scholarly journals In Vitro Anti-Inflammatory, Anti-Oxidant, and Cytotoxic Activities of Four Curcuma Species and the Isolation of Compounds from Curcuma aromatica Rhizome

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 799 ◽  
Author(s):  
Aknarin Pintatum ◽  
Wisanu Maneerat ◽  
Emilie Logie ◽  
Emmy Tuenter ◽  
Maria E. Sakavitsi ◽  
...  
Keyword(s):  
Crude Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cytotoxic Activities ◽  
Ic50 Value ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Diphenyl Tetrazolium Bromide ◽  
Necrosis Factor

The genus Curcuma is part of the Zingiberaceae family, and many Curcuma species have been used as traditional medicine and cosmetics in Thailand. To find new cosmeceutical ingredients, the in vitro anti-inflammatory, anti-oxidant, and cytotoxic activities of four Curcuma species as well as the isolation of compounds from the most active crude extract (C. aromatica) were investigated. The crude extract of C. aromatica showed 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity with an IC50 value of 102.3 μg/mL. The cytotoxicity effect of C. aeruginosa, C. comosa, C. aromatica, and C. longa extracts assessed with the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay at 200 μg/mL were 12.1 ± 2.9, 14.4 ± 4.1, 28.6 ± 4.1, and 46.9 ± 8.6, respectively. C. aeruginosa and C. comosa presented apoptosis cells (57.7 ± 3.1% and 32.6 ± 2.2%, respectively) using the CytoTox-ONE™ assay. Different crude extracts or phytochemicals purified from C. aromatica were evaluated for their anti-inflammatory properties. The crude extract of C. aromatica showed the highest potential to inhibit NF-κB activity, followed by C. aeruginosa, C. comosa, and C. longa, respectively. Among the various purified phytochemicals curcumin, germacrone, curdione, zederone, and curcumenol significantly inhibited NF-κB activation in tumor necrosis factor (TNF) stimulated HaCaT keratinocytes. Of all compounds, curcumin was the most potent anti-inflammatory.

Evaluation of Anti-oxidant and Anti-pyretic activity of fruits of Garcinia pedunculata

2021 ◽  
pp. 2635-2638
Author(s):  
Mrinmoy Basak ◽  
Moksood Ahmed Laskar ◽  
Priyanka Goswami
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Traditional Use ◽  
Synthetic Drugs ◽  
Long Time ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Most of the synthetic drugs used at present as analgesic and anti-inflammatory agents cause many side effects and toxic effects. Many medicines of plant origin with analgesic and anti-inflammatory activity have been used since long time without adverse effects. The purpose of the study was to explore the anti-pyretic and anti-oxidant activity of fruits of Garcinia pedunculata. The fruit of G. pedunculata was collected from Shillong, Meghalaya and extraction is done by using methanol as a solvent. The methanol extract shows the presence of carbohydrate, glycoside, alkaloids, phenol and flavonoids. Acute anti-pyretic activity for the extract was investigated in pyrexia rat. Temperature level was determined after 1hours, 4 hours, 8 hours and 12 hours after giving the extract dose of 100mg/kg and 200mg/kg body weight, and it was found to produce a significant reduce in fever. DPPH radical scavenging activity (In-vitro anti-oxidant activity of G. pedunculata) of methanolic extract shows 38.31 as IC50 (µg/ml) whereas Ascorbic acid as standard in the same concentration shows 34.81 IC50 (µg/ml). The obtained result justified the traditional use of G. pedunculata as anti-pyretic and antioxidant purpose.

scholarly journals Evaluation of Anti-Oxidant and Anti-Pyretic Activity of Fruit of Garcenia Padunculata

