Cellulases by Penicillium sp. in different culture conditions

AbstractThe high cost of cellulolytic enzymes used in the ethanol production process has led to a growing interest in situ production. The evaluation of the influence of several factors in the fungus Penicillium sp. cellulase production using pretreated sugarcane bagasse is very interesting. Penicillium sp. cellulase production by using filter paper as cellulosic substrate and the use of glucose, sucrose and lactose like co-substrates was assessed. In the experiments using filter paper as a cellulosic substrate, the highest FPase enzyme activity obtained was 280 FPU.L-1 using sucrose as co-substrate. Subsequently, the study of pretreated sugarcane bagasse was conducted using Plackett-Burman experimental design with analysis of 6 factors influencing the process. The highest FPase activity was 615.1 FPU.L-1. The factors influencing FPase and β- glucosidase activity were the use of molasses and the solid loading. The successful use of molasses as co-substrate opens perspectives for future researches.

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Lignocellulosic Waste Degradation Potential of Some Cellulolytic Fungal Strains Isolated from Putrid Fruits

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.14.4.34 ◽

2020 ◽

Vol 14 (4) ◽

pp. 2585-2593

Author(s):

H.A. Akinyele ◽

A.A.T. Taliat ◽

G.C. Enwerem ◽

O.G. Dawodu ◽

O.S. Owojuyigbe

Keyword(s):

Sugarcane Bagasse ◽

Cellulase Production ◽

Culture Conditions ◽

Lignocellulosic Waste ◽

Corn Cob ◽

Lignocellulosic Wastes ◽

Scopulariopsis Brevicaulis ◽

Waste Degradation ◽

Pretreated Sugarcane Bagasse ◽

Industrial Level

Fungi are plentiful in nature and they are found growing on wastes of wood materials. These wastes are equally found in our environment with no usefulness. The aim of this study was to exploit the probability of the isolated fungi from fruits to produce cellulase from wastes of lignocellulosic materials. Cellulase- producing fungi were isolated from fruits (tomato, banana, plantain). The organisms were screened for cellulase production. Culture conditions were optimized with pH, temperature and carbon. Cellulase was produced using lignocellulosic wastes; sawdust, corn cob, sugarcane bagasse. Six cellulase producers were isolated, four of which were selected for synthesis and quantification of the cellulase. The fungi were identified as Saccharomyces cerevisiae4, Trichoderma species1 and Scopulariopsis brevicaulis1. Of all the tested substrates used in this study, pretreated sugarcane bagasse at 3% w/v concentration with Scopulariopsis brevicaulis gave highest cellulase production 18.18 U/mL at 40°C, 5day incubation time and pH 5, followed by Trichoderma with 12.39 U/mL. These fungi are good potentials cellulase producers that can be considered at industrial level.

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Enhanced bioethanol production by fed-batch simultaneous saccharification and co-fermentation at high solid loading of Fenton reaction and sodium hydroxide sequentially pretreated sugarcane bagasse

Bioresource Technology ◽

10.1016/j.biortech.2017.01.028 ◽

2017 ◽

Vol 229 ◽

pp. 204-210 ◽

Cited By ~ 18

Author(s):

Teng Zhang ◽

Ming-Jun Zhu

Keyword(s):

Sodium Hydroxide ◽

Sugarcane Bagasse ◽

Fenton Reaction ◽

Solid Loading ◽

Bioethanol Production ◽

Fed Batch ◽

High Solid ◽

High Solid Loading ◽

Pretreated Sugarcane Bagasse ◽

Simultaneous Saccharification

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Fed-batch strategies for saccharification of pilot-scale mild-acid and alkali pretreated sugarcane bagasse: Effects of solid loading and surfactant addition

Industrial Crops and Products ◽

10.1016/j.indcrop.2018.04.026 ◽

2018 ◽

Vol 119 ◽

pp. 283-289 ◽

Cited By ~ 11

Author(s):

Absai da Conceição Gomes ◽

Danuza Nogueira Moysés ◽

Lidia Maria Melo Santa Anna ◽

Aline Machado de Castro

Keyword(s):

Sugarcane Bagasse ◽

Pilot Scale ◽

Solid Loading ◽

Fed Batch ◽

Pretreated Sugarcane Bagasse ◽

Mild Acid

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Using in-situ viscosimetry and morphogranulometry to explore hydrolysis mechanisms of filter paper and pretreated sugarcane bagasse under semi-dilute suspensions

Biochemical Engineering Journal ◽

10.1016/j.bej.2017.07.006 ◽

2017 ◽

Vol 127 ◽

pp. 9-20 ◽

Cited By ~ 4

Author(s):

Tuan Le ◽

Dominique Anne-Archard ◽

Véronique Coma ◽

Xavier Cameleyre ◽

Eric Lombard ◽

...

