scholarly journals Identification of Arbuscular Mychorizae Fungi on Oil Palm in Bireuen, Aceh

2021 ◽  
Vol 5 (2) ◽  
pp. 114-121
Author(s):  
Zaitun Ritaqwin Rita ◽  
Mizan Maulana ◽  
Nazalia
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Oil Palm ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Culture Method ◽  
Soil Samples ◽  
Soil Biology ◽  
National University ◽  
Maize Plants ◽  
Very High

Abstract   Oil palm plantations in Aceh , especially in Bireuen, are generally planted on red-yellow podzolic soil types. Arbuscular mycorrhizal fungi (AMF) are fungi who are symbiotically associated with 97% of plant species. This study aims to identify the type and population of mycorrhizal spores from oil palm rhizosphere soil samples using culture trapping techniques (maize, sorghum, kudzu). This research was carried out in a greenhouse and Experimental Field, Faculty of Agriculture, Islamic National University of Indonesia, Bireuen, Aceh. Isolation, identification and observation of AMF colonization on plant roots was carried out at the Laboratory of Soil Biology, Faculty of Agriculture, Syiah Kuala University. The parameters included the number of arbuscular mycorrhizal fungi spores and the percentage of root colonization using the trapping culture method. The trapping culture method used 50 g of soil samples on oil palm rhizosphere which aged 5 months, 7 years, 9 years and 12 years. The results showed that the most dominant mycorrhizae found was the Glomus type. The mycorrhizae spores types found in the trapping culture were Glomus, Acaulospora and Gigaspora. The host plant that produced the most AMF spores was sorghum, while the highest AMF colonization of the three host plants was found in the roots of maize plants (78%) with very high criteria. Keywords: Oil palm, Culture trapping, AMF  

scholarly journals Arbuscular Mycorrhizal Fungi Associated with Wati (Piper methysticum), a Medicinal Plant from Merauke Lowland, Papua, Indonesia

2018 ◽  
Vol 10 (2) ◽  
pp. 260-266
Author(s):  
Suharno Suharno ◽  
Supeni Sufaati ◽  
Verena Agustini ◽  
Rosye H. R. Tanjung
Keyword(s):  
Medicinal Plant ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Culture Method ◽  
Soil Samples ◽  
Local People ◽  
Piper Methysticum ◽  
Root Sample ◽  
Tract Infections

Indonesia is rich of natural resources, including the diversity of agricultural crops and other valuable plants. Many plant species used by local people for traditional herbal medicine and some other species used by drug industries. Among these, Wati (Piper methysticum, Piperaceae) is one of the medicinal plant found in Merauke lowland, Papua. It has been cultivated by local people because of it high value as medicinal and cultural uses. Wati plant is used to treat anti-stress, rheumatism, respiratory tract infections, tuberculosis, gonorrhea, headache etc. The habitat, including the microorganism in the soil plays an important role in the growth of this plant. Therefore, this study was conducted to identify the arbuscular mycorrhizal fungi (AMF) associated with the rhizosphere of Wati from Merauke lowland. Soil and root samples were collected from different locations and the colonization percentage on the root sample were determined. Our results showed that the number of the spores in the soil samples was 45-89 spores/50 g soil, while the colonization percentage on the root was 38.46-83.3%. Among 13 AMF morphospecies that found on the soil samples, 10 were identified to genus level such as Glomus, Clariodeoglomus, Acaulospora and Scutellospora, while the other were unidentified. Further work will be needed such as trap-plant culture method to get more information on the diversity of AMF associated with Wati.

scholarly journals The methylome of the model arbuscular mycorrhizal fungus, Rhizophagus irregularis, shares characteristics with early diverging fungi and Dikarya

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Anurag Chaturvedi ◽  
Joaquim Cruz Corella ◽  
Chanz Robbins ◽  
Anita Loha ◽  
Laure Menin ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Plant Species ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Rhizophagus Irregularis ◽  
Functional Differences ◽  
Phosphate Regulation ◽  
Very High

AbstractEarly-diverging fungi (EDF) are distinct from Dikarya and other eukaryotes, exhibiting high N6-methyldeoxyadenine (6mA) contents, rather than 5-methylcytosine (5mC). As plants transitioned to land the EDF sub-phylum, arbuscular mycorrhizal fungi (AMF; Glomeromycotina) evolved a symbiotic lifestyle with 80% of plant species worldwide. Here we show that these fungi exhibit 5mC and 6mA methylation characteristics that jointly set them apart from other fungi. The model AMF, R. irregularis, evolved very high levels of 5mC and greatly reduced levels of 6mA. However, unlike the Dikarya, 6mA in AMF occurs at symmetrical ApT motifs in genes and is associated with their transcription. 6mA is heterogeneously distributed among nuclei in these coenocytic fungi suggesting functional differences among nuclei. While far fewer genes are regulated by 6mA in the AMF genome than in EDF, most strikingly, 6mA methylation has been specifically retained in genes implicated in components of phosphate regulation; the quintessential hallmark defining this globally important symbiosis.