2020 ◽  
Vol 8 (3) ◽  
pp. 77-80
Author(s):  
Basak Mrinmoy ◽  
Laskar Ahmed Moksood ◽  
Goswami Priyanka
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Traditional Use ◽  
Synthetic Drugs ◽  
Long Time ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Most of the synthetic drugs used at present as analgesic and anti-inflammatory agents cause many side effects and toxic effects. Many medicines of plant origin with analgesic and anti-inflammatory activity have been used since long time without adverse effects. The purpose of the study was to explore the anti-pyretic and anti-oxidant activity of fruits of Garcinia pedunculata. The fruit of G. pedunculatawas collected from Shillong, Meghalaya and extraction is done by using methanol as a solvent. The methanol extract shows the presence of carbohydrate, glycoside, alkaloids, phenol and flavonoids. Acute anti-pyretic activity for the extract was investigated in pyrexia rat. Temperature level was determined after 1hours, 4 hours, 8 hours and 12 hours after giving the extract dose of 100mg/kg and200mg/kg body weight, and it was found to produce a significant reduce in fever. DPPH radical scavenging activity (In-vitro anti-oxidant activity of G. pedunculata) of methanolic extract shows 38.31 as IC50 (µg/ml) whereas Ascorbic acid as standard in the same concentration shows34.81 IC50 (µg/ml). The obtained result justified the traditional use of G. pedunculata as anti-pyretic and antioxidant purpose.    

scholarly journals FORMULATION, CHARACTERIZATION AND EVALUATION OF ANTI-INFLAMMATORY AND ANTI-ANGIOGENIC ACTIVITIES OF MEMECYLAENE NANOEMULSION

2018 ◽  
Vol 8 (5-s) ◽  
pp. 126-131
Author(s):  
ND Rekha ◽  
Dattatri K. Nagesha ◽  
PH Rajasree ◽  
N Shruthi
Keyword(s):  
Zeta Potential ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vivo Model ◽  
Particle Size Range ◽  
Nitric Oxide Radical ◽  
Anti Oxidant ◽  
Anti Inflammatory

The present study was undertaken to formulate and evaluate the anti-inflammatory, anti-oxidant and anti-angiogenic activities of nanoemulsion of Memecylaene.  Memecylaene was isolated from the leaves of Memecylon malabaricum by using various chromatographic methods. An oil-in-water (O/W) nanoemulsion of Memecylaene was formulated by sonication method using sunflower oil (oil phase), Tween 80 (Surfactant) and Ethanol (co-surfactant). The prepared nanoemulsion was characterized for its droplet size, poly dispersity index and zeta potential. Stability studies were performed and the nanoemulsions were subjected to different biological activities. The formulated nanoemulsion had a particle size range of 52.02 nm to 59.47 nm and zeta potential of -1.27 mV. The enhanced activity of Memecylaene, encapsulated in O/W emulsions is evidenced by the inhibition of phospholipase (PLA2) enzyme and H+, K+ -ATPase and thus showing anti-inflammatory and anti-secretagogues effects. The in vitro anti-oxidant activity was evaluated by DPPH radical and Nitric oxide radical scavenging activity. Further, the inhibition of the growth of neo vessels formation in the in-vivo model system of chick chorioallantoic membrane (CAM) assay, which is angiogenesis dependent, was also observed. The above findings would help in understanding the putative potential of Memecylaene-loaded nanoemulsion as a therapeutic agent. Keywords: Anti-angiogenesis, Anti-oxidant, Gastric (H+ K+), Memecylaene, Nanoemulsion, Phospholipase A2 (PLA2).

In vitro Anti-oxidant and Anti-inflammatory Activity of the Aqueous and Ethanol Leaf Extracts of Clerodendrum phlomidis Linn. F.

2021 ◽  
pp. 5217-5221
Author(s):  
Krishnaveni R ◽  
Rajan S.
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
Scavenging Activity ◽  
Rbc Membrane ◽  
Clerodendrum Phlomidis ◽  
Anti Oxidant ◽  
Anti Inflammatory

