aktivitas sebagai antioksidan aialah quercetin. Quercetin Bersifat polar dan tidak larut dalam air. Metode penelitian eksperimental dengan desain analisis deskriptif, dimulai dengan pembuatan maserat daun keor, penyaringan, dan pengujian Nilai SPF, menggunakan Spektrofotometer UV-Vis. Tujuan dari penelitian ini ialah untuk mengetahui pengaruh jenis pelarut terhadap nilai SPF maserat daun kelor. Berdasarkan percobaan yang telah dilakukan didaptkan hasil bahawa pelarut methanol (6,75) memberikan nilai SPF lebih tinggi dibandingkan dengan dengan pelarut etanol (5.32).   Kata Kunci : Daun Kelor, Pelarut, SPF

Correlation of in vivo and in vitro measurements of sun protection factor

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
M.-T. Sheu ◽  
C.-W. Lin ◽  
M.-C. Huang ◽  
C.-H. Shen ◽  
H.-O. Ho
Keyword(s):  
Sun Protection ◽  
Protection Factor ◽  
In Vitro Measurements ◽  
Sun Protection Factor
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