Correlation of serological markers and thrombocytopenia in early diagnosis of dengue infection

Background: Dengue is an acute viral infection with fatal complications. To reduce the disease burden, early and specific diagnosis of dengue hemorrhagic fever or dengue shock syndrome with supportive therapy reduces morbidity and mortality. This study was undertaken to evaluate the efficacy of NS1 antigen and platelet count.Methods: A total of 200 serum samples were collected from clinically suspected cases of dengue fever. Serum samples were tested for NS1, IgM and IgG and correlated with the platelet count.Results: Out of 200 samples screened in our study, 100 (50%) were positive for dengue parameters, 100 (50%) were negative. Out of 100 positive cases, 95 (95%) cases were positive for NS1antigen either alone or in combination with antibodies. 84 (84%) cases were exclusively positive for NS1 antigen only. Primary infection (positive for NS1 Ag, IgM, NS1 + IgM) was seen in 99 (99%) cases and secondary infection (positive for IgG, NS1 + IgG, IgM + IgG, NS1+ IgM+ IgG) was seen in 1 case (1%). Out of 100 positive cases, thrombocytopenia was observed in 60 (60%) of patients while 40 (40%) had platelet count within normal range.Conclusions: Dengue is endemic to Indian subcontinent. Currently no specific antiviral therapy is available. Measuring platelet count and detecting the NS1 antigen plays a vital role in early diagnosis, management and implementing the control measures in community to avoid spread of dengue.

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Seroprevalence of Dengue Infection in a Tertiary Care Hospital in Assam

International Journal of Medical and Dental Sciences ◽

10.18311/ijmds/2018/18905 ◽

2018 ◽

Vol 7 (1) ◽

pp. 1582 ◽

Cited By ~ 1

Author(s):

Liza Goswami ◽

Chowdhury Runumi ◽

Elmy S. Rasul

Keyword(s):

Dengue Infection ◽

Tertiary Care ◽

Age Groups ◽

Hemorrhagic Fever ◽

Health Concern ◽

Care Hospital ◽

Serum Samples ◽

Igm Antibody ◽

Ns1 Antigen ◽

Antibody Elisa

Background: Dengue fever (DF) and dengue hemorrhagic fever (DHF) are important arthropod borne viral diseases. Dengue in India has dramatically expanded over the last few decades, with rapidly changing epidemiology. Dengue is emerging as major public health concern in northeast India and spreading with increased morbidity.Objective:This study was carried out to determine the seroprevalence of Dengue infection among patients attending in Fakhruddin Ali Ahmed Medical College and Hospital, Barpeta, Assam during the period 2013-2016.Methods: A retrospective study was done from the year 2013 to 2016. A total of 340 serum samples received in the department of microbiology FAAMCH, were tested for the confirmation of suspected cases of dengue. Dengue NS1 antigen and Dengue IgM antibody ELISA tests were performed for the confirmation of dengue cases. We estimated the incidence by applying age, sex and season adjusted dengue positivity.Results: Out of 340 samples tested, 68 were positive either by NS1 antigen or for IgM antibody ELISA tests. These comprised all age groups of both sexes with higher incidence of cases in young males aged 26– 60 years.Conclusion: From this study, it is apparent that dengue surveillance and control should be enhanced by wider use of laboratory testing to confirm dengue as a cause of fever of unknown origin, especially during the local dengue transmission season.