Keyword(s):

Sugarcane Bagasse ◽

Filter Paper ◽

Dilute Suspensions ◽

Pretreated Sugarcane Bagasse

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Use of Spectroscopic and Imaging Techniques to Evaluate Pretreated Sugarcane Bagasse as a Substrate for Cellulase Production Under Solid-State Fermentation

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-013-0678-0 ◽

2013 ◽

Vol 172 (5) ◽

pp. 2348-2362 ◽

Cited By ~ 19

Author(s):

Ursula Fabiola Rodríguez-Zúñiga ◽

Victor Bertucci Neto ◽

Sonia Couri ◽

Silvio Crestana ◽

Cristiane Sanchez Farinas

Keyword(s):

Solid State ◽

Sugarcane Bagasse ◽

Solid State Fermentation ◽

Imaging Techniques ◽

Cellulase Production ◽

Pretreated Sugarcane Bagasse

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Conclusive selection of optimal parameters for cellulase production by Talaromyces verruculosus IIPC 324 under SSF via saccharification of acid-pretreated sugarcane bagasse

Biofuels ◽

10.1080/17597269.2018.1449063 ◽

2018 ◽

Vol 12 (1) ◽

pp. 61-69 ◽

Cited By ~ 1

Author(s):

Lavika Jain ◽

Akhilesh Kumar Kurmi ◽

Deepti Agrawal

Keyword(s):

Sugarcane Bagasse ◽

Cellulase Production ◽

Optimal Parameters ◽

Pretreated Sugarcane Bagasse ◽

Selection Of

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Cellulase Production from Filamentous Fungi for Its Application in the Hydrolysis of Wheat Straw

MRS Proceedings ◽

10.1557/opl.2015.134 ◽

2015 ◽

Vol 1763 ◽

Cited By ~ 1

Author(s):

L. Toscano-Palomar ◽

G. Montero-Alpirez ◽

M. Stilianova-Stoytcheva ◽

E. Vertiz-Pelaez ◽

y E. Romero Uscanga

Keyword(s):

Aspergillus Niger ◽

Wheat Straw ◽

Filamentous Fungi ◽

Submerged Fermentation ◽

Carbon Sources ◽

Nitrogen Sources ◽

Cellulase Production ◽

Culture Conditions ◽

Mineral Medium ◽

Cellulolytic Enzymes

ABSTRACTExtended research has been developed in the use of wheat straw (WS) as biomass for the production of biofuels (bioethanol), including the processes of degradation of cellulose by enzymatic systems. For centuries, Cellulose has been used by man; however, its enormous potential as a renewable energy source was recognized only after the discovery of cellulose degrading enzymes (cellulases). A wide variety of microorganisms can produce cellulolytic enzymes under appropriate culture conditions and among these microorganisms are filamentous fungi of the genera Trichoderma, Aspergillus, Penicillium and Fusarium. The purpose of this study was to produce cellulase enzyme from previously isolated and characterized filamentous fungi. Cellulytic fungi belonged toAspergillus flavus, Aspergillus niger, Aspergillus oryzae, Penicillium chrysogenum, Penicillium sp.,andTrichoderma harzianum.All these strains were preserved by lyophilization and also kept in sterile media (sand and soil) at 4 °C. The production of cellulases by submerged fermentation was performed in a Mandels mineral medium. The nitrogen sources were urea and ammonium sulfate. Glucose alone was used in the pre-inoculum, and dried and ground wheat straw was used in the fermentation as carbon sources. Subcultures of spore suspensions were incubated with orbital stirring (120 rpm) at 30 °C for 48 hours and used as inoculum for submerged fermentation with wheat straw as substrate in mineral medium with an initial pH of 5. Activity cellulase was determined by the method of 3,5-dinitrosalicylic acid (DNS). The results showed that wheat straw have potential for use as a substrate in the production of cellulases.Aspergillus nigershowed the highest enzymatic activity from the cellulase produced 0.051 FPU (filter paper units) after 96 hours of fermentation.

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A novel recyclable furoic acid-assisted pretreatment for sugarcane bagasse biorefinery in co-production of xylooligosaccharides and glucose

Biotechnology for Biofuels ◽

10.1186/s13068-021-01884-3 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Lin Dai ◽

Tian Huang ◽

Kankan Jiang ◽

Xin Zhou ◽

Yong Xu

Keyword(s):

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Production Cost ◽

Solid Loading ◽

Fed Batch ◽

Acid Pretreatment ◽

Furoic Acid ◽

Cooling Crystallization ◽

Mineral Acids ◽

Hydrolysis Of

Abstract Background Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 °C for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization. Conclusion This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.

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