Clerodendrum phlomid is was a common shrub of arid plains, low hills, and tropical deserts. It belongs to the family Verbenaceae. Many species of this genus have been documented in traditional system of medicine in India. The roots of the plant have been extensively studied in Ayurveda but in folk and traditional medicine, it is the leaf that finds more application in alleviating many diseases. Oxidation byproducts of metabolism was responsible for many pathophysiological conditions. Anti-oxidants are molecules that quench the free radicals. The present study aims at evaluating the in-vitro anti-oxidant and anti-inflammatory activity of the aqueous and ethanol leaf extract of Clerodendrum phlomidis. The anti-oxidant activity was evaluated by three in vitro models, namely the DPPH radical scavenging activity, hydroxyl radical scavenging activity and NO radical scavenging activity. The anti-inflammatory activity was evaluated by the RBC membrane Inhibition method. The aqueous extract was found to have better anti- oxidant activity than the ethanol extract in DPPH and NO radical scavenging activity. The RBC membrane inhibition method clearly states that the aqueous leaf extract of Clerodendrum phlomidis was found to have a comparatively an effective anti-inflammatory activity than ethanol extract.

scholarly journals Effects of Namya Kanom Jeen powder extracts on anti-oxidative and anti-inflammatory properties

2018 ◽  
Vol 8 (8) ◽  
pp. 379 ◽  
Author(s):  
Preeya Dat-arun ◽  
Rattana Leelawattana ◽  
Pavinee Chinachoti
Keyword(s):  
Functional Properties ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Water Extract ◽  
No Production ◽  
Scavenging Activity ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Anti Inflammation

Background: Spices and herbs are known to have anti-oxidant and anti-inflammatory properties. We studied their properties of Namya Kanom Jeen (NKJ), a spicy soup (Southern Thailand recipe) with water and ethanol (50% and 95%) extracts.Methods: This study aimed to assess functional properties of NKJ powder extract using in vitro model. These functional properties were anti-oxidant, α-amylase inhibition, and anti-inflammatory properties. Anti-oxidant activities were determined using free radical scavenging activity (DPPH) and Ferric Reducing Anti-oxidant Power (FRAP). Anti-inflammation effect was studied by measuring nitric oxide (NO) production inhibition on RAW264.7 macrophage cells after being exposed to lipopolysaccharide (LPS). Results: Water extract of NKJ powder demonstrated the highest activity in anti-inflammatory and anti-oxidant property by DPPH radical scavenging activity when compared with ethanol extract. Conclusion: NKJ powder extracts may ameliorate the oxidation and inflammation conditions which would be the combined effect of several bioactive compounds from many of the ingredients within these extracts.Keywords: Anti-oxidant; Anti-inflammation; Phenolic compound; Namya Kanom Jeen powder

α-Glucosidase Inhibition, Antioxidant and Docking Studies of Hydroxycoumarins and their Mono and Bis O-alkylated/acetylated Analogs

2018 ◽  
Vol 15 (2) ◽  
pp. 127-135 ◽  
Author(s):  
Parvesh Singh ◽  
Nomandla Ngcoya ◽  
Ramgopal Mopuri ◽  
Nagaraju Kerru ◽  
Neha Manhas ◽  
...  
Keyword(s):  
In Silico ◽  
Biological Activities ◽  
Wide Spectrum ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Catalytic Site ◽  
Docking Simulations ◽  
In Silico Docking ◽  
Anti Oxidant