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Burden of Dengue and Chikungunya – A Retrospective Study

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.15.2.30 ◽

2021 ◽

Author(s):

J.V. Sathish ◽

Mita D. Wadekar ◽

S. Jayashree ◽

C. Pooja

Keyword(s):

Retrospective Study ◽

Early Diagnosis ◽

Dengue Infection ◽

Control Measures ◽

Enzyme Linked Immunosorbent Assay ◽

Age Group ◽

Capture Elisa ◽

Igm Antibody ◽

Ns1 Antigen ◽

And Control

Arboviral infections like dengue fever and chikungunya are the most common infections that share the same Aedes mosquito vectors. Clinical presentations of these two infections are also similar, especially in initial stages. Non-structural antigen (NS1 Ag)detection for dengue and detection of IgM antibodies by capture ELISA for chikungunya and dengue infection may help in the early diagnosis. Early diagnosis is essential for the treatment and control measures. The present study was conducted to know the burden of dengue and chikungunya. A retrospective study was conducted for a period of 1 year from Dec 2017 to Nov 2018 to know the burden of dengue and chikungunya in Chamarajanagar. Dengue (> 5 days fever) and chikungunya testing was done by IgM antibody capture ELISA kits produced by NIV. Dengue samples (< 5 days fever) were subjected to NS1 antigen detection by microwell enzyme-linked immunosorbent assay (ELISA) from Qualpro diagnostics. The tests were carried out following manufacturer’s instruction. Samples received for dengue NS1 Ag testing was 446, of which, 49(11.0%) were positive and of 730 samples received for IgM antibody, 53 (7.3%) were positive. Age group commonly affected was 0-20 years 44(43.1%). Of 668 samples received for chikungunya test, 86 (12.9%) were positive. Maximum number of cases was seen in age group of 21-40 years 45(52.3%). Males 56(54.9%) were affected higher than female 46(45.1%) in dengue infection while in chikungunya, females 45(52.3%) were more affected than males 41(47.7%). Both infections are high in the month of June and July. Early detection of dengue by NS1 antigen and detection of Ig M antibodies by capture ELISA chikungunya and dengue infection helps in appropriate treatment and initiation of prevention and control measures by community awareness and vector control.

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THE STUDY OF DETECTION OF DENGUE CASES BY NS1 ANTIGEN AND IGM ANTIBODY IN RIMS, ADILABAD, INDIA.

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v3i11.720 ◽

2019 ◽

Vol 3 (11) ◽

Author(s):

Dr. Tanajee Zade ◽

Dr. K. Srinivas ◽

Dr. Akshay Berad

Keyword(s):

Early Diagnosis ◽

Dengue Fever ◽

Dengue Infection ◽

Tertiary Care ◽

High Morbidity ◽

Care Hospital ◽

Serum Samples ◽

Igg Antibody ◽

Igm Antibody ◽

Ns1 Antigen

Dengue fever is an acute febrile arboviral disease affecting tropical & subtropical regions of the world. Dengue infection produces a spectrum of clinical illness, ranging from an asymptomatic to its most severe form like dengue haemorrhagic fever and dengue shock syndrome. In view of high morbidity and mortality, it is imperative to have a rapid and sensitive laboratory assay for early detection of the dengue infection. The newer parameter NS1 antigen has gained a lot of interest for early diagnosis of the disease. Detection of non-structural antigen (NS1 Ag), IgM and IgG antibody may help in the early diagnosis. The present study was conducted in a RIMS Adilabad, tertiary care hospital & medical college in the Department of General Medicine. A total of 100 serum samples were processed from suspected cases of dengue fever by using dengue test for detection of NS1 antigen and IgG antibodies. Platelet counts of all these cases were noted. . Of these 100 subjects 85 were serologically proved to have dengue illness, 57 patients were NS1 antigen positive, 28 patients were IgM antibody positive patients. As the NS1 antigen is detectable in blood from day one after onset of fever, its assay is an effective tool for early diagnosis of dengue infection so as to avoid complications. Key words: Dengue, NS1 Antigen, IgM antibody, Platelet

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A PROSPECTIVE STUDY ON VARIOUS SEROLOGICAL MARKERS AND PLATELET COUNT IN DENGUE INFECTION IN AND AROUND KANCHEEPURAM.