scholarly journals Isolation and identification of indigenous Arbuscular Mycorrhizal Fungi (AMF) of forest clove rhizosphere from Maluku, Indonesia

2021 ◽  
Vol 22 (8) ◽  
Author(s):  
Asri Subkhan Mahulette ◽  
Anggra Alfian ◽  
ABDUL KARIM KILKODA ◽  
IMELDA JEANETTE LAWALATA ◽  
DESSY ARIYANI MARASABESSY ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Spore Density ◽  
Wild Type ◽  
Isolation And Identification ◽  
Spore Count ◽  
Spore Number ◽  
Total Spore Count

Abstract. Mahulette AS, Alfian A, Kilkoda KA, Lawalata IJ, Marasabessy DA, Tanasale VL, Makaruku MH. 2021. Isolation and identification of indigenous Arbuscular Mycorrhizal Fungi (AMF) of forest clove rhizosphere from Maluku, Indonesia. Biodiversitas 22: 3613-3619. Forest clove is classified as wild-type and endemic to the Maluku (Moluccas) Islands, Indonesia. The different condition of growing areas causes various types of Arbuscular Mycorrhizal Fungi (AMF) associated with forest clove. The study aimed to identify and obtain indigenous AMF inoculums from the forest clove rhizosphere from two distribution areas in Maluku. The results of AMF identification found two types of spores from the genus Glomus in the rhizosphere of forest cloves from Ambon Island with a spore density of 35/50 g of soil. In comparison, three spores were found in Seram Island, two from the genus Scutellospora and one from the Acaulospora. With an overall spore density of 5/50 g of soil. After culture trapping, there was a change in type and an increase in spore density in soil samples from the rhizosphere of the two forest clove distribution areas. Soil samples from Ambon after trapping culture obtained two new types of spores from the genus Acaulospora with a total spore number of 57/50 g soil while in soil samples from Seram found three new types of spores from the genus Glomus with a total spore count of 104/50 g of soil.

scholarly journals Diversity of arbuscular mycorrhizal fungi (amf) in three agroecosystems with grass colosoana (Bothriochloa pertusa (L) A. Camus) in the department of Sucre

2016 ◽  
Vol 8 (1) ◽  
pp. 72
Author(s):  
FREDIS ESPITIA D ◽  
ALEXANDER PEREZ C
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Compacted Soils ◽  
San Marcos

The present study evaluated the diversity of arbuscular mycorrhizal fungi (AMF) associated soils from three ecosystems grown with pasture colosoana in three localities of the Department of Sucre, Colombia. Soil samples were collected, AMF spores isolated, count of spore was made and morphotypes isolated were classification at the level of species or genera. We found that the genus of AMF over prevailing in native and compacted soils in the three selected municipalities is the genus Glomus, followed by other genera to a lesser extent. In general the soils that presented greater wealth of genres of HMA were those of the municipality of San Marcos, while the lowest richness was found in the municipality of Sincelejo.

scholarly journals Impact of Land Use Intensity on the Species Diversity of Arbuscular Mycorrhizal Fungi in Agroecosystems of Central Europe

2003 ◽  
Vol 69 (5) ◽  
pp. 2816-2824 ◽  
Author(s):  
Fritz Oehl ◽  
Ewald Sieverding ◽  
Kurt Ineichen ◽  
Paul Mäder ◽  
Thomas Boller ◽  
...  
Keyword(s):  
Land Use ◽  
Arbuscular Mycorrhizal Fungi ◽  
Crop Rotation ◽  
Mycorrhizal Fungi ◽  
Root Colonization ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Land Use Intensity ◽  
High Input ◽  
Continuous Maize

ABSTRACT The impact of land use intensity on the diversity of arbuscular mycorrhizal fungi (AMF) was investigated at eight sites in the “three-country corner” of France, Germany, and Switzerland. Three sites were low-input, species-rich grasslands. Two sites represented low- to moderate-input farming with a 7-year crop rotation, and three sites represented high-input continuous maize monocropping. Representative soil samples were taken, and the AMF spores present were morphologically identified and counted. The same soil samples also served as inocula for “AMF trap cultures” with Plantago lanceolata, Trifolium pratense, and Lolium perenne. These trap cultures were established in pots in a greenhouse, and AMF root colonization and spore formation were monitored over 8 months. For the field samples, the numbers of AMF spores and species were highest in the grasslands, lower in the low- and moderate-input arable lands, and lowest in the lands with intensive continuous maize monocropping. Some AMF species occurred at all sites (“generalists”); most of them were prevalent in the intensively managed arable lands. Many other species, particularly those forming sporocarps, appeared to be specialists for grasslands. Only a few species were specialized on the arable lands with crop rotation, and only one species was restricted to the high-input maize sites. In the trap culture experiment, the rate of root colonization by AMF was highest with inocula from the permanent grasslands and lowest with those from the high-input monocropping sites. In contrast, AMF spore formation was slowest with the former inocula and fastest with the latter inocula. In conclusion, the increased land use intensity was correlated with a decrease in AMF species richness and with a preferential selection of species that colonized roots slowly but formed spores rapidly.

scholarly journals EKSPLORASI FUNGI MIKORIZA ARBUSKULA (FMA) PADA RIZOSFIR HIJAUAN PAKAN

ZOOTEC ◽  
2017 ◽  
Vol 37 (1) ◽  
pp. 167
Author(s):  
Rifa E. Ansiga ◽  
A. Rumambi ◽  
D. A. Kaligis ◽  
I. Mansur ◽  
W. Kaunang
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Gliricidia Sepium ◽  
Soil Samples ◽  
Isolation And Identification ◽  
Different Types ◽  
Forage Type ◽  
The Difference

EXPLORATION OF ARBUSCULAR MYCORRHIZAL (AM) FUNGI IN FORAGE RHIZOSPHERES. This study aimed to determine the diversity of Arbuscular Mycorrhizal Fungi (AMF) in several kinds of hybrid forages Rhizospheres, either in grasses or legumes. Soil samples were taken from three different locations, consisted of: Mapanget (forages type: Leucaena leucocepala, Sorghum varieties numbu, Penicettum purpureum cv. Mott), Tateli (forages type: calothyrsus Calliandra, Gliricidia sepium) and Campus of UNSRAT, Manado (forage type: King grass). The soil samples which taken from forages rhizospheres were sieved using Brundrett method and then centrifuged.  Thereafter, isolation and identification of spore were carried out based on spore morphology character, involves: shape, size, color, hyphae attachment, and ornament. Extraction and identification of spores on six types of rhizosphere were found 34 different types of AMF spores in shape and color. In grass, it was found three types of spores, i.e.: Glomus, Acaulospora, and Sclerocystis, meanwhile in leguminous just one type of spore was found, i.e.: Glomus. The difference of rhizosphere in grass and leguminosae resulted in different types of spores, where Sclerocystis and Acaulospora are found in grasses, on the contrary Sclerocystis and Acaulospora are not found in leguminosae. Based on the number of spores of AMF, it seemed that Glomus types found to have the most number, while Sclerocystis and Acaulospora had the lowest number of spores found. Key words: Exploration, Arbuscular Mycorrhizal Fungi (AMF), Grass, Legume, Spores

scholarly journals The Role of the external mycelial network of arbuscular mycorrhizal fungi: III. a study of nitrogen transfer between plants interconnected by a common mycelium

1998 ◽  
Vol 29 (4) ◽  
pp. 289-294 ◽  
Author(s):  
Marco A. Martins ◽  
Andre F. Cruz
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Plant Roots ◽  
Nitrogen Transfer ◽  
Nylon Mesh ◽  
Mycelium Network ◽  
Maize Plants ◽  
Mycelial Network

An experiment under greenhouse conditions was carried out to evaluate the relative contribuition of arbuscular mycorrhizal fungi (AMF) in the process of nitrogen transfer from cowpea to maize plants, using the isotope 15N. Special pots divided in three sections (A, B and C), were constructed and a nylon mesh screen of two diameters: 40µm (which allowed the AMF hyphae to pass but not the plant roots) or 1µm (which acted as a barrier to AM hyphae and plant roots) was inserted between the sections B and C. Section A had 25.5 mg of N/kg using (15NH4)2SO4 as N source. Two cowpea seedlings inoculated with Rhizobium sp. were transplanted with their root systems divided between the sections A and B. Ten days later, 2 seeds of maize were sown into the section C which was inoculated with Glomus etunicatum. Thirty-five days after transplanting, the maize plants were harvested. AMF inoculation increased dry weight and 15N and P content of maize plant shoots. Direct transfer of 15N via AMF hyphae was 21.2%; indirect transfer of 15N mediated by AMF mycelium network, was 9.6%, and indirect transfer not mediated by AM mycelium network , was 69.2%.

scholarly journals Acaulospora mellea and A. trappei, fungi new for Poland

2014 ◽  
Vol 34 (1) ◽  
pp. 41-50
Author(s):  
Janusz Błaszczykowski ◽  
Mariusz Tadych ◽  
Iwona Madej ◽  
Iwona Adamska ◽  
Beata Czerniawska ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples

Spores of <i>Acaulospora mellea</i> and <i>A. trappei</i> are described and illustrated. <i>Acaulospora mellea</i> occurs relatively infrequently in Poland. It was found in 30 of the more than 1300 soil samples examined representing different uncultivated and cultivated sites. Spores of <i>A. trappei</i> were only found in six field-collected soils. However, the occurrence of this fungus in many pot cultures with soils from various uncultivated and agricultural sites indicated that the species was common among arbuscular mycorrhizal fungi in Poland.

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