Phenolic Content, Antioxidant Properties, Key Enzyme Inhibitory Potential and Photoprotective Activity of Lawsonia inermis L

2020 ◽  
Vol 16 ◽  
Author(s):  
Rima Goudjil ◽  
Abdelkader Mekhaldi ◽  
Houari Benamar ◽  
Chawki Bensouici ◽  
Mohamed Amine Kahoul
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Enzyme Inhibition ◽  
Methanol Extract ◽  
Biological Activities ◽  
Sun Protection ◽  
Bioactive Molecules ◽  
Lawsonia Inermis ◽  
Protection Factor ◽  
Sun Protection Factor

Background:: Lawsonia inermis Linn (Lythraceae), commonly known as "Henna" is a medicinal plant widely used as a folk remedy and for its cosmetic properties. Objective:: The objective of this present work was to evaluate biological activities and to quantify phenolics in extracts. Methods:: The extracts were obtained from seeds of L. inermis by increasing the polarity of the solvent. The content of total phenolics, flavonoids, flavonols and tannins was determined using colorimetric methods. Also, to evaluate the antioxidant activity, six different assays, DPPH, ABTS, superoxide radical scavenging, inhibition of β-carotene bleaching, ferric reducing antioxidant power and phenanthroline assays were used. Enzyme inhibition activity was evaluated by acetylcholinesterase, butyrylcholinesterase and tyrosinase inhibition assays. Furthermore, photoprotective activity was determined by measuring sun protection factor. Results:: The extraction efficiency of phenolics, as well as the biological activities of plant extracts, were affected by solvent polarity. The highest content of phenolics was determined in methanol extract (786.54 ± 0.00 mg GAEg-1 DW), followed by the aqueous and ethyl acetate extracts (526.48 ± 0.40 and 331.25 ± 0.00 GAEg-1 DW, respectively). The above extracts also exhibited the highest antioxidant activity, while low polar extracts were characterized with the lowest content of phenolics, as well as the lowest antioxidant capacity. The highest enzyme inhibition activities were found in ethyl acetate extract. Moreover, the methanol extract showed the best photoprotective activity with sun protection factor of 43.05 ± 0.37. Conclusion:: These findings suggest a possible use of Henna seeds as a potential source of bioactive molecules with antioxidant, enzyme inhibition and skin protection properties.

Chemical composition, antioxidant, alpha-glucosidase inhibitory, anticholinesterase and photoprotective activities of the aerial parts of Schinus molle L.

2020 ◽  
Vol 16 ◽  
Author(s):  
Mustapha M. Bouhenna ◽  
Chawki Bensouici ◽  
Latifa Khattabi ◽  
Farid Chebrouk ◽  
Nabil Mameri
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Sun Protection ◽  
Antidiabetic Activity ◽  
Protection Factor ◽  
Schinus Molle ◽  
Rp Hplc ◽  
Sun Protection Factor

Background: Schinus molle L. is a medicinal and aromatic plant widely used in folk medicine commonly found in Algeria and Mediterranean region. In the present work, we investigated the in vitro antioxidant, anti-cholinesterase, αglucosidase inhibitory and photoprotective potentials of the Schinus molle L. plant’s extract/fractions. The metabolite profile of Schinus molle L. was analyzed using RP-HPLC and GC-MS. Methods: Antioxidant activity was assessed using different tests. Anticholinesterase activity was investigated by quantifying the acetylcholinesterase and butyrylcholinesterase inhibitory activities. Antidiabetic activity was investigated by αglucosidase inhibition test, whereas photoprotective activity was evaluated by sun protection factor (SPF) using spectrophotometry UV-Vis. The extracts were then submitted to RP-HPLC–PDA and GC–MS analysis. Results: Ethyl acetate and buthanol fractions with high contents of total phenolics and flavonoids exhibited the strongest antioxidant activity. Ethyl acetate extract exhibited considerable enzyme inhibition potential on acetylcholinesterase, butyrylcholinesterase and α-glucosidase with percentages of inhibition of 99.08 ± 0.79 %, 100 % and 98.80 ± 0.18 % respectively. Furthermore, the ethyl acetate fraction showed high photoprotective activity with the sun protection factor (SPF) value = 38,26 ± 0.73. Three phenolic acids (gallic, tannic and vanillic acids) and five flavonoids (myricetin, apigenin, naringenin, rutin and quercetin) were identified with RP-HPLC–PDA. Conclusion: These findings suggest that Schinus molle L. may be useful in the development of an alternative agent for oxidative stress, Alzheimer’s disease, diabetes and could be used as a natural sunscreen in pharmaceutics or cosmetic formulations.

scholarly journals ASSESSING COSMECEUTICALS PROPERTIES OF SOME MACROFUNGI FOR IMPROVED SKINCARE

2019 ◽  
pp. 15-19
Author(s):  
AKASH SARASWAT ◽  
PURVI MATHUR ◽  
DOYELI SANYAL
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Sun Protection ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Protection Factor ◽  
Sun Protection Factor ◽  
Ethyl Acetate Extracts

Objective: This study has investigated cosmeceutical properties namely, antibacterial, sun protection factor and total phenolics contents of some selected macrofungi. The studies were conducted on five reference cultures collected from Indian type culture collection center (IMTEC, Chandigarh, India) and five isolates collected from TERI-Deakin Nano Biotechnology Centre facility at TERI Gram, Gurugram, India. Methods: The cosmeceutical properties of the crude extracts from selected macrofungi were analyzed using standard bioassay techniques. Antibacterial activity was analyzed against Staphylococcus epidermidis, Escherichia coli, Micrococcus luteus, Bacillus megaterium, Pseudomonas aeruginosa, Staphylococcus aureus, and Acinetobacter baumannii using Agar well diffusion method. The sun protection factor was estimated and calculated using the Mansur equation. Free radical scavenging activity using DPPH was performed to assess the antioxidant activity of the extracts. Results: Ethyl acetate extracts of the broth from P. florida and TERI-G1 cultures showed a broad-spectrum antibacterial activity against S. epidermidis, E. coli, M. luteus and B. megaterium. Ethyl acetate extracts of the broth from TERI-G3 showed the highest SPF activity of 34.02 at 200 µg. ml-1 concentration. Ethyl acetate extract of the broth from F. velutipes, P. florida, P. ostreatus, and TERI-G1 showed comparable antioxidant activity of 66.86%, 79.51%, 82.02%, and 69.58% respectively when compared to ascorbic acid (85.83%) and quercetin (83.09%) taken as positive control in the study and their total phenolic contents were found to be 6.93, 43.68, 20.88 and 13.77 Gallic acid equivalent (GAE) per gram. The minimal inhibitory concentration of F. velutipes, P. florida, P. ostreatus, and TERI-G1 was found to be 3552.89 µg. ml-1, 1250 µg. ml-1, 2418.9 µg. ml-1 and 3219 µg. ml-1 respectively. Conclusion: The work is in progress to identify and characterize TERI-G1and TERI G3 cultures. Further studies on the anti-inflammatory, anti-tyrosinase, elastase inhibition properties of the cultures will be assessed to identify potential cosmeceutical active ingredients with promising applications in cosmeceutical products.

scholarly journals Pengaruh Jenis Pelarut Terhadap Nilai Sun Protection Factor Maserat Daun Kelor

2019 ◽  
Vol 1 (1) ◽  
pp. 9-13
Author(s):  
Venny Diah Ningsih ◽  
Isma Oktadiana
Keyword(s):  
Antioxidant Activity ◽  
Experimental Research ◽  
Research Method ◽  
Descriptive Analysis ◽  
Sun Protection ◽  
Protection Factor ◽  
Analysis Design ◽  
Sun Protection Factor

Moringa leaves are one part of the plant which has many health properties one of which is an antioxidant. One of the content of Moringa leaves which contains antioxidant activity is quercetin. Quercetin Is polar and insoluble in air. An experimental research method with a descriptive analysis design, starting with making Moringa leaf maserat, screening, and testing the SPF value, using a UV-Vis spectrophotometer. The purpose of this research was to find out the type of solvent against the SPF value of Moringa leaf maserat. Based on the experiments that have been carried out the methanol (6.75) solvent elds a higher SPF value compared to ethanol (5.32).   Keyword: Moringa Leaf, Solvent, SPF. ABSTRAK   Daun kelor merupakan salah satu bagian tanaman yang memiki banyak khasiat untuk kesehatan salah satunya ialah sebagai antioksidan. Salah satu kandungan daun kelor yang memiliki aktivitas sebagai antioksidan aialah quercetin. Quercetin Bersifat polar dan tidak larut dalam air. Metode penelitian eksperimental dengan desain analisis deskriptif, dimulai dengan pembuatan maserat daun keor, penyaringan, dan pengujian Nilai SPF, menggunakan Spektrofotometer UV-Vis. Tujuan dari penelitian ini ialah untuk mengetahui pengaruh jenis pelarut terhadap nilai SPF maserat daun kelor. Berdasarkan percobaan yang telah dilakukan didaptkan hasil bahawa pelarut methanol (6,75) memberikan nilai SPF lebih tinggi dibandingkan dengan dengan pelarut etanol (5.32).   Kata Kunci : Daun Kelor, Pelarut, SPF

scholarly journals Sun protection factor, total phenol, flavonoid contents and antioxidant activity of medicinal plants from Iran

2021 ◽  
Vol 18 (7) ◽  
pp. 1443-1448
Author(s):  
Zahra Hashemi ◽  
Mohammad Ali Ebrahimzadeh ◽  
Masoumeh Khalili
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Sun Protection ◽  
Protection Factor ◽  
Artemisia Absinthium ◽  
Sun Protection Factor

Purpose: To evaluate the correlation between sun protection factor (SPF) and the content of phenol and flavonoid and antioxidant activity. Methods: Different parts of 9 medicinal plants were extracted with methanol using three extraction methods (percolation, Soxhlet and ultrasonically assisted extraction) to obtain 42 crude extracts. Their phenol and flavonoid contents, and antioxidant activities were determined using Folin-Ciocalteu reagent, aluminum chloride method and DPPH radical-scavenging activity, respectively. The SPF values were determined and correlated with the phenol and flavonoid contents as well as antioxidant activities. Results: The phenol and flavonoid contents, and antioxidant activities ranged from 54.16-688.97mg GAE/g, 13.38-146.60 mg QE/g and 9.5-1472.4 µg/mL, respectively while the SPFs were between 0.067 and 0.841. The highest SPF was related to Cucumis melo L. ultrasonically assisted leaf extract (0.841) and Artemisia absinthium L., aerial parts extracted with percolation method (0.717). A significant correlation was found between SPF and phenolic (p= 0.003) and flavonoid contents (p= 0.023). Conclusion: This study showed a correlation between SPF and phenolic and flavonoid contents. Ultrasonically assisted extract of C. melo leaf has suitable SPF and can be used in sun screen formulations.

scholarly journals Sunscreen Activities of Bark Artocarpus heterophyllus against Ultraviolet Ray (Sun Protection Factor) in Lotion Formula

2020 ◽  
Vol 8 (A) ◽  
pp. 461-467
Author(s):  
Bastian Arifin ◽  
Rosnani Nasution ◽  
Savila Savila ◽  
Rika Ramadani ◽  
Hira Helwati ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Ethanol Extract ◽  
Sun Protection ◽  
Negative Control ◽  
Gas Chromatography Mass Spectrometry ◽  
Hydroxyl Groups ◽  
Double Bonds ◽  
Protection Factor ◽  
Artocarpus Heterophyllus ◽  
Sun Protection Factor

Sunscreen lotion is used to prevent the skin from ultraviolet radiation, which can cause various diseases, such as acne, burning, redness, swelling, wrinkling, and skin cancer. Natural sunscreen lotions contain chemical compounds that have relatively long conjugated double bonds or the presence of hydroxyl groups. The result of the maceration of Artocarpus heterophyllus bark produced ethanol and methanol extracts, based on gas chromatography-mass spectrometry characterization, both extracts contained straight and cyclic chain compounds which had conjugated double bonds. The number of double-bonded compounds in ethanol extract is more than in methanol extract. Sun protection factor (SPF) value of ethanol extract lotion formula is greater than SPF value of methanol extract lotion formula, the SPF value ethanol extract lotion formula at concentration 2; 2.5; 5; 7.5; and 12% were consecutive: 29.801 ± 0.224; 32.797 ± 0.161; 33.808 ± 0.165; 34.580 ± 0.295; and 35.015 ± 0.169, (SPF value of negative control: 0.435 ± 0.2839 and positive control, 31 ± 0.0284). SPF value of methanol extract lotion formula for concentration 2; 2.5; 5; 7.5; and 12% was 14.407 ± 0.010; 26.549 ± 0.476; 30.274 ± 0.208; 32.031 ± 0.302; and 31.942 ± 0.324. Each extract SPF value is higher than the SPF value of the lotion formula. The result of testing the physical properties of the lotion formula shows that the lotion type is o/w, the lotion viscosity ranges from 2450 cP to 4780.0 cP meet Standar Nasional Indonesia (SNI) standards. The pH test results ranged from 6.020 to 7.845 meets SNI. The spreading power of the lotion ranges from 4.70 to 6.75 cm. The adhesive strength of the lotion ranges from 16 to 41 s.

The cosmetic potential of the medicinal halophyte Tamarix gallica L. (Tamaricaceae) growing in the eastern of Algeria: photoprotective and antioxidant activities

2020 ◽  
Vol 23 ◽  
Author(s):  
Mostefa Lefahal ◽  
El-Hani Makhloufi ◽  
Warda Trifa ◽  
Radia Ayad ◽  
Mohamed El Hattab ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Total Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Protection Factor ◽  
Total Antioxidant ◽  
Sun Protection Factor ◽  
Phenolic And Flavonoid ◽  
Tamarix Gallica

Aim and Objective: Currently, the use of ingredients from natural sources has gained great attention in cosmetic field especially for the development of new photoprotective formulations. Therefore, the present study aimed to evaluate the cosmetic potential of the methanolic crude extract and the ethyl acetate fraction of the medicinal halophyte Tamarix gallica (Tg) growing in the area of Tebessa in eastern of Algeria, by assessing their phenolic and flavonoid contents, photoprotective and antioxidant activities. Methods: The research approach consisted of determination phenolic and flavonoid contents of aerial parts via FolinCiocalteu and aluminum chloride methods respectively. The antioxidant activity was measured through two in vitro methods DPPH radical scavenging activity and total antioxidant capacity test (TAC). The in vitro photoprotective effect was performed according to the parameter SPF (Sun Protection Factor) by using UV spectroscopic method in UV-B region (290-320 nm). Results: The methanolic extract (Tg-MeOH) and ethyl acetate (Tg-EtOAc) fraction showed good antioxidant activity with IC50’s 14,05±0,66, 27,58±1,98 µg/mL respectively in DPPH test. Furthermore, both extracts displayed strong total antioxidant capacity (287.01±7.85, 246.7±1.12 mg AAE/g, respectively) in TAC test. Both extracts exhibited high photoprotective activity, with sun protection factor (SPF) values 37.03±0.22 and 36.08±0.03. The antioxidant and photoprotective activities of these extracts is probably related to polyphenols content (190.27±0.74 mg AGE /g and 121.77±1.29 mg AGE /g, respectively) , and flavonoids (78.75±0.06 mg QE /g and 58.67±1.19mg/g). Conclusion: Our finding show that extracts of Tamarix gallica L could be a promising source to be mixed as natural sunscreens and antioxidants agent into photoprotective cosmetic formulations.

scholarly journals The determination of antioxidants activity and sunblock Sterculia populifolia extract- based cream

2018 ◽  
Author(s):  
Nur Khairi ◽  
Suryani As’ad ◽  
Khairuddin Djawad ◽  
Gemini Alam
Keyword(s):  
Antioxidant Activity ◽  
Physical Stability ◽  
Sun Protection ◽  
Stability Parameter ◽  
Protection Factor ◽  
Antioxidants Activity ◽  
Ic50 Value ◽  
Sun Protection Factor

Sterculia populifolia is commonly used as the ingredient of traditional herb in Timor Island, East Nusa Tenggara, Indonesia. It contains flavonoid which roles as antioxidant. This flavonoid can be sunblock due to the existence of chromophore which commonly gives color to plants. This study aimed to determine the activity of antioxidants and sunblock through the value of Sun Protection Factor (SPF) from Sterculia populifolia extract-based cream and cream preparation extract. This cream was formulated into four formulas; extract concentrate 0% (F0), 1% (F1), 3% (F2) and 5% (F3). The result showed that the whole formula of cream preparation filled the physical stability parameter. F3 cream had IC50 79.179 ppm value while SPF had 5.6 value higher than F1 and F2. The smaller the IC50 value, the bigger antioxidant activity and SPF increased.

scholarly journals PENENTUAN NILAI SPF EKSTRAK ETIL ASETAT DAUN MANGGA GEDONG MENGGUNAKAN SPEKTROFOTOMETRI UV - VIS

2019 ◽  
Vol 1 (2) ◽  
pp. 157-165
Author(s):  
Nia Lisnawati ◽  
M Fathan N.U ◽  
Dwi Nurlitasari
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Negative Impact ◽  
Uv Light ◽  
Antioxidant Properties ◽  
Sun Protection ◽  
Flavonoid Compound ◽  
Protection Factor ◽  
Sun Protection Factor

One way that is done to reduce the negative impact of sunlight is by using sunscreen. Sunscreen can protect the skin by spreading sunlight or absorbing sunlight radiation energy that is about the skin. Phenolic compounds especially flavonoid groups have the potential as a sunscreen because of the chromophore group (single conjugated double bond) capable of absorbing UV light both UV A and UV B, thus reducing the intensity of the skin. Flavonoids have three properties of photoprotektor that is UV absorption, antioxidant properties, and modulate some DNA signaling pathways. The potential of sunscreen can be expressed with Sun Protection Factor (SPF). SPF is designated as UV protection B and is not specifically intended to resist UV A and UV C. The purpose of this research is to know the value of SPF (Sun Protection Factor) of ethyl acetate extract of mango gedong leaves using UV-Vis spectrophotometry. Simplicia gedong mango leaves were extracted using ethyl acetate solvent by reflux. Gedong mango leaf extract identified flavonoid compound using 10% NaOH and H2SO4 (concentrated). Determination of SPF (Sun Protection Factor) value of med gedong leaf extract using UV-Vis spectrophotometry method. The concentration of ethyl acetate extract of mango gedong leaves used was 120 ppm, 240 ppm and 360 ppm. The SPF (Sun Protection Factor) values were analyzed using Mansur method. The result of identification of flavonoid compound showed that the extract of ethyl acetate of mango gedong leaves positively contain flavonoids, while the result of SPF (Sun Protection Factor) extract of ethyl acetate of mango gedong leaves was 5,556; 16,675 and 22,243. The SPF (Sun Protection Factor) extract of 5,556 muddy ethyl acetate leaves including moderate protection type, 16,675 and 22,243 including ultra-protection type when tested using UV-Vis spectrophotometry by Mansur method

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