10.36106/ijar/6001884 ◽

2021 ◽

pp. 45-46

Author(s):

Hamsadwani K P ◽

Sneka P ◽

Sangamithra V

Keyword(s):

Platelet Count ◽

Dengue Infection ◽

Febrile Illness ◽

Antibody Detection ◽

Serological Markers ◽

Serum Samples ◽

Platelet Counts ◽

Acute Viral Infection ◽

Ns1 Antigen ◽

A Prospective Study

INTRODUCTION: Dengue is an acute viral infection with potential fatal complications, presenting with non-specic fever that mimics other febrile illness. Specic antibody detection has been the mainstay of diagnosis which is prone for false positive and negative reactions. The newer parameter NS1 appears to be highly specic and reliable for diagnosis. Therefore, we tried to evaluate the association of platelet counts against NS1 and IgM / IgG in dengue infections. OBJECTIVE: To study the association of thrombocytopenia with NS1 antigen and specic antibodies in dengue infection in kancheepuram population. MATERIALS AND METHODS: Serum samples from clinically suspected dengue cases were tested for NS1,IgM,IgG by immunochromatography – based test. Platelet counts were obtained from all positive cases. Test results of dengue-specic parameters and platelet counts were compared. RESULTS: Of the 612 samples tested, 150 were positive for one or more dengue serological markers. Of the 150, 27(18%) were positive for NS1only, 45(30%)were positive for IgM, 54(36%) were positive for IgG only. Of the 150, 39(26%) showed platelet count less than 1 lakh. CONCLUSION: Inclusion of NS1 in the diagnosis of dengue increases the detection and specicity rate. Thus correlation of platelet count, NS1,IgM and ,IgG gives detailed picture of the stage and prognosis of the disease

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FALSE-NEGATIVE DENGUE CASES IN RORAIMA, BRAZIL: AN APPROACH REGARDING THE HIGH NUMBER OF NEGATIVE RESULTS BY NS1 AG KITS

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652014000500014 ◽

2014 ◽

Vol 56 (5) ◽

pp. 447-450 ◽

Cited By ~ 7

Author(s):

Pablo O. A. Acosta ◽

Fabiana Granja ◽

Cátia A. Meneses ◽

Ismael A. S. Nascimento ◽

Débora D. Sousa ◽

...

Keyword(s):

Health Care Providers ◽

Secondary Infection ◽

Dengue Infection ◽

False Negative ◽

Laboratory Data ◽

Detection Methods ◽

Lower Sensitivity ◽

Care Providers ◽

Qrt Pcr ◽

Ns1 Antigen

Serum samples from 150 NS1-negative (Platelia ELISA) patients presumptively diagnosed with dengue were analyzed by the TaqMan probed real-time reverse transcription PCR (TaqMan qRT-PCR) method. The qRT-PCR positive samples were tested for serotype by semi-nested RT-PCR and a qualitative immunochromatographic assay for IgG and IgM. Molecular detection methods showed 33 (22%) positive samples out of 150 NS1-antigen negative samples. Of these, 72% were collected up to day 2 after the onset of symptoms, when diagnostic sensitivity of NS1-antigen test assays is significantly enhanced. Most of the cases were not characterized as secondary infection. Twenty-eight samples were successfully serotyped, 75% of which for DENV-4, 14% for DENV-2, 7% for DENV-3 and 4% for DENV-1. These findings reaffirm the hyperendemic situation of the state of Roraima and suggest a lower sensitivity of the NS1 test, mainly when DENV-4 is the predominant serotype. Health care providers should therefore be aware of samples tested negative by NS1 antigen assays, especially when clinical symptoms and other laboratory data results show evidence of dengue infection.

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NS1 antigen: A new beam of light in the early diagnosis of dengue infection

Asian Pacific Journal of Tropical Medicine ◽

10.1016/j.apjtm.2016.10.007 ◽

2016 ◽

Vol 9 (12) ◽

pp. 1212-1214 ◽

Cited By ~ 2

Author(s):

Muhammad Suleman ◽

Rani Faryal ◽

Muhammad Masroor Alam ◽

Salmaan Sharif ◽

Shahzad Shaukat ◽

...