Background: Diabetes Mellitus (DM) is a complex metabolic disease illustrated by abnormally high levels of plasma glucose or hyperglycaemia. Accordingly, several α-glucosidase inhibitors have been developed for the treatment of diabetes and other degenerative disorders. While, a coumarin ring has the privilege to represent numerous natural and synthetic compounds with a wide spectrum of biological activities e.g. anti-cancer, anti-HIV, anti-viral, anti-malarial, anti-microbial, anti-convulsant, anti-hypertensive properties. Besides this, coumarins have also shown potential to inhibit α-glucosidase leading to a generation of new promising antidiabetic agents. However, the testing of O-substituted coumarins for α-glucosidase inhibition has evaded the attention of medicinal chemists. Methods: For O-alkylation/acetylation reactions, the hydroxyl coumarins (A-B) initially activated by K2CO3 in dry DMF were reacted with variedly substituted haloalkanes at room temperature under nitrogen. The synthesized compounds were tested for their α-glucosidase (from Saccharomyces cerevisiae) inhibitory activity and anti-oxidant activity using DPPH radical scavenging activity. In silico docking simulations were conducted using CDocker module in DS (Accelrys) to explore the binding modes of the representative compounds in the catalytic site of α-glucosidase. Results: All the coumarin analogues (A1, B1, A2-A10, B2-B8) including their precursors (A-B) were evaluated for their in vitro α-glucosidase inhibition using acarbose as a standard inhibitor. All the mono O-alkylated coumarins (except A1) showed significant (p <0.05) α-glucosidase inhibition relative to the hydroxyl coumarin (A) with IC50 values ranging between 11.084±0.117 to 145.24± 29.22 µg/mL. Compound 7-(benzyloxy)-4, 5-dimethyl-2H-chromen-2-one (A9) bearing a benzyl group (Ph-CH2-) at position 7 showed a remarkable (p <0.05) increase in the activity (IC50 = 11.084±0.117 µg/mL), almost four-fold more than acarbose (IC50 = 40.578±5.999 µg/mL). The introduction of –NO2 group dramatically improved the anti-oxidant activity of coumarin, while the O-alkylation/acetylation decreased the activity. Conclusion: The present study describes the synthesis of functionalized coumarins and their evaluation for α-glucosidase inhibition and antioxidant activity under in vitro conditions. Based on IC50 data, the mono O-alkylated coumarins were observed to be stronger inhibitors of α-glucosidase with respect to their bis O-alkylated analogues. Coumarin (A9) bearing O-benzyloxy group displayed the strongest α-glucosidase inhibition, even higher than the standard inhibitor acarbose. The coumarin (A10) bearing –NO2 group showed the highest anti-oxidant activity amongst the synthesized compounds, almost comparable to the ascorbic acid. Finally, in silico docking simulations revealed the role of hydrogen bonding and hydrophobic forces in locking the compounds in catalytic site of α-glucosidase.

In vitro antioxidant activity and phytochemical analyses of Acalypha godseffiana (Euphobiaceae) leaf extracts

2021 ◽  
Vol 25 (1) ◽  
pp. 75-79
Author(s):  
S.O. Olubodun ◽  
G.E. Eriyamremu ◽  
M.E. Ayevbuomwan ◽  
C.I. Nzoputa
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anti Oxidant ◽  
In Vitro Antioxidant Activity

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

scholarly journals Antioxidant and Anti-Inflammatory Activities of Six Flavonoids from Smilax glabra Roxb

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5295
Author(s):  
Xinyu Zhao ◽  
Ruyi Chen ◽  
Yueyue Shi ◽  
Xiaoxi Zhang ◽  
Chongmei Tian ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Raw264.7 Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Preparative Hplc ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory

This study aimed to isolate, prepare and identify the main flavonoids from a standardized Smilax glabra flavonoids extract (SGF) using preparative HPLC, MS, 1H NMR and 13C NMR, determine the contents of these flavonoids using UPLC, then compare their pharmacological activities in vitro. We obtained six flavonoids from SGF: astilbin (18.10%), neoastilbin (11.04%), isoastilbin (5.03%), neoisoastilbin (4.09%), engeletin (2.58%) and (−)-epicatechin (1.77%). The antioxidant activity of six flavonoids were evaluated by determining the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and 2,2′-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS+) radical scavenging activity and ferric reducing antioxidant power (FRAP). In addition, the anti-inflammatory activity of six flavonoids were evaluated by determining the production of cytokines (IL-1β, IL-6), nitric oxide (NO) using enzyme linked immunosorbent assay and the NF-κB p65 expression using Western blotting in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The results showed that (−)-epicatechin, astilbin, neoastilbin, isoastilbin and neoisoastilbin had strong antioxidant activities, not only in DPPH and ABTS+ radicals scavenging capacities, but in FRAP system. Furthermore, all the six flavonoids could significantly inhibit the secretion of IL-1β, IL-6, NO (p < 0.01) and the protein expression of NF-κB p-p65 (p < 0.01) in LPS-stimulated RAW264.7 cells. This study preliminarily verified the antioxidant and anti-inflammatory activities of six flavonoids in S. glabra.

scholarly journals Behaviour of 9-Ethyl-9H-carbazole Hydrazone Derivatives Against Oxidant Systems

2019 ◽  
Vol 92 (1) ◽  
pp. 87-94 ◽  
Author(s):  
Cigdem Karaaslan ◽  
Hande Gurer-Orhan ◽  
Sibel Suzen ◽  
Luciano Saso ◽  
Omidreza Firuzi ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Amyloid Β ◽  
In Vitro Assays ◽  
Cytotoxic Activities ◽  
Scavenging Activity ◽  
Neuroprotective Effects ◽  
Neuronal Pc12 Cells

Antioxidants are helpful in prevention of several diseases related with oxidative stress including neurodegenerative disorders. In recent studies, carbazoles were given proof of promising antioxidant activities. In this article, 9-ethyl-9H-carbazole hydrazone derivatives were synthesized, characterized and their in vitro antioxidant activity and possible cytotoxic effects were investigated. Furthermore, protective effect of the synthesized derivatives against amyloid β-induced damage in PC12 neuronal cells was examined by using MTT assay. The newly synthesized carbazoles were found to have radical scavenging activity with a varying potency both in cell-free and cell-based in vitro assays. Several compounds, especially such as 3d and 3e, 3m and 3n bearing two halogen groups on the phenyl ring, were found to have cytotoxic activity. However, their cytotoxic activities were not higher than that of melatonin. Several compounds also significantly protected neuronal PC12 cells against amyloid β-induced damage, which can be defined as neuroprotective agents. (4-(2-((9-Ethyl-9H-carbazol-3-yl)methylene)hydrazinyl)benzonitrile) 3r was found as the most active compound with both radical scavenging activity and neuroprotective effects against amyloid β-induced damage. These findings might provide an alternative strategy for developing novel carbazole derivatives for management of neurodegenerative diseases, such as Alzheimer's disease.

scholarly journals Antioxidant Activities of the Leaf Extract and Fractions of Dryopteris filix-mas (L.) Schott could be Attributed to The Abundance of Polyphenol Compounds

2020 ◽  
Vol 9 (1) ◽  
pp. 1-6
Author(s):  
Earnest Oghenesuvwe Erhirhie ◽  
Emmanuel Emeka Ilodigwe ◽  
Daniel Lotanna Ajaghaku ◽  
Blessing Ogechukwu Umeokoli ◽  
Peter Maduabuchi Eze ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Bioactive Compound ◽  
Ethyl Acetate Fraction ◽  
In Vitro Assays ◽  
Anti Oxidant

Dryopteris filix mas (D filix-mas) is wildly used in ethnomedicine for the management of rheumatoid arthritis, wounds and other diseases. We investigated the anti-oxidant activities of its leaf extract, and chromatographic fractions. The ethanol leaf extract was partitioned into four fractions; n-hexane, ethyl acetate, n-butanol and water. Ferric reducing anti-oxidant power (FRAP), 1, 1-diphenyl-2-picrylhydrazil (DPPH) and nitric oxide (NO) scavenging in vitro assays were carried out on the extract and fractions at 6.25, 12.5, 25, 50, 100, 200, 400 and 800 µg/mL. The most active fraction (ethyl acetate fraction) was further purified using chromatographic techniques to isolate its major compound whose structure was elucidated using ID nuclear magnetic resonance (NMR) and mass spectrometry. The ethyl acetate fraction produced the highest free radical scavenging activity among the other fractions. The fraction (VLC-E7) from which the bioactive compound, quercetin-3-O-αL-rhamnopyranoside, was isolated had the best FRAP and DPPH scavenging activities with EC50 and IC50 values of 88.81 ± 3.41 and 26.87 ± 0.24 respectively more than the ethyl acetate fraction. This study revealed that the polyphenol flavonoid, quercetin-3-O-αL-rhamnopyranoside could be responsible for antioxidant activity of ethno-medicinal property of D filix-mas leaf.

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