scholarly journals Lignocellulosic Waste Degradation Potential of Some Cellulolytic Fungal Strains Isolated from Putrid Fruits

2020 ◽  
Vol 14 (4) ◽  
pp. 2585-2593
Author(s):  
H.A. Akinyele ◽  
A.A.T. Taliat ◽  
G.C. Enwerem ◽  
O.G. Dawodu ◽  
O.S. Owojuyigbe
Keyword(s):  
Sugarcane Bagasse ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Lignocellulosic Waste ◽  
Corn Cob ◽  
Lignocellulosic Wastes ◽  
Scopulariopsis Brevicaulis ◽  
Waste Degradation ◽  
Pretreated Sugarcane Bagasse ◽  
Industrial Level

Fungi are plentiful in nature and they are found growing on wastes of wood materials. These wastes are equally found in our environment with no usefulness. The aim of this study was to exploit the probability of the isolated fungi from fruits to produce cellulase from wastes of lignocellulosic materials. Cellulase- producing fungi were isolated from fruits (tomato, banana, plantain). The organisms were screened for cellulase production. Culture conditions were optimized with pH, temperature and carbon. Cellulase was produced using lignocellulosic wastes; sawdust, corn cob, sugarcane bagasse. Six cellulase producers were isolated, four of which were selected for synthesis and quantification of the cellulase. The fungi were identified as Saccharomyces cerevisiae4, Trichoderma species1 and Scopulariopsis brevicaulis1. Of all the tested substrates used in this study, pretreated sugarcane bagasse at 3% w/v concentration with Scopulariopsis brevicaulis gave highest cellulase production 18.18 U/mL at 40°C, 5day incubation time and pH 5, followed by Trichoderma with 12.39 U/mL. These fungi are good potentials cellulase producers that can be considered at industrial level.

scholarly journals Cellulases by Penicillium sp. in different culture conditions

Bioethanol ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Leyanis Mesa ◽  
Carmen A. Salvador ◽  
Mónica Herrera ◽  
Daimí I. Carrazana ◽  
Erenio González
Keyword(s):  
Sugarcane Bagasse ◽  
Filter Paper ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Cellulolytic Enzymes ◽  
Solid Loading ◽  
Penicillium Sp ◽  
Factors Influencing ◽  
Cellulosic Substrate ◽  
Pretreated Sugarcane Bagasse

AbstractThe high cost of cellulolytic enzymes used in the ethanol production process has led to a growing interest in situ production. The evaluation of the influence of several factors in the fungus Penicillium sp. cellulase production using pretreated sugarcane bagasse is very interesting. Penicillium sp. cellulase production by using filter paper as cellulosic substrate and the use of glucose, sucrose and lactose like co-substrates was assessed. In the experiments using filter paper as a cellulosic substrate, the highest FPase enzyme activity obtained was 280 FPU.L-1 using sucrose as co-substrate. Subsequently, the study of pretreated sugarcane bagasse was conducted using Plackett-Burman experimental design with analysis of 6 factors influencing the process. The highest FPase activity was 615.1 FPU.L-1. The factors influencing FPase and β- glucosidase activity were the use of molasses and the solid loading. The successful use of molasses as co-substrate opens perspectives for future researches.

Use of Spectroscopic and Imaging Techniques to Evaluate Pretreated Sugarcane Bagasse as a Substrate for Cellulase Production Under Solid-State Fermentation

2013 ◽  
Vol 172 (5) ◽  
pp. 2348-2362 ◽  
Author(s):  
Ursula Fabiola Rodríguez-Zúñiga ◽  
Victor Bertucci Neto ◽  
Sonia Couri ◽  
Silvio Crestana ◽  
Cristiane Sanchez Farinas
Keyword(s):  
Solid State ◽  
Sugarcane Bagasse ◽  
Solid State Fermentation ◽  
Imaging Techniques ◽  
Cellulase Production ◽  
Pretreated Sugarcane Bagasse

Production and optimization of cellulase from agricultural waste and its application in bioethanol production by simultaneous saccharification and fermentation

2016 ◽  
Vol 27 (1) ◽  
pp. 22-35 ◽  
Author(s):  
Elsa Cherian ◽  
M. Dharmendira Kumar ◽  
G. Baskar
Keyword(s):  
Design Methodology ◽  
Simultaneous Saccharification And Fermentation ◽  
Agricultural Waste ◽  
Cellulase Production ◽  
Reducing Sugar ◽  
Bioethanol Production ◽  
Corn Cob ◽  
Content Type ◽  
Optimized Conditions ◽  
Simultaneous Saccharification

Purpose – The purpose of this paper is to optimize production of cellulase enzyme from agricultural waste by using Aspergillus fumigatus JCF. The study also aims at the production of bioethanol using cellulase and yeast. Design/methodology/approach – Cellulase production was carried out using modified Mandel’s medium. The optimization of the cellulase production was carried out using Plackett-Burman and Response surface methodology. Bioethanol production was carried out using simultaneous saccharification and fermentation. Findings – Maximum cellulase production at optimized conditions was found to be 2.08 IU/ml. Cellulase was used for the saccharification of three different feed stocks, i.e. sugar cane leaves, corn cob and water hyacinth. Highest amount of reducing sugar was released was 29.1 gm/l from sugarcane leaves. Sugarcane leaves produced maximum bioethanol concentration of 9.43 g/l out of the three substrates studied for bioethanol production. Originality/value – The present study reveals that by using the agricultural wastes, cellulase production can be economically increased thereby bioethanol production.

The influence of culture conditions on mycelial structure and cellulase production by Trichoderma reesei Rut C-30

2000 ◽  
Vol 26 (5-6) ◽  
pp. 394-401 ◽  
Author(s):  
F.C. Domingues ◽  
J.A. Queiroz ◽  
J.M.S. Cabral ◽  
L.P. Fonseca
Keyword(s):  
Trichoderma Reesei ◽  
Cellulase Production ◽  
Culture Conditions ◽  
C 30

Production of cellulases by solid state fermentation using natural and pretreated sugarcane bagasse with different fungi

2019 ◽  
Vol 17 ◽  
pp. 1-6 ◽  
Author(s):  
Gabriella Soares Borges Salomão ◽  
Jacyele Clarindo Agnezi ◽  
Larissa Bastos Paulino ◽  
Luana Borchardt Hencker ◽  
Taísa Shimosakai de Lira ◽  
...  
Keyword(s):  
Solid State ◽  
Sugarcane Bagasse ◽  
Solid State Fermentation ◽  
Pretreated Sugarcane Bagasse

scholarly journals On-site integrated production of cellulases and β-glucosidases by Trichoderma reesei Rut C30 using steam-pretreated sugarcane bagasse

2018 ◽  
Author(s):  
Marcella Fernandes de Souza ◽  
Elba Pinto da Silva Bon ◽  
Ayla Sant’ Ana da Silvab
Keyword(s):  
Carbon Source ◽  
Lignocellulosic Biomass ◽  
Trichoderma Reesei ◽  
Sugarcane Bagasse ◽  
Enzyme Preparation ◽  
Integrated Approach ◽  
Aspergillus Awamori ◽  
Pretreated Sugarcane Bagasse ◽  
Hydrolysis Of ◽  
Rut C30

AbstractThe high cost of commercial cellulases still hampers the economic competitiveness of the production of fuels and chemicals from lignocellulosic biomasses. This cost may be decreased by the on-site production of cellulases with the integrated use of the lignocellulosic biomass as carbon source. This integrated approach was evaluated in the present study whereby steam-pretreated sugarcane bagasse (SPSB) was used as carbon source for the production of cellulases by Trichoderma reesei Rut C30 and the produced enzymes were subsequently used for SPSB hydrolysis. An enzyme preparation with a high cellulase activity, of 1.93 FPU/mL, was obtained, and a significant β-glucosidase activity was achieved in buffered media, indicating the importance of pH control during enzyme production. The hydrolysis of SPSB with the laboratory-made mixture resulted in a glucose yield of 80%, which was equivalent to those observed for control experiments using commercial enzymes. Even though the supplementation of this mixture with external β-glucosidase from Aspergillus awamori was found to increase the initial hydrolysis rates, it had no impact on the final hydrolysis yield. It was shown that SPSB is a promising carbon source for the production of cellulases and β-glucosidases by T. reesei Rut C30 and that the enzyme preparation obtained is effective for the hydrolysis of SPSB, supporting the on-site integrated approach to decrease the cost of the enzymatic hydrolysis of lignocellulosic biomass.

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