Keyword(s):

Early Diagnosis ◽

Dengue Infection ◽

Ns1 Antigen ◽

Antigen A

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Maiden outbreaks of dengue virus 1 genotype III in rural central India

Epidemiology and Infection ◽

10.1017/s0950268814000612 ◽

2014 ◽

Vol 143 (2) ◽

pp. 412-418 ◽

Cited By ~ 8

Author(s):

P. V. BARDE ◽

B. K. KORI ◽

M. K. SHUKLA ◽

P. K. BHARTI ◽

G. CHAND ◽

...

Keyword(s):

Dengue Virus ◽

Phylogenetic Analyses ◽

Dengue Infection ◽

Central India ◽

Febrile Illness ◽

Control Measures ◽

Serum Samples ◽

Serological Tests ◽

Genotype Iii ◽

Virus Serotype

SUMMARYDengue is regarded as the most important arboviral disease. Although sporadic cases have been reported, serotypes responsible for outbreaks have not been identified from central India over the last 20 years. We investigated two outbreaks of febrile illness, in August and November 2012, from Korea district (Chhattisgarh) and Narsinghpur district (Madhya Pradesh), respectively. Fever and entomological surveys were conducted in the affected regions. Molecular and serological tests were conducted on collected serum samples. Dengue-specific amplicons were sequenced and phylogenetic analyses were performed. In Korea and Narsinghpur districts 37·3% and 59% of cases were positive, respectively, for dengue infection, with adults being the worst affected. RT–PCR confirmed dengue virus serotype 1 genotype III as the aetiology. Ninety-six percent of infections were primary. This is the first time that dengue virus 1 outbreaks have been documented from central India. Introduction of the virus into the population and a conducive mosquitogenic environment favouring increased vector density caused the outbreak. Timely diagnosis and strengthening vector control measures are essential to avoid future outbreaks.

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Comparison of NS1 Antigen Detection by Rapid Immunochromatography Test and ELISA for Early Diagnosis of Dengue at a Government General Hospital, Ananthapuramu

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i57b34069 ◽

2021 ◽

pp. 378-381

Author(s):

Adireddi Paradesi Naidu ◽

Chitralekha Saikumar ◽

G. Sumathi ◽

Kalavathy Victor ◽

N. S. Muthiah

Keyword(s):

Rapid Test ◽

Dengue Shock Syndrome ◽

Hemorrhagic Fever ◽

Clinical History ◽

Elisa Test ◽

Microbiology Laboratory ◽

Serum Samples ◽

Test Sensitivity ◽

Ns1 Antigen ◽

History Of

Background: The incidence of Dengue hemorrhagic fever, Dengue shock syndrome associated with Dengue can be reduced by diagnosing Dengue early and by initiating early treatment to Dengue patients. This study was conducted to compare results of NS1 antigen rapid test and ELISA in clinically suspected dengue patients. Materials and Methods: Present study was a comparative study conducted on 100 Patients presented with clinical history of Dengue. At Microbiology Laboratory, serum of all samples was assessed for NS1 detection using antigen Rapid test and ELISA. Sensitivity & specificity values were calculated for NS1 antigen rapid test, compared with ELISA. Results: Out of 100 serum samples collected from suspected cases of Dengue in and around Anantapuramu, 30 (30%) were positive for ELISA and 28 (28%) were positive for Rapid diagnostic test. Sensitivity & specificity when only NS1 was considered on rapid test kits when compared with ELISA were 93.33%, 98.57%, Conclusion: It is concluded that ELISA test was superior in the diagnosis of Dengue and recommended on improvement in sensitivity of RDTs.

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Utility of rapid diagnostic kit tests for diagnosis of suspected dengue fever

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20191656 ◽

2019 ◽

Vol 7 (5) ◽

pp. 1670

Author(s):

Sarita Otta ◽

Bichitrananda Swain

Keyword(s):

Platelet Count ◽

Dengue Fever ◽

Rapid Test ◽

Febrile Illness ◽

Serum Samples ◽

Serological Tests ◽

Ns1 Antigen ◽

Elisa Technique ◽

Low Platelet Count ◽

Sensitivity Specificity

Background: Dengue fever often presents as an undifferentiated febrile illness requiring a laboratory test for identification. Serological tests particularly on rapid kits for the detection of NS1Antigen, IgG and IgM antibodies are the most commonly performed test across the country.Methods: The serum samples of suspected dengue cases were tested by Rapid test kits for assessing all the three parameters as well as by ELISA for NS1 antigen test. The platelet count of the patients was obtained from automated coulter counter. The results thus obtained were analyzed in Excel format.Results: The serum samples from 304 suspected Dengue fever cases were received in the lab, of which 190 samples were positive either by rapid or ELISA and 176 when rapid card test was considered alone Highest seropositivity of dengue cases were observed in the age group of ≥60 years (79.2%) followed by 45-59 years (70.7%). On rapid test, 78 cases were NS1 antigen positive of which 60 cases were positive only for NS1 antigen. When NS1 rapid and ELISA tests when compared, 16 kit negative tests were positive on ELISA while 34 kit positive tests were ELISA negative. Sensitivity, specificity, PPV and NPV when only NS1 was considered on rapid test kits when compared with ELISA were 78.9%, 87.8%, 63.8% and 93.8%. 33.5% of serologically positive cases of Dengue had low platelet count on admission while only among negative cases 17.2% had a low platelet.Conclusions: Rapid kits often show variable results thus needing a validation of them from end user. As a positive dengue test result is an essential prerequisite for diagnosis thus it is essential that for serological tests ELISA technique should be used for reporting. Thus, it also mandates more efforts at decentralization of NVBDCP to include both government and non government institutions.

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STUDY OF NS1 ANTIGEN DETECTION AND ASSOCIATION OF PLATELET COUNT WITH VARIOUS DENGUE SEROLOGICAL MARKERS IN DENGUE INFECTION: AN INSTITUTIONAL EXPERIENCE"

SRMS Journal of Medical Science ◽

10.21761/jms.v1i2.7357 ◽

2016 ◽

Vol 1 (2) ◽

Author(s):

Neelam Gupta ◽

Rahul Kumar Goyal

Keyword(s):

Platelet Count ◽

Dengue Infection ◽

Dengue Shock Syndrome ◽

Accurate Diagnosis ◽

Serological Markers ◽

Ns1 Protein ◽

New Approach ◽

Ns1 Antigen ◽

Institutional Experience ◽

Initiation Of Therapy

Introduction: Dengue is a mosquito-borne disease caused by dengue virus. Annually, it affects up millions of people worldwide. An early and accurate diagnosis of dengue in the acute phase of illness is important for identifying an epidemic and for initiation of therapy. Detection of the secreted NS1 protein is a new approach that aid in the early diagnosis. Platelet count is the only non-dengue parameter that can support the diagnosis of the dengue shock syndrome and dengue. This study was done to detect dengue parameters and correlate them with the platelet count. Material and Methods: This study was conducted from September 2016 to November 2016. Total 205 blood samples were collected from clinically suspected dengue cases. Serum was separated and tested for NS1 antigen, IgM and IgG using the immunochromatography kit. The platelet count was also recorded in all samples. Results: Out of 205 samples tested total 92(44.9%) specimens were positive for one or more dengue parameters. Among 92 specimens 45(49%) were positive for only NS1 antigen and 20 were positive for IgM only. Thrombocytopenia was recorded in 73(79%)out of 92 dengue positive cases. Conclusion: Inclusion of NS1 in the diagnosis of dengue increases the detection rate significantly in early phase of the infection